Rh5AG250600

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010677
Physical Location & Seq
Forward (+)
31971398 .. 31976095
4698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG250600.1

Sequence Viewer

Length: 582 bp
ATGTATGGACATGTAGAGAAGCTAGCAGAAGAAATTAAAAAAGGAGCTGCATCTGTGGAGGGAGTAGAAGCCACACTATGGCAGGTCCCTGAGACACTACAAGATGAGGTGCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCACACCAAATGAACTTGCTGATGCAGATGGATTTGTTTTTGGCTTTCCAACAAGATTTGGTATGATGGCTGCTCAGTTTAAAGCATTTCTGGATGCAACTGGAGGTCTATGGAGAACACAACAGCTTGCTGGAAAACCTGCTGGGATCTTTTACAGCACTGGATCTCAAGGTGGTGGACAAGAGACAACTGCTTTGACTGCCATCACTCAGCTGGTTCACCATGGGATGATATTTGTACCCATTGGCTACACATTTGGAGCTGGCATGTTTGAAATGGAGAATATCAAAGGAGGAAGTCCATATGGTGCAGGAACTTTTGCCGGGGATGGTTCAAGACAGCCAACTAAGCTTGAGCTGGAGCAAGCATTCCACCAAGGGAAATACATTGCCACCATCACAAAGAAGCTCAAGGGAGCTGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

193

Amino Acids

20.42

Weight (kDa)

5.8

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMN_red PF03358 6 - 134 2.4e-14 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 73, 301
AccB7I CCANNNNNTGG 1 cut(s) 78
AclWI GGATC 2 cut(s) 308, 325
AfaI GTAC 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 78
AflIII ACRYGT 1 cut(s) 10
AgsI TTSAA 2 cut(s) 428, 489
AluBI AGCT 9 cut(s) 22, 47, 280, 367, 416, 505, 511, 562, 572
AluI AGCT 9 cut(s) 22, 47, 280, 367, 416, 505, 511, 562, 572
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 86, 332
Alw44I GTGCAC 1 cut(s) 126
AlwI GGATC 2 cut(s) 308, 325
ApaLI GTGCAC 1 cut(s) 126
ApeKI GCWGC 4 cut(s) 47, 224, 572, 575
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 478
AsuHPI GGTGA 1 cut(s) 365
AsuNHI GCTAGC 1 cut(s) 22
AvaII GGWCC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 4 cut(s) 34, 211, 559, 562
BccI CCATC 5 cut(s) 176, 214, 365, 476, 557
BcnI CCSGG 1 cut(s) 478
BcoDI GTCTC 2 cut(s) 86, 332
BfaI CTAG 1 cut(s) 23
BfuAI ACCTGC 2 cut(s) 73, 301
BisI GCNGC 4 cut(s) 48, 225, 573, 576
BlsI GCNGC 4 cut(s) 49, 226, 574, 577
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 4 cut(s) 59, 133, 166, 238
BmtI GCTAGC 1 cut(s) 26
BpmI CTGGAG 2 cut(s) 276, 533
BpuEI CTTGAG 3 cut(s) 306, 527, 548
BpuMI CCSGG 1 cut(s) 478
BsaJI CCNNGG 3 cut(s) 376, 477, 529
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 259, 319
Bse3DI GCAATG 1 cut(s) 540
BseDI CCNNGG 3 cut(s) 376, 477, 529
BseGI GGATG 3 cut(s) 253, 387, 487
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 3 cut(s) 81, 242, 377
BseNI ACTGG 2 cut(s) 259, 319
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 4 cut(s) 34, 211, 559, 562
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 477
BslFI GGGAC 1 cut(s) 71
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 2 cut(s) 86, 332
BsmFI GGGAC 1 cut(s) 71
BsmI GAATGC 1 cut(s) 521
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 2 cut(s) 300, 317
Bsp19I CCATGG 1 cut(s) 376
BspCNI CTCAG 3 cut(s) 82, 241, 376
BspLI GGNNCC 1 cut(s) 87
BspMI ACCTGC 2 cut(s) 73, 301
BspOI GCTAGC 1 cut(s) 26
BspPI GGATC 2 cut(s) 308, 325
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 2 cut(s) 259, 319
BssECI CCNNGG 3 cut(s) 376, 477, 529
BssMI GATC 2 cut(s) 300, 317
BssT1I CCWWGG 2 cut(s) 376, 529
BstC8I GCNNGC 4 cut(s) 24, 282, 418, 519
BstDEI CTNAG 4 cut(s) 90, 228, 363, 501
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 3 cut(s) 253, 387, 487
BstKTI GATC 2 cut(s) 303, 320
BstMAI GTCTC 2 cut(s) 86, 332
BstMBI GATC 2 cut(s) 300, 317
BstMWI GCNNNNNNNGC 2 cut(s) 353, 502
BstNSI RCATGY 2 cut(s) 14, 424
BstSCI CCNGG 1 cut(s) 476
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 4 cut(s) 34, 211, 559, 562
BstX2I RGATCY 2 cut(s) 300, 317
BstYI RGATCY 2 cut(s) 300, 317
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 3 cut(s) 253, 387, 487
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 2 cut(s) 73, 301
Cac8I GCNNGC 4 cut(s) 24, 282, 418, 519
Cfr13I GGNCC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 392
CviAII CATG 3 cut(s) 11, 377, 421
CviQI GTAC 1 cut(s) 392
DdeI CTNAG 4 cut(s) 90, 228, 363, 501
DpnI GATC 2 cut(s) 302, 319
DpnII GATC 2 cut(s) 300, 317
DraI TTTAAA 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 376, 529
Eco47I GGWCC 1 cut(s) 85
EcoO109I RGGNCCY 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 376, 529
ErhI CCWWGG 2 cut(s) 376, 529
FaeI CATG 3 cut(s) 14, 380, 424
FaiI YATR 9 cut(s) 6, 12, 79, 218, 265, 378, 422, 457, 459
FaqI GGGAC 1 cut(s) 71
FatI CATG 3 cut(s) 10, 376, 420
FauNDI CATATG 1 cut(s) 457
Fnu4HI GCNGC 4 cut(s) 48, 225, 573, 576
FokI GGATG 3 cut(s) 260, 394, 494
Fsp4HI GCNGC 4 cut(s) 48, 225, 573, 576
FspBI CTAG 1 cut(s) 23
GluI GCNGC 4 cut(s) 48, 225, 573, 576
GsaI CCCAGC 1 cut(s) 300
GsuI CTGGAG 2 cut(s) 276, 533
HapII CCGG 1 cut(s) 477
Hin1II CATG 3 cut(s) 14, 380, 424
HindIII AAGCTT 1 cut(s) 503
HpaII CCGG 1 cut(s) 477
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 3 cut(s) 128, 332, 373
Hpy188III TCNNGA 2 cut(s) 245, 489
Hpy8I GTNNAC 3 cut(s) 128, 332, 373
HpyCH4V TGCA 6 cut(s) 50, 128, 146, 179, 251, 464
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 502
HpyF3I CTNAG 4 cut(s) 90, 228, 363, 501
Hsp92II CATG 3 cut(s) 14, 380, 424
Kzo9I GATC 2 cut(s) 300, 317
LmnI GCTCC 4 cut(s) 44, 413, 514, 569
Lsp1109I GCAGC 4 cut(s) 34, 211, 559, 562
LweI GCATC 4 cut(s) 59, 133, 166, 238
MaeI CTAG 1 cut(s) 23
MalI GATC 2 cut(s) 302, 319
MboI GATC 2 cut(s) 300, 317
MboII GAAGA 1 cut(s) 41
MflI RGATCY 2 cut(s) 300, 317
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 227
MnlI CCTC 4 cut(s) 52, 100, 251, 440
MseI TTAA 3 cut(s) 36, 234, 580
MspA1I CMGCKG 1 cut(s) 367
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 1 cut(s) 478
Mva1269I GAATGC 1 cut(s) 521
MwoI GCNNNNNNNGC 2 cut(s) 353, 502
NciI CCSGG 1 cut(s) 478
NcoI CCATGG 1 cut(s) 376
NdeI CATATG 1 cut(s) 457
NdeII GATC 2 cut(s) 300, 317
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 3 cut(s) 14, 380, 424
NlaIV GGNNCC 1 cut(s) 87
NspI RCATGY 2 cut(s) 14, 424
PciI ACATGT 1 cut(s) 10
PctI GAATGC 1 cut(s) 521
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 4 cut(s) 49, 226, 574, 577
PpuMI RGGWCCY 1 cut(s) 85
PscI ACATGT 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 85
PspFI CCCAGC 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 1 cut(s) 85
PspPPI RGGWCCY 1 cut(s) 85
PsuI RGATCY 2 cut(s) 300, 317
PvuII CAGCTG 1 cut(s) 367
RsaI GTAC 1 cut(s) 393
RsaNI GTAC 1 cut(s) 392
SaqAI TTAA 3 cut(s) 36, 234, 580
SatI GCNGC 4 cut(s) 48, 225, 573, 576
Sau3AI GATC 2 cut(s) 300, 317
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 478
SduI GDGCHC 1 cut(s) 130
SfaNI GCATC 4 cut(s) 59, 133, 166, 238
SinI GGWCC 1 cut(s) 85
SmlI CTYRAG 3 cut(s) 321, 506, 563
SmoI CTYRAG 3 cut(s) 321, 506, 563
Sse9I AATT 1 cut(s) 33
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 2 cut(s) 376, 529
TasI AATT 1 cut(s) 33
Tru1I TTAA 3 cut(s) 36, 234, 580
Tru9I TTAA 3 cut(s) 36, 234, 580
TscAI CASTG 1 cut(s) 319
TseI GCWGC 4 cut(s) 47, 224, 572, 575
TspDTI ATGAA 1 cut(s) 180
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 78
VneI GTGCAC 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 85
XceI RCATGY 2 cut(s) 14, 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.