FvH4_3g24700

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012758
Physical Location & Seq
Reverse (-)
17813889 .. 17815162
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24700.t1

Sequence Viewer

Length: 522 bp
ATGGCAAAGATCTCAGATCCTCTTGTTGTTGGAAGAGTCATTGGAGATGTTATTGATTGTTTCACACCCAGTGTTAAAATGACAGTGACTTACAACTCCAGCAAGAAGGTGTATAATGGGCATGAGCTATATCCTTCCTCTGTAACCGTCAAGCCTAAGGTTGAAGTTCATGGAGGCGATATGAGATCTTTCTTTACACTGGTCATGACTGACCCAGATGTTCCAGGTCCTAGTGATCCTTACCTGAAGGAGCACTTGCACTGGATAGTCACGGACATCCCAGGAACAACTGACAACACATTTGGAAAGGAGGTGGTGAAATATGAAATGCCGAGGCCAAACATAGGGATCCACAGGTTTGTGTTCCTTTTGTTCAAACAGAAAGGTAGGCAGACAGTGATTCCTCCTCCTTCGAAGGACCATTTCGACAGTCGAAAGTTTGCAGAAACAAACGAGTTCGGGCTTCCGGTGGCTGCTGTCTTCTTCAATGCCCAGAGGGAAACTGCTGCAAGAAGACGCTAA

Protein Analysis

174

Amino Acids

19.52

Weight (kDa)

9.19

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 50 - 148 1.1e-11 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27550
fragaria_vesca FvH4_3g24700
malus_domestica MD03G1143000.v1.1 MD11G1163500.v1.1
prunus_persica Prupe.6G128400_v2.0.a1 Prupe.6G128400_v2.0.a1
pyrus_communis pycom03g09760
rosa_chinensis RchiOBHm_Chr5g0044251
rosa_laevigata RLG00000034260
rosa_multiflora Rmu_sc0000776.1_g000051
rosa_roxburghii Rroxscaffold_1G00036720
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297200 Rh5BG304600 Rh5CG331500 Rh5DG313500
rosa_wichuraiana Rw0G015760 Rw5G027520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 11, 230, 343, 356
AcuI CTGAAG 1 cut(s) 266
AdeI CACNNNGTG 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 344
AgsI TTSAA 3 cut(s) 164, 376, 487
AjnI CCWGG 2 cut(s) 223, 280
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 4 cut(s) 11, 230, 343, 356
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 473, 506
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspS9I GGNCC 2 cut(s) 227, 418
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 2 cut(s) 227, 418
AxyI CCTNAGG 1 cut(s) 156
BamHI GGATCC 1 cut(s) 348
BbsI GAAGAC 1 cut(s) 472
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 460, 493
BciT130I CCWGG 2 cut(s) 225, 282
BfaI CTAG 1 cut(s) 231
BglII AGATCT 2 cut(s) 9, 185
BisI GCNGC 2 cut(s) 474, 507
BlsI GCNGC 2 cut(s) 475, 508
Bme1390I CCNGG 2 cut(s) 225, 282
Bme18I GGWCC 2 cut(s) 227, 418
BmgT120I GGNCC 2 cut(s) 227, 418
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 225, 282
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
BpiI GAAGAC 1 cut(s) 472
BpmI CTGGAG 1 cut(s) 82
Bpu14I TTCGAA 1 cut(s) 413
BsaJI CCNNGG 2 cut(s) 280, 332
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 344
Bse1I ACTGG 3 cut(s) 69, 204, 266
Bse21I CCTNAGG 1 cut(s) 156
BseBI CCWGG 2 cut(s) 225, 282
BseDI CCNNGG 2 cut(s) 280, 332
BseGI GGATG 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 3 cut(s) 69, 204, 266
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 2 cut(s) 460, 493
BshFI GGCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 344
BsnI GGCC 1 cut(s) 337
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 5 cut(s) 9, 16, 185, 235, 348
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 26
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 4 cut(s) 11, 230, 343, 356
BspT104I TTCGAA 1 cut(s) 413
BsrI ACTGG 3 cut(s) 69, 204, 266
BssECI CCNNGG 2 cut(s) 280, 332
BssMI GATC 5 cut(s) 9, 16, 185, 235, 348
Bst2UI CCWGG 2 cut(s) 225, 282
Bst4CI ACNGT 4 cut(s) 85, 148, 397, 431
Bst6I CTCTTC 1 cut(s) 28
BstBI TTCGAA 1 cut(s) 413
BstDEI CTNAG 2 cut(s) 13, 156
BstF5I GGATG 1 cut(s) 276
BstKTI GATC 5 cut(s) 12, 19, 188, 238, 351
BstMBI GATC 5 cut(s) 9, 16, 185, 235, 348
BstNI CCWGG 2 cut(s) 225, 282
BstSCI CCNGG 2 cut(s) 223, 280
BstV1I GCAGC 2 cut(s) 460, 493
BstV2I GAAGAC 1 cut(s) 472
BstX2I RGATCY 4 cut(s) 9, 16, 185, 348
BstYI RGATCY 4 cut(s) 9, 16, 185, 348
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 5 cut(s) 76, 90, 197, 259, 402
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 227, 418
CviAII CATG 3 cut(s) 122, 170, 205
CviJI RGCY 5 cut(s) 127, 154, 337, 463, 473
CviKI_1 RGCY 5 cut(s) 127, 154, 337, 463, 473
DdeI CTNAG 2 cut(s) 13, 156
DpnI GATC 5 cut(s) 11, 18, 187, 237, 350
DpnII GATC 5 cut(s) 9, 16, 185, 235, 348
DraIII CACNNNGTG 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco47I GGWCC 2 cut(s) 227, 418
Eco57I CTGAAG 1 cut(s) 266
Eco81I CCTNAGG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 223, 280
FaeI CATG 3 cut(s) 125, 173, 208
FaiI YATR 8 cut(s) 114, 123, 130, 171, 182, 206, 324, 344
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 3 cut(s) 121, 169, 204
Fnu4HI GCNGC 2 cut(s) 474, 507
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 474, 507
FspBI CTAG 1 cut(s) 231
GluI GCNGC 2 cut(s) 474, 507
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 467
Hin1II CATG 3 cut(s) 125, 173, 208
HinfI GANTC 2 cut(s) 36, 400
HpaII CCGG 1 cut(s) 467
HphI GGTGA 1 cut(s) 328
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 205
HpyAV CCTTC 5 cut(s) 100, 144, 241, 409, 420
HpyCH4III ACNGT 4 cut(s) 85, 148, 397, 431
HpyCH4V TGCA 3 cut(s) 259, 443, 509
HpyF3I CTNAG 2 cut(s) 13, 156
Hsp92II CATG 3 cut(s) 125, 173, 208
Kzo9I GATC 5 cut(s) 9, 16, 185, 235, 348
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 460, 493
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 85, 142, 268
MalI GATC 5 cut(s) 11, 18, 187, 237, 350
MboI GATC 5 cut(s) 9, 16, 185, 235, 348
MboII GAAGA 3 cut(s) 45, 472, 475
MflI RGATCY 4 cut(s) 9, 16, 185, 348
MhlI GDGCHC 1 cut(s) 255
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 8 cut(s) 30, 148, 167, 304, 327, 414, 417, 489
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 2 cut(s) 225, 282
MvaI CCWGG 2 cut(s) 225, 282
NdeII GATC 5 cut(s) 9, 16, 185, 235, 348
NlaIII CATG 3 cut(s) 125, 173, 208
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 85, 268
NspV TTCGAA 1 cut(s) 413
PagI TCATGA 1 cut(s) 204
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 2 cut(s) 475, 508
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 223, 280
PspGI CCWGG 2 cut(s) 223, 280
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 2 cut(s) 227, 418
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 4 cut(s) 9, 16, 185, 348
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 2 cut(s) 474, 507
Sau3AI GATC 5 cut(s) 9, 16, 185, 235, 348
Sau96I GGNCC 2 cut(s) 227, 418
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 2 cut(s) 225, 282
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 111, 129, 162, 229, 246, 315, 359, 388
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 2 cut(s) 227, 418
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 223, 280
TaaI ACNGT 4 cut(s) 85, 148, 397, 431
TaqI TCGA 3 cut(s) 413, 426, 433
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 5 cut(s) 76, 90, 204, 266, 402
TseFI GTSAC 2 cut(s) 85, 268
TseI GCWGC 2 cut(s) 473, 506
Tsp45I GTSAC 2 cut(s) 85, 268
TspDTI ATGAA 2 cut(s) 158, 339
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 5 cut(s) 76, 90, 204, 266, 402
VpaK11BI GGWCC 2 cut(s) 227, 418
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.