Rmu_sc0008296.1_g000003

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008296.1
OG0016790
Physical Location & Seq
Reverse (-)
15573 .. 16604
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008296.1_g000003.1.cds

Sequence Viewer

Length: 1032 bp
atgtatgaagatcaagatatcgacaatatagagcttgccatgcagctaatttccgtttcagatcatcaatatgaaaaagggcaaagtgatgcaaataaggaagttgctatatcttcaagacaaccaatgttggtttgcgatgtttgtactaatacaattatcgagagcttgcatcatcccacttatttcccagatggagctccagtttgctgcgacataatatggaaacaaacaatttgtcccaaacatctagaggatagaactccaatatgctgtggctgcatgaggttaaggacaagtgatgtaacatatataaatctcggtgaaggtcgggaaatatgcccagattgttattctaccgcaatcgtcgatatccgacaatgtgaacctctcatgatccataaatgtcacgaatttttccgtacactcaacctggaccttccggacgacgttcgtgtcttcttggtcgacaagaaagagatggatagactaaactgcgagcaagaacccacgcctttcgggttgcgtttcaaggcagatatgggaaccatcaccggcgttacaagttgttcgcggaaaggggatagcgttgtagtggagcgacagtttcatggtgattataaaaccggcatagcaattttgttcggtttgcccaagattatgacgcaggcaatcttggctcatgagatgatgcatgtatggtttcgatcgaaaggtatcatattcggggaactggaaaagaaactggaagaaggaatgtgtcaagtaataggttggaaatggttagattggctggacagtgaaattacttcatcatcatcagcaactactagtcatgagcaggctcagtttctgagaaaattagtacaaacatacaagtttgtggtggaaaagcatgactctgatgaatatggccaaggatttagagaggtccaacgggcagttgcaagatttggccttgaaactacaattaatcatatgattacaacttacaaggaaaaagcttccagaagaatgccagtcacactctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

343

Amino Acids

39.34

Weight (kDa)

5.58

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AasI GACNNNNNNGTC 1 cut(s) 455
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 574
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 2 cut(s) 360, 574
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 1 cut(s) 913
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 3 cut(s) 148, 424, 867
AgsI TTSAA 3 cut(s) 117, 532, 962
AhlI ACTAGT 1 cut(s) 830
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 5 cut(s) 34, 46, 168, 200, 1004
AluI AGCT 5 cut(s) 34, 46, 168, 200, 1004
Alw21I GWGCWC 1 cut(s) 202
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 1 cut(s) 853
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 2 cut(s) 913, 955
ApeKI GCWGC 3 cut(s) 43, 210, 279
ApoI RAATTY 1 cut(s) 413
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AseI ATTAAT 1 cut(s) 972
AspS9I GGNCC 2 cut(s) 436, 931
AsuHPI GGTGA 3 cut(s) 335, 544, 626
AvaII GGWCC 2 cut(s) 436, 931
BalI TGGCCA 1 cut(s) 915
BanII GRGCYC 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 3 cut(s) 55, 197, 266
BccI CCATC 3 cut(s) 188, 475, 557
BciT130I CCWGG 1 cut(s) 434
BcuI ACTAGT 1 cut(s) 830
BfaI CTAG 3 cut(s) 251, 831, 1030
BisI GCNGC 3 cut(s) 44, 211, 280
BlsI GCNGC 3 cut(s) 45, 212, 281
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 2 cut(s) 436, 931
BmgT120I GGNCC 2 cut(s) 436, 931
BmiI GGNNCC 1 cut(s) 547
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 3 cut(s) 79, 181, 681
BpiI GAAGAC 1 cut(s) 451
BpmI CTGGAG 1 cut(s) 186
BsaJI CCNNGG 1 cut(s) 916
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 590, 620
Bse118I RCCGGY 2 cut(s) 554, 626
Bse1I ACTGG 4 cut(s) 203, 738, 750, 1019
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 916
BseGI GGATG 1 cut(s) 175
BseMII CTCAG 2 cut(s) 845, 860
BseNI ACTGG 4 cut(s) 203, 738, 750, 1019
BseXI GCAGC 3 cut(s) 55, 197, 266
Bsh1236I CGCG 1 cut(s) 574
Bsh1285I CGRYCG 1 cut(s) 710
BshFI GGCC 2 cut(s) 915, 957
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 202
BsiSI CCGG 3 cut(s) 443, 555, 627
BslFI GGGAC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 225
BsmI GAATGC 1 cut(s) 1020
BsnI GGCC 2 cut(s) 915, 957
Bsp1286I GDGCHC 1 cut(s) 202
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 4 cut(s) 10, 61, 396, 707
BspACI CCGC 2 cut(s) 360, 574
BspANI GGCC 2 cut(s) 915, 957
BspCNI CTCAG 2 cut(s) 846, 859
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 1 cut(s) 574
BspHI TCATGA 3 cut(s) 393, 682, 835
BspLI GGNNCC 1 cut(s) 547
BspPI GGATC 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 554, 626
BsrI ACTGG 4 cut(s) 203, 738, 750, 1019
BssAI RCCGGY 2 cut(s) 554, 626
BssECI CCNNGG 1 cut(s) 916
BssMI GATC 4 cut(s) 10, 61, 396, 707
BssT1I CCWWGG 1 cut(s) 916
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 2 cut(s) 606, 800
BstC8I GCNNGC 5 cut(s) 36, 170, 500, 669, 843
BstDEI CTNAG 2 cut(s) 846, 854
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 4 cut(s) 13, 64, 399, 710
BstMBI GATC 4 cut(s) 10, 61, 396, 707
BstMCI CGRYCG 1 cut(s) 710
BstMWI GCNNNNNNNGC 3 cut(s) 40, 279, 677
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 698
BstSCI CCNGG 1 cut(s) 432
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 55, 197, 266
BstV2I GAAGAC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 915, 957
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 805
Cac8I GCNNGC 5 cut(s) 36, 170, 500, 669, 843
CaiI CAGNNNCTG 1 cut(s) 853
CciI TCATGA 3 cut(s) 393, 682, 835
Cfr10I RCCGGY 2 cut(s) 554, 626
Cfr13I GGNCC 2 cut(s) 436, 931
CseI GACGC 1 cut(s) 673
Csp6I GTAC 3 cut(s) 147, 423, 866
CviAII CATG 8 cut(s) 40, 283, 394, 611, 683, 695, 836, 896
CviQI GTAC 3 cut(s) 147, 423, 866
DdeI CTNAG 2 cut(s) 846, 854
DpnI GATC 4 cut(s) 12, 63, 398, 709
DpnII GATC 4 cut(s) 10, 61, 396, 707
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 1 cut(s) 913
Ecl136II GAGCTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 916
Eco24I GRGCYC 1 cut(s) 202
Eco32I GATATC 2 cut(s) 19, 373
Eco47I GGWCC 2 cut(s) 436, 931
Eco53kI GAGCTC 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 432
EcoRV GATATC 2 cut(s) 19, 373
EcoT14I CCWWGG 1 cut(s) 916
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 202
ErhI CCWWGG 1 cut(s) 916
FaeI CATG 8 cut(s) 43, 286, 397, 614, 686, 698, 839, 899
FaqI GGGAC 1 cut(s) 225
FatI CATG 8 cut(s) 39, 282, 393, 610, 682, 694, 835, 895
FauNDI CATATG 1 cut(s) 978
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 44, 211, 280
FokI GGATG 1 cut(s) 162
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 44, 211, 280
FspBI CTAG 3 cut(s) 251, 831, 1030
GluI GCNGC 3 cut(s) 44, 211, 280
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 915, 957
HapII CCGG 3 cut(s) 443, 555, 627
HgaI GACGC 1 cut(s) 673
Hin1II CATG 8 cut(s) 43, 286, 397, 614, 686, 698, 839, 899
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 1 cut(s) 899
HpaII CCGG 3 cut(s) 443, 555, 627
HphI GGTGA 3 cut(s) 335, 544, 626
Hpy166II GTNNAC 3 cut(s) 386, 425, 469
Hpy188I TCNGA 4 cut(s) 61, 377, 855, 904
Hpy8I GTNNAC 3 cut(s) 386, 425, 469
Hpy99I CGWCG 2 cut(s) 371, 452
HpyAV CCTTC 3 cut(s) 320, 449, 746
HpyCH4III ACNGT 2 cut(s) 606, 800
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 6 cut(s) 43, 92, 172, 282, 694, 947
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 279, 677
HpyF3I CTNAG 2 cut(s) 846, 854
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 8 cut(s) 43, 286, 397, 614, 686, 698, 839, 899
Kpn2I TCCGGA 1 cut(s) 442
Kzo9I GATC 4 cut(s) 10, 61, 396, 707
LmnI GCTCC 3 cut(s) 197, 205, 598
Lsp1109I GCAGC 3 cut(s) 55, 197, 266
LweI GCATC 3 cut(s) 79, 181, 681
MaeI CTAG 3 cut(s) 251, 831, 1030
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 4 cut(s) 304, 407, 559, 1021
MalI GATC 4 cut(s) 12, 63, 398, 709
MboI GATC 4 cut(s) 10, 61, 396, 707
MboII GAAGA 5 cut(s) 20, 105, 451, 761, 1023
MhlI GDGCHC 1 cut(s) 202
MlsI TGGCCA 1 cut(s) 915
MluCI AATT 8 cut(s) 48, 156, 234, 413, 636, 804, 860, 969
MluNI TGGCCA 1 cut(s) 915
MlyI GAGTC 1 cut(s) 893
MmeI TCCRAC 3 cut(s) 400, 755, 958
MnlI CCTC 4 cut(s) 247, 279, 399, 922
Mox20I TGGCCA 1 cut(s) 915
Mph1103I ATGCAT 1 cut(s) 696
MroI TCCGGA 1 cut(s) 442
MscI TGGCCA 1 cut(s) 915
MseI TTAA 2 cut(s) 290, 972
MslI CAYNNNNRTG 1 cut(s) 69
Msp20I TGGCCA 1 cut(s) 915
MspI CCGG 3 cut(s) 443, 555, 627
MspR9I CCNGG 1 cut(s) 434
Mva1269I GAATGC 1 cut(s) 1020
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 3 cut(s) 40, 279, 677
NdeI CATATG 1 cut(s) 978
NdeII GATC 4 cut(s) 10, 61, 396, 707
NlaIII CATG 8 cut(s) 43, 286, 397, 614, 686, 698, 839, 899
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 2 cut(s) 407, 1021
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 1 cut(s) 698
PagI TCATGA 3 cut(s) 393, 682, 835
PctI GAATGC 1 cut(s) 1020
PkrI GCNGC 3 cut(s) 45, 212, 281
Ple19I CGATCG 1 cut(s) 710
PleI GAGTC 1 cut(s) 893
PpsI GAGTC 1 cut(s) 893
PshBI ATTAAT 1 cut(s) 972
PsiI TTATAA 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 547
PspPI GGNCC 2 cut(s) 436, 931
PstNI CAGNNNCTG 1 cut(s) 853
PvuI CGATCG 1 cut(s) 710
RsaI GTAC 3 cut(s) 148, 424, 867
RsaNI GTAC 3 cut(s) 147, 423, 866
RseI CAYNNNNRTG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 467
SaqAI TTAA 2 cut(s) 290, 972
SatI GCNGC 3 cut(s) 44, 211, 280
Sau3AI GATC 4 cut(s) 10, 61, 396, 707
Sau96I GGNCC 2 cut(s) 436, 931
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 3 cut(s) 79, 181, 681
SgrAI CRCCGGYG 1 cut(s) 554
SinI GGWCC 2 cut(s) 436, 931
SmiMI CAYNNNNRTG 1 cut(s) 69
SpeI ACTAGT 1 cut(s) 830
Sse9I AATT 8 cut(s) 48, 156, 234, 413, 636, 804, 860, 969
SsiI CCGC 2 cut(s) 360, 574
SspMI CTAG 3 cut(s) 251, 831, 1030
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 432
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 2 cut(s) 606, 800
TaiI ACGT 1 cut(s) 453
TaqI TCGA 6 cut(s) 21, 162, 369, 468, 706, 710
TasI AATT 8 cut(s) 48, 156, 234, 413, 636, 804, 860, 969
TatI WGTACW 2 cut(s) 146, 865
Tru1I TTAA 2 cut(s) 290, 972
Tru9I TTAA 2 cut(s) 290, 972
TscAI CASTG 1 cut(s) 805
TseFI GTSAC 2 cut(s) 407, 1021
TseI GCWGC 3 cut(s) 43, 210, 279
Tsp45I GTSAC 2 cut(s) 407, 1021
TspDTI ATGAA 5 cut(s) 21, 87, 599, 801, 921
TspGWI ACGGA 2 cut(s) 43, 410
TspRI CASTG 1 cut(s) 805
VpaK11BI GGWCC 2 cut(s) 436, 931
VspI ATTAAT 1 cut(s) 972
XapI RAATTY 1 cut(s) 413
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 3 cut(s) 251, 831, 1030
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.