FvH4_3g21570

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016790
Physical Location & Seq
Reverse (-)
14668259 .. 14669284
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21570.t1

Sequence Viewer

Length: 1026 bp
ATGTATGAAGATCAAGCAATCGAGGATATAAAGCTTGCCCTTCAGCAAATTTCCCTTCAAGAACAATATGAAAAAGGGCAAAGTCATGCAATTCAGGAAGTTGCTATATCTGCAAGACAACCAAGGTTGGTTTGCGAAGTTTGTACTAATACTTTTACCAACAGCTTGGATCGTCTCACTCATTTCCCAGATGGAACTCCAGTTTGCTGTGACGTAATATGGAACCAAACATTTTGTCCCAAACATCTACATGATGGAACTCCAATATGCTTTGGCTGCATGAGGTTAAAGACGAGTGATGTAACATATATAAATCTCGGCGATGGTCGGGAAACATGTCTGGATTGTTATTCTACCGCAATCTTCGACATCCGGCAATGCGAACCTCTCTTGGTCAATAAATGTCACGAATTTTTCCATACACTCGACATTGACCTACCGGACGACGTTCGTGTTTTCTTGGTCGACACGATAGAGATGGCCAGACTTTCTGGCGAGCAAGAACCAATTTTCGGGTTGGGATTAGAGGCTGATATGGATGATATGGCCACCATCACTGGTGTTACAAGTTGTTCGAGGAAAGGGAATAGCATTGTAGTGGAGCAAGAGATGATAGAGGCAAGGGAGTATAGAACCGGCATAGCTATCTTGTTCGGTTTACCCAAGATTATGACACAGGCAATCTTGGCTCATGAGATGATGCATGTATGGTTTCGATCGAAAGGTGTCGAACCCGAGAAACTGGAAAGGAAACTGGAAGAAGGAATGTGTCAAGTAATAGGTTGGAAATGGTTGGATTGGCTGGAGAGTGAAATTAACTCATCATCATCAACAACTACTACTAGTCATGAGCAGACTCAGTTTCGGAGGAAGTTAATAGAAACATACAAGTTTGTGGTGGAAGAACATGACTCTGATGAATATGGCCAAGGATTTAGAGAGGTTGAATCGGATGTTGCAAGATTTGGCCTTAAAACTACAATTAACTGTATGATTAACTCAGCCAACATCGATATCAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

342

Amino Acids

38.93

Weight (kDa)

4.79

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 188 - 330 3.4e-34 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 465
AciI CCGC 1 cut(s) 357
AclWI GGATC 1 cut(s) 177
AcoI YGGCCR 3 cut(s) 480, 546, 925
AcsI RAATTY 2 cut(s) 48, 410
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 512
AflIII ACRYGT 1 cut(s) 335
AgsI TTSAA 2 cut(s) 59, 947
AhlI ACTAGT 1 cut(s) 842
AluBI AGCT 3 cut(s) 34, 165, 644
AluI AGCT 3 cut(s) 34, 165, 644
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 177
Ama87I CYCGRG 1 cut(s) 734
AoxI GGCC 4 cut(s) 480, 546, 925, 967
ApeKI GCWGC 1 cut(s) 276
ApoI RAATTY 2 cut(s) 48, 410
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
AvaI CYCGRG 1 cut(s) 734
BalI TGGCCA 3 cut(s) 482, 548, 927
BbvI GCAGC 1 cut(s) 263
BccI CCATC 5 cut(s) 185, 248, 317, 472, 560
BcoDI GTCTC 1 cut(s) 179
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 1 cut(s) 843
BisI GCNGC 1 cut(s) 277
BlsI GCNGC 1 cut(s) 278
BmeT110I CYCGRG 1 cut(s) 734
BmiI GGNNCC 1 cut(s) 224
BmsI GCATC 1 cut(s) 690
BpmI CTGGAG 2 cut(s) 183, 824
Bsa29I ATCGAT 1 cut(s) 1011
BsaJI CCNNGG 2 cut(s) 122, 928
BsaWI WCCGGW 1 cut(s) 439
Bsc4I CCNNNNNNNGG 1 cut(s) 512
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 4 cut(s) 200, 562, 747, 759
Bse3DI GCAATG 1 cut(s) 383
BseCI ATCGAT 1 cut(s) 1011
BseDI CCNNGG 2 cut(s) 122, 928
BseGI GGATG 3 cut(s) 369, 544, 958
BseLI CCNNNNNNNGG 1 cut(s) 512
BseMI GCAATG 1 cut(s) 383
BseMII CTCAG 2 cut(s) 872, 1014
BseNI ACTGG 4 cut(s) 200, 562, 747, 759
BseXI GCAGC 1 cut(s) 263
Bsh1285I CGRYCG 1 cut(s) 719
BshFI GGCC 4 cut(s) 482, 548, 927, 969
BshVI ATCGAT 1 cut(s) 1011
BsiEI CGRYCG 1 cut(s) 719
BsiHKCI CYCGRG 1 cut(s) 734
BsiSI CCGG 3 cut(s) 373, 440, 636
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 4 cut(s) 482, 548, 927, 969
BsoBI CYCGRG 1 cut(s) 734
Bsp143I GATC 3 cut(s) 10, 169, 716
BspACI CCGC 1 cut(s) 357
BspANI GGCC 4 cut(s) 482, 548, 927, 969
BspCNI CTCAG 2 cut(s) 871, 1013
BspDI ATCGAT 1 cut(s) 1011
BspHI TCATGA 2 cut(s) 691, 847
BspLI GGNNCC 1 cut(s) 224
BspPI GGATC 1 cut(s) 177
BsrDI GCAATG 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 4 cut(s) 200, 562, 747, 759
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 2 cut(s) 122, 928
BssMI GATC 3 cut(s) 10, 169, 716
BssT1I CCWWGG 2 cut(s) 122, 928
Bst4CI ACNGT 1 cut(s) 989
BstC8I GCNNGC 2 cut(s) 36, 497
BstDEI CTNAG 2 cut(s) 858, 1000
BstF5I GGATG 3 cut(s) 369, 544, 958
BstKTI GATC 3 cut(s) 13, 172, 719
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 10, 169, 716
BstMCI CGRYCG 1 cut(s) 719
BstMWI GCNNNNNNNGC 3 cut(s) 110, 276, 686
BstNSI RCATGY 2 cut(s) 339, 707
BstV1I GCAGC 1 cut(s) 263
BstXI CCANNNNNNTGG 1 cut(s) 166
Bsu15I ATCGAT 1 cut(s) 1011
BsuRI GGCC 4 cut(s) 482, 548, 927, 969
BsuTUI ATCGAT 1 cut(s) 1011
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 3 cut(s) 369, 544, 958
BtsIMutI CAGTG 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 36, 497
CciI TCATGA 2 cut(s) 691, 847
Cfr10I RCCGGY 1 cut(s) 635
ClaI ATCGAT 1 cut(s) 1011
Csp6I GTAC 1 cut(s) 144
CviAII CATG 8 cut(s) 86, 251, 280, 336, 692, 704, 848, 908
CviQI GTAC 1 cut(s) 144
DdeI CTNAG 2 cut(s) 858, 1000
DpnI GATC 3 cut(s) 12, 171, 718
DpnII GATC 3 cut(s) 10, 169, 716
EaeI YGGCCR 3 cut(s) 480, 546, 925
Eco130I CCWWGG 2 cut(s) 122, 928
Eco32I GATATC 1 cut(s) 1015
Eco57I CTGAAG 1 cut(s) 26
Eco88I CYCGRG 1 cut(s) 734
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 2 cut(s) 122, 928
EcoT22I ATGCAT 1 cut(s) 705
ErhI CCWWGG 2 cut(s) 122, 928
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 8 cut(s) 89, 254, 283, 339, 695, 707, 851, 911
FaqI GGGAC 1 cut(s) 222
FatI CATG 8 cut(s) 85, 250, 279, 335, 691, 703, 847, 907
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 1 cut(s) 277
FokI GGATG 3 cut(s) 356, 551, 965
Fsp4HI GCNGC 1 cut(s) 277
FspBI CTAG 1 cut(s) 843
GluI GCNGC 1 cut(s) 277
GsuI CTGGAG 2 cut(s) 183, 824
HaeIII GGCC 4 cut(s) 482, 548, 927, 969
HapII CCGG 3 cut(s) 373, 440, 636
Hin1II CATG 8 cut(s) 89, 254, 283, 339, 695, 707, 851, 911
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 3 cut(s) 856, 911, 947
HpaII CCGG 3 cut(s) 373, 440, 636
Hpy166II GTNNAC 2 cut(s) 466, 659
Hpy188I TCNGA 3 cut(s) 867, 916, 952
Hpy188III TCNNGA 7 cut(s) 59, 95, 329, 341, 407, 692, 848
Hpy8I GTNNAC 2 cut(s) 466, 659
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 3 cut(s) 50, 65, 755
HpyCH4III ACNGT 1 cut(s) 989
HpyCH4IV ACGT 2 cut(s) 213, 447
HpyCH4V TGCA 5 cut(s) 89, 113, 279, 703, 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 276, 686
HpyF3I CTNAG 2 cut(s) 858, 1000
HpySE526I ACGT 2 cut(s) 213, 447
Hsp92II CATG 8 cut(s) 89, 254, 283, 339, 695, 707, 851, 911
Kzo9I GATC 3 cut(s) 10, 169, 716
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 1 cut(s) 263
LweI GCATC 1 cut(s) 690
MaeI CTAG 1 cut(s) 843
MaeII ACGT 2 cut(s) 213, 447
MaeIII GTNAC 4 cut(s) 209, 301, 404, 562
MalI GATC 3 cut(s) 12, 171, 718
MboI GATC 3 cut(s) 10, 169, 716
MboII GAAGA 4 cut(s) 20, 355, 770, 914
MlsI TGGCCA 3 cut(s) 482, 548, 927
MluCI AATT 6 cut(s) 48, 90, 410, 507, 813, 981
MluNI TGGCCA 3 cut(s) 482, 548, 927
MlyI GAGTC 2 cut(s) 850, 905
MmeI TCCRAC 2 cut(s) 764, 774
MnlI CCTC 8 cut(s) 16, 276, 396, 520, 570, 610, 861, 934
Mox20I TGGCCA 3 cut(s) 482, 548, 927
Mph1103I ATGCAT 1 cut(s) 705
MscI TGGCCA 3 cut(s) 482, 548, 927
MseI TTAA 6 cut(s) 287, 816, 875, 972, 984, 996
MslI CAYNNNNRTG 2 cut(s) 249, 596
Msp20I TGGCCA 3 cut(s) 482, 548, 927
MspI CCGG 3 cut(s) 373, 440, 636
MwoI GCNNNNNNNGC 3 cut(s) 110, 276, 686
NdeII GATC 3 cut(s) 10, 169, 716
NlaIII CATG 8 cut(s) 89, 254, 283, 339, 695, 707, 851, 911
NlaIV GGNNCC 1 cut(s) 224
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 2 cut(s) 209, 404
NsiI ATGCAT 1 cut(s) 705
NspI RCATGY 2 cut(s) 339, 707
PagI TCATGA 2 cut(s) 691, 847
PciI ACATGT 1 cut(s) 335
PfeI GAWTC 1 cut(s) 947
PkrI GCNGC 1 cut(s) 278
Ple19I CGATCG 1 cut(s) 719
PleI GAGTC 2 cut(s) 850, 905
PpsI GAGTC 2 cut(s) 850, 905
PscI ACATGT 1 cut(s) 335
PspN4I GGNNCC 1 cut(s) 224
PvuI CGATCG 1 cut(s) 719
RsaI GTAC 1 cut(s) 145
RsaNI GTAC 1 cut(s) 144
RseI CAYNNNNRTG 2 cut(s) 249, 596
SalI GTCGAC 1 cut(s) 464
SaqAI TTAA 6 cut(s) 287, 816, 875, 972, 984, 996
SatI GCNGC 1 cut(s) 277
Sau3AI GATC 3 cut(s) 10, 169, 716
SchI GAGTC 2 cut(s) 850, 905
SfaNI GCATC 1 cut(s) 690
SmiMI CAYNNNNRTG 2 cut(s) 249, 596
SpeI ACTAGT 1 cut(s) 842
Sse9I AATT 6 cut(s) 48, 90, 410, 507, 813, 981
SsiI CCGC 1 cut(s) 357
SspMI CTAG 1 cut(s) 843
StyI CCWWGG 2 cut(s) 122, 928
TaaI ACNGT 1 cut(s) 989
TaiI ACGT 2 cut(s) 216, 450
TaqI TCGA 9 cut(s) 21, 366, 426, 465, 575, 715, 719, 729, 1011
TasI AATT 6 cut(s) 48, 90, 410, 507, 813, 981
TatI WGTACW 1 cut(s) 143
TfiI GAWTC 1 cut(s) 947
Tru1I TTAA 6 cut(s) 287, 816, 875, 972, 984, 996
Tru9I TTAA 6 cut(s) 287, 816, 875, 972, 984, 996
TscAI CASTG 1 cut(s) 562
TseFI GTSAC 2 cut(s) 209, 404
TseI GCWGC 1 cut(s) 276
Tsp45I GTSAC 2 cut(s) 209, 404
TspDTI ATGAA 3 cut(s) 21, 84, 933
TspRI CASTG 1 cut(s) 562
XapI RAATTY 2 cut(s) 48, 410
XceI RCATGY 2 cut(s) 339, 707
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 1 cut(s) 843
Zsp2I ATGCAT 1 cut(s) 705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.