RLG00000033728

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010677
Physical Location & Seq
Forward (+)
31457713 .. 31460659
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033728

Sequence Viewer

Length: 693 bp
ATGGCCTTTAGTGTGCTACTGTTGAAGTGGAGAAAATGGATTTACTTTGCTGAGGAAGTTCTGTTCAGACTGATGAATGAATCAGGGAATTTCGAATCCAAGTACTATTCCATGTATGGACATGTAGAGAAGCTAGCAGAAGAAATTAAAAAAGGAGCTGCATCTGTGGAGGGAGTAGAAGCCACACTATGGCAGGTCCCTGAGACACTACAAGATGAGGTGCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCACGCCAAATGAACTTGCTGATGCAGATGGATTTGTTTTTGGCTTTCCAACAAGATTTGGTATGATGGCTGCTCAGTTTAAAGCATTTCTGGATGCAACTGGAGGTCTATGGAGAACACAACAGCTTGCTGGAAAACCTGCTGGGATCTTTTACAGCACTGGATCTCAAGGTGGTGGACAAGAGACAACTGCATTGACTGCCATCACTCAGCTGGTTCACCATGGGATGATATTTGTACCCATTGGCTACACATTTGGAGCTGGCATGTTTGAAATGGAGAATATCAAGGGAGGAAGTCCATATGGTGCAGGAACTTTTGCCGGGGATGGTTCAAGACAGCCAACTAAGCTTGAGCTCGAGCAAGCATTCCACCAAGGGAAATACATTGCCACCATCACAAAGAAGCTCAAGGGAGCTGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

231

Amino Acids

25.0

Weight (kDa)

6.11

Isoelectric Point (pI)

32.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_1 PF00258 35 - 164 2.1e-07 Flavodoxin
FMN_red PF03358 43 - 171 3.7e-14 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 184, 412
AccB7I CCANNNNNTGG 1 cut(s) 189
AclWI GGATC 2 cut(s) 419, 436
AcsI RAATTY 1 cut(s) 88
AfaI GTAC 2 cut(s) 104, 504
AfiI CCNNNNNNNGG 1 cut(s) 189
AflIII ACRYGT 1 cut(s) 121
AgsI TTSAA 3 cut(s) 25, 539, 600
AloI GAACNNNNNNTCC 2 cut(s) 47, 79
AluBI AGCT 9 cut(s) 133, 158, 391, 478, 527, 616, 622, 673, 683
AluI AGCT 9 cut(s) 133, 158, 391, 478, 527, 616, 622, 673, 683
Alw21I GWGCWC 2 cut(s) 241, 624
Alw26I GTCTC 2 cut(s) 197, 443
Alw44I GTGCAC 1 cut(s) 237
AlwI GGATC 2 cut(s) 419, 436
Ama87I CYCGRG 1 cut(s) 623
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 237
ApeKI GCWGC 4 cut(s) 158, 335, 683, 686
ApoI RAATTY 1 cut(s) 88
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 1 cut(s) 589
AsuHPI GGTGA 1 cut(s) 476
AsuII TTCGAA 1 cut(s) 93
AsuNHI GCTAGC 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 623
AvaII GGWCC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 241
BanII GRGCYC 1 cut(s) 624
Bbv12I GWGCWC 2 cut(s) 241, 624
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 4 cut(s) 145, 322, 670, 673
BccI CCATC 5 cut(s) 287, 325, 476, 587, 668
BcnI CCSGG 1 cut(s) 589
BcoDI GTCTC 2 cut(s) 197, 443
BfaI CTAG 1 cut(s) 134
BfuAI ACCTGC 2 cut(s) 184, 412
BisI GCNGC 4 cut(s) 159, 336, 684, 687
BlsI GCNGC 4 cut(s) 160, 337, 685, 688
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 589
Bme18I GGWCC 1 cut(s) 196
BmeT110I CYCGRG 1 cut(s) 623
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 589
BmsI GCATC 4 cut(s) 170, 244, 277, 349
BmtI GCTAGC 1 cut(s) 137
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 51
Bpu14I TTCGAA 1 cut(s) 93
BpuEI CTTGAG 3 cut(s) 417, 638, 659
BpuMI CCSGG 1 cut(s) 589
BsaJI CCNNGG 3 cut(s) 487, 588, 640
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 2 cut(s) 370, 430
Bse3DI GCAATG 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 487, 588, 640
BseGI GGATG 3 cut(s) 364, 498, 598
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMI GCAATG 1 cut(s) 651
BseMII CTCAG 4 cut(s) 42, 192, 353, 488
BseNI ACTGG 2 cut(s) 370, 430
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 4 cut(s) 145, 322, 670, 673
BseYI CCCAGC 1 cut(s) 407
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 2 cut(s) 241, 624
BsiHKCI CYCGRG 1 cut(s) 623
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 197, 443
BsmFI GGGAC 1 cut(s) 182
BsmI GAATGC 1 cut(s) 632
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 623
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 241, 624
Bsp143I GATC 2 cut(s) 411, 428
Bsp19I CCATGG 1 cut(s) 487
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 4 cut(s) 43, 193, 352, 487
BspLI GGNNCC 1 cut(s) 198
BspMI ACCTGC 2 cut(s) 184, 412
BspOI GCTAGC 1 cut(s) 137
BspPI GGATC 2 cut(s) 419, 436
BspT104I TTCGAA 1 cut(s) 93
BsrDI GCAATG 1 cut(s) 651
BsrI ACTGG 2 cut(s) 370, 430
BssECI CCNNGG 3 cut(s) 487, 588, 640
BssMI GATC 2 cut(s) 411, 428
BssT1I CCWWGG 2 cut(s) 487, 640
Bst4CI ACNGT 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 464
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 4 cut(s) 135, 393, 529, 630
BstDEI CTNAG 5 cut(s) 51, 201, 339, 474, 612
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 3 cut(s) 364, 498, 598
BstKTI GATC 2 cut(s) 414, 431
BstMAI GTCTC 2 cut(s) 197, 443
BstMBI GATC 2 cut(s) 411, 428
BstMWI GCNNNNNNNGC 2 cut(s) 464, 613
BstNSI RCATGY 2 cut(s) 125, 535
BstSCI CCNGG 1 cut(s) 587
BstSLI GKGCMC 1 cut(s) 241
BstV1I GCAGC 4 cut(s) 145, 322, 670, 673
BstX2I RGATCY 2 cut(s) 411, 428
BstYI RGATCY 2 cut(s) 411, 428
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 487
BtsCI GGATG 3 cut(s) 364, 498, 598
BtsIMutI CAGTG 1 cut(s) 423
BveI ACCTGC 2 cut(s) 184, 412
Cac8I GCNNGC 4 cut(s) 135, 393, 529, 630
Cfr13I GGNCC 1 cut(s) 196
Csp6I GTAC 2 cut(s) 103, 503
CviAII CATG 4 cut(s) 112, 122, 488, 532
CviQI GTAC 2 cut(s) 103, 503
DdeI CTNAG 5 cut(s) 51, 201, 339, 474, 612
DpnI GATC 2 cut(s) 413, 430
DpnII GATC 2 cut(s) 411, 428
DraI TTTAAA 1 cut(s) 346
Ecl136II GAGCTC 1 cut(s) 622
Eco130I CCWWGG 2 cut(s) 487, 640
Eco24I GRGCYC 1 cut(s) 624
Eco47I GGWCC 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 622
Eco88I CYCGRG 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 622
EcoO109I RGGNCCY 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 487, 640
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 2 cut(s) 487, 640
FaeI CATG 4 cut(s) 115, 125, 491, 535
FaqI GGGAC 1 cut(s) 182
FatI CATG 4 cut(s) 111, 121, 487, 531
FauNDI CATATG 1 cut(s) 568
Fnu4HI GCNGC 4 cut(s) 159, 336, 684, 687
FokI GGATG 3 cut(s) 371, 505, 605
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 4 cut(s) 159, 336, 684, 687
FspBI CTAG 1 cut(s) 134
GluI GCNGC 4 cut(s) 159, 336, 684, 687
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 588
Hin1II CATG 4 cut(s) 115, 125, 491, 535
HindIII AAGCTT 1 cut(s) 614
HinfI GANTC 2 cut(s) 80, 95
HpaII CCGG 1 cut(s) 588
HphI GGTGA 1 cut(s) 476
Hpy166II GTNNAC 3 cut(s) 239, 443, 484
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 2 cut(s) 356, 600
Hpy8I GTNNAC 3 cut(s) 239, 443, 484
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4V TGCA 7 cut(s) 161, 239, 257, 290, 362, 458, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 613
HpyF3I CTNAG 5 cut(s) 51, 201, 339, 474, 612
Hsp92II CATG 4 cut(s) 115, 125, 491, 535
Kzo9I GATC 2 cut(s) 411, 428
LmnI GCTCC 3 cut(s) 155, 524, 680
Lsp1109I GCAGC 4 cut(s) 145, 322, 670, 673
LweI GCATC 4 cut(s) 170, 244, 277, 349
MaeI CTAG 1 cut(s) 134
MalI GATC 2 cut(s) 413, 430
MboI GATC 2 cut(s) 411, 428
MboII GAAGA 1 cut(s) 152
MflI RGATCY 2 cut(s) 411, 428
MhlI GDGCHC 2 cut(s) 241, 624
MluCI AATT 2 cut(s) 88, 144
MmeI TCCRAC 1 cut(s) 338
MnlI CCTC 5 cut(s) 46, 163, 211, 362, 551
MseI TTAA 3 cut(s) 147, 345, 691
MspA1I CMGCKG 1 cut(s) 478
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 1 cut(s) 589
Mva1269I GAATGC 1 cut(s) 632
MwoI GCNNNNNNNGC 2 cut(s) 464, 613
NciI CCSGG 1 cut(s) 589
NcoI CCATGG 1 cut(s) 487
NdeI CATATG 1 cut(s) 568
NdeII GATC 2 cut(s) 411, 428
NheI GCTAGC 1 cut(s) 133
NlaIII CATG 4 cut(s) 115, 125, 491, 535
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 2 cut(s) 125, 535
NspV TTCGAA 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 623
PciI ACATGT 1 cut(s) 121
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 2 cut(s) 80, 95
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 4 cut(s) 160, 337, 685, 688
PpuMI RGGWCCY 1 cut(s) 196
PscI ACATGT 1 cut(s) 121
Psp124BI GAGCTC 1 cut(s) 624
Psp5II RGGWCCY 1 cut(s) 196
PspFI CCCAGC 1 cut(s) 407
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 196
PspPPI RGGWCCY 1 cut(s) 196
PspXI VCTCGAGB 1 cut(s) 623
PsuI RGATCY 2 cut(s) 411, 428
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 2 cut(s) 104, 504
RsaNI GTAC 2 cut(s) 103, 503
SacI GAGCTC 1 cut(s) 624
SaqAI TTAA 3 cut(s) 147, 345, 691
SatI GCNGC 4 cut(s) 159, 336, 684, 687
Sau3AI GATC 2 cut(s) 411, 428
Sau96I GGNCC 1 cut(s) 196
ScaI AGTACT 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 589
SduI GDGCHC 2 cut(s) 241, 624
SfaNI GCATC 4 cut(s) 170, 244, 277, 349
Sfr274I CTCGAG 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 1 cut(s) 196
SlaI CTCGAG 1 cut(s) 623
SmlI CTYRAG 4 cut(s) 432, 617, 623, 674
SmoI CTYRAG 4 cut(s) 432, 617, 623, 674
Sse9I AATT 2 cut(s) 88, 144
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 1 cut(s) 587
StyI CCWWGG 2 cut(s) 487, 640
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 2 cut(s) 93, 624
TasI AATT 2 cut(s) 88, 144
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 80, 95
Tru1I TTAA 3 cut(s) 147, 345, 691
Tru9I TTAA 3 cut(s) 147, 345, 691
TscAI CASTG 1 cut(s) 430
TseI GCWGC 4 cut(s) 158, 335, 683, 686
TspDTI ATGAA 3 cut(s) 89, 93, 291
TspRI CASTG 1 cut(s) 430
Van91I CCANNNNNTGG 1 cut(s) 189
VneI GTGCAC 1 cut(s) 237
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 88
XceI RCATGY 2 cut(s) 125, 535
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XhoI CTCGAG 1 cut(s) 623
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.