FvH4_3g22020

zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016791
Physical Location & Seq
Forward (+)
15097852 .. 15101015
3164 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22020.t1

Sequence Viewer

Length: 1305 bp
ATGGACAGCAGTACTTCCCAAATTGAGATAGACATGAGGATACCTCAAGAAGCAACAACATCTCAGCAAGTGAATATGGGGTCAAATGTCCAGGTTGCAAGCAATATTTCCAGAGCCAGTGATCTACTCATGGCAGTAACTTCAAGCGCTGAAGTCAACAGAATGAAAATCAGCGAGTTAGCTTCGGATGCAATGGAAGTGCTAACTAAATTGGCCCTAGCTCAAGAACCTCTATGGCAGTGTAACATAGAGAGCAATACTGAATTTCTTAGTGATATCGAGTATCTGAGGGAATTCGGACATATAAGTGCCTCACTGATAGAAATAATTAAAATGGTTGAGGTGGGAGACTCTCAATCTGAGCTGCCGATTAACAATTCTGAATTTTTAATGGAAAGTGAATACAGGCCAGTGTTGGCAACAAATCAACCTGGAAAACCAGTTAGTTCAGAATCGTCACGAGCTATTGAATATGTCAAAATGAAAGCATTCAATCTTGTTCAATTGCTAATGGATTTGGAGCAATGGCTACTGGTGTTTCCTAATATTGTATCTAGAGCAAAATTAGTGGGAGTATTATCATCAACAAGAGTTGAGGGCAACTATGATGGAACTCTGCAAGTGATGACAGCTGAGTTTCATGCACCCACACCACTTATTCCTGCTAGAGCAAGTTATTTTGCACGGTACAGTAAACAACTGGATAGTGGAATGTGGGTTGTTGTTGATGCATCCTTGGAGAAATTGTTTCAGCTTCCATCTACTAACGTTCGAAGACAACCGTCAGGATGTTTAATTCAGGAAATGCCAAATGGATGCTCTAAGGTCATATGGGTGGAGCATGCTGAAGTGGATAACAGAATGGTTCACAATATGTTTGAGCCACTGGTCAGTTCAGGTCTTGCATTTAGCGCAAGGCGTTGGGTTTCCACTTTAGTTCGTCAATGTGAATGGTTGGCAACTTTGAATTCCACAAGCCCTCCTACATCTGATCGTGTGTTCATATCGCAAGCTGGAAGAAAAAGCTTGTTGAAGCTTACGGAGAGGATGATGAGAAGTTTTGTTGCTGATATTAGTGCTTCTACCGAAAACAAATGGAGGACATTACCTGTATTTGGTGCCGAAGATGTAAAGGTCACAACTATAACTAACTTGAATGATATTGGAAAGCCTCCAGGTACCACCTTATCATTCGCAACTTCTGTCCATCTTCCAGTTCCAAGAACTCAAATATACAATCTGCTTCGTGATGGTGAATCTCGATATATGGTATGGACAGTACAATTAAGGTTGACATCAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

435

Amino Acids

48.51

Weight (kDa)

5.48

Isoelectric Point (pI)

51.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 142 - 317 5.3e-31 START domain
PDF2_C PF25797 337 - 422 1.4e-21 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g22020 FvH4_3g22021
malus_domestica MD11G1223900.v1.1
prunus_persica Prupe.4G205900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037891
rosa_laevigata RLG00000033820
rosa_multiflora Rmu_sc0005416.1_g000025
rosa_roxburghii Rroxscaffold_1G00043030
rosa_rugosa Rorug05G0168500
rosa_samantha Rh5AG257100 Rh5BG259700 Rh5CG292700 Rh5DG269600
rosa_wichuraiana Rw5G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1178
AccB1I GGYRCC 2 cut(s) 1118, 1178
AclI AACGTT 1 cut(s) 768
AcsI RAATTY 4 cut(s) 263, 293, 383, 967
AcuI CTGAAG 2 cut(s) 171, 867
AfaI GTAC 4 cut(s) 13, 689, 1180, 1281
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 1 cut(s) 1115
AgsI TTSAA 7 cut(s) 144, 470, 493, 503, 967, 1033, 1156
AjnI CCWGG 3 cut(s) 90, 430, 1174
AluBI AGCT 9 cut(s) 182, 221, 364, 464, 632, 754, 1013, 1026, 1036
AluI AGCT 9 cut(s) 182, 221, 364, 464, 632, 754, 1013, 1026, 1036
Alw26I GTCTC 1 cut(s) 342
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 2 cut(s) 213, 407
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 4 cut(s) 263, 293, 383, 967
Asp700I GAANNNNTTC 1 cut(s) 488
Asp718I GGTACC 1 cut(s) 1178
AspLEI GCGC 2 cut(s) 149, 914
AspS9I GGNCC 1 cut(s) 214
AsuHPI GGTGA 1 cut(s) 1265
AsuII TTCGAA 1 cut(s) 772
BanI GGYRCC 2 cut(s) 1118, 1178
BauI CACGAG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 781
BbvI GCAGC 1 cut(s) 351
BccI CCATC 4 cut(s) 602, 766, 1215, 1244
BciT130I CCWGG 3 cut(s) 92, 432, 1176
BciVI GTATCC 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 342
BfaI CTAG 3 cut(s) 218, 555, 666
BfoI RGCGCY 1 cut(s) 150
BfuI GTATCC 1 cut(s) 33
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
BmcAI AGTACT 1 cut(s) 13
Bme1390I CCNGG 3 cut(s) 92, 432, 1176
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 2 cut(s) 1120, 1180
BmrFI CCNGG 3 cut(s) 92, 432, 1176
BmsI GCATC 4 cut(s) 178, 718, 740, 806
BoxI GACNNNNGTC 1 cut(s) 781
BpiI GAAGAC 1 cut(s) 781
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 1 cut(s) 1158
Bpu14I TTCGAA 1 cut(s) 772
BpuEI CTTGAG 2 cut(s) 30, 207
BsaJI CCNNGG 1 cut(s) 735
BsaXI ACNNNNNCTCC 4 cut(s) 731, 761, 964, 994
Bsc4I CCNNNNNNNGG 1 cut(s) 1115
Bse1I ACTGG 7 cut(s) 117, 410, 440, 537, 705, 891, 1214
Bse3DI GCAATG 2 cut(s) 198, 530
BseBI CCWGG 3 cut(s) 92, 432, 1176
BseDI CCNNGG 1 cut(s) 735
BseGI GGATG 5 cut(s) 193, 731, 794, 821, 1053
BseLI CCNNNNNNNGG 1 cut(s) 1115
BseMI GCAATG 2 cut(s) 198, 530
BseMII CTCAG 4 cut(s) 77, 278, 351, 624
BseNI ACTGG 7 cut(s) 117, 410, 440, 537, 705, 891, 1214
BseXI GCAGC 1 cut(s) 351
BshFI GGCC 2 cut(s) 215, 409
BshNI GGYRCC 2 cut(s) 1118, 1178
BslI CCNNNNNNNGG 1 cut(s) 1115
BsmAI GTCTC 1 cut(s) 342
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 2 cut(s) 215, 409
Bsp119I TTCGAA 1 cut(s) 772
Bsp143I GATC 2 cut(s) 121, 991
BspANI GGCC 2 cut(s) 215, 409
BspCNI CTCAG 4 cut(s) 76, 279, 352, 625
BspLI GGNNCC 2 cut(s) 1120, 1180
BspT104I TTCGAA 1 cut(s) 772
BspT107I GGYRCC 2 cut(s) 1118, 1178
BsrDI GCAATG 2 cut(s) 198, 530
BsrI ACTGG 7 cut(s) 117, 410, 440, 537, 705, 891, 1214
BssECI CCNNGG 1 cut(s) 735
BssMI GATC 2 cut(s) 121, 991
BssSI CACGAG 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 735
Bst2BI CACGAG 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 92, 432, 1176
Bst4CI ACNGT 4 cut(s) 687, 692, 783, 1279
BstBI TTCGAA 1 cut(s) 772
BstC8I GCNNGC 3 cut(s) 100, 843, 1011
BstDEI CTNAG 6 cut(s) 63, 269, 287, 360, 633, 822
BstF5I GGATG 5 cut(s) 193, 731, 794, 821, 1053
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 2 cut(s) 149, 914
BstKTI GATC 2 cut(s) 124, 994
BstMAI GTCTC 1 cut(s) 342
BstMBI GATC 2 cut(s) 121, 991
BstMWI GCNNNNNNNGC 2 cut(s) 188, 911
BstNI CCWGG 3 cut(s) 92, 432, 1176
BstNSI RCATGY 1 cut(s) 845
BstPAI GACNNNNGTC 1 cut(s) 781
BstSCI CCNGG 3 cut(s) 90, 430, 1174
BstV1I GCAGC 1 cut(s) 351
BstV2I GAAGAC 1 cut(s) 781
BsuI GTATCC 1 cut(s) 33
BsuRI GGCC 2 cut(s) 215, 409
BtsCI GGATG 5 cut(s) 193, 731, 794, 821, 1053
BtsI GCAGTG 1 cut(s) 245
BtsIMutI CAGTG 5 cut(s) 124, 245, 314, 417, 884
Cac8I GCNNGC 3 cut(s) 100, 843, 1011
CfoI GCGC 2 cut(s) 149, 914
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 4 cut(s) 12, 688, 1179, 1280
CspCI CAANNNNNGTGG 2 cut(s) 549, 584
CviAII CATG 4 cut(s) 34, 130, 641, 842
CviQI GTAC 4 cut(s) 12, 688, 1179, 1280
DdeI CTNAG 6 cut(s) 63, 269, 287, 360, 633, 822
DpnI GATC 2 cut(s) 123, 993
DpnII GATC 2 cut(s) 121, 991
Eco130I CCWWGG 1 cut(s) 735
Eco32I GATATC 1 cut(s) 277
Eco47III AGCGCT 1 cut(s) 148
Eco57I CTGAAG 2 cut(s) 171, 867
EcoRI GAATTC 2 cut(s) 293, 967
EcoRII CCWGG 3 cut(s) 90, 430, 1174
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 1 cut(s) 733
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 4 cut(s) 37, 133, 644, 845
FatI CATG 4 cut(s) 33, 129, 640, 841
FauNDI CATATG 1 cut(s) 830
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 5 cut(s) 200, 718, 801, 828, 1060
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 3 cut(s) 218, 555, 666
GlaI GCGC 2 cut(s) 148, 913
GluI GCNGC 1 cut(s) 365
GsuI CTGGAG 1 cut(s) 1158
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 2 cut(s) 215, 409
HhaI GCGC 2 cut(s) 149, 914
Hin1II CATG 4 cut(s) 37, 133, 644, 845
Hin6I GCGC 2 cut(s) 147, 912
HinP1I GCGC 2 cut(s) 147, 912
HincII GTYRAC 2 cut(s) 157, 1293
HindII GTYRAC 2 cut(s) 157, 1293
HindIII AAGCTT 2 cut(s) 1024, 1034
HinfI GANTC 3 cut(s) 350, 452, 1256
HphI GGTGA 1 cut(s) 1265
Hpy166II GTNNAC 4 cut(s) 157, 695, 868, 1293
Hpy188I TCNGA 7 cut(s) 187, 288, 299, 361, 382, 451, 991
Hpy188III TCNNGA 9 cut(s) 47, 111, 224, 459, 555, 786, 800, 1247, 1260
Hpy8I GTNNAC 4 cut(s) 157, 695, 868, 1293
HpyCH4III ACNGT 4 cut(s) 687, 692, 783, 1279
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 7 cut(s) 98, 191, 619, 644, 683, 731, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 911
HpyF3I CTNAG 6 cut(s) 63, 269, 287, 360, 633, 822
HpySE526I ACGT 1 cut(s) 768
Hsp92II CATG 4 cut(s) 37, 133, 644, 845
HspAI GCGC 2 cut(s) 147, 912
KpnI GGTACC 1 cut(s) 1182
Kzo9I GATC 2 cut(s) 121, 991
LmnI GCTCC 2 cut(s) 520, 838
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 4 cut(s) 178, 718, 740, 806
MaeI CTAG 3 cut(s) 218, 555, 666
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 4 cut(s) 136, 242, 456, 1135
MalI GATC 2 cut(s) 123, 993
MboI GATC 2 cut(s) 121, 991
MboII GAAGA 4 cut(s) 786, 1029, 1136, 1202
MfeI CAATTG 1 cut(s) 503
MlyI GAGTC 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 488
MseI TTAA 6 cut(s) 330, 372, 389, 794, 1286, 1303
MslI CAYNNNNRTG 2 cut(s) 306, 833
MspA1I CMGCKG 1 cut(s) 632
MspR9I CCNGG 3 cut(s) 92, 432, 1176
MunI CAATTG 1 cut(s) 503
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 3 cut(s) 92, 432, 1176
MwoI GCNNNNNNNGC 2 cut(s) 188, 911
NdeI CATATG 1 cut(s) 830
NdeII GATC 2 cut(s) 121, 991
NlaIII CATG 4 cut(s) 37, 133, 644, 845
NlaIV GGNNCC 2 cut(s) 1120, 1180
NmuCI GTSAC 2 cut(s) 456, 1135
NsiI ATGCAT 1 cut(s) 733
NspI RCATGY 1 cut(s) 845
NspV TTCGAA 1 cut(s) 772
PaeI GCATGC 1 cut(s) 845
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 452, 1256
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 1 cut(s) 344
PpsI GAGTC 1 cut(s) 344
PshAI GACNNNNGTC 1 cut(s) 781
Psp1406I AACGTT 1 cut(s) 768
Psp6I CCWGG 3 cut(s) 90, 430, 1174
PspGI CCWGG 3 cut(s) 90, 430, 1174
PspN4I GGNNCC 2 cut(s) 1120, 1180
PspPI GGNCC 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 4 cut(s) 13, 689, 1180, 1281
RsaNI GTAC 4 cut(s) 12, 688, 1179, 1280
RseI CAYNNNNRTG 2 cut(s) 306, 833
SaqAI TTAA 6 cut(s) 330, 372, 389, 794, 1286, 1303
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 2 cut(s) 121, 991
Sau96I GGNCC 1 cut(s) 214
ScaI AGTACT 1 cut(s) 13
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 3 cut(s) 92, 432, 1176
SfaNI GCATC 4 cut(s) 178, 718, 740, 806
SfuI TTCGAA 1 cut(s) 772
SmiMI CAYNNNNRTG 2 cut(s) 306, 833
SmlI CTYRAG 2 cut(s) 45, 222
SmoI CTYRAG 2 cut(s) 45, 222
SphI GCATGC 1 cut(s) 845
SspI AATATT 2 cut(s) 106, 547
SspMI CTAG 3 cut(s) 218, 555, 666
StyD4I CCNGG 3 cut(s) 90, 430, 1174
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 4 cut(s) 687, 692, 783, 1279
TaiI ACGT 1 cut(s) 771
TaqI TCGA 3 cut(s) 279, 772, 1261
TatI WGTACW 2 cut(s) 11, 1279
TfiI GAWTC 2 cut(s) 452, 1256
Tru1I TTAA 6 cut(s) 330, 372, 389, 794, 1286, 1303
Tru9I TTAA 6 cut(s) 330, 372, 389, 794, 1286, 1303
TscAI CASTG 5 cut(s) 124, 245, 321, 417, 891
TseFI GTSAC 2 cut(s) 456, 1135
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 2 cut(s) 456, 1135
TspDTI ATGAA 4 cut(s) 179, 497, 629, 991
TspGWI ACGGA 1 cut(s) 1055
TspRI CASTG 5 cut(s) 124, 245, 321, 417, 891
XapI RAATTY 4 cut(s) 263, 293, 383, 967
XbaI TCTAGA 1 cut(s) 554
XceI RCATGY 1 cut(s) 845
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 3 cut(s) 218, 555, 666
ZrmI AGTACT 1 cut(s) 13
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.