Rh5DG269600

zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0016791
Physical Location & Seq
Forward (+)
32669618 .. 32675032
5415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG269600.1

Sequence Viewer

Length: 1971 bp
ATGCTACAGCTCAAAACTTCCGGAAGAGGACTGACCCGCAGTAATATTACGAACGGTGGAGTTGTTGATCATCGGGAAAACATGAAATTTTCTGAGAATTTTGAAGAAAGAGAACCTAGTCATGAGGTCTCTAATTTTGTGGGAGTGTCTGCAGCTTCAAGTAGCGGAATAGCGAATGGAGGTCAATTAGGGTTAAACACCAGAGAGGAATTACAGATGGACAGCAGTATTTCACAAATTGAGGTAGACATGAGGATACCTCAACATGCAACACCATCTCAGCAAGTCTCATTGGAGAATTTGGGGTCAAATGTCCAGGTTGCAAGCAATATTTCCAGAGCCAGTGATCTACTCATGGCAGTAACTTCAAGAGCTGAAATCAACAGAATGAAAATCAGTGAGTTAGCTTCGGAAGCAATGGAAGAGCTAACTAAGTTGGCCCTAGCTCGAGAACCTCTATGGCAGTGTGACATAGAGAGCAATACTGAATTTCTTAGTGATATCGAGTATCTGAGGGAATTTGGACACATAAGTACGTCATTGATAGAAATCATTAGAATGGTTGAGGTGGGAGACTCTCAATCTGACCTGCCAAGATTCGGAAGCATTAACAATTCTGAATTTTCGATGGAAAGTGAATATAGGCCAATATCGACAACAAATCAACCTGGAAAACCAGTTAGTTCGGAATCTTCACGAGCTATTGACTATGTCAAAATGAAAGCAGTCAACATTGTTCAATTGCTAATGGATTTGAAGCAATGGCTACTGGTGTTTCCTAATATTGTATCTAGAGCAAAAGTAGTGGGAGTATTATCATCAACAAGATTCGAAGGCAACTATGATGGAACTCTGCAAGTGATGACAGCTGAGTTTCATGCCCCAACACCACTTATTCCCGCTAGAGAAAGTTATTTTGCAAGGTATAGTAAACAGCTGGACAGGGGAATGTGGGTAGTTGTTGATGTATCCTTGGAAAAATTGTTTCAGCTTCCATCAACAAACTTTCGAAGACAACCATCAGGATGTTTAATTCAGGAAATGTCAAATGGATGCTCTAAGGTCATATGGGTGGAGCATGTGGAAGTGGATAATAGAATGGTTCACAATATGTTTAAGCCACTGGTCAGTTCTGGTTTTGCATTTAGCGCAAAGCGTTGGGTTTCCACTTTAGTTCGACAATGTGAATGGTTGGCAACTTTAAATTCTACAAGCCCTCCTACAACAGATTGTATGTTCGTATCTCGTGCTGGAAGAAAAAGCTTGTTGAAGCTTTCGGAGAGGATGATGAGAAGTTTTGTTGCTGATATCAGTGCTTCTACAGAAAACAAATGGAGGGCACTACCTGTATTTGGTGCCGAAGATGTAAAGGTCACAACTAAAACTAGCTTAAATGACCCTGGAAAGCCTCCAGGTACCACCTTATTATTTGCTACTTCTGTCCATCTTCCAGTCCCAAGAACTCAAATATACAATCTGCTTCGCAATGGTGAATCTCGAATCATGTGGGATATGATGTCGTATGAAAAAGATACAAGAGAAATTGCTTATATCGCAACTGGGGACCCTCCAGGAAATCGCGTTTCGATAATTGAAGTAGATAATGGTGTAACGAAGGAGATTGAAGTTTTCTACTTCCAGGAGAGTTACACAGACTCAAATGGCTCGTACATAGTTTACGCACCGATTGACCAGTTCGCCATGTCTGCTCTCTTGGATGGCTCAAACCCAGACCATGTTGCTATTCTCGCATCCGGGTTTTCCATCCTTCCTGATAGACCAATGGCCAATGATGAATCTGGTGGAAGTCTGTTGACTCTTGCGTTGCACATCACCGAAGGAGGAGCAACGGAGGAATATATCCCTCCTCAAACTTTTCAAAATATACTCAACATTATTGCTGGAACTGTGATTCAAATCACAGAAGTATTATCAAATGATGGAGAAAATAGTGTTGAATCAGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

656

Amino Acids

72.8

Weight (kDa)

5.02

Isoelectric Point (pI)

47.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 211 - 396 1e-33 START domain
PDF2_C PF25797 416 - 644 1.6e-59 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g22020 FvH4_3g22021
malus_domestica MD11G1223900.v1.1
prunus_persica Prupe.4G205900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037891
rosa_laevigata RLG00000033820
rosa_multiflora Rmu_sc0005416.1_g000025
rosa_roxburghii Rroxscaffold_1G00043030
rosa_rugosa Rorug05G0168500
rosa_samantha Rh5AG257100 Rh5BG259700 Rh5CG292700 Rh5DG269600
rosa_wichuraiana Rw5G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 597
Acc65I GGTACC 1 cut(s) 1415
AccB1I GGYRCC 2 cut(s) 1355, 1415
AccI GTMKAC 1 cut(s) 246
AccII CGCG 1 cut(s) 1581
AccIII TCCGGA 1 cut(s) 20
AciI CCGC 3 cut(s) 37, 165, 900
AcoI YGGCCR 1 cut(s) 1785
AcsI RAATTY 7 cut(s) 86, 97, 298, 488, 518, 620, 1204
AfaI GTAC 3 cut(s) 535, 1417, 1670
AfiI CCNNNNNNNGG 2 cut(s) 599, 1352
AjnI CCWGG 6 cut(s) 315, 667, 1399, 1411, 1570, 1638
Alw26I GTCTC 3 cut(s) 133, 292, 567
Ama87I CYCGRG 1 cut(s) 447
Aor13HI TCCGGA 1 cut(s) 20
AoxI GGCC 3 cut(s) 438, 644, 1785
ApeKI GCWGC 1 cut(s) 152
ApoI RAATTY 7 cut(s) 86, 97, 298, 488, 518, 620, 1204
Asp718I GGTACC 1 cut(s) 1415
AspLEI GCGC 1 cut(s) 1151
AspS9I GGNCC 2 cut(s) 439, 1564
AsuC2I CCSGG 1 cut(s) 1756
AsuHPI GGTGA 2 cut(s) 1502, 1825
AsuII TTCGAA 2 cut(s) 831, 1009
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 1 cut(s) 1564
BaeGI GKGCMC 1 cut(s) 1342
BalI TGGCCA 1 cut(s) 1787
BanI GGYRCC 2 cut(s) 1355, 1415
BauI CACGAG 2 cut(s) 696, 1245
BbsI GAAGAC 1 cut(s) 1018
BbvI GCAGC 1 cut(s) 164
BciT130I CCWGG 6 cut(s) 317, 669, 1401, 1413, 1572, 1640
BciVI GTATCC 2 cut(s) 249, 979
BclI TGATCA 1 cut(s) 67
BcnI CCSGG 1 cut(s) 1756
BcoDI GTCTC 3 cut(s) 133, 292, 567
BfaI CTAG 5 cut(s) 117, 443, 792, 903, 1386
BfmI CTRYAG 3 cut(s) 5, 150, 1320
BfuAI ACCTGC 1 cut(s) 597
BfuI GTATCC 2 cut(s) 249, 979
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
Bme1390I CCNGG 7 cut(s) 317, 669, 1401, 1413, 1572, 1640, 1756
Bme18I GGWCC 1 cut(s) 1564
BmeT110I CYCGRG 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 439, 1564
BmiI GGNNCC 4 cut(s) 1357, 1417, 1565, 1566
BmrFI CCNGG 7 cut(s) 317, 669, 1401, 1413, 1572, 1640, 1756
BmrI ACTGGG 1 cut(s) 1569
BmsI GCATC 2 cut(s) 1043, 1760
BmuI ACTGGG 1 cut(s) 1569
BpiI GAAGAC 1 cut(s) 1018
BplI GAGNNNNNCTC 2 cut(s) 244, 276
BpmI CTGGAG 2 cut(s) 1395, 1554
Bpu14I TTCGAA 2 cut(s) 831, 1009
BpuMI CCSGG 1 cut(s) 1756
BsaBI GATNNNNATC 2 cut(s) 548, 1916
BsaI GGTCTC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 972, 1399
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 1201, 1231
Bsc4I CCNNNNNNNGG 2 cut(s) 599, 1352
Bse1I ACTGG 7 cut(s) 342, 677, 774, 1128, 1451, 1564, 1693
Bse3DI GCAATG 3 cut(s) 423, 767, 1492
Bse8I GATNNNNATC 2 cut(s) 548, 1916
BseAI TCCGGA 1 cut(s) 20
BseBI CCWGG 6 cut(s) 317, 669, 1401, 1413, 1572, 1640
BseDI CCNNGG 2 cut(s) 972, 1399
BseGI GGATG 6 cut(s) 1031, 1058, 1290, 1723, 1751, 1764
BseJI GATNNNNATC 2 cut(s) 548, 1916
BseLI CCNNNNNNNGG 2 cut(s) 599, 1352
BseMI GCAATG 3 cut(s) 423, 767, 1492
BseMII CTCAG 4 cut(s) 84, 293, 503, 861
BseNI ACTGG 7 cut(s) 342, 677, 774, 1128, 1451, 1564, 1693
BseRI GAGGAG 2 cut(s) 1857, 1857
BseSI GKGCMC 1 cut(s) 1342
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 1581
BshFI GGCC 3 cut(s) 440, 646, 1787
BshNI GGYRCC 2 cut(s) 1355, 1415
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 2 cut(s) 21, 1755
BslFI GGGAC 2 cut(s) 1439, 1577
BslI CCNNNNNNNGG 2 cut(s) 599, 1352
BsmAI GTCTC 3 cut(s) 133, 292, 567
BsmFI GGGAC 2 cut(s) 1439, 1577
BsnI GGCC 3 cut(s) 440, 646, 1787
Bso31I GGTCTC 1 cut(s) 133
BsoBI CYCGRG 1 cut(s) 447
Bsp119I TTCGAA 2 cut(s) 831, 1009
Bsp1286I GDGCHC 1 cut(s) 1342
Bsp13I TCCGGA 1 cut(s) 20
Bsp143I GATC 2 cut(s) 67, 346
BspACI CCGC 3 cut(s) 37, 165, 900
BspANI GGCC 3 cut(s) 440, 646, 1787
BspCNI CTCAG 4 cut(s) 85, 292, 504, 862
BspEI TCCGGA 1 cut(s) 20
BspFNI CGCG 1 cut(s) 1581
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 4 cut(s) 1357, 1417, 1565, 1566
BspMAI CTGCAG 1 cut(s) 154
BspMI ACCTGC 1 cut(s) 597
BspQI GCTCTTC 1 cut(s) 417
BspT104I TTCGAA 2 cut(s) 831, 1009
BspT107I GGYRCC 2 cut(s) 1355, 1415
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 3 cut(s) 423, 767, 1492
BsrI ACTGG 7 cut(s) 342, 677, 774, 1128, 1451, 1564, 1693
BssECI CCNNGG 2 cut(s) 972, 1399
BssMI GATC 2 cut(s) 67, 346
BssSI CACGAG 2 cut(s) 696, 1245
BssT1I CCWWGG 1 cut(s) 972
Bst2BI CACGAG 2 cut(s) 696, 1245
Bst2UI CCWGG 6 cut(s) 317, 669, 1401, 1413, 1572, 1640
Bst4CI ACNGT 2 cut(s) 56, 1909
Bst6I CTCTTC 2 cut(s) 19, 417
BstBI TTCGAA 2 cut(s) 831, 1009
BstC8I GCNNGC 1 cut(s) 325
BstDEI CTNAG 7 cut(s) 93, 279, 432, 494, 512, 870, 1059
BstF5I GGATG 6 cut(s) 1031, 1058, 1290, 1723, 1751, 1764
BstFNI CGCG 1 cut(s) 1581
BstHHI GCGC 1 cut(s) 1151
BstKTI GATC 2 cut(s) 70, 349
BstMAI GTCTC 3 cut(s) 133, 292, 567
BstMBI GATC 2 cut(s) 67, 346
BstMWI GCNNNNNNNGC 5 cut(s) 413, 1148, 1553, 1706, 1748
BstNI CCWGG 6 cut(s) 317, 669, 1401, 1413, 1572, 1640
BstNSI RCATGY 2 cut(s) 269, 1082
BstSCI CCNGG 7 cut(s) 315, 667, 1399, 1411, 1570, 1638, 1754
BstSFI CTRYAG 3 cut(s) 5, 150, 1320
BstSLI GKGCMC 1 cut(s) 1342
BstUI CGCG 1 cut(s) 1581
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 1 cut(s) 1018
BsuI GTATCC 2 cut(s) 249, 979
BsuRI GGCC 3 cut(s) 440, 646, 1787
BtsCI GGATG 6 cut(s) 1031, 1058, 1290, 1723, 1751, 1764
BtsI GCAGTG 1 cut(s) 470
BtsIMutI CAGTG 5 cut(s) 349, 403, 470, 1121, 1318
BveI ACCTGC 1 cut(s) 597
Cac8I GCNNGC 1 cut(s) 325
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 1151
Cfr13I GGNCC 2 cut(s) 439, 1564
Csp6I GTAC 3 cut(s) 534, 1416, 1669
CspCI CAANNNNNGTGG 2 cut(s) 786, 821
CviQI GTAC 3 cut(s) 534, 1416, 1669
DdeI CTNAG 7 cut(s) 93, 279, 432, 494, 512, 870, 1059
DpnI GATC 2 cut(s) 69, 348
DpnII GATC 2 cut(s) 67, 346
DraI TTTAAA 1 cut(s) 1203
EaeI YGGCCR 1 cut(s) 1785
Eam1104I CTCTTC 2 cut(s) 19, 417
EarI CTCTTC 2 cut(s) 19, 417
Eco130I CCWWGG 1 cut(s) 972
Eco31I GGTCTC 1 cut(s) 133
Eco32I GATATC 2 cut(s) 502, 1309
Eco47I GGWCC 1 cut(s) 1564
Eco88I CYCGRG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 1564
EcoRII CCWGG 6 cut(s) 315, 667, 1399, 1411, 1570, 1638
EcoRV GATATC 2 cut(s) 502, 1309
EcoT14I CCWWGG 1 cut(s) 972
ErhI CCWWGG 1 cut(s) 972
FaqI GGGAC 2 cut(s) 1439, 1577
FauI CCCGC 2 cut(s) 44, 907
FauNDI CATATG 1 cut(s) 1067
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 6 cut(s) 1038, 1065, 1297, 1730, 1738, 1751
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 5 cut(s) 117, 443, 792, 903, 1386
GlaI GCGC 1 cut(s) 1150
GluI GCNGC 1 cut(s) 153
GsuI CTGGAG 2 cut(s) 1395, 1554
HaeIII GGCC 3 cut(s) 440, 646, 1787
HapII CCGG 2 cut(s) 21, 1755
HhaI GCGC 1 cut(s) 1151
Hin6I GCGC 1 cut(s) 1149
HinP1I GCGC 1 cut(s) 1149
HincII GTYRAC 2 cut(s) 730, 1815
HindII GTYRAC 2 cut(s) 730, 1815
HindIII AAGCTT 2 cut(s) 1261, 1271
HpaII CCGG 2 cut(s) 21, 1755
HphI GGTGA 2 cut(s) 1502, 1825
Hpy166II GTNNAC 6 cut(s) 247, 730, 932, 1105, 1678, 1815
Hpy188I TCNGA 9 cut(s) 94, 412, 513, 586, 602, 619, 688, 1279, 1963
Hpy8I GTNNAC 6 cut(s) 247, 730, 932, 1105, 1678, 1815
HpyAV CCTTC 4 cut(s) 827, 1609, 1778, 1832
HpyCH4III ACNGT 2 cut(s) 56, 1909
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 7 cut(s) 152, 269, 323, 856, 920, 1142, 1828
HpyF10VI GCNNNNNNNGC 5 cut(s) 413, 1148, 1553, 1706, 1748
HpyF3I CTNAG 7 cut(s) 93, 279, 432, 494, 512, 870, 1059
HpySE526I ACGT 1 cut(s) 536
HspAI GCGC 1 cut(s) 1149
KflI GGGWCCC 1 cut(s) 1564
Kpn2I TCCGGA 1 cut(s) 20
KpnI GGTACC 1 cut(s) 1419
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 2 cut(s) 67, 346
LguI GCTCTTC 1 cut(s) 417
LmnI GCTCC 2 cut(s) 1075, 1844
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 2 cut(s) 1043, 1760
MaeI CTAG 5 cut(s) 117, 443, 792, 903, 1386
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 5 cut(s) 361, 467, 1372, 1609, 1646
MalI GATC 2 cut(s) 69, 348
MboI GATC 2 cut(s) 67, 346
MboII GAAGA 8 cut(s) 36, 116, 434, 684, 1023, 1266, 1373, 1439
MfeI CAATTG 1 cut(s) 740
MhlI GDGCHC 1 cut(s) 1342
MlsI TGGCCA 1 cut(s) 1787
MluNI TGGCCA 1 cut(s) 1787
MlyI GAGTC 3 cut(s) 569, 1649, 1810
Mox20I TGGCCA 1 cut(s) 1787
MroI TCCGGA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 1787
MseI TTAA 6 cut(s) 194, 609, 1031, 1116, 1202, 1391
MslI CAYNNNNRTG 3 cut(s) 557, 1024, 1070
Msp20I TGGCCA 1 cut(s) 1787
MspA1I CMGCKG 2 cut(s) 869, 937
MspI CCGG 2 cut(s) 21, 1755
MspR9I CCNGG 7 cut(s) 317, 669, 1401, 1413, 1572, 1640, 1756
MunI CAATTG 1 cut(s) 740
MvaI CCWGG 6 cut(s) 317, 669, 1401, 1413, 1572, 1640
MvnI CGCG 1 cut(s) 1581
MwoI GCNNNNNNNGC 5 cut(s) 413, 1148, 1553, 1706, 1748
NciI CCSGG 1 cut(s) 1756
NdeI CATATG 1 cut(s) 1067
NdeII GATC 2 cut(s) 67, 346
NlaIV GGNNCC 4 cut(s) 1357, 1417, 1565, 1566
NmuCI GTSAC 2 cut(s) 467, 1372
NspI RCATGY 2 cut(s) 269, 1082
NspV TTCGAA 2 cut(s) 831, 1009
PaeR7I CTCGAG 1 cut(s) 447
PagI TCATGA 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 417
PfeI GAWTC 8 cut(s) 597, 689, 828, 1493, 1500, 1796, 1912, 1958
PflFI GACNNNGTC 1 cut(s) 710
PfoI TCCNGGA 2 cut(s) 1570, 1638
PkrI GCNGC 1 cut(s) 154
PleI GAGTC 3 cut(s) 569, 1649, 1810
PpsI GAGTC 3 cut(s) 569, 1649, 1810
PpuMI RGGWCCY 1 cut(s) 1564
Psp5II RGGWCCY 1 cut(s) 1564
Psp6I CCWGG 6 cut(s) 315, 667, 1399, 1411, 1570, 1638
PspGI CCWGG 6 cut(s) 315, 667, 1399, 1411, 1570, 1638
PspN4I GGNNCC 4 cut(s) 1357, 1417, 1565, 1566
PspPI GGNCC 2 cut(s) 439, 1564
PspPPI RGGWCCY 1 cut(s) 1564
PstI CTGCAG 1 cut(s) 154
PsyI GACNNNGTC 1 cut(s) 710
PvuII CAGCTG 2 cut(s) 869, 937
RsaI GTAC 3 cut(s) 535, 1417, 1670
RsaNI GTAC 3 cut(s) 534, 1416, 1669
RseI CAYNNNNRTG 3 cut(s) 557, 1024, 1070
SapI GCTCTTC 1 cut(s) 417
SaqAI TTAA 6 cut(s) 194, 609, 1031, 1116, 1202, 1391
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 2 cut(s) 67, 346
Sau96I GGNCC 2 cut(s) 439, 1564
SchI GAGTC 3 cut(s) 569, 1649, 1810
ScrFI CCNGG 7 cut(s) 317, 669, 1401, 1413, 1572, 1640, 1756
SduI GDGCHC 1 cut(s) 1342
SfaNI GCATC 2 cut(s) 1043, 1760
SfcI CTRYAG 3 cut(s) 5, 150, 1320
Sfr274I CTCGAG 1 cut(s) 447
SfuI TTCGAA 2 cut(s) 831, 1009
SinI GGWCC 1 cut(s) 1564
SlaI CTCGAG 1 cut(s) 447
SmiMI CAYNNNNRTG 3 cut(s) 557, 1024, 1070
SmlI CTYRAG 1 cut(s) 447
SmoI CTYRAG 1 cut(s) 447
SsiI CCGC 3 cut(s) 37, 165, 900
SspI AATATT 3 cut(s) 46, 331, 784
SspMI CTAG 5 cut(s) 117, 443, 792, 903, 1386
StyD4I CCNGG 7 cut(s) 315, 667, 1399, 1411, 1570, 1638, 1754
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 2 cut(s) 56, 1909
TaiI ACGT 1 cut(s) 539
TaqI TCGA 9 cut(s) 448, 504, 626, 653, 831, 1009, 1177, 1498, 1586
TfiI GAWTC 8 cut(s) 597, 689, 828, 1493, 1500, 1796, 1912, 1958
Tru1I TTAA 6 cut(s) 194, 609, 1031, 1116, 1202, 1391
Tru9I TTAA 6 cut(s) 194, 609, 1031, 1116, 1202, 1391
TscAI CASTG 5 cut(s) 349, 403, 470, 1128, 1318
TseFI GTSAC 2 cut(s) 467, 1372
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 467, 1372
TspDTI ATGAA 6 cut(s) 98, 404, 734, 866, 1539, 1809
TspGWI ACGGA 1 cut(s) 1865
TspRI CASTG 5 cut(s) 349, 403, 470, 1128, 1318
Tth111I GACNNNGTC 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 1564
XapI RAATTY 7 cut(s) 86, 97, 298, 488, 518, 620, 1204
XbaI TCTAGA 1 cut(s) 791
XceI RCATGY 2 cut(s) 269, 1082
XhoI CTCGAG 1 cut(s) 447
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 5 cut(s) 117, 443, 792, 903, 1386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.