RchiOBHm_Chr5g0036861

Minor allergen Alt a

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010677
Physical Location & Seq
Forward (+)
31142938 .. 31146811
3874 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31565

Sequence Viewer

Length: 615 bp
ATGGCTACCAAAGTGTATATTGTGTACTATTCCATGTATGGACATGTAGAGAAGCTAGCTGAAGAAATTAAAAAAGGAGCTGCATCTGTGGAGGGAGTAGAAGCCACACTATGGCAGGTCCCTGAGACACTACAAGATGAGGTGCTTGGGAAAATGAGTGCACCACCAAAGAGTGATGCACCAATCATCACACCAAATGAACTTGCTGATGCAGATGGATTTGTTTTTGGCTTTCCAACAAGATTTGGTATGATGGCTGCTCAGTTTAAAGCATTTCTGGATGCAACTGGAGGTCTATGGAGAACACAACAGCTTGCTGGAAAACCTGCTGGGATCTTTTACAGCACTGGATCTCAAGGTGGTGGACAAGAGACAACTGCTTTGACTGCCATCACTCAGCTGGTTCACCATGGGATGATATTTGTACCCATTGGCTACACATTTGGAGCTGGCATGTTTGAAATGGAGAATATCAAAGGAGGAAGTCCATATGGTGCAGGAACTTTTGCCGGGGATGGTTCAAGACAGCCAACTAAGCTTGAGCTGGAGCAAGCATTCCACCAAGGGAAATACATTGCCACCATCACAAAGAAGCTCAAGGGAGCTACTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00130 ko01110 map00130 map01110
Pfam Domains
Protein Families
FMN_red

Protein Analysis

204

Amino Acids

21.77

Weight (kDa)

6.09

Isoelectric Point (pI)

32.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Flavodoxin_2 PF02525 4 - 130 5.1e-06 Flavodoxin-like fold
Flavodoxin_1 PF00258 7 - 138 9.7e-09 Flavodoxin
FMN_red PF03358 17 - 145 2.2e-14 NADPH-dependent FMN reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27270 AT4G27270 AT4G27270 AT4G27270
fragaria_vesca FvH4_3g21430 FvH4_3g21430
malus_domestica MD11G1230800.v1.1
prunus_persica Prupe.4G196500_v2.0.a1
pyrus_communis pycom03g16450 pycom11g20440
rosa_chinensis RchiOBHm_Chr5g0036861
rosa_laevigata RLG00000033728
rosa_multiflora Rmu_sc0000399.1_g000016
rosa_roxburghii Rroxscaffold_1G00044140
rosa_rugosa Rorug05G0158000
rosa_samantha Rh5AG250600 Rh5BG252200 Rh5CG284200 Rh5DG260700
rosa_wichuraiana Rw5G023050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 106, 334
AccB7I CCANNNNNTGG 1 cut(s) 111
AclWI GGATC 2 cut(s) 341, 358
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 26, 426
AfiI CCNNNNNNNGG 1 cut(s) 111
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 2 cut(s) 461, 522
Alw21I GWGCWC 1 cut(s) 163
Alw26I GTCTC 2 cut(s) 119, 365
Alw44I GTGCAC 1 cut(s) 159
AlwI GGATC 2 cut(s) 341, 358
ApaLI GTGCAC 1 cut(s) 159
ApeKI GCWGC 2 cut(s) 80, 257
ArsI GACNNNNNNTTYG 2 cut(s) 364, 396
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 1 cut(s) 398
AsuNHI GCTAGC 1 cut(s) 55
AvaII GGWCC 1 cut(s) 118
BaeGI GKGCMC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 163
BbvI GCAGC 2 cut(s) 67, 244
BccI CCATC 5 cut(s) 209, 247, 398, 509, 590
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 2 cut(s) 119, 365
BfaI CTAG 1 cut(s) 56
BfuAI ACCTGC 2 cut(s) 106, 334
BisI GCNGC 2 cut(s) 81, 258
BlsI GCNGC 2 cut(s) 82, 259
Bme1390I CCNGG 1 cut(s) 511
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 511
BmsI GCATC 4 cut(s) 92, 166, 199, 271
BmtI GCTAGC 1 cut(s) 59
BpmI CTGGAG 2 cut(s) 309, 566
BpuEI CTTGAG 3 cut(s) 339, 560, 581
BpuMI CCSGG 1 cut(s) 511
BsaJI CCNNGG 3 cut(s) 409, 510, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 2 cut(s) 292, 352
Bse3DI GCAATG 1 cut(s) 573
BseDI CCNNGG 3 cut(s) 409, 510, 562
BseGI GGATG 3 cut(s) 286, 420, 520
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 3 cut(s) 114, 275, 410
BseNI ACTGG 2 cut(s) 292, 352
BseSI GKGCMC 1 cut(s) 163
BseXI GCAGC 2 cut(s) 67, 244
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 516
BsiHKAI GWGCWC 1 cut(s) 163
BsiSI CCGG 1 cut(s) 510
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 119, 365
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 1 cut(s) 554
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 2 cut(s) 333, 350
Bsp19I CCATGG 1 cut(s) 409
BspCNI CTCAG 3 cut(s) 115, 274, 409
BspLI GGNNCC 1 cut(s) 120
BspMI ACCTGC 2 cut(s) 106, 334
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 341, 358
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 2 cut(s) 292, 352
BssECI CCNNGG 3 cut(s) 409, 510, 562
BssMI GATC 2 cut(s) 333, 350
BssT1I CCWWGG 2 cut(s) 409, 562
BstC8I GCNNGC 4 cut(s) 57, 315, 451, 552
BstDEI CTNAG 4 cut(s) 123, 261, 396, 534
BstDSI CCRYGG 1 cut(s) 409
BstF5I GGATG 3 cut(s) 286, 420, 520
BstKTI GATC 2 cut(s) 336, 353
BstMAI GTCTC 2 cut(s) 119, 365
BstMBI GATC 2 cut(s) 333, 350
BstMWI GCNNNNNNNGC 2 cut(s) 386, 535
BstNSI RCATGY 2 cut(s) 47, 457
BstSCI CCNGG 1 cut(s) 509
BstSLI GKGCMC 1 cut(s) 163
BstV1I GCAGC 2 cut(s) 67, 244
BstX2I RGATCY 2 cut(s) 333, 350
BstYI RGATCY 2 cut(s) 333, 350
BtgI CCRYGG 1 cut(s) 409
BtsCI GGATG 3 cut(s) 286, 420, 520
BtsIMutI CAGTG 1 cut(s) 345
BveI ACCTGC 2 cut(s) 106, 334
Cac8I GCNNGC 4 cut(s) 57, 315, 451, 552
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 25, 425
CviAII CATG 4 cut(s) 34, 44, 410, 454
CviQI GTAC 2 cut(s) 25, 425
DdeI CTNAG 4 cut(s) 123, 261, 396, 534
DpnI GATC 2 cut(s) 335, 352
DpnII GATC 2 cut(s) 333, 350
DraI TTTAAA 1 cut(s) 268
Eco130I CCWWGG 2 cut(s) 409, 562
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 409, 562
ErhI CCWWGG 2 cut(s) 409, 562
FaeI CATG 4 cut(s) 37, 47, 413, 457
FaqI GGGAC 1 cut(s) 104
FatI CATG 4 cut(s) 33, 43, 409, 453
FauNDI CATATG 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 81, 258
FokI GGATG 3 cut(s) 293, 427, 527
Fsp4HI GCNGC 2 cut(s) 81, 258
FspBI CTAG 1 cut(s) 56
GluI GCNGC 2 cut(s) 81, 258
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 2 cut(s) 309, 566
HapII CCGG 1 cut(s) 510
Hin1II CATG 4 cut(s) 37, 47, 413, 457
HindIII AAGCTT 1 cut(s) 536
HpaII CCGG 1 cut(s) 510
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 4 cut(s) 25, 161, 365, 406
Hpy188III TCNNGA 2 cut(s) 278, 522
Hpy8I GTNNAC 4 cut(s) 25, 161, 365, 406
HpyCH4V TGCA 6 cut(s) 83, 161, 179, 212, 284, 497
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 535
HpyF3I CTNAG 4 cut(s) 123, 261, 396, 534
Hsp92II CATG 4 cut(s) 37, 47, 413, 457
Kzo9I GATC 2 cut(s) 333, 350
LmnI GCTCC 4 cut(s) 77, 446, 547, 602
Lsp1109I GCAGC 2 cut(s) 67, 244
LweI GCATC 4 cut(s) 92, 166, 199, 271
MaeI CTAG 1 cut(s) 56
MalI GATC 2 cut(s) 335, 352
MboI GATC 2 cut(s) 333, 350
MboII GAAGA 1 cut(s) 74
MflI RGATCY 2 cut(s) 333, 350
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 1 cut(s) 66
MmeI TCCRAC 1 cut(s) 260
MnlI CCTC 4 cut(s) 85, 133, 284, 473
MseI TTAA 3 cut(s) 69, 267, 613
MspA1I CMGCKG 1 cut(s) 400
MspI CCGG 1 cut(s) 510
MspR9I CCNGG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 554
MwoI GCNNNNNNNGC 2 cut(s) 386, 535
NciI CCSGG 1 cut(s) 511
NcoI CCATGG 1 cut(s) 409
NdeI CATATG 1 cut(s) 490
NdeII GATC 2 cut(s) 333, 350
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 4 cut(s) 37, 47, 413, 457
NlaIV GGNNCC 1 cut(s) 120
NspI RCATGY 2 cut(s) 47, 457
PciI ACATGT 1 cut(s) 43
PctI GAATGC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 111
PkrI GCNGC 2 cut(s) 82, 259
PpuMI RGGWCCY 1 cut(s) 118
PscI ACATGT 1 cut(s) 43
Psp5II RGGWCCY 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 329
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 118
PspPPI RGGWCCY 1 cut(s) 118
PsuI RGATCY 2 cut(s) 333, 350
PvuII CAGCTG 1 cut(s) 400
RsaI GTAC 2 cut(s) 26, 426
RsaNI GTAC 2 cut(s) 25, 425
SaqAI TTAA 3 cut(s) 69, 267, 613
SatI GCNGC 2 cut(s) 81, 258
Sau3AI GATC 2 cut(s) 333, 350
Sau96I GGNCC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 511
SduI GDGCHC 1 cut(s) 163
SfaNI GCATC 4 cut(s) 92, 166, 199, 271
SinI GGWCC 1 cut(s) 118
SmlI CTYRAG 3 cut(s) 354, 539, 596
SmoI CTYRAG 3 cut(s) 354, 539, 596
Sse9I AATT 1 cut(s) 66
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 509
StyI CCWWGG 2 cut(s) 409, 562
TasI AATT 1 cut(s) 66
TatI WGTACW 1 cut(s) 24
Tru1I TTAA 3 cut(s) 69, 267, 613
Tru9I TTAA 3 cut(s) 69, 267, 613
TscAI CASTG 1 cut(s) 352
TseI GCWGC 2 cut(s) 80, 257
TspDTI ATGAA 1 cut(s) 213
TspRI CASTG 1 cut(s) 352
Van91I CCANNNNNTGG 1 cut(s) 111
VneI GTGCAC 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 118
XceI RCATGY 2 cut(s) 47, 457
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.