FvH4_3g21430

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010677
Physical Location & Seq
Forward (+)
14519474 .. 14523295
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21430.t2

Sequence Viewer

Length: 753 bp
ATGAGCTGTAGCTCCGCCACTGGTATTGCAAATCGTCAACGTTCCAAATATTTGGCTCGAAAGAGAAAAAAAAAAAGAAAAAAAAAAAGAAGGATCACAGCAAAGTTGAAACACCAGAGACTCCAGAGGATCCCTGAAATGGCTACCAAAGTGTATATTGTGTACTATTCCATGTACGGACATGTAGAGAAGCTAGCAGAAGAAATTAAAAAGGGAGCTGCATCTGTGGAAGGAGTTGAAGCCACACTATGGCAGGTCCCTGAGACACTACAAGATGAGGTGCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCACGCCAAATGAACTTGCTGATGCAGATGGATTTGTATTTGGCTTTCCCACAAGATTTGGTATGATGGCTGCTCAATTTAAAGCATTTATGGACGCAACTGGGGGTCTATGGAGAACACAACAGCTTGCTGGAAAACCTGCTGGGATCTTTTACAGCACTGGATCTCAAGGTGGTGGACAAGAGACAACTGCATTGACTGCCATCACTCAGCTGGTTCACCACGGGATGATATTTGTTCCCATTGGATACACATTTGGAGCTGGCATGTTTGAAATGGAGAATATCAAGGGAGGAAGTCCATATGGTGCAGGAACATTTGCCGGGGATGGTTCAAGACAGCCAACTAAGCTTGAGCTCGAGCAAGCATTCCACCAAGGGAAATACATTGCCACCATCACAAAGAAGCTGAAGGGAGCTGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

251

Amino Acids

27.28

Weight (kDa)

9.87

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 6 - 134 2.3e-14 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 244, 472
AccB7I CCANNNNNTGG 1 cut(s) 249
AciI CCGC 1 cut(s) 15
AclI AACGTT 1 cut(s) 40
AclWI GGATC 5 cut(s) 101, 124, 137, 479, 496
AfaI GTAC 2 cut(s) 164, 176
AfiI CCNNNNNNNGG 2 cut(s) 139, 249
AflIII ACRYGT 1 cut(s) 181
AgsI TTSAA 4 cut(s) 109, 239, 599, 660
Alw21I GWGCWC 2 cut(s) 301, 684
Alw26I GTCTC 3 cut(s) 112, 257, 503
Alw44I GTGCAC 1 cut(s) 297
AlwI GGATC 5 cut(s) 101, 124, 137, 479, 496
Ama87I CYCGRG 1 cut(s) 683
ApaLI GTGCAC 1 cut(s) 297
ApeKI GCWGC 4 cut(s) 218, 395, 743, 746
AspS9I GGNCC 1 cut(s) 256
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 1 cut(s) 536
AsuNHI GCTAGC 1 cut(s) 193
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 256
BaeGI GKGCMC 1 cut(s) 301
BamHI GGATCC 1 cut(s) 129
BanII GRGCYC 1 cut(s) 684
Bbv12I GWGCWC 2 cut(s) 301, 684
BbvI GCAGC 4 cut(s) 205, 382, 730, 733
BccI CCATC 5 cut(s) 347, 385, 536, 647, 728
BciVI GTATCC 1 cut(s) 566
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 3 cut(s) 112, 257, 503
BfaI CTAG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 7
BfuAI ACCTGC 2 cut(s) 244, 472
BfuI GTATCC 1 cut(s) 566
BisI GCNGC 4 cut(s) 219, 396, 744, 747
BlsI GCNGC 4 cut(s) 220, 397, 745, 748
Bme1390I CCNGG 1 cut(s) 649
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 256
BmiI GGNNCC 2 cut(s) 131, 258
BmrFI CCNGG 1 cut(s) 649
BmrI ACTGGG 1 cut(s) 435
BmsI GCATC 3 cut(s) 230, 304, 337
BmtI GCTAGC 1 cut(s) 197
BmuI ACTGGG 1 cut(s) 435
BpmI CTGGAG 1 cut(s) 107
BpuEI CTTGAG 2 cut(s) 477, 698
BpuMI CCSGG 1 cut(s) 649
BsaJI CCNNGG 3 cut(s) 547, 648, 700
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 249
Bse1I ACTGG 3 cut(s) 25, 430, 490
Bse3DI GCAATG 1 cut(s) 711
BseDI CCNNGG 3 cut(s) 547, 648, 700
BseGI GGATG 2 cut(s) 558, 658
BseLI CCNNNNNNNGG 2 cut(s) 139, 249
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 2 cut(s) 252, 548
BseNI ACTGG 3 cut(s) 25, 430, 490
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 4 cut(s) 205, 382, 730, 733
BseYI CCCAGC 1 cut(s) 467
BsgI GTGCAG 1 cut(s) 654
BsiHKAI GWGCWC 2 cut(s) 301, 684
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 1 cut(s) 648
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 139, 249
BsmAI GTCTC 3 cut(s) 112, 257, 503
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 692
BsoBI CYCGRG 1 cut(s) 683
Bsp1286I GDGCHC 2 cut(s) 301, 684
Bsp143I GATC 4 cut(s) 93, 129, 471, 488
BspACI CCGC 1 cut(s) 15
BspCNI CTCAG 2 cut(s) 253, 547
BspLI GGNNCC 2 cut(s) 131, 258
BspMI ACCTGC 2 cut(s) 244, 472
BspOI GCTAGC 1 cut(s) 197
BspPI GGATC 5 cut(s) 101, 124, 137, 479, 496
BsrDI GCAATG 1 cut(s) 711
BsrI ACTGG 3 cut(s) 25, 430, 490
BssECI CCNNGG 3 cut(s) 547, 648, 700
BssMI GATC 4 cut(s) 93, 129, 471, 488
BssT1I CCWWGG 1 cut(s) 700
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 4 cut(s) 195, 453, 589, 690
BstDEI CTNAG 3 cut(s) 261, 534, 672
BstDSI CCRYGG 1 cut(s) 547
BstF5I GGATG 2 cut(s) 558, 658
BstKTI GATC 4 cut(s) 96, 132, 474, 491
BstMAI GTCTC 3 cut(s) 112, 257, 503
BstMBI GATC 4 cut(s) 93, 129, 471, 488
BstMWI GCNNNNNNNGC 2 cut(s) 524, 673
BstNSI RCATGY 2 cut(s) 185, 595
BstSCI CCNGG 1 cut(s) 647
BstSFI CTRYAG 1 cut(s) 7
BstSLI GKGCMC 1 cut(s) 301
BstV1I GCAGC 4 cut(s) 205, 382, 730, 733
BstX2I RGATCY 3 cut(s) 129, 471, 488
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 3 cut(s) 129, 471, 488
BsuI GTATCC 1 cut(s) 566
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 2 cut(s) 558, 658
BtsIMutI CAGTG 2 cut(s) 18, 483
BveI ACCTGC 2 cut(s) 244, 472
Cac8I GCNNGC 4 cut(s) 195, 453, 589, 690
Cfr13I GGNCC 1 cut(s) 256
CseI GACGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 163, 175
CspCI CAANNNNNGTGG 4 cut(s) 7, 42, 364, 399
CviAII CATG 3 cut(s) 172, 182, 592
CviQI GTAC 2 cut(s) 163, 175
DdeI CTNAG 3 cut(s) 261, 534, 672
DpnI GATC 4 cut(s) 95, 131, 473, 490
DpnII GATC 4 cut(s) 93, 129, 471, 488
DraI TTTAAA 1 cut(s) 406
EciI GGCGGA 1 cut(s) 4
Ecl136II GAGCTC 1 cut(s) 682
Eco130I CCWWGG 1 cut(s) 700
Eco24I GRGCYC 1 cut(s) 684
Eco47I GGWCC 1 cut(s) 256
Eco53kI GAGCTC 1 cut(s) 682
Eco88I CYCGRG 1 cut(s) 683
EcoICRI GAGCTC 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 256
EcoT14I CCWWGG 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 684
ErhI CCWWGG 1 cut(s) 700
FaeI CATG 3 cut(s) 175, 185, 595
FaqI GGGAC 1 cut(s) 242
FatI CATG 3 cut(s) 171, 181, 591
FauNDI CATATG 1 cut(s) 628
Fnu4HI GCNGC 4 cut(s) 219, 396, 744, 747
FokI GGATG 2 cut(s) 565, 665
FriOI GRGCYC 1 cut(s) 684
Fsp4HI GCNGC 4 cut(s) 219, 396, 744, 747
FspBI CTAG 1 cut(s) 194
GluI GCNGC 4 cut(s) 219, 396, 744, 747
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 107
HapII CCGG 1 cut(s) 648
HgaI GACGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 175, 185, 595
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 1 cut(s) 120
HpaII CCGG 1 cut(s) 648
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 5 cut(s) 38, 163, 299, 503, 544
Hpy188III TCNNGA 2 cut(s) 124, 660
Hpy8I GTNNAC 5 cut(s) 38, 163, 299, 503, 544
HpyAV CCTTC 3 cut(s) 84, 224, 730
HpyCH4IV ACGT 1 cut(s) 40
HpyCH4V TGCA 7 cut(s) 29, 221, 299, 317, 350, 518, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 673
HpyF3I CTNAG 3 cut(s) 261, 534, 672
HpySE526I ACGT 1 cut(s) 40
Hsp92II CATG 3 cut(s) 175, 185, 595
Kzo9I GATC 4 cut(s) 93, 129, 471, 488
LmnI GCTCC 4 cut(s) 17, 215, 584, 740
Lsp1109I GCAGC 4 cut(s) 205, 382, 730, 733
LweI GCATC 3 cut(s) 230, 304, 337
MaeI CTAG 1 cut(s) 194
MaeII ACGT 1 cut(s) 40
MalI GATC 4 cut(s) 95, 131, 473, 490
MboI GATC 4 cut(s) 93, 129, 471, 488
MboII GAAGA 1 cut(s) 212
MflI RGATCY 3 cut(s) 129, 471, 488
MhlI GDGCHC 2 cut(s) 301, 684
MluCI AATT 2 cut(s) 204, 401
MlyI GAGTC 1 cut(s) 114
MnlI CCTC 3 cut(s) 120, 271, 611
MseI TTAA 3 cut(s) 207, 405, 751
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 649
Mva1269I GAATGC 1 cut(s) 692
MwoI GCNNNNNNNGC 2 cut(s) 524, 673
NciI CCSGG 1 cut(s) 649
NdeI CATATG 1 cut(s) 628
NdeII GATC 4 cut(s) 93, 129, 471, 488
NheI GCTAGC 1 cut(s) 193
NlaIII CATG 3 cut(s) 175, 185, 595
NlaIV GGNNCC 2 cut(s) 131, 258
NspI RCATGY 2 cut(s) 185, 595
PaeR7I CTCGAG 1 cut(s) 683
PciI ACATGT 1 cut(s) 181
PctI GAATGC 1 cut(s) 692
PflMI CCANNNNNTGG 1 cut(s) 249
PkrI GCNGC 4 cut(s) 220, 397, 745, 748
PleI GAGTC 1 cut(s) 114
PpsI GAGTC 1 cut(s) 114
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 684
Psp1406I AACGTT 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 467
PspN4I GGNNCC 2 cut(s) 131, 258
PspPI GGNCC 1 cut(s) 256
PspPPI RGGWCCY 1 cut(s) 256
PspXI VCTCGAGB 1 cut(s) 683
PsuI RGATCY 3 cut(s) 129, 471, 488
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 2 cut(s) 164, 176
RsaNI GTAC 2 cut(s) 163, 175
SacI GAGCTC 1 cut(s) 684
SaqAI TTAA 3 cut(s) 207, 405, 751
SatI GCNGC 4 cut(s) 219, 396, 744, 747
Sau3AI GATC 4 cut(s) 93, 129, 471, 488
Sau96I GGNCC 1 cut(s) 256
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 1 cut(s) 649
SduI GDGCHC 2 cut(s) 301, 684
SfaNI GCATC 3 cut(s) 230, 304, 337
SfcI CTRYAG 1 cut(s) 7
Sfr274I CTCGAG 1 cut(s) 683
SinI GGWCC 1 cut(s) 256
SlaI CTCGAG 1 cut(s) 683
SmlI CTYRAG 3 cut(s) 492, 677, 683
SmoI CTYRAG 3 cut(s) 492, 677, 683
Sse9I AATT 2 cut(s) 204, 401
SsiI CCGC 1 cut(s) 15
SspI AATATT 1 cut(s) 50
SspMI CTAG 1 cut(s) 194
SstI GAGCTC 1 cut(s) 684
StyD4I CCNGG 1 cut(s) 647
StyI CCWWGG 1 cut(s) 700
TaiI ACGT 1 cut(s) 43
TaqI TCGA 2 cut(s) 58, 684
TasI AATT 2 cut(s) 204, 401
TatI WGTACW 1 cut(s) 162
Tru1I TTAA 3 cut(s) 207, 405, 751
Tru9I TTAA 3 cut(s) 207, 405, 751
TscAI CASTG 2 cut(s) 25, 490
TseI GCWGC 4 cut(s) 218, 395, 743, 746
TspDTI ATGAA 1 cut(s) 351
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 2 cut(s) 25, 490
Van91I CCANNNNNTGG 1 cut(s) 249
VneI GTGCAC 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 256
XceI RCATGY 2 cut(s) 185, 595
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XhoI CTCGAG 1 cut(s) 683
XspI CTAG 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.