Rw5G023210

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016790
Physical Location & Seq
Reverse (-)
32688728 .. 32689291
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023210.1

Sequence Viewer

Length: 564 bp
ATGGATAGACTAAACTGCGAGCAAGAACCCACGCCTTTCGGGTTGCCTTTCAAGGCAGATATGGGAACCATCACCGGCGTTACAAGTTGTTCGCGGAAAGGGGATAGCGTTGTAGTGGAGCGACAGTTTCATGGTGATTATAAAACCGGCATAGCAATTTTGTTCGGTTTGCCCAAGATTATGACGCAGGCAATCTTGGCTCATGAGATGATGCATGTATGGTTTCGATCGAAAGGTATCATATTCGGGGAACTGGAAAAGAAACTGGAAGAAGGAATGTGTCAAGTAATAGGTTGGAAATGGTTGGATTGGCTGGACAGTGAAATTACTTCATCATCATCAGCAACTACTAGTCATGAGCAGGCTCAGTTTCTGAGAAAATTAGTACAAACATACAAGTTTGTGGTGGAAAAGCATGACTCTGATGAATATGGCCAAGGATTTAGAGAGGTCCAACGGGCAGTTGCAAGATTTGGCCTTGAAACTACAATTAATCATATGATTACAACTTACAAGGAAAAAGCTTCCAGAAGAATGCCAGTCACACTCCAGCTAGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

187

Amino Acids

21.35

Weight (kDa)

7.01

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 48 - 169 2.3e-35 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 141
AccII CGCG 1 cut(s) 94
AciI CCGC 1 cut(s) 94
AcoI YGGCCR 1 cut(s) 433
AfaI GTAC 1 cut(s) 387
AgsI TTSAA 2 cut(s) 52, 482
AhlI ACTAGT 1 cut(s) 350
AluBI AGCT 3 cut(s) 524, 553, 557
AluI AGCT 3 cut(s) 524, 553, 557
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 2 cut(s) 433, 475
AseI ATTAAT 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 451
AsuHPI GGTGA 2 cut(s) 64, 146
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 1 cut(s) 451
BalI TGGCCA 1 cut(s) 435
BccI CCATC 1 cut(s) 77
BcuI ACTAGT 1 cut(s) 350
BfaI CTAG 3 cut(s) 351, 554, 562
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 1 cut(s) 451
BmiI GGNNCC 1 cut(s) 67
BmsI GCATC 1 cut(s) 201
BmtI GCTAGC 1 cut(s) 557
BpmI CTGGAG 1 cut(s) 533
BsaJI CCNNGG 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bse118I RCCGGY 2 cut(s) 74, 146
Bse1I ACTGG 3 cut(s) 258, 270, 539
BseDI CCNNGG 1 cut(s) 436
BseMII CTCAG 2 cut(s) 365, 380
BseNI ACTGG 3 cut(s) 258, 270, 539
Bsh1236I CGCG 1 cut(s) 94
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 2 cut(s) 435, 477
BsiEI CGRYCG 1 cut(s) 230
BsiSI CCGG 2 cut(s) 75, 147
BsmI GAATGC 1 cut(s) 540
BsnI GGCC 2 cut(s) 435, 477
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 1 cut(s) 94
BspANI GGCC 2 cut(s) 435, 477
BspCNI CTCAG 2 cut(s) 366, 379
BspFNI CGCG 1 cut(s) 94
BspHI TCATGA 2 cut(s) 202, 355
BspLI GGNNCC 1 cut(s) 67
BspOI GCTAGC 1 cut(s) 557
BsrFI RCCGGY 2 cut(s) 74, 146
BsrI ACTGG 3 cut(s) 258, 270, 539
BssAI RCCGGY 2 cut(s) 74, 146
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 1 cut(s) 227
BssT1I CCWWGG 1 cut(s) 436
Bst4CI ACNGT 2 cut(s) 126, 320
BstC8I GCNNGC 4 cut(s) 20, 189, 363, 555
BstDEI CTNAG 2 cut(s) 366, 374
BstFNI CGCG 1 cut(s) 94
BstKTI GATC 1 cut(s) 230
BstMBI GATC 1 cut(s) 227
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNSI RCATGY 1 cut(s) 218
BstUI CGCG 1 cut(s) 94
BsuRI GGCC 2 cut(s) 435, 477
BtsIMutI CAGTG 1 cut(s) 325
Cac8I GCNNGC 4 cut(s) 20, 189, 363, 555
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 2 cut(s) 202, 355
Cfr10I RCCGGY 2 cut(s) 74, 146
Cfr13I GGNCC 1 cut(s) 451
CseI GACGC 1 cut(s) 193
Csp6I GTAC 1 cut(s) 386
CviAII CATG 5 cut(s) 131, 203, 215, 356, 416
CviJI RGCY 8 cut(s) 200, 313, 365, 435, 477, 524, 553, 557
CviKI_1 RGCY 8 cut(s) 200, 313, 365, 435, 477, 524, 553, 557
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 2 cut(s) 366, 374
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
EaeI YGGCCR 1 cut(s) 433
Eco130I CCWWGG 1 cut(s) 436
Eco47I GGWCC 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 5 cut(s) 134, 206, 218, 359, 419
FatI CATG 5 cut(s) 130, 202, 214, 355, 415
FauNDI CATATG 1 cut(s) 498
FspBI CTAG 3 cut(s) 351, 554, 562
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 2 cut(s) 435, 477
HapII CCGG 2 cut(s) 75, 147
HgaI GACGC 1 cut(s) 193
Hin1II CATG 5 cut(s) 134, 206, 218, 359, 419
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 1 cut(s) 419
HpaII CCGG 2 cut(s) 75, 147
HphI GGTGA 2 cut(s) 64, 146
Hpy188I TCNGA 2 cut(s) 375, 424
Hpy188III TCNNGA 3 cut(s) 203, 356, 528
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 2 cut(s) 126, 320
HpyCH4V TGCA 2 cut(s) 214, 467
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 2 cut(s) 366, 374
Hsp92II CATG 5 cut(s) 134, 206, 218, 359, 419
Kzo9I GATC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 118
LpnPI CCDG 9 cut(s) 88, 160, 173, 239, 251, 299, 347, 541, 552
LweI GCATC 1 cut(s) 201
MaeI CTAG 3 cut(s) 351, 554, 562
MaeIII GTNAC 2 cut(s) 79, 541
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MboII GAAGA 2 cut(s) 281, 543
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 4 cut(s) 156, 324, 380, 489
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 3 cut(s) 275, 285, 478
MnlI CCTC 1 cut(s) 442
Mox20I TGGCCA 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 216
MscI TGGCCA 1 cut(s) 435
MseI TTAA 1 cut(s) 492
Msp20I TGGCCA 1 cut(s) 435
MspI CCGG 2 cut(s) 75, 147
Mva1269I GAATGC 1 cut(s) 540
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeI CATATG 1 cut(s) 498
NdeII GATC 1 cut(s) 227
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 5 cut(s) 134, 206, 218, 359, 419
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 541
NsiI ATGCAT 1 cut(s) 216
NspI RCATGY 1 cut(s) 218
PagI TCATGA 2 cut(s) 202, 355
PctI GAATGC 1 cut(s) 540
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
PshBI ATTAAT 1 cut(s) 492
PsiI TTATAA 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 1 cut(s) 451
PstNI CAGNNNCTG 1 cut(s) 373
PvuI CGATCG 1 cut(s) 230
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
SaqAI TTAA 1 cut(s) 492
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 1 cut(s) 451
SchI GAGTC 1 cut(s) 413
SetI ASST 6 cut(s) 238, 295, 453, 526, 555, 559
SfaNI GCATC 1 cut(s) 201
SgrAI CRCCGGYG 1 cut(s) 74
SinI GGWCC 1 cut(s) 451
SpeI ACTAGT 1 cut(s) 350
Sse9I AATT 4 cut(s) 156, 324, 380, 489
SsiI CCGC 1 cut(s) 94
SspMI CTAG 3 cut(s) 351, 554, 562
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 126, 320
TaqI TCGA 2 cut(s) 226, 230
TasI AATT 4 cut(s) 156, 324, 380, 489
TatI WGTACW 1 cut(s) 385
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 325
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 3 cut(s) 119, 321, 441
TspRI CASTG 1 cut(s) 325
VpaK11BI GGWCC 1 cut(s) 451
VspI ATTAAT 1 cut(s) 492
XceI RCATGY 1 cut(s) 218
XspI CTAG 3 cut(s) 351, 554, 562
Zsp2I ATGCAT 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.