Rmu_co8219504.1_g000001

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8219504.1
OG0016790
Physical Location & Seq
Forward (+)
20 .. 738
719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8219504.1_g000001.1.cds

Sequence Viewer

Length: 719 bp
atgtatgaagatcaagatatcgacaatatagagcttgccatgcagctaatttccgtttcagatcatcaatatgaaaaagggcaaagtgatgcaaataaggaagttgctatatcttcaagacaaccaatgttggtttgcgatgtttgtactaatacaattatcgagagcttgcatcatcccacttatttcccagatggagctccagtttgctgcgacataatatggaaacaaacaatttgtcccaaacatctagaggatagaactccaatatgctgtggctgcatgaggttaaggacaagtgatgtaacatatataaatctcggtgaaggtcgggaaatatgcccagattgttattctaccgcaatcgtcgatatccgacaatgtgaacctctcatgatccataaatgtcacgaatttttccgtacactcaacctggaccttccggacgacgttcgtgtcttcttggtcgacaagaaagagatggatagactaaactgcgagcaagaacccacgcctttcgggttgcgtttcaaggcagatatgggaaccatcaccggcgttacaagttgttcgcggaaaggggatagcgttgtagtggagcgacagtttcatggtgattataaaaccggcatagcaattttgttcggtttgcccaagattatgacgcaggcaatcttggctcatgagatgatgcatgtatggtttcgatcgaaaggtat
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

239

Amino Acids

27.24

Weight (kDa)

5.34

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 621
AasI GACNNNNNNGTC 1 cut(s) 455
AccI GTMKAC 1 cut(s) 468
AccII CGCG 1 cut(s) 574
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 2 cut(s) 360, 574
AclWI GGATC 1 cut(s) 391
AcsI RAATTY 1 cut(s) 413
AfaI GTAC 2 cut(s) 148, 424
AgsI TTSAA 2 cut(s) 117, 532
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 4 cut(s) 34, 46, 168, 200
AluI AGCT 4 cut(s) 34, 46, 168, 200
Alw21I GWGCWC 1 cut(s) 202
AlwI GGATC 1 cut(s) 391
Aor13HI TCCGGA 1 cut(s) 442
ApeKI GCWGC 3 cut(s) 43, 210, 279
ApoI RAATTY 1 cut(s) 413
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AspS9I GGNCC 1 cut(s) 436
AsuHPI GGTGA 3 cut(s) 335, 544, 626
AvaII GGWCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 451
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 3 cut(s) 55, 197, 266
BccI CCATC 3 cut(s) 188, 475, 557
BciT130I CCWGG 1 cut(s) 434
BfaI CTAG 1 cut(s) 251
BisI GCNGC 3 cut(s) 44, 211, 280
BlsI GCNGC 3 cut(s) 45, 212, 281
Bme1390I CCNGG 1 cut(s) 434
Bme18I GGWCC 1 cut(s) 436
BmgT120I GGNCC 1 cut(s) 436
BmiI GGNNCC 1 cut(s) 547
BmrFI CCNGG 1 cut(s) 434
BmsI GCATC 3 cut(s) 79, 181, 681
BpiI GAAGAC 1 cut(s) 451
BpmI CTGGAG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 590, 620
Bse118I RCCGGY 2 cut(s) 554, 626
Bse1I ACTGG 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 434
BseGI GGATG 1 cut(s) 175
BseNI ACTGG 1 cut(s) 203
BseXI GCAGC 3 cut(s) 55, 197, 266
Bsh1236I CGCG 1 cut(s) 574
Bsh1285I CGRYCG 1 cut(s) 710
BsiEI CGRYCG 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 202
BsiSI CCGG 3 cut(s) 443, 555, 627
BslFI GGGAC 1 cut(s) 225
BsmFI GGGAC 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 202
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 4 cut(s) 10, 61, 396, 707
BspACI CCGC 2 cut(s) 360, 574
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 1 cut(s) 574
BspHI TCATGA 2 cut(s) 393, 682
BspLI GGNNCC 1 cut(s) 547
BspPI GGATC 1 cut(s) 391
BsrFI RCCGGY 2 cut(s) 554, 626
BsrI ACTGG 1 cut(s) 203
BssAI RCCGGY 2 cut(s) 554, 626
BssMI GATC 4 cut(s) 10, 61, 396, 707
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 1 cut(s) 606
BstC8I GCNNGC 4 cut(s) 36, 170, 500, 669
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 4 cut(s) 13, 64, 399, 710
BstMBI GATC 4 cut(s) 10, 61, 396, 707
BstMCI CGRYCG 1 cut(s) 710
BstMWI GCNNNNNNNGC 3 cut(s) 40, 279, 677
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 1 cut(s) 698
BstSCI CCNGG 1 cut(s) 432
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 55, 197, 266
BstV2I GAAGAC 1 cut(s) 451
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 4 cut(s) 36, 170, 500, 669
CciI TCATGA 2 cut(s) 393, 682
Cfr10I RCCGGY 2 cut(s) 554, 626
Cfr13I GGNCC 1 cut(s) 436
CseI GACGC 1 cut(s) 673
Csp6I GTAC 2 cut(s) 147, 423
CviAII CATG 6 cut(s) 40, 283, 394, 611, 683, 695
CviJI RGCY 6 cut(s) 34, 46, 168, 200, 279, 680
CviKI_1 RGCY 6 cut(s) 34, 46, 168, 200, 279, 680
CviQI GTAC 2 cut(s) 147, 423
DpnI GATC 4 cut(s) 12, 63, 398, 709
DpnII GATC 4 cut(s) 10, 61, 396, 707
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
Ecl136II GAGCTC 1 cut(s) 200
Eco24I GRGCYC 1 cut(s) 202
Eco32I GATATC 2 cut(s) 19, 373
Eco47I GGWCC 1 cut(s) 436
Eco53kI GAGCTC 1 cut(s) 200
EcoICRI GAGCTC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 432
EcoRV GATATC 2 cut(s) 19, 373
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 202
FaeI CATG 6 cut(s) 43, 286, 397, 614, 686, 698
FaqI GGGAC 1 cut(s) 225
FatI CATG 6 cut(s) 39, 282, 393, 610, 682, 694
FblI GTMKAC 1 cut(s) 468
Fnu4HI GCNGC 3 cut(s) 44, 211, 280
FokI GGATG 1 cut(s) 162
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 44, 211, 280
FspBI CTAG 1 cut(s) 251
GluI GCNGC 3 cut(s) 44, 211, 280
GsuI CTGGAG 1 cut(s) 186
HapII CCGG 3 cut(s) 443, 555, 627
HgaI GACGC 1 cut(s) 673
Hin1II CATG 6 cut(s) 43, 286, 397, 614, 686, 698
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HpaII CCGG 3 cut(s) 443, 555, 627
HphI GGTGA 3 cut(s) 335, 544, 626
Hpy166II GTNNAC 3 cut(s) 386, 425, 469
Hpy188I TCNGA 2 cut(s) 61, 377
Hpy188III TCNNGA 9 cut(s) 14, 117, 163, 251, 332, 394, 410, 443, 683
Hpy8I GTNNAC 3 cut(s) 386, 425, 469
Hpy99I CGWCG 2 cut(s) 371, 452
HpyAV CCTTC 2 cut(s) 320, 449
HpyCH4III ACNGT 1 cut(s) 606
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 43, 92, 172, 282, 694
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 279, 677
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 6 cut(s) 43, 286, 397, 614, 686, 698
Kpn2I TCCGGA 1 cut(s) 442
Kzo9I GATC 4 cut(s) 10, 61, 396, 707
LmnI GCTCC 3 cut(s) 197, 205, 598
LpnPI CCDG 9 cut(s) 204, 216, 357, 419, 446, 456, 568, 640, 653
Lsp1109I GCAGC 3 cut(s) 55, 197, 266
LweI GCATC 3 cut(s) 79, 181, 681
MaeI CTAG 1 cut(s) 251
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 3 cut(s) 304, 407, 559
MalI GATC 4 cut(s) 12, 63, 398, 709
MboI GATC 4 cut(s) 10, 61, 396, 707
MboII GAAGA 3 cut(s) 20, 105, 451
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 5 cut(s) 48, 156, 234, 413, 636
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 3 cut(s) 247, 279, 399
Mph1103I ATGCAT 1 cut(s) 696
MroI TCCGGA 1 cut(s) 442
MseI TTAA 1 cut(s) 290
MslI CAYNNNNRTG 1 cut(s) 69
MspI CCGG 3 cut(s) 443, 555, 627
MspR9I CCNGG 1 cut(s) 434
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 3 cut(s) 40, 279, 677
NdeII GATC 4 cut(s) 10, 61, 396, 707
NlaIII CATG 6 cut(s) 43, 286, 397, 614, 686, 698
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 1 cut(s) 698
PagI TCATGA 2 cut(s) 393, 682
PkrI GCNGC 3 cut(s) 45, 212, 281
Ple19I CGATCG 1 cut(s) 710
PsiI TTATAA 1 cut(s) 621
Psp124BI GAGCTC 1 cut(s) 202
Psp6I CCWGG 1 cut(s) 432
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 547
PspPI GGNCC 1 cut(s) 436
PvuI CGATCG 1 cut(s) 710
RsaI GTAC 2 cut(s) 148, 424
RsaNI GTAC 2 cut(s) 147, 423
RseI CAYNNNNRTG 1 cut(s) 69
SacI GAGCTC 1 cut(s) 202
SalI GTCGAC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 290
SatI GCNGC 3 cut(s) 44, 211, 280
Sau3AI GATC 4 cut(s) 10, 61, 396, 707
Sau96I GGNCC 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 434
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 3 cut(s) 79, 181, 681
SgrAI CRCCGGYG 1 cut(s) 554
SinI GGWCC 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 69
Sse9I AATT 5 cut(s) 48, 156, 234, 413, 636
SsiI CCGC 2 cut(s) 360, 574
SspMI CTAG 1 cut(s) 251
SstI GAGCTC 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 606
TaiI ACGT 1 cut(s) 453
TaqI TCGA 6 cut(s) 21, 162, 369, 468, 706, 710
TasI AATT 5 cut(s) 48, 156, 234, 413, 636
TatI WGTACW 1 cut(s) 146
Tru1I TTAA 1 cut(s) 290
Tru9I TTAA 1 cut(s) 290
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 3 cut(s) 43, 210, 279
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 21, 87, 599
TspGWI ACGGA 2 cut(s) 43, 410
VpaK11BI GGWCC 1 cut(s) 436
XapI RAATTY 1 cut(s) 413
XbaI TCTAGA 1 cut(s) 250
XceI RCATGY 1 cut(s) 698
XmiI GTMKAC 1 cut(s) 468
XspI CTAG 1 cut(s) 251
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.