RLG00000024635

actinin binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0016790
Physical Location & Seq
Forward (+)
37652853 .. 37654296
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024635

Sequence Viewer

Length: 642 bp
ATGGGTGGTGCTTTGGCACATGAAATGATGCATACGTGGTTGGAGCTTGAAGTGGGCGGTGGGATTTTGGAAAAAAGAGTTGAAGAAGGTATTTGTGAAGTAATGAATTACATGTGGATTGAATGGTTTAGTGCTAATGAAGGCGTTGAGTACTCCTATAAAACTGACGAGCAAGCTGAGTTTACAAGAAAGTTGAAGGAGTTCTACCAACAGCGAAAGGAACGCGGTGAACACGAATGTTATGGCCAAGGGTTCAGAGACGCCATGTGGGCAATGCAAATATTTGGCCTCGAAACTACACTAGAACATATTGATGGTTCAGAAGGATTTAAAAAACACATCCGTGGCCAAGATTTTGTGTTGAGCAGTTGTATGGAGACTCCTAAGAGAAATTGGATGACTAGGTCAGGTCTTCATCCGACTTCTCTAAGAGAAGCTTCTTCTCGACTGACAAGAGCCTTCCCACAGCATAAGCATGACCGGATGTTGCAGCCTTGCCGGAGCCGCAAACAAGATCTTCCCGTAGTCAGACGAGTGGAAACTTCCATCAACTATCTTCGGAGAATAGTGGAGGGAACGCCAAGGGATACGGTTCCTACTTGGGAAGTGGGAACAAATGCTTCAAACTTTATTTTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000131 GO:0000278 GO:0000281 GO:0000746 GO:0000747 GO:0000755 GO:0000910 GO:0000917 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005933 GO:0005934 GO:0005935 GO:0005937 GO:0006109 GO:0006996 GO:0007010 GO:0007049 GO:0008047 GO:0008150 GO:0008360 GO:0009272 GO:0009889 GO:0009966 GO:0009968 GO:0009987 GO:0010556 GO:0010646 GO:0010648 GO:0010675 GO:0010962 GO:0010981 GO:0016043 GO:0019222 GO:0019904 GO:0019953 GO:0022402 GO:0022413 GO:0022414 GO:0022603 GO:0022604 GO:0022607 GO:0023051 GO:0023057 GO:0030016 GO:0030017 GO:0030029 GO:0030036 GO:0030234 GO:0030427 GO:0030695 GO:0031323 GO:0031326 GO:0031430 GO:0031672 GO:0032505 GO:0032506 GO:0032881 GO:0032885 GO:0032950 GO:0032951 GO:0032952 GO:0032953 GO:0032995 GO:0035023 GO:0035024 GO:0042546 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043255 GO:0043292 GO:0043332 GO:0043547 GO:0044085 GO:0044087 GO:0044093 GO:0044422 GO:0044424 GO:0044444 GO:0044449 GO:0044463 GO:0044464 GO:0044703 GO:0044764 GO:0046578 GO:0046580 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0051056 GO:0051058 GO:0051128 GO:0051179 GO:0051286 GO:0051301 GO:0051336 GO:0051345 GO:0051641 GO:0051666 GO:0051704 GO:0055120 GO:0060237 GO:0060255 GO:0060589 GO:0061640 GO:0065007 GO:0065008 GO:0065009 GO:0071554 GO:0071840 GO:0071852 GO:0080090 GO:0090036 GO:0090038 GO:0090334 GO:0090529 GO:0098772 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120038 GO:1902410 GO:1902531 GO:1902532 GO:1903047 GO:1903338 GO:2000112
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM RhoGAP

Protein Analysis

214

Amino Acids

24.96

Weight (kDa)

6.46

Isoelectric Point (pI)

50.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 2 - 105 3e-24 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 225
AciI CCGC 3 cut(s) 57, 225, 505
AcoI YGGCCR 2 cut(s) 244, 346
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 152
AflIII ACRYGT 1 cut(s) 111
AgsI TTSAA 5 cut(s) 50, 83, 122, 196, 624
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 3 cut(s) 46, 176, 437
AluI AGCT 3 cut(s) 46, 176, 437
Alw26I GTCTC 2 cut(s) 252, 371
AoxI GGCC 3 cut(s) 244, 286, 346
ApeKI GCWGC 1 cut(s) 490
Asp700I GAANNNNTTC 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 239
BalI TGGCCA 2 cut(s) 246, 348
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbsI GAAGAC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 502
BccI CCATC 2 cut(s) 308, 554
BciVI GTATCC 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 252, 371
BfaI CTAG 2 cut(s) 302, 402
BfuI GTATCC 1 cut(s) 580
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 1 cut(s) 514
BisI GCNGC 2 cut(s) 491, 505
BlsI GCNGC 2 cut(s) 492, 506
BmcAI AGTACT 1 cut(s) 152
BmiI GGNNCC 2 cut(s) 503, 594
BmsI GCATC 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 404
BsaAI YACGTR 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 3 cut(s) 247, 343, 581
BsaWI WCCGGW 1 cut(s) 480
Bse3DI GCAATG 1 cut(s) 279
BseDI CCNNGG 3 cut(s) 247, 343, 581
BseGI GGATG 4 cut(s) 339, 402, 415, 489
BseMI GCAATG 1 cut(s) 279
BseMII CTCAG 1 cut(s) 168
BseXI GCAGC 1 cut(s) 502
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 246, 288, 348
BsiSI CCGG 2 cut(s) 481, 499
BsmAI GTCTC 2 cut(s) 252, 371
BsmBI CGTCTC 1 cut(s) 252
BsnI GGCC 3 cut(s) 246, 288, 348
Bsp143I GATC 1 cut(s) 514
BspACI CCGC 3 cut(s) 57, 225, 505
BspANI GGCC 3 cut(s) 246, 288, 348
BspCNI CTCAG 1 cut(s) 169
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 503, 594
BsrDI GCAATG 1 cut(s) 279
BssECI CCNNGG 3 cut(s) 247, 343, 581
BssMI GATC 1 cut(s) 514
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 2 cut(s) 247, 581
Bst4CI ACNGT 1 cut(s) 592
BstACI GRCGYC 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 3 cut(s) 177, 384, 428
BstDSI CCRYGG 1 cut(s) 343
BstF5I GGATG 4 cut(s) 339, 402, 415, 489
BstFNI CGCG 1 cut(s) 225
BstKTI GATC 1 cut(s) 517
BstMAI GTCTC 2 cut(s) 252, 371
BstMBI GATC 1 cut(s) 514
BstMWI GCNNNNNNNGC 2 cut(s) 269, 504
BstNSI RCATGY 1 cut(s) 115
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 502
BstV2I GAAGAC 1 cut(s) 404
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 3 cut(s) 246, 288, 348
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 4 cut(s) 339, 402, 415, 489
Cac8I GCNNGC 1 cut(s) 174
CseI GACGC 1 cut(s) 269
Csp6I GTAC 1 cut(s) 151
CviAII CATG 4 cut(s) 20, 112, 265, 476
CviJI RGCY 9 cut(s) 46, 176, 246, 288, 348, 437, 458, 493, 504
CviKI_1 RGCY 9 cut(s) 46, 176, 246, 288, 348, 437, 458, 493, 504
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 3 cut(s) 177, 384, 428
DpnI GATC 1 cut(s) 516
DpnII GATC 1 cut(s) 514
DraI TTTAAA 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 244, 346
Eco130I CCWWGG 2 cut(s) 247, 581
EcoT14I CCWWGG 2 cut(s) 247, 581
EcoT22I ATGCAT 1 cut(s) 33
ErhI CCWWGG 2 cut(s) 247, 581
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 4 cut(s) 23, 115, 268, 479
FalI AAGNNNNNCTT 2 cut(s) 421, 453
FatI CATG 4 cut(s) 19, 111, 264, 475
Fnu4HI GCNGC 2 cut(s) 491, 505
FokI GGATG 4 cut(s) 326, 402, 409, 496
Fsp4HI GCNGC 2 cut(s) 491, 505
FspBI CTAG 2 cut(s) 302, 402
GluI GCNGC 2 cut(s) 491, 505
HaeIII GGCC 3 cut(s) 246, 288, 348
HapII CCGG 2 cut(s) 481, 499
HgaI GACGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 4 cut(s) 23, 115, 268, 479
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 1 cut(s) 379
HpaII CCGG 2 cut(s) 481, 499
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 2 cut(s) 183, 230
Hpy188I TCNGA 5 cut(s) 257, 322, 420, 530, 561
Hpy188III TCNNGA 1 cut(s) 444
Hpy8I GTNNAC 2 cut(s) 183, 230
HpyAV CCTTC 5 cut(s) 80, 134, 190, 317, 469
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 3 cut(s) 31, 277, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 269, 504
HpyF3I CTNAG 3 cut(s) 177, 384, 428
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 4 cut(s) 23, 115, 268, 479
Kzo9I GATC 1 cut(s) 514
LmnI GCTCC 2 cut(s) 43, 501
LpnPI CCDG 3 cut(s) 393, 494, 512
Lsp1109I GCAGC 1 cut(s) 502
LweI GCATC 1 cut(s) 18
MaeI CTAG 2 cut(s) 302, 402
MaeII ACGT 1 cut(s) 35
MalI GATC 1 cut(s) 516
MboI GATC 1 cut(s) 514
MboII GAAGA 5 cut(s) 95, 404, 432, 509, 548
MflI RGATCY 1 cut(s) 514
MlsI TGGCCA 2 cut(s) 246, 348
MluCI AATT 2 cut(s) 106, 391
MluNI TGGCCA 2 cut(s) 246, 348
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 2 cut(s) 21, 443
MnlI CCTC 2 cut(s) 299, 565
Mox20I TGGCCA 2 cut(s) 246, 348
Mph1103I ATGCAT 1 cut(s) 33
MroXI GAANNNNTTC 1 cut(s) 200
MscI TGGCCA 2 cut(s) 246, 348
MseI TTAA 1 cut(s) 330
MslI CAYNNNNRTG 3 cut(s) 312, 342, 474
Msp20I TGGCCA 2 cut(s) 246, 348
MspI CCGG 2 cut(s) 481, 499
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 269, 504
NdeII GATC 1 cut(s) 514
NlaIII CATG 4 cut(s) 23, 115, 268, 479
NlaIV GGNNCC 2 cut(s) 503, 594
NsiI ATGCAT 1 cut(s) 33
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 342
PciI ACATGT 1 cut(s) 111
PdmI GAANNNNTTC 1 cut(s) 200
PflFI GACNNNGTC 1 cut(s) 403
PkrI GCNGC 2 cut(s) 492, 506
PleI GAGTC 1 cut(s) 373
PpsI GAGTC 1 cut(s) 373
Ppu21I YACGTR 1 cut(s) 36
PscI ACATGT 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 503, 594
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 1 cut(s) 403
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
RseI CAYNNNNRTG 3 cut(s) 312, 342, 474
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 2 cut(s) 491, 505
Sau3AI GATC 1 cut(s) 514
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 373
SetI ASST 7 cut(s) 38, 48, 91, 178, 407, 412, 439
SfaNI GCATC 1 cut(s) 18
SmiMI CAYNNNNRTG 3 cut(s) 312, 342, 474
Sse9I AATT 2 cut(s) 106, 391
SsiI CCGC 3 cut(s) 57, 225, 505
SspI AATATT 1 cut(s) 282
SspMI CTAG 2 cut(s) 302, 402
StyI CCWWGG 2 cut(s) 247, 581
TaaI ACNGT 1 cut(s) 592
TaiI ACGT 1 cut(s) 38
TaqI TCGA 2 cut(s) 291, 445
TasI AATT 2 cut(s) 106, 391
TatI WGTACW 1 cut(s) 150
TauI GCSGC 1 cut(s) 507
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TseI GCWGC 1 cut(s) 490
TspDTI ATGAA 4 cut(s) 36, 119, 153, 404
TspGWI ACGGA 1 cut(s) 332
Tth111I GACNNNGTC 1 cut(s) 403
XceI RCATGY 1 cut(s) 115
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 2 cut(s) 302, 402
ZrmI AGTACT 1 cut(s) 152
Zsp2I ATGCAT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.