RLG00000033744

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016790
Physical Location & Seq
Reverse (-)
31647599 .. 31648633
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033744

Sequence Viewer

Length: 1035 bp
ATGTATGAAGATGAAGATATCGACGATATAGAGCTCGCCATTCAGCTAATTTCCGTTTCAGATCATCAATATGAAAAAGGGCAAAGTCATGGAAATAAGGAAGTTGCTATATCTTCAAGACAGCCAATGTTGGTTTGCGATGTTTGTACTAATACAATTATCAAGAGCTTGCATCATCCCACTACTCATCTCCCAGATGGAACTCCAGTTTGCTGCGACATAATATGGAACCAAACATTTTGTCCCAGACATCTAGATGATAGTACTCCAATATGCTGTGGCTGCATGAGGTTAAAGACAAGTGATGTAACGTATATAAATCTCGGTGAAGGTCGGGAAATATGCCTAGATTGTTATTCTACCGCAATCGTCGATATCCGACAATGTGAACCTCTCATGATCCATAAATGTCACGAATTTTTCCGTACACTCAACCTGGACCTTCCGGACGACGTTCGTGTCTTCTTGGTCGACGCGAAAGAGATGGATAGACTTGACTGCGAGAAAGAAACTCCCACGCCTTTCGGGTTGAGGTTGGAGGCAGATATGGGCACCATCACCGGCGTTACAAGTTGTTCGCGGAAAGGGGATAGCGTTGTAGTGGAGCAACAGTCTCATGGTGATTATAGAACCGGCATAGCAATTTTGTTCGGTTTGCCAAAGATTATGACGCAGGCAATCTTGGCTCATGAGATGATGCATGTATGGTTTCGATCGAAAGGTATCAGATTCGGGGAACTGGAAAAGAAGCTGGAAGAAGGAATGTGTCAAGTAATAGGTTGGAAATGGTTGGATTGGCTGGACAGTGAAATTACCTCATCATCATCCGCAACTACTAGTCATGAGCAGGCTCAGTTTCTGAGAAAATTAGTACAAACATACAAGTTTGTGGTGGAAAAGCATGACTCTTATGAATATGGCCAAGGATTTAGAGAGGTTCAACGGGCAGTTGCAAGATTTGGCCTTGAAACCACAATTGATCATGTGATTACTAGGGGAAAGCTTCCAGAAGAATGCCAGTCACACTCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

345

Amino Acids

39.14

Weight (kDa)

5.24

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 156 - 330 5.4e-36 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016790)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g21570
prunus_persica Prupe.8G103200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037021
rosa_laevigata RLG00000024635 RLG00000033744
rosa_multiflora Rmu_co8219504.1_g000001 Rmu_sc0008296.1_g000003 Rmu_ssc0000062.1_g000041
rosa_roxburghii Rroxscaffold_1G00043920
rosa_samantha Rh5AG251900 Rh5BG253500 Rh5CG286200 Rh5DG262100
rosa_wichuraiana Rw5G023210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 551
AccI GTMKAC 1 cut(s) 471
AccII CGCG 2 cut(s) 476, 580
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 3 cut(s) 363, 580, 828
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 4 cut(s) 148, 265, 427, 873
AgsI TTSAA 3 cut(s) 117, 941, 968
AhlI ACTAGT 1 cut(s) 836
AjnI CCWGG 1 cut(s) 435
AluBI AGCT 6 cut(s) 34, 46, 168, 751, 1003, 1032
AluI AGCT 6 cut(s) 34, 46, 168, 751, 1003, 1032
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 1 cut(s) 394
AlwNI CAGNNNCTG 1 cut(s) 859
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 2 cut(s) 919, 961
ApeKI GCWGC 2 cut(s) 213, 282
ApoI RAATTY 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 226, 258
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 3 cut(s) 338, 550, 632
AvaII GGWCC 1 cut(s) 439
BaeGI GKGCMC 1 cut(s) 554
BalI TGGCCA 1 cut(s) 921
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 200, 269
BccI CCATC 3 cut(s) 191, 478, 563
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 1 cut(s) 979
BcoDI GTCTC 1 cut(s) 618
BcuI ACTAGT 1 cut(s) 836
BfaI CTAG 6 cut(s) 254, 347, 837, 993, 1029, 1033
BisI GCNGC 2 cut(s) 214, 283
BlsI GCNGC 2 cut(s) 215, 284
BmcAI AGTACT 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 437
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 439
BmiI GGNNCC 2 cut(s) 230, 553
BmrFI CCNGG 1 cut(s) 437
BmsI GCATC 2 cut(s) 181, 687
BpiI GAAGAC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 922
BsaWI WCCGGW 1 cut(s) 445
BsaXI ACNNNNNCTCC 2 cut(s) 596, 626
Bse118I RCCGGY 2 cut(s) 560, 632
Bse1I ACTGG 3 cut(s) 206, 744, 1018
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 1 cut(s) 922
BseGI GGATG 2 cut(s) 175, 824
BseMII CTCAG 2 cut(s) 851, 866
BseNI ACTGG 3 cut(s) 206, 744, 1018
BseSI GKGCMC 1 cut(s) 554
BseXI GCAGC 2 cut(s) 200, 269
Bsh1236I CGCG 2 cut(s) 476, 580
Bsh1285I CGRYCG 1 cut(s) 716
BshFI GGCC 2 cut(s) 921, 963
BshNI GGYRCC 1 cut(s) 551
BsiEI CGRYCG 1 cut(s) 716
BsiHKAI GWGCWC 1 cut(s) 36
BsiSI CCGG 3 cut(s) 446, 561, 633
BslFI GGGAC 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 618
BsmFI GGGAC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 1019
BsnI GGCC 2 cut(s) 921, 963
Bsp1286I GDGCHC 2 cut(s) 36, 554
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 4 cut(s) 61, 399, 713, 979
BspACI CCGC 3 cut(s) 363, 580, 828
BspANI GGCC 2 cut(s) 921, 963
BspCNI CTCAG 2 cut(s) 852, 865
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 2 cut(s) 476, 580
BspHI TCATGA 3 cut(s) 396, 688, 841
BspLI GGNNCC 2 cut(s) 230, 553
BspPI GGATC 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 551
BsrFI RCCGGY 2 cut(s) 560, 632
BsrI ACTGG 3 cut(s) 206, 744, 1018
BssAI RCCGGY 2 cut(s) 560, 632
BssECI CCNNGG 1 cut(s) 922
BssMI GATC 4 cut(s) 61, 399, 713, 979
BssT1I CCWWGG 1 cut(s) 922
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 2 cut(s) 612, 806
BstC8I GCNNGC 4 cut(s) 36, 170, 675, 849
BstDEI CTNAG 2 cut(s) 852, 860
BstF5I GGATG 2 cut(s) 175, 824
BstFNI CGCG 2 cut(s) 476, 580
BstKTI GATC 4 cut(s) 64, 402, 716, 982
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 4 cut(s) 61, 399, 713, 979
BstMCI CGRYCG 1 cut(s) 716
BstMWI GCNNNNNNNGC 2 cut(s) 282, 683
BstNI CCWGG 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 435
BstSLI GKGCMC 1 cut(s) 554
BstUI CGCG 2 cut(s) 476, 580
BstV1I GCAGC 2 cut(s) 200, 269
BstV2I GAAGAC 1 cut(s) 454
BsuRI GGCC 2 cut(s) 921, 963
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 175, 824
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 4 cut(s) 36, 170, 675, 849
CaiI CAGNNNCTG 1 cut(s) 859
CciI TCATGA 3 cut(s) 396, 688, 841
Cfr10I RCCGGY 2 cut(s) 560, 632
Cfr13I GGNCC 1 cut(s) 439
CseI GACGC 2 cut(s) 482, 679
Csp6I GTAC 4 cut(s) 147, 264, 426, 872
CviAII CATG 9 cut(s) 89, 286, 397, 617, 689, 701, 842, 902, 983
CviQI GTAC 4 cut(s) 147, 264, 426, 872
DdeI CTNAG 2 cut(s) 852, 860
DpnI GATC 4 cut(s) 63, 401, 715, 981
DpnII GATC 4 cut(s) 61, 399, 713, 979
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
EaeI YGGCCR 1 cut(s) 919
Ecl136II GAGCTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 922
Eco24I GRGCYC 1 cut(s) 36
Eco32I GATATC 2 cut(s) 19, 376
Eco47I GGWCC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 34
EcoICRI GAGCTC 1 cut(s) 34
EcoRII CCWGG 1 cut(s) 435
EcoRV GATATC 2 cut(s) 19, 376
EcoT14I CCWWGG 1 cut(s) 922
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 922
FaeI CATG 9 cut(s) 92, 289, 400, 620, 692, 704, 845, 905, 986
FaqI GGGAC 1 cut(s) 228
FatI CATG 9 cut(s) 88, 285, 396, 616, 688, 700, 841, 901, 982
FbaI TGATCA 1 cut(s) 979
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 2 cut(s) 214, 283
FokI GGATG 2 cut(s) 162, 811
FriOI GRGCYC 1 cut(s) 36
Fsp4HI GCNGC 2 cut(s) 214, 283
FspBI CTAG 6 cut(s) 254, 347, 837, 993, 1029, 1033
GluI GCNGC 2 cut(s) 214, 283
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 2 cut(s) 921, 963
HapII CCGG 3 cut(s) 446, 561, 633
HgaI GACGC 2 cut(s) 482, 679
Hin1II CATG 9 cut(s) 92, 289, 400, 620, 692, 704, 845, 905, 986
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 1001
HinfI GANTC 2 cut(s) 729, 905
HpaII CCGG 3 cut(s) 446, 561, 633
HphI GGTGA 3 cut(s) 338, 550, 632
Hpy166II GTNNAC 3 cut(s) 389, 428, 472
Hpy188I TCNGA 4 cut(s) 61, 380, 728, 861
Hpy8I GTNNAC 3 cut(s) 389, 428, 472
Hpy99I CGWCG 4 cut(s) 26, 374, 455, 476
HpyAV CCTTC 3 cut(s) 323, 452, 752
HpyCH4III ACNGT 2 cut(s) 612, 806
HpyCH4IV ACGT 2 cut(s) 311, 453
HpyCH4V TGCA 4 cut(s) 172, 285, 700, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 282, 683
HpyF3I CTNAG 2 cut(s) 852, 860
HpySE526I ACGT 2 cut(s) 311, 453
Hsp92II CATG 9 cut(s) 92, 289, 400, 620, 692, 704, 845, 905, 986
Kpn2I TCCGGA 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 4 cut(s) 61, 399, 713, 979
LmnI GCTCC 1 cut(s) 604
Lsp1109I GCAGC 2 cut(s) 200, 269
LweI GCATC 2 cut(s) 181, 687
MaeI CTAG 6 cut(s) 254, 347, 837, 993, 1029, 1033
MaeII ACGT 2 cut(s) 311, 453
MaeIII GTNAC 4 cut(s) 307, 410, 565, 1020
MalI GATC 4 cut(s) 63, 401, 715, 981
MboI GATC 4 cut(s) 61, 399, 713, 979
MboII GAAGA 6 cut(s) 20, 26, 105, 454, 767, 1022
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 2 cut(s) 36, 554
MlsI TGGCCA 1 cut(s) 921
MluCI AATT 7 cut(s) 48, 156, 416, 642, 810, 866, 975
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 4 cut(s) 403, 516, 761, 771
MnlI CCTC 6 cut(s) 282, 402, 525, 532, 826, 928
Mox20I TGGCCA 1 cut(s) 921
Mph1103I ATGCAT 1 cut(s) 702
MroI TCCGGA 1 cut(s) 445
MscI TGGCCA 1 cut(s) 921
MseI TTAA 1 cut(s) 293
MslI CAYNNNNRTG 2 cut(s) 69, 255
Msp20I TGGCCA 1 cut(s) 921
MspI CCGG 3 cut(s) 446, 561, 633
MspR9I CCNGG 1 cut(s) 437
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 1 cut(s) 1019
MvaI CCWGG 1 cut(s) 437
MvnI CGCG 2 cut(s) 476, 580
MwoI GCNNNNNNNGC 2 cut(s) 282, 683
NdeII GATC 4 cut(s) 61, 399, 713, 979
NlaIII CATG 9 cut(s) 92, 289, 400, 620, 692, 704, 845, 905, 986
NlaIV GGNNCC 2 cut(s) 230, 553
NmuCI GTSAC 2 cut(s) 410, 1020
NsiI ATGCAT 1 cut(s) 702
NspI RCATGY 1 cut(s) 704
PagI TCATGA 3 cut(s) 396, 688, 841
PctI GAATGC 1 cut(s) 1019
PfeI GAWTC 1 cut(s) 729
PkrI GCNGC 2 cut(s) 215, 284
Ple19I CGATCG 1 cut(s) 716
PleI GAGTC 1 cut(s) 899
PpsI GAGTC 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 435
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 2 cut(s) 230, 553
PspPI GGNCC 1 cut(s) 439
PstNI CAGNNNCTG 1 cut(s) 859
PvuI CGATCG 1 cut(s) 716
RsaI GTAC 4 cut(s) 148, 265, 427, 873
RsaNI GTAC 4 cut(s) 147, 264, 426, 872
RseI CAYNNNNRTG 2 cut(s) 69, 255
SacI GAGCTC 1 cut(s) 36
SalI GTCGAC 1 cut(s) 470
SaqAI TTAA 1 cut(s) 293
SatI GCNGC 2 cut(s) 214, 283
Sau3AI GATC 4 cut(s) 61, 399, 713, 979
Sau96I GGNCC 1 cut(s) 439
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 1 cut(s) 437
SduI GDGCHC 2 cut(s) 36, 554
SfaNI GCATC 2 cut(s) 181, 687
SgrAI CRCCGGYG 1 cut(s) 560
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 2 cut(s) 69, 255
SpeI ACTAGT 1 cut(s) 836
Sse9I AATT 7 cut(s) 48, 156, 416, 642, 810, 866, 975
SsiI CCGC 3 cut(s) 363, 580, 828
SspMI CTAG 6 cut(s) 254, 347, 837, 993, 1029, 1033
SstI GAGCTC 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 435
StyI CCWWGG 1 cut(s) 922
TaaI ACNGT 2 cut(s) 612, 806
TaiI ACGT 2 cut(s) 314, 456
TaqI TCGA 5 cut(s) 21, 372, 471, 712, 716
TasI AATT 7 cut(s) 48, 156, 416, 642, 810, 866, 975
TatI WGTACW 3 cut(s) 146, 263, 871
TfiI GAWTC 1 cut(s) 729
Tru1I TTAA 1 cut(s) 293
Tru9I TTAA 1 cut(s) 293
TscAI CASTG 1 cut(s) 811
TseFI GTSAC 2 cut(s) 410, 1020
TseI GCWGC 2 cut(s) 213, 282
Tsp45I GTSAC 2 cut(s) 410, 1020
TspDTI ATGAA 4 cut(s) 21, 27, 87, 927
TspGWI ACGGA 2 cut(s) 43, 413
TspRI CASTG 1 cut(s) 811
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 1 cut(s) 416
XbaI TCTAGA 1 cut(s) 253
XceI RCATGY 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 6 cut(s) 254, 347, 837, 993, 1029, 1033
ZrmI AGTACT 1 cut(s) 265
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.