pycom03g16450

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010677
Physical Location & Seq
Reverse (-)
17654214 .. 17655990
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16450.2

Sequence Viewer

Length: 708 bp
ATGTATGGTGGTCCCATGGAAAGAATGTGTAATACAAAAGAAAAGGGAGCTGTTATTGGCAACCAAAAATACTCATTCTGCCTGGAGGACTACAAATTGAATATTCTCCCTTATACTGTGTACTACTCCATGTATGGACATGTCAGGAGACTAGCAGAAGAAATAAAGAAAGGGGCTTCATCTGTCGAAGGTGTAGAAGCCACCCTATGGCAGGTCCCTGAGACACTCCCAGAGGAGGTGCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCTCGCCAAATGAACTTGCTGACGCAGATGGATTCATTTTTGGCTTCCCAACAAGATTTGGTATGATGGCTGCCCAATTTAAAGCCTTTCTGGATGCAACAGGCGGTCTATGGAGAACACAACAGCTTGCTGGAAAACCTGCTGGGATCTTTTACAGTACTGGATCTCAAGGTGGCGGCCAAGAGACTACTGCATTGACTGCCATCACTCAGCTGGTTCATCACGGGATGATATTTGTACCCATCGGTTACACATTCGGAGCTGGCATGTTTGAAATGGAGAATATCAAGGGCGGAAGTCCTTACGGTGCAGGAACGTATGCTGGGGACGGTTCAAGACAGCCATCTCAGCTTGAGCTGGAGCAAGCATTCCACCAAGGGAAGTACATTGCCACCATCACAAAGAAGCTCAAGGGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

236

Amino Acids

25.41

Weight (kDa)

6.44

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 202, 430
AccB7I CCANNNNNTGG 1 cut(s) 207
AciI CCGC 3 cut(s) 387, 459, 576
AclWI GGATC 2 cut(s) 437, 454
AcoI YGGCCR 1 cut(s) 460
AfaI GTAC 4 cut(s) 122, 442, 522, 668
AfiI CCNNNNNNNGG 3 cut(s) 207, 211, 235
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 3 cut(s) 100, 557, 618
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 8 cut(s) 50, 409, 496, 545, 634, 640, 691, 701
AluI AGCT 8 cut(s) 50, 409, 496, 545, 634, 640, 691, 701
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 3 cut(s) 142, 215, 461
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 2 cut(s) 437, 454
AoxI GGCC 1 cut(s) 460
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 2 cut(s) 353, 701
AspS9I GGNCC 2 cut(s) 11, 214
AvaII GGWCC 2 cut(s) 11, 214
BaeGI GKGCMC 1 cut(s) 259
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 2 cut(s) 340, 688
BccI CCATC 6 cut(s) 305, 343, 494, 533, 634, 686
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 3 cut(s) 142, 215, 461
BfaI CTAG 1 cut(s) 152
BfuAI ACCTGC 2 cut(s) 202, 430
BisI GCNGC 3 cut(s) 354, 460, 702
BlsI GCNGC 3 cut(s) 355, 461, 703
BmcAI AGTACT 1 cut(s) 442
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 2 cut(s) 11, 214
BmgT120I GGNCC 2 cut(s) 11, 214
BmiI GGNNCC 2 cut(s) 13, 216
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 2 cut(s) 262, 367
BpmI CTGGAG 2 cut(s) 104, 662
BpuEI CTTGAG 3 cut(s) 435, 656, 677
BsaJI CCNNGG 2 cut(s) 15, 658
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 211, 235
Bse1I ACTGG 1 cut(s) 448
Bse3DI GCAATG 1 cut(s) 669
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 2 cut(s) 15, 658
BseGI GGATG 2 cut(s) 382, 516
BseLI CCNNNNNNNGG 3 cut(s) 207, 211, 235
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 3 cut(s) 210, 506, 644
BseNI ACTGG 1 cut(s) 448
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 2 cut(s) 340, 688
BseYI CCCAGC 2 cut(s) 425, 605
BsgI GTGCAG 1 cut(s) 612
BshFI GGCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 259
BslFI GGGAC 2 cut(s) 200, 623
BslI CCNNNNNNNGG 3 cut(s) 207, 211, 235
BsmAI GTCTC 3 cut(s) 142, 215, 461
BsmFI GGGAC 2 cut(s) 200, 623
BsmI GAATGC 1 cut(s) 650
BsnI GGCC 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 2 cut(s) 429, 446
Bsp19I CCATGG 1 cut(s) 15
BspACI CCGC 3 cut(s) 387, 459, 576
BspANI GGCC 1 cut(s) 462
BspCNI CTCAG 3 cut(s) 211, 505, 643
BspLI GGNNCC 2 cut(s) 13, 216
BspMI ACCTGC 2 cut(s) 202, 430
BspPI GGATC 2 cut(s) 437, 454
BsrDI GCAATG 1 cut(s) 669
BsrI ACTGG 1 cut(s) 448
BssECI CCNNGG 2 cut(s) 15, 658
BssMI GATC 2 cut(s) 429, 446
BssT1I CCWWGG 2 cut(s) 15, 658
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 4 cut(s) 118, 440, 590, 614
BstAPI GCANNNNNTGC 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 411, 547, 648
BstDEI CTNAG 3 cut(s) 219, 492, 630
BstDSI CCRYGG 1 cut(s) 15
BstENI CCTNNNNNAGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 382, 516
BstKTI GATC 2 cut(s) 432, 449
BstMAI GTCTC 3 cut(s) 142, 215, 461
BstMBI GATC 2 cut(s) 429, 446
BstMWI GCNNNNNNNGC 2 cut(s) 482, 631
BstNI CCWGG 1 cut(s) 83
BstNSI RCATGY 2 cut(s) 143, 553
BstSCI CCNGG 1 cut(s) 81
BstSLI GKGCMC 1 cut(s) 259
BstV1I GCAGC 2 cut(s) 340, 688
BstX2I RGATCY 2 cut(s) 429, 446
BstYI RGATCY 2 cut(s) 429, 446
BsuRI GGCC 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 2 cut(s) 382, 516
BveI ACCTGC 2 cut(s) 202, 430
Cac8I GCNNGC 3 cut(s) 411, 547, 648
Cfr13I GGNCC 2 cut(s) 11, 214
CseI GACGC 1 cut(s) 314
Csp6I GTAC 4 cut(s) 121, 441, 521, 667
CviAII CATG 4 cut(s) 16, 130, 140, 550
CviQI GTAC 4 cut(s) 121, 441, 521, 667
DdeI CTNAG 3 cut(s) 219, 492, 630
DpnI GATC 2 cut(s) 431, 448
DpnII GATC 2 cut(s) 429, 446
DraI TTTAAA 1 cut(s) 364
EaeI YGGCCR 1 cut(s) 460
EciI GGCGGA 1 cut(s) 591
Eco130I CCWWGG 2 cut(s) 15, 658
Eco47I GGWCC 2 cut(s) 11, 214
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 214
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 15, 658
ErhI CCWWGG 2 cut(s) 15, 658
FaeI CATG 4 cut(s) 19, 133, 143, 553
FaqI GGGAC 2 cut(s) 200, 623
FatI CATG 4 cut(s) 15, 129, 139, 549
Fnu4HI GCNGC 3 cut(s) 354, 460, 702
FokI GGATG 2 cut(s) 389, 523
Fsp4HI GCNGC 3 cut(s) 354, 460, 702
FspBI CTAG 1 cut(s) 152
GluI GCNGC 3 cut(s) 354, 460, 702
GsaI CCCAGC 2 cut(s) 429, 609
GsuI CTGGAG 2 cut(s) 104, 662
HaeIII GGCC 1 cut(s) 462
HgaI GACGC 1 cut(s) 314
Hin1II CATG 4 cut(s) 19, 133, 143, 553
HinfI GANTC 1 cut(s) 315
Hpy166II GTNNAC 2 cut(s) 121, 257
Hpy188I TCNGA 1 cut(s) 542
Hpy188III TCNNGA 3 cut(s) 145, 374, 618
Hpy8I GTNNAC 2 cut(s) 121, 257
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 4 cut(s) 118, 440, 590, 614
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 5 cut(s) 257, 275, 380, 476, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 482, 631
HpyF3I CTNAG 3 cut(s) 219, 492, 630
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 4 cut(s) 19, 133, 143, 553
Kzo9I GATC 2 cut(s) 429, 446
LmnI GCTCC 4 cut(s) 47, 542, 643, 698
Lsp1109I GCAGC 2 cut(s) 340, 688
LweI GCATC 2 cut(s) 262, 367
MaeI CTAG 1 cut(s) 152
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 1 cut(s) 530
MalI GATC 2 cut(s) 431, 448
MboI GATC 2 cut(s) 429, 446
MboII GAAGA 1 cut(s) 170
MflI RGATCY 2 cut(s) 429, 446
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 2 cut(s) 95, 359
MnlI CCTC 3 cut(s) 79, 226, 229
MseI TTAA 2 cut(s) 363, 706
MspA1I CMGCKG 1 cut(s) 496
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 650
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 2 cut(s) 482, 631
NcoI CCATGG 1 cut(s) 15
NdeII GATC 2 cut(s) 429, 446
NlaIII CATG 4 cut(s) 19, 133, 143, 553
NlaIV GGNNCC 2 cut(s) 13, 216
NspI RCATGY 2 cut(s) 143, 553
PciI ACATGT 1 cut(s) 139
PctI GAATGC 1 cut(s) 650
PfeI GAWTC 1 cut(s) 315
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 3 cut(s) 355, 461, 703
PpuMI RGGWCCY 1 cut(s) 214
PscI ACATGT 1 cut(s) 139
Psp5II RGGWCCY 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 81
PspFI CCCAGC 2 cut(s) 425, 605
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 13, 216
PspPI GGNCC 2 cut(s) 11, 214
PspPPI RGGWCCY 1 cut(s) 214
PsuI RGATCY 2 cut(s) 429, 446
PvuII CAGCTG 1 cut(s) 496
RsaI GTAC 4 cut(s) 122, 442, 522, 668
RsaNI GTAC 4 cut(s) 121, 441, 521, 667
SaqAI TTAA 2 cut(s) 363, 706
SatI GCNGC 3 cut(s) 354, 460, 702
Sau3AI GATC 2 cut(s) 429, 446
Sau96I GGNCC 2 cut(s) 11, 214
ScaI AGTACT 1 cut(s) 442
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 1 cut(s) 259
SfaNI GCATC 2 cut(s) 262, 367
SinI GGWCC 2 cut(s) 11, 214
SmlI CTYRAG 3 cut(s) 450, 635, 692
SmoI CTYRAG 3 cut(s) 450, 635, 692
Sse9I AATT 2 cut(s) 95, 359
SsiI CCGC 3 cut(s) 387, 459, 576
SspI AATATT 1 cut(s) 103
SspMI CTAG 1 cut(s) 152
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 15, 658
TaaI ACNGT 4 cut(s) 118, 440, 590, 614
TaiI ACGT 1 cut(s) 602
TaqI TCGA 1 cut(s) 186
TasI AATT 2 cut(s) 95, 359
TatI WGTACW 3 cut(s) 120, 440, 666
TauI GCSGC 1 cut(s) 462
TfiI GAWTC 1 cut(s) 315
Tru1I TTAA 2 cut(s) 363, 706
Tru9I TTAA 2 cut(s) 363, 706
TseI GCWGC 2 cut(s) 353, 701
TspDTI ATGAA 4 cut(s) 168, 307, 309, 491
Van91I CCANNNNNTGG 1 cut(s) 207
VneI GTGCAC 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 11, 214
XagI CCTNNNNNAGG 1 cut(s) 209
XceI RCATGY 2 cut(s) 143, 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XspI CTAG 1 cut(s) 152
ZrmI AGTACT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.