MD03G1143000.v1.1

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012758
Physical Location & Seq
Reverse (-)
15309483 .. 15310967
1485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1143000.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGCAATGCTGTCATCAGATCCTCTTGTGGTTGGGAGAGTTATTGGAGATGTTGTTGATTACTTCTCCCCAAGTGTAAAAATGACAGTCTCTTACAACTCCAACAAGAAGGTGTATAATGGGCATGAGCTATTTCCTTCCTCAGTAACCATCAAGCCTAAGGTTGAAGTTCATGGAGGCGATTTGAGGAGTTTCTTTACACTGGTTATGACGGATCCAGATGTTCCCGGCCCCAGTGACCCCTACCTGAAGGAACACTTACACTGGATTGTGACCGACATCCCAGGCACGACTGATAACACATTTGGAATGGAGGTGGTGAAATATGAAATGCCAAGGCCAAACATAGGGATCCACAGGTTTGTGTTTCTTCTGTTCAAGCAGAAAGGCAGGCAGACAGTGATCCCTCCTGCTTCAAAGGACCACTTCAACACCCGAAAATTTGCTGAGGCGAATGACCTTGGCCTTCCTGTGACTGCTGTTTTCTTTAATGCTCAAAGGGAAACTGCTGCAAGGAGACGATAA

Protein Analysis

175

Amino Acids

19.58

Weight (kDa)

9.15

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 52 - 159 9.6e-12 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27550
fragaria_vesca FvH4_3g24700
malus_domestica MD03G1143000.v1.1 MD11G1163500.v1.1
prunus_persica Prupe.6G128400_v2.0.a1 Prupe.6G128400_v2.0.a1
pyrus_communis pycom03g09760
rosa_chinensis RchiOBHm_Chr5g0044251
rosa_laevigata RLG00000034260
rosa_multiflora Rmu_sc0000776.1_g000051
rosa_roxburghii Rroxscaffold_1G00036720
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297200 Rh5BG304600 Rh5CG331500 Rh5DG313500
rosa_wichuraiana Rw0G015760 Rw5G027520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 6 cut(s) 14, 209, 222, 346, 359, 397
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 4 cut(s) 167, 379, 417, 430
AjnI CCWGG 1 cut(s) 283
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
Alw26I GTCTC 2 cut(s) 94, 511
AlwI GGATC 6 cut(s) 14, 209, 222, 346, 359, 397
AoxI GGCC 3 cut(s) 229, 338, 463
ApeKI GCWGC 1 cut(s) 509
ApoI RAATTY 1 cut(s) 440
AspS9I GGNCC 2 cut(s) 230, 421
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 331
AvaII GGWCC 1 cut(s) 421
AxyI CCTNAGG 1 cut(s) 159
BamHI GGATCC 2 cut(s) 214, 351
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 1 cut(s) 496
BccI CCATC 1 cut(s) 158
BciT130I CCWGG 1 cut(s) 285
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 94, 511
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 2 cut(s) 228, 285
Bme18I GGWCC 1 cut(s) 421
BmgT120I GGNCC 2 cut(s) 230, 421
BmiI GGNNCC 3 cut(s) 216, 232, 353
BmrFI CCNGG 2 cut(s) 228, 285
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 3 cut(s) 283, 335, 460
BsaXI ACNNNNNCTCC 2 cut(s) 39, 69
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse1I ACTGG 3 cut(s) 207, 234, 269
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 1 cut(s) 285
BseDI CCNNGG 3 cut(s) 283, 335, 460
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 2 cut(s) 156, 438
BseNI ACTGG 3 cut(s) 207, 234, 269
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 496
BshFI GGCC 3 cut(s) 231, 340, 465
BsiSI CCGG 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 2 cut(s) 94, 511
BsmBI CGTCTC 1 cut(s) 511
BsnI GGCC 3 cut(s) 231, 340, 465
Bsp143I GATC 4 cut(s) 19, 214, 351, 402
BspANI GGCC 3 cut(s) 231, 340, 465
BspCNI CTCAG 2 cut(s) 155, 439
BspLI GGNNCC 3 cut(s) 216, 232, 353
BspPI GGATC 6 cut(s) 14, 209, 222, 346, 359, 397
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 3 cut(s) 207, 234, 269
BssECI CCNNGG 3 cut(s) 283, 335, 460
BssMI GATC 4 cut(s) 19, 214, 351, 402
BssT1I CCWWGG 2 cut(s) 335, 460
Bst2UI CCWGG 1 cut(s) 285
Bst4CI ACNGT 2 cut(s) 88, 400
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 3 cut(s) 142, 159, 447
BstF5I GGATG 1 cut(s) 279
BstKTI GATC 4 cut(s) 22, 217, 354, 405
BstMAI GTCTC 2 cut(s) 94, 511
BstMBI GATC 4 cut(s) 19, 214, 351, 402
BstNI CCWGG 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 226, 283
BstV1I GCAGC 1 cut(s) 496
BstX2I RGATCY 3 cut(s) 19, 214, 351
BstYI RGATCY 3 cut(s) 19, 214, 351
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 231, 340, 465
BtsCI GGATG 1 cut(s) 279
BtsIMutI CAGTG 4 cut(s) 200, 241, 262, 405
Cac8I GCNNGC 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 230, 421
CviAII CATG 2 cut(s) 125, 173
CviJI RGCY 5 cut(s) 130, 157, 231, 340, 465
CviKI_1 RGCY 5 cut(s) 130, 157, 231, 340, 465
DdeI CTNAG 3 cut(s) 142, 159, 447
DpnI GATC 4 cut(s) 21, 216, 353, 404
DpnII GATC 4 cut(s) 19, 214, 351, 402
Eco130I CCWWGG 2 cut(s) 335, 460
Eco47I GGWCC 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 283
EcoT14I CCWWGG 2 cut(s) 335, 460
ErhI CCWWGG 2 cut(s) 335, 460
Esp3I CGTCTC 1 cut(s) 511
FaeI CATG 2 cut(s) 128, 176
FaiI YATR 6 cut(s) 117, 126, 174, 209, 327, 347
FalI AAGNNNNNCTT 4 cut(s) 242, 274, 410, 442
FatI CATG 2 cut(s) 124, 172
Fnu4HI GCNGC 1 cut(s) 510
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 510
GluI GCNGC 1 cut(s) 510
HaeIII GGCC 3 cut(s) 231, 340, 465
HapII CCGG 1 cut(s) 228
Hin1II CATG 2 cut(s) 128, 176
HpaII CCGG 1 cut(s) 228
HphI GGTGA 1 cut(s) 331
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 1 cut(s) 218
HpyAV CCTTC 4 cut(s) 103, 147, 244, 476
HpyCH4III ACNGT 2 cut(s) 88, 400
HpyCH4V TGCA 1 cut(s) 512
HpyF3I CTNAG 3 cut(s) 142, 159, 447
Hsp92II CATG 2 cut(s) 128, 176
Kzo9I GATC 4 cut(s) 19, 214, 351, 402
Lsp1109I GCAGC 1 cut(s) 496
MaeIII GTNAC 4 cut(s) 145, 236, 271, 472
MalI GATC 4 cut(s) 21, 216, 353, 404
MboI GATC 4 cut(s) 19, 214, 351, 402
MboII GAAGA 1 cut(s) 362
MflI RGATCY 3 cut(s) 19, 214, 351
MluCI AATT 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 7 cut(s) 33, 151, 170, 180, 307, 417, 442
MseI TTAA 1 cut(s) 489
MspI CCGG 1 cut(s) 228
MspR9I CCNGG 2 cut(s) 228, 285
MvaI CCWGG 1 cut(s) 285
NciI CCSGG 1 cut(s) 228
NdeII GATC 4 cut(s) 19, 214, 351, 402
NlaIII CATG 2 cut(s) 128, 176
NlaIV GGNNCC 3 cut(s) 216, 232, 353
NmuCI GTSAC 3 cut(s) 236, 271, 472
PkrI GCNGC 1 cut(s) 511
Psp6I CCWGG 1 cut(s) 283
PspGI CCWGG 1 cut(s) 283
PspN4I GGNNCC 3 cut(s) 216, 232, 353
PspPI GGNCC 2 cut(s) 230, 421
PsuI RGATCY 3 cut(s) 19, 214, 351
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 1 cut(s) 510
Sau3AI GATC 4 cut(s) 19, 214, 351, 402
Sau96I GGNCC 2 cut(s) 230, 421
ScrFI CCNGG 2 cut(s) 228, 285
SetI ASST 7 cut(s) 114, 132, 165, 249, 318, 362, 462
SinI GGWCC 1 cut(s) 421
Sse9I AATT 1 cut(s) 440
StyD4I CCNGG 2 cut(s) 226, 283
StyI CCWWGG 2 cut(s) 335, 460
TaaI ACNGT 2 cut(s) 88, 400
TaqII GACCGA 1 cut(s) 290
TasI AATT 1 cut(s) 440
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TscAI CASTG 4 cut(s) 207, 241, 269, 405
TseFI GTSAC 3 cut(s) 236, 271, 472
TseI GCWGC 1 cut(s) 509
Tsp45I GTSAC 3 cut(s) 236, 271, 472
TspDTI ATGAA 2 cut(s) 161, 342
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 4 cut(s) 207, 241, 269, 405
VpaK11BI GGWCC 1 cut(s) 421
XapI RAATTY 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.