Rw5G023850

zipper protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016791
Physical Location & Seq
Forward (+)
33731196 .. 33734931
3736 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023850.1

Sequence Viewer

Length: 1743 bp
ATGGACAGCAGTATTTCACAAATTGAGGTAGACATGAGGATACCTCAACATGCAACACCATCTCAGCAAGTCTCATTGGAGAATTTGGGGTCAAATGTCCAGGTTGCAAGCTATATTTCCAGAGCCAGTGATCTACTCATGGCAGTAACTTCAAGTGCTGAAATCAACAGAATGAAAATCAGTGAGTTAGCTTCGGAAGCAATGGAAGAGCTAACTAAATTGGCCCTAGCTCGAGAACCTCTATGGCAGTGTGACATTGAGAGCAATACTGAATTTCTTAGTGATATCGAGTATCTGAGGGAATTTGGACACATAAGTACGTCATTGATAGAAATCATTAGAATGGTTGAGGTGGGAGACTCTCAATCTGACCTGCCAAGATTCGGAAGCATTAACAATTCTGAATTTTCGATGGAAAGTGAATATAGGCCAATATCGACAACAAATCAACCTGGAAAACCAGTTAGTTCGGAATCTTCACGAGCTATTGACTATGTCAAAATGAAAGCAGTCAACATTGTTCAATTGCTAATGGATTTGAAGCAATGGCTACTGGTGTTTCCTAATATTGTATCTAGAGCAAAAGTAGTGGGAGTATTATCATCAACAAGATTCGAAGGCAACCATGATGGAACTCTGCAAGTGATGACAGCTGAGTTTCATGCCCCAACACCACTTATTCCCGCTAGAGAAAGTTATTTTGCAAGGTATAGTAAACAGCTGGACAGGGGAATGTGGGTAGTTGTTGATGTATCCTTGGAAAAATTGTTTCAGCTTCCATCAACAAACTTTCGAAGACAACCATCAGGATGTTTAATTCAGGAAATGTCAAATGGATGCTCTAAGGTCATATGGGTGGAGCATGTGGAAGTGGATAATAGAATGGTTCACAATATGTTTAAGCCACTGGTCAGTTCTGGTTTTGCATTTAGCGCAAAGCGTTGGGTTTCCACTTTAGTTCGACAATGTGAATGGTTGGCAACTTTAAATTCTACAAGCCCTCCTACATCAGATTGTATGTTCGTATCTCGAGCTGGAAGAAAAAGCTTGTTGAAGCTTTCGGAGAGGATGATGAGAAGTTTTGTTGTTGATATTAGTGCTTCTACAGAAAACAAATGGAGGGCACTACCTGTATTTGGTGCCGAAGATGTAAAGGTCACAACTAAAACTAGCTTAAATGACCCTGGAAAGCCTCCAGGTACCACCTTATTATTTGCTACTTCTGTCCATCTTCCAGTCCCAAGAACTCAAATATACAATCTGCTTCGCAATGGTGAATCTCGAATCATGTGGGATATGATGTCGTATGAAAAAGATACAAGAGAAATTGCTTATATCGCAACTGGGGACCCTCCAGGAAATCGCGTTTCGATAATTGAAGTAGATAATGAGATTGAAGTTTTCTACTTCCAGGAGAGTTACACAGACTCAAATGGCTCGTACATAGTTTACGCACCGATTGACCAGTTCGCCATGTCTGCTCTCTTGGATGGCTCAAACCCAGACCATGTTGCTATTCTCGCATCCGGGTTTTCCATCCTTCCTGATAGACCAATGGCCAATGATGAATCTGGTGGAAGTCTGTTGACTCTTGCGTTGCACATCACCGAAGGAGGAGCAACGGAGGAATATATCCCTCCTCAAACTTTTCAAAATATACTCAACATTATTGCTGGAACTGTGATTCAAATCACAGAAGTATTATCAAATGATGGAGAAAATAGTGTTGAATCAGAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

580

Amino Acids

64.62

Weight (kDa)

4.91

Isoelectric Point (pI)

48.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 139 - 324 8.5e-34 START domain
PDF2_C PF25797 344 - 568 6.3e-59 PDF2-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016791)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g22020 FvH4_3g22021
malus_domestica MD11G1223900.v1.1
prunus_persica Prupe.4G205900_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0037891
rosa_laevigata RLG00000033820
rosa_multiflora Rmu_sc0005416.1_g000025
rosa_roxburghii Rroxscaffold_1G00043030
rosa_rugosa Rorug05G0168500
rosa_samantha Rh5AG257100 Rh5BG259700 Rh5CG292700 Rh5DG269600
rosa_wichuraiana Rw5G023850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 381
Acc65I GGTACC 1 cut(s) 1199
AccB1I GGYRCC 2 cut(s) 1139, 1199
AccI GTMKAC 1 cut(s) 30
AccII CGCG 1 cut(s) 1365
AciI CCGC 1 cut(s) 684
AcoI YGGCCR 1 cut(s) 1557
AcsI RAATTY 5 cut(s) 82, 272, 302, 404, 988
AfaI GTAC 3 cut(s) 319, 1201, 1442
AfiI CCNNNNNNNGG 2 cut(s) 383, 1136
AgsI TTSAA 9 cut(s) 153, 524, 541, 1054, 1379, 1397, 1652, 1688, 1730
AjnI CCWGG 6 cut(s) 99, 451, 1183, 1195, 1354, 1410
Alw26I GTCTC 2 cut(s) 76, 351
Ama87I CYCGRG 2 cut(s) 231, 1029
AoxI GGCC 3 cut(s) 222, 428, 1557
ApoI RAATTY 5 cut(s) 82, 272, 302, 404, 988
Asp718I GGTACC 1 cut(s) 1199
AspLEI GCGC 1 cut(s) 935
AspS9I GGNCC 2 cut(s) 223, 1348
AsuC2I CCSGG 1 cut(s) 1528
AsuHPI GGTGA 2 cut(s) 1286, 1597
AsuII TTCGAA 2 cut(s) 615, 793
AvaI CYCGRG 2 cut(s) 231, 1029
AvaII GGWCC 1 cut(s) 1348
BaeGI GKGCMC 1 cut(s) 1126
BalI TGGCCA 1 cut(s) 1559
BanI GGYRCC 2 cut(s) 1139, 1199
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 802
BccI CCATC 9 cut(s) 67, 406, 623, 787, 811, 1236, 1484, 1544, 1706
BciT130I CCWGG 6 cut(s) 101, 453, 1185, 1197, 1356, 1412
BciVI GTATCC 2 cut(s) 33, 763
BcnI CCSGG 1 cut(s) 1528
BcoDI GTCTC 2 cut(s) 76, 351
BfaI CTAG 4 cut(s) 227, 576, 687, 1170
BfmI CTRYAG 1 cut(s) 1104
BfuAI ACCTGC 1 cut(s) 381
BfuI GTATCC 2 cut(s) 33, 763
Bme1390I CCNGG 7 cut(s) 101, 453, 1185, 1197, 1356, 1412, 1528
Bme18I GGWCC 1 cut(s) 1348
BmeT110I CYCGRG 2 cut(s) 231, 1029
BmgT120I GGNCC 2 cut(s) 223, 1348
BmiI GGNNCC 4 cut(s) 1141, 1201, 1349, 1350
BmrFI CCNGG 7 cut(s) 101, 453, 1185, 1197, 1356, 1412, 1528
BmrI ACTGGG 1 cut(s) 1353
BmsI GCATC 2 cut(s) 827, 1532
BmuI ACTGGG 1 cut(s) 1353
BpiI GAAGAC 1 cut(s) 802
BplI GAGNNNNNCTC 2 cut(s) 28, 60
BpmI CTGGAG 2 cut(s) 1179, 1338
Bpu14I TTCGAA 2 cut(s) 615, 793
BpuMI CCSGG 1 cut(s) 1528
BsaBI GATNNNNATC 2 cut(s) 332, 1688
BsaJI CCNNGG 2 cut(s) 756, 1183
BsaXI ACNNNNNCTCC 2 cut(s) 985, 1015
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 1136
Bse1I ACTGG 7 cut(s) 126, 461, 558, 912, 1235, 1348, 1465
Bse3DI GCAATG 3 cut(s) 207, 551, 1276
Bse8I GATNNNNATC 2 cut(s) 332, 1688
BseBI CCWGG 6 cut(s) 101, 453, 1185, 1197, 1356, 1412
BseDI CCNNGG 2 cut(s) 756, 1183
BseGI GGATG 6 cut(s) 815, 842, 1074, 1495, 1523, 1536
BseJI GATNNNNATC 2 cut(s) 332, 1688
BseLI CCNNNNNNNGG 2 cut(s) 383, 1136
BseMI GCAATG 3 cut(s) 207, 551, 1276
BseMII CTCAG 3 cut(s) 77, 287, 645
BseNI ACTGG 7 cut(s) 126, 461, 558, 912, 1235, 1348, 1465
BseRI GAGGAG 2 cut(s) 1629, 1629
BseSI GKGCMC 1 cut(s) 1126
Bsh1236I CGCG 1 cut(s) 1365
BshFI GGCC 3 cut(s) 224, 430, 1559
BshNI GGYRCC 2 cut(s) 1139, 1199
BsiHKCI CYCGRG 2 cut(s) 231, 1029
BsiSI CCGG 1 cut(s) 1527
BslFI GGGAC 2 cut(s) 1223, 1361
BslI CCNNNNNNNGG 2 cut(s) 383, 1136
BsmAI GTCTC 2 cut(s) 76, 351
BsmFI GGGAC 2 cut(s) 1223, 1361
BsnI GGCC 3 cut(s) 224, 430, 1559
BsoBI CYCGRG 2 cut(s) 231, 1029
Bsp119I TTCGAA 2 cut(s) 615, 793
Bsp1286I GDGCHC 1 cut(s) 1126
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 1 cut(s) 684
BspANI GGCC 3 cut(s) 224, 430, 1559
BspCNI CTCAG 3 cut(s) 76, 288, 646
BspFNI CGCG 1 cut(s) 1365
BspLI GGNNCC 4 cut(s) 1141, 1201, 1349, 1350
BspMI ACCTGC 1 cut(s) 381
BspQI GCTCTTC 1 cut(s) 201
BspT104I TTCGAA 2 cut(s) 615, 793
BspT107I GGYRCC 2 cut(s) 1139, 1199
BsrDI GCAATG 3 cut(s) 207, 551, 1276
BsrI ACTGG 7 cut(s) 126, 461, 558, 912, 1235, 1348, 1465
BssECI CCNNGG 2 cut(s) 756, 1183
BssMI GATC 1 cut(s) 130
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 756
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 6 cut(s) 101, 453, 1185, 1197, 1356, 1412
Bst4CI ACNGT 1 cut(s) 1681
Bst6I CTCTTC 1 cut(s) 201
BstBI TTCGAA 2 cut(s) 615, 793
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 5 cut(s) 63, 278, 296, 654, 843
BstF5I GGATG 6 cut(s) 815, 842, 1074, 1495, 1523, 1536
BstFNI CGCG 1 cut(s) 1365
BstHHI GCGC 1 cut(s) 935
BstKTI GATC 1 cut(s) 133
BstMAI GTCTC 2 cut(s) 76, 351
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 197, 932, 1337, 1478, 1520
BstNI CCWGG 6 cut(s) 101, 453, 1185, 1197, 1356, 1412
BstNSI RCATGY 2 cut(s) 53, 866
BstSCI CCNGG 7 cut(s) 99, 451, 1183, 1195, 1354, 1410, 1526
BstSFI CTRYAG 1 cut(s) 1104
BstSLI GKGCMC 1 cut(s) 1126
BstUI CGCG 1 cut(s) 1365
BstV2I GAAGAC 1 cut(s) 802
BsuI GTATCC 2 cut(s) 33, 763
BsuRI GGCC 3 cut(s) 224, 430, 1559
BtsCI GGATG 6 cut(s) 815, 842, 1074, 1495, 1523, 1536
BtsI GCAGTG 1 cut(s) 254
BtsIMutI CAGTG 4 cut(s) 133, 187, 254, 905
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 1 cut(s) 935
Cfr13I GGNCC 2 cut(s) 223, 1348
Csp6I GTAC 3 cut(s) 318, 1200, 1441
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 9 cut(s) 34, 50, 139, 626, 662, 863, 1288, 1474, 1508
CviQI GTAC 3 cut(s) 318, 1200, 1441
DdeI CTNAG 5 cut(s) 63, 278, 296, 654, 843
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
DraI TTTAAA 1 cut(s) 987
EaeI YGGCCR 1 cut(s) 1557
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 756
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 1348
Eco88I CYCGRG 2 cut(s) 231, 1029
EcoO109I RGGNCCY 1 cut(s) 1348
EcoRII CCWGG 6 cut(s) 99, 451, 1183, 1195, 1354, 1410
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 756
ErhI CCWWGG 1 cut(s) 756
FaeI CATG 9 cut(s) 37, 53, 142, 629, 665, 866, 1291, 1477, 1511
FaqI GGGAC 2 cut(s) 1223, 1361
FatI CATG 9 cut(s) 33, 49, 138, 625, 661, 862, 1287, 1473, 1507
FauI CCCGC 1 cut(s) 691
FauNDI CATATG 1 cut(s) 851
FblI GTMKAC 1 cut(s) 30
FokI GGATG 6 cut(s) 822, 849, 1081, 1502, 1510, 1523
FspBI CTAG 4 cut(s) 227, 576, 687, 1170
GlaI GCGC 1 cut(s) 934
GsuI CTGGAG 2 cut(s) 1179, 1338
HaeIII GGCC 3 cut(s) 224, 430, 1559
HapII CCGG 1 cut(s) 1527
HhaI GCGC 1 cut(s) 935
Hin1II CATG 9 cut(s) 37, 53, 142, 629, 665, 866, 1291, 1477, 1511
Hin6I GCGC 1 cut(s) 933
HinP1I GCGC 1 cut(s) 933
HincII GTYRAC 2 cut(s) 514, 1587
HindII GTYRAC 2 cut(s) 514, 1587
HindIII AAGCTT 2 cut(s) 1045, 1055
HpaII CCGG 1 cut(s) 1527
HphI GGTGA 2 cut(s) 1286, 1597
Hpy166II GTNNAC 6 cut(s) 31, 514, 716, 889, 1450, 1587
Hpy188I TCNGA 9 cut(s) 196, 297, 370, 386, 403, 472, 1012, 1063, 1735
Hpy188III TCNNGA 9 cut(s) 120, 233, 480, 576, 807, 821, 1029, 1281, 1544
Hpy8I GTNNAC 6 cut(s) 31, 514, 716, 889, 1450, 1587
HpyAV CCTTC 3 cut(s) 611, 1550, 1604
HpyCH4III ACNGT 1 cut(s) 1681
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 6 cut(s) 53, 107, 640, 704, 926, 1600
HpyF10VI GCNNNNNNNGC 5 cut(s) 197, 932, 1337, 1478, 1520
HpyF3I CTNAG 5 cut(s) 63, 278, 296, 654, 843
HpySE526I ACGT 1 cut(s) 320
Hsp92II CATG 9 cut(s) 37, 53, 142, 629, 665, 866, 1291, 1477, 1511
HspAI GCGC 1 cut(s) 933
KflI GGGWCCC 1 cut(s) 1348
KpnI GGTACC 1 cut(s) 1203
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 2 cut(s) 859, 1616
LweI GCATC 2 cut(s) 827, 1532
MaeI CTAG 4 cut(s) 227, 576, 687, 1170
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 4 cut(s) 145, 251, 1156, 1418
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 6 cut(s) 218, 468, 807, 1050, 1157, 1223
MfeI CAATTG 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 1126
MlsI TGGCCA 1 cut(s) 1559
MluNI TGGCCA 1 cut(s) 1559
MlyI GAGTC 3 cut(s) 353, 1421, 1582
Mox20I TGGCCA 1 cut(s) 1559
MscI TGGCCA 1 cut(s) 1559
MseI TTAA 5 cut(s) 393, 815, 900, 986, 1175
MslI CAYNNNNRTG 3 cut(s) 341, 808, 854
Msp20I TGGCCA 1 cut(s) 1559
MspA1I CMGCKG 2 cut(s) 653, 721
MspI CCGG 1 cut(s) 1527
MspR9I CCNGG 7 cut(s) 101, 453, 1185, 1197, 1356, 1412, 1528
MunI CAATTG 1 cut(s) 524
MvaI CCWGG 6 cut(s) 101, 453, 1185, 1197, 1356, 1412
MvnI CGCG 1 cut(s) 1365
MwoI GCNNNNNNNGC 5 cut(s) 197, 932, 1337, 1478, 1520
NciI CCSGG 1 cut(s) 1528
NdeI CATATG 1 cut(s) 851
NdeII GATC 1 cut(s) 130
NlaIII CATG 9 cut(s) 37, 53, 142, 629, 665, 866, 1291, 1477, 1511
NlaIV GGNNCC 4 cut(s) 1141, 1201, 1349, 1350
NmuCI GTSAC 2 cut(s) 251, 1156
NspI RCATGY 2 cut(s) 53, 866
NspV TTCGAA 2 cut(s) 615, 793
PaeR7I CTCGAG 2 cut(s) 231, 1029
PciSI GCTCTTC 1 cut(s) 201
PfeI GAWTC 8 cut(s) 381, 473, 612, 1277, 1284, 1568, 1684, 1730
PflFI GACNNNGTC 1 cut(s) 494
PfoI TCCNGGA 2 cut(s) 1354, 1410
PleI GAGTC 3 cut(s) 353, 1421, 1582
PpsI GAGTC 3 cut(s) 353, 1421, 1582
PpuMI RGGWCCY 1 cut(s) 1348
Psp5II RGGWCCY 1 cut(s) 1348
Psp6I CCWGG 6 cut(s) 99, 451, 1183, 1195, 1354, 1410
PspGI CCWGG 6 cut(s) 99, 451, 1183, 1195, 1354, 1410
PspN4I GGNNCC 4 cut(s) 1141, 1201, 1349, 1350
PspPI GGNCC 2 cut(s) 223, 1348
PspPPI RGGWCCY 1 cut(s) 1348
PsyI GACNNNGTC 1 cut(s) 494
PvuII CAGCTG 2 cut(s) 653, 721
RsaI GTAC 3 cut(s) 319, 1201, 1442
RsaNI GTAC 3 cut(s) 318, 1200, 1441
RseI CAYNNNNRTG 3 cut(s) 341, 808, 854
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 5 cut(s) 393, 815, 900, 986, 1175
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 2 cut(s) 223, 1348
SchI GAGTC 3 cut(s) 353, 1421, 1582
ScrFI CCNGG 7 cut(s) 101, 453, 1185, 1197, 1356, 1412, 1528
SduI GDGCHC 1 cut(s) 1126
SfaNI GCATC 2 cut(s) 827, 1532
SfcI CTRYAG 1 cut(s) 1104
Sfr274I CTCGAG 2 cut(s) 231, 1029
SfuI TTCGAA 2 cut(s) 615, 793
SinI GGWCC 1 cut(s) 1348
SlaI CTCGAG 2 cut(s) 231, 1029
SmiMI CAYNNNNRTG 3 cut(s) 341, 808, 854
SmlI CTYRAG 2 cut(s) 231, 1029
SmoI CTYRAG 2 cut(s) 231, 1029
SsiI CCGC 1 cut(s) 684
SspI AATATT 1 cut(s) 568
SspMI CTAG 4 cut(s) 227, 576, 687, 1170
StyD4I CCNGG 7 cut(s) 99, 451, 1183, 1195, 1354, 1410, 1526
StyI CCWWGG 1 cut(s) 756
TaaI ACNGT 1 cut(s) 1681
TaiI ACGT 1 cut(s) 323
TfiI GAWTC 8 cut(s) 381, 473, 612, 1277, 1284, 1568, 1684, 1730
Tru1I TTAA 5 cut(s) 393, 815, 900, 986, 1175
Tru9I TTAA 5 cut(s) 393, 815, 900, 986, 1175
TscAI CASTG 4 cut(s) 133, 187, 254, 912
TseFI GTSAC 2 cut(s) 251, 1156
Tsp45I GTSAC 2 cut(s) 251, 1156
TspDTI ATGAA 5 cut(s) 188, 518, 650, 1323, 1581
TspGWI ACGGA 1 cut(s) 1637
TspRI CASTG 4 cut(s) 133, 187, 254, 912
Tth111I GACNNNGTC 1 cut(s) 494
VpaK11BI GGWCC 1 cut(s) 1348
XapI RAATTY 5 cut(s) 82, 272, 302, 404, 988
XbaI TCTAGA 1 cut(s) 575
XceI RCATGY 2 cut(s) 53, 866
XhoI CTCGAG 2 cut(s) 231, 1029
XmiI GTMKAC 1 cut(s) 30
XspI CTAG 4 cut(s) 227, 576, 687, 1170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.