Rmu_sc0005416.1_g000025

zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005416.1
OG0016791
Physical Location & Seq
Forward (+)
119797 .. 123508
3712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005416.1_g000025.1.cds

Sequence Viewer

Length: 1545 bp
atgcagatggacagcagtatttcacaaattgaggtagacatgaggatacctcaacatgcaacaccatctcagcaagtctcattggagaatttggggtcaaatgtccaggttgcaagcaatatttccagagccagtgatctactcatggcagtaacttcaagtgctgaaatcaacagaatgaaaatcagtgagttagcttcggaagcaatggaagagctaactaagttggccctagctcgagaacctctatggcagtgtgacatagagagcaatactgaatttcttagtgatatcgagtatctgagggaatttggacacataagtacgtcattgatagaaatcattagaatggttgaggtgggagactctcaatctgacctgccaagattcggaagcattaacaattctgaattttcgatggaaagtgaatataggccaatatcgacaacaaatcaacctggaaaaccagttagttcggaatcttcacgagctattgactatgtcaaaatgaaagcagtcaacattgttcaattgctaatggatttgaagcaatggctactggtgtttcctaatattgtatctagagcaaaagtagtgggagtattatcatcaacaagattcgaaggcaactatgatggaactctgcaagtggtcatatgggtggagcatgtggaagtggataatagaatggttcacaatatgtttaagccactggtcagttctggttttgcatttagcgcaaagcgttgggtttccactttagttcgacaatgtgaatggttggcaactttaaattctacaagccctcctacaacagattgtactggaagaaaaagcttgttgaagctttcggagaggatgatgagaagttttgttgctgatattagtgcttctacagaaaacaaatggagggcactacctgtatttggtgccgaagatgtaaaggtcacaactaaaactagcttaaataaccctggaaagcctccaggtaccaccttattatttgctacttctgtccatcttccagtcccaagaactcaaatatacaatctgcttcgcaatggtgaatctcgaatcatgtgggatatgatgtcgtatgaaaaagatacaagagaaattgcttatatcgcaactggggaccctccaggaaatcgcgtttcgataattgaagtagataacggtgtaacgaaggagattgaagttttctacttccaggagagttacacagactcaaatggctcgtacatagtttacgcaccgattgaccagttcgccatgtctgctctcttggatggctcaaacccagaccatgttgctattctcgcatccgggttttccatccttcctgatagaccaatggccaatgatgaatctggtggaagtctgttgactcttgcgttgcacatcaccgaaggaggagcaacggaggaatatatccctcctcaaacttttcaaaatatactcaacattattgctggaactgtgattcaaatcacagaagtattatcaaatgatggagaaaatagtgttgaatcagagcagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

514

Amino Acids

56.99

Weight (kDa)

4.76

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g22020 FvH4_3g22021
malus_domestica MD11G1223900.v1.1
prunus_persica Prupe.4G205900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037891
rosa_laevigata RLG00000033820
rosa_multiflora Rmu_sc0005416.1_g000025
rosa_roxburghii Rroxscaffold_1G00043030
rosa_rugosa Rorug05G0168500
rosa_samantha Rh5AG257100 Rh5BG259700 Rh5CG292700 Rh5DG269600
rosa_wichuraiana Rw5G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 387
Acc65I GGTACC 1 cut(s) 989
AccB1I GGYRCC 2 cut(s) 929, 989
AccI GTMKAC 1 cut(s) 36
AccII CGCG 1 cut(s) 1155
AcoI YGGCCR 1 cut(s) 1359
AcsI RAATTY 5 cut(s) 88, 278, 308, 410, 793
AfaI GTAC 4 cut(s) 325, 823, 991, 1244
AfiI CCNNNNNNNGG 2 cut(s) 389, 926
AgsI TTSAA 9 cut(s) 159, 530, 547, 844, 1169, 1199, 1454, 1490, 1532
AjnI CCWGG 6 cut(s) 105, 457, 973, 985, 1144, 1212
AluBI AGCT 7 cut(s) 197, 217, 236, 491, 837, 847, 963
AluI AGCT 7 cut(s) 197, 217, 236, 491, 837, 847, 963
Alw26I GTCTC 2 cut(s) 82, 357
Ama87I CYCGRG 1 cut(s) 237
AoxI GGCC 3 cut(s) 228, 434, 1359
ApoI RAATTY 5 cut(s) 88, 278, 308, 410, 793
Asp718I GGTACC 1 cut(s) 989
AspLEI GCGC 1 cut(s) 740
AspS9I GGNCC 2 cut(s) 229, 1138
AsuC2I CCSGG 1 cut(s) 1330
AsuHPI GGTGA 2 cut(s) 1076, 1399
AsuII TTCGAA 1 cut(s) 621
AvaI CYCGRG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 1138
BaeGI GKGCMC 1 cut(s) 916
BalI TGGCCA 1 cut(s) 1361
BanI GGYRCC 2 cut(s) 929, 989
BauI CACGAG 1 cut(s) 486
BccI CCATC 7 cut(s) 73, 412, 629, 1026, 1286, 1346, 1508
BciT130I CCWGG 6 cut(s) 107, 459, 975, 987, 1146, 1214
BciVI GTATCC 1 cut(s) 39
BcnI CCSGG 1 cut(s) 1330
BcoDI GTCTC 2 cut(s) 82, 357
BfaI CTAG 3 cut(s) 233, 582, 960
BfmI CTRYAG 1 cut(s) 894
BfuAI ACCTGC 1 cut(s) 387
BfuI GTATCC 1 cut(s) 39
Bme1390I CCNGG 7 cut(s) 107, 459, 975, 987, 1146, 1214, 1330
Bme18I GGWCC 1 cut(s) 1138
BmeT110I CYCGRG 1 cut(s) 237
BmgT120I GGNCC 2 cut(s) 229, 1138
BmiI GGNNCC 4 cut(s) 931, 991, 1139, 1140
BmrFI CCNGG 7 cut(s) 107, 459, 975, 987, 1146, 1214, 1330
BmrI ACTGGG 1 cut(s) 1143
BmsI GCATC 1 cut(s) 1334
BmuI ACTGGG 1 cut(s) 1143
BplI GAGNNNNNCTC 2 cut(s) 34, 66
BpmI CTGGAG 2 cut(s) 969, 1128
Bpu14I TTCGAA 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 1330
BsaBI GATNNNNATC 2 cut(s) 338, 1490
BsaJI CCNNGG 1 cut(s) 973
BsaXI ACNNNNNCTCC 2 cut(s) 790, 820
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 926
Bse1I ACTGG 8 cut(s) 132, 467, 564, 717, 829, 1025, 1138, 1267
Bse3DI GCAATG 3 cut(s) 213, 557, 1066
Bse8I GATNNNNATC 2 cut(s) 338, 1490
BseBI CCWGG 6 cut(s) 107, 459, 975, 987, 1146, 1214
BseDI CCNNGG 1 cut(s) 973
BseGI GGATG 4 cut(s) 864, 1297, 1325, 1338
BseJI GATNNNNATC 2 cut(s) 338, 1490
BseLI CCNNNNNNNGG 2 cut(s) 389, 926
BseMI GCAATG 3 cut(s) 213, 557, 1066
BseMII CTCAG 2 cut(s) 83, 293
BseNI ACTGG 8 cut(s) 132, 467, 564, 717, 829, 1025, 1138, 1267
BseRI GAGGAG 2 cut(s) 1431, 1431
BseSI GKGCMC 1 cut(s) 916
Bsh1236I CGCG 1 cut(s) 1155
BshFI GGCC 3 cut(s) 230, 436, 1361
BshNI GGYRCC 2 cut(s) 929, 989
BsiHKCI CYCGRG 1 cut(s) 237
BsiSI CCGG 1 cut(s) 1329
BslFI GGGAC 2 cut(s) 1013, 1151
BslI CCNNNNNNNGG 2 cut(s) 389, 926
BsmAI GTCTC 2 cut(s) 82, 357
BsmFI GGGAC 2 cut(s) 1013, 1151
BsnI GGCC 3 cut(s) 230, 436, 1361
BsoBI CYCGRG 1 cut(s) 237
Bsp119I TTCGAA 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 916
Bsp143I GATC 1 cut(s) 136
BspANI GGCC 3 cut(s) 230, 436, 1361
BspCNI CTCAG 2 cut(s) 82, 294
BspFNI CGCG 1 cut(s) 1155
BspLI GGNNCC 4 cut(s) 931, 991, 1139, 1140
BspMI ACCTGC 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 207
BspT104I TTCGAA 1 cut(s) 621
BspT107I GGYRCC 2 cut(s) 929, 989
BsrDI GCAATG 3 cut(s) 213, 557, 1066
BsrI ACTGG 8 cut(s) 132, 467, 564, 717, 829, 1025, 1138, 1267
BssECI CCNNGG 1 cut(s) 973
BssMI GATC 1 cut(s) 136
BssSI CACGAG 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 6 cut(s) 107, 459, 975, 987, 1146, 1214
Bst4CI ACNGT 2 cut(s) 1181, 1483
Bst6I CTCTTC 1 cut(s) 207
BstBI TTCGAA 1 cut(s) 621
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 4 cut(s) 69, 222, 284, 302
BstF5I GGATG 4 cut(s) 864, 1297, 1325, 1338
BstFNI CGCG 1 cut(s) 1155
BstHHI GCGC 1 cut(s) 740
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 82, 357
BstMBI GATC 1 cut(s) 136
BstMWI GCNNNNNNNGC 5 cut(s) 203, 737, 1127, 1280, 1322
BstNI CCWGG 6 cut(s) 107, 459, 975, 987, 1146, 1214
BstNSI RCATGY 2 cut(s) 59, 671
BstSCI CCNGG 7 cut(s) 105, 457, 973, 985, 1144, 1212, 1328
BstSFI CTRYAG 1 cut(s) 894
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 1 cut(s) 1155
BsuI GTATCC 1 cut(s) 39
BsuRI GGCC 3 cut(s) 230, 436, 1361
BtsCI GGATG 4 cut(s) 864, 1297, 1325, 1338
BtsI GCAGTG 1 cut(s) 260
BtsIMutI CAGTG 4 cut(s) 139, 193, 260, 710
BveI ACCTGC 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 115
CfoI GCGC 1 cut(s) 740
Cfr13I GGNCC 2 cut(s) 229, 1138
Csp6I GTAC 4 cut(s) 324, 822, 990, 1243
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 7 cut(s) 40, 56, 145, 668, 1078, 1276, 1310
CviQI GTAC 4 cut(s) 324, 822, 990, 1243
DdeI CTNAG 4 cut(s) 69, 222, 284, 302
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
DraI TTTAAA 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 1359
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 1138
Eco88I CYCGRG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 1138
EcoRII CCWGG 6 cut(s) 105, 457, 973, 985, 1144, 1212
EcoRV GATATC 1 cut(s) 292
FaeI CATG 7 cut(s) 43, 59, 148, 671, 1081, 1279, 1313
FaqI GGGAC 2 cut(s) 1013, 1151
FatI CATG 7 cut(s) 39, 55, 144, 667, 1077, 1275, 1309
FauNDI CATATG 1 cut(s) 656
FblI GTMKAC 1 cut(s) 36
FokI GGATG 4 cut(s) 871, 1304, 1312, 1325
FspBI CTAG 3 cut(s) 233, 582, 960
GlaI GCGC 1 cut(s) 739
GsuI CTGGAG 2 cut(s) 969, 1128
HaeIII GGCC 3 cut(s) 230, 436, 1361
HapII CCGG 1 cut(s) 1329
HhaI GCGC 1 cut(s) 740
Hin1II CATG 7 cut(s) 43, 59, 148, 671, 1081, 1279, 1313
Hin6I GCGC 1 cut(s) 738
HinP1I GCGC 1 cut(s) 738
HincII GTYRAC 2 cut(s) 520, 1389
HindII GTYRAC 2 cut(s) 520, 1389
HindIII AAGCTT 2 cut(s) 835, 845
HpaII CCGG 1 cut(s) 1329
HphI GGTGA 2 cut(s) 1076, 1399
Hpy166II GTNNAC 5 cut(s) 37, 520, 694, 1252, 1389
Hpy188I TCNGA 8 cut(s) 202, 303, 376, 392, 409, 478, 853, 1537
Hpy188III TCNNGA 6 cut(s) 126, 239, 486, 582, 1071, 1346
Hpy8I GTNNAC 5 cut(s) 37, 520, 694, 1252, 1389
HpyAV CCTTC 4 cut(s) 617, 1183, 1352, 1406
HpyCH4III ACNGT 2 cut(s) 1181, 1483
HpyCH4IV ACGT 1 cut(s) 326
HpyCH4V TGCA 6 cut(s) 4, 59, 113, 646, 731, 1402
HpyF10VI GCNNNNNNNGC 5 cut(s) 203, 737, 1127, 1280, 1322
HpyF3I CTNAG 4 cut(s) 69, 222, 284, 302
HpySE526I ACGT 1 cut(s) 326
Hsp92II CATG 7 cut(s) 43, 59, 148, 671, 1081, 1279, 1313
HspAI GCGC 1 cut(s) 738
KflI GGGWCCC 1 cut(s) 1138
KpnI GGTACC 1 cut(s) 993
Kzo9I GATC 1 cut(s) 136
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 664, 1418
LweI GCATC 1 cut(s) 1334
MaeI CTAG 3 cut(s) 233, 582, 960
MaeII ACGT 1 cut(s) 326
MaeIII GTNAC 5 cut(s) 151, 257, 946, 1183, 1220
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MboII GAAGA 5 cut(s) 224, 474, 840, 947, 1013
MfeI CAATTG 1 cut(s) 530
MhlI GDGCHC 1 cut(s) 916
MlsI TGGCCA 1 cut(s) 1361
MluNI TGGCCA 1 cut(s) 1361
MlyI GAGTC 3 cut(s) 359, 1223, 1384
Mox20I TGGCCA 1 cut(s) 1361
MscI TGGCCA 1 cut(s) 1361
MseI TTAA 4 cut(s) 399, 705, 791, 965
MslI CAYNNNNRTG 2 cut(s) 347, 659
Msp20I TGGCCA 1 cut(s) 1361
MspI CCGG 1 cut(s) 1329
MspR9I CCNGG 7 cut(s) 107, 459, 975, 987, 1146, 1214, 1330
MunI CAATTG 1 cut(s) 530
MvaI CCWGG 6 cut(s) 107, 459, 975, 987, 1146, 1214
MvnI CGCG 1 cut(s) 1155
MwoI GCNNNNNNNGC 5 cut(s) 203, 737, 1127, 1280, 1322
NciI CCSGG 1 cut(s) 1330
NdeI CATATG 1 cut(s) 656
NdeII GATC 1 cut(s) 136
NlaIII CATG 7 cut(s) 43, 59, 148, 671, 1081, 1279, 1313
NlaIV GGNNCC 4 cut(s) 931, 991, 1139, 1140
NmuCI GTSAC 2 cut(s) 257, 946
NspI RCATGY 2 cut(s) 59, 671
NspV TTCGAA 1 cut(s) 621
PaeR7I CTCGAG 1 cut(s) 237
PciSI GCTCTTC 1 cut(s) 207
PfeI GAWTC 8 cut(s) 387, 479, 618, 1067, 1074, 1370, 1486, 1532
PflFI GACNNNGTC 1 cut(s) 500
PfoI TCCNGGA 2 cut(s) 1144, 1212
PleI GAGTC 3 cut(s) 359, 1223, 1384
PpsI GAGTC 3 cut(s) 359, 1223, 1384
PpuMI RGGWCCY 1 cut(s) 1138
Psp5II RGGWCCY 1 cut(s) 1138
Psp6I CCWGG 6 cut(s) 105, 457, 973, 985, 1144, 1212
PspGI CCWGG 6 cut(s) 105, 457, 973, 985, 1144, 1212
PspN4I GGNNCC 4 cut(s) 931, 991, 1139, 1140
PspPI GGNCC 2 cut(s) 229, 1138
PspPPI RGGWCCY 1 cut(s) 1138
PsyI GACNNNGTC 1 cut(s) 500
RsaI GTAC 4 cut(s) 325, 823, 991, 1244
RsaNI GTAC 4 cut(s) 324, 822, 990, 1243
RseI CAYNNNNRTG 2 cut(s) 347, 659
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 4 cut(s) 399, 705, 791, 965
Sau3AI GATC 1 cut(s) 136
Sau96I GGNCC 2 cut(s) 229, 1138
SchI GAGTC 3 cut(s) 359, 1223, 1384
ScrFI CCNGG 7 cut(s) 107, 459, 975, 987, 1146, 1214, 1330
SduI GDGCHC 1 cut(s) 916
SfaNI GCATC 1 cut(s) 1334
SfcI CTRYAG 1 cut(s) 894
Sfr274I CTCGAG 1 cut(s) 237
SfuI TTCGAA 1 cut(s) 621
SinI GGWCC 1 cut(s) 1138
SlaI CTCGAG 1 cut(s) 237
SmiMI CAYNNNNRTG 2 cut(s) 347, 659
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SspI AATATT 2 cut(s) 121, 574
SspMI CTAG 3 cut(s) 233, 582, 960
StyD4I CCNGG 7 cut(s) 105, 457, 973, 985, 1144, 1212, 1328
TaaI ACNGT 2 cut(s) 1181, 1483
TaiI ACGT 1 cut(s) 329
TaqI TCGA 8 cut(s) 238, 294, 416, 443, 621, 766, 1072, 1160
TatI WGTACW 1 cut(s) 821
TfiI GAWTC 8 cut(s) 387, 479, 618, 1067, 1074, 1370, 1486, 1532
Tru1I TTAA 4 cut(s) 399, 705, 791, 965
Tru9I TTAA 4 cut(s) 399, 705, 791, 965
TscAI CASTG 4 cut(s) 139, 193, 260, 717
TseFI GTSAC 2 cut(s) 257, 946
Tsp45I GTSAC 2 cut(s) 257, 946
TspDTI ATGAA 4 cut(s) 194, 524, 1113, 1383
TspGWI ACGGA 1 cut(s) 1439
TspRI CASTG 4 cut(s) 139, 193, 260, 717
Tth111I GACNNNGTC 1 cut(s) 500
VpaK11BI GGWCC 1 cut(s) 1138
XapI RAATTY 5 cut(s) 88, 278, 308, 410, 793
XbaI TCTAGA 1 cut(s) 581
XceI RCATGY 2 cut(s) 59, 671
XhoI CTCGAG 1 cut(s) 237
XmiI GTMKAC 1 cut(s) 36
XspI CTAG 3 cut(s) 233, 582, 960
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.