Rw0G015760

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00734
OG0012758
Physical Location & Seq
Forward (+)
43307 .. 45167
1861 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015760.1

Sequence Viewer

Length: 522 bp
ATGACGAAAATCTTATATCCTCTTGTTGTTGGAAGAGTCATTGGTGATGTTGTTGATTATTTCTCCCCCAGTGTTAAAATGGCAGTGACTTACAACTCCAGCAAGAAGGTGTATAATGGGCATGAGCTATTTCCTTCCTCAGTAACCACGAAGCCTAAGGTTGAAGTTCAGGGAGGCGATCTGAGATCTTTCTTTACACTGGTCATGACTGACCCAGATGTTCCAGGTCCTAGTGATCCATATCTGAAGGAGCACTTGCACTGGATAGTCACAGATATCCCAGGAACAACTGACAACACATTTGGAAGGGAGGTGGTGAATAATGAAATGCCGAGGCCAAACATAGGGATCCACAGGTTTGTGTTCCTTCTGTTCAAGCAGAAAGGTAGGCAAACAGTGATTCCTCCTCCTTCCAAGGACAACTTCGACAGTCGAAAGTTTGCAGAAGCAAACGAGTTCGGGCTTCCGGTGGCTGCTGTTTTCTTCAATGCCCAGAGGGAAACTGCTGCAAGAAGACGCTAA

Protein Analysis

173

Amino Acids

19.5

Weight (kDa)

9.36

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 50 - 150 3.9e-10 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27550
fragaria_vesca FvH4_3g24700
malus_domestica MD03G1143000.v1.1 MD11G1163500.v1.1
prunus_persica Prupe.6G128400_v2.0.a1 Prupe.6G128400_v2.0.a1
pyrus_communis pycom03g09760
rosa_chinensis RchiOBHm_Chr5g0044251
rosa_laevigata RLG00000034260
rosa_multiflora Rmu_sc0000776.1_g000051
rosa_roxburghii Rroxscaffold_1G00036720
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297200 Rh5BG304600 Rh5CG331500 Rh5DG313500
rosa_wichuraiana Rw0G015760 Rw5G027520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 230, 343, 356
AcuI CTGAAG 1 cut(s) 266
AfiI CCNNNNNNNGG 1 cut(s) 344
AgsI TTSAA 3 cut(s) 164, 376, 487
AjnI CCWGG 2 cut(s) 223, 280
AjuI GAANNNNNNNTTGG 2 cut(s) 407, 439
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 3 cut(s) 230, 343, 356
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 2 cut(s) 473, 506
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 56, 328
AvaII GGWCC 1 cut(s) 227
AxyI CCTNAGG 1 cut(s) 156
BamHI GGATCC 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 2 cut(s) 460, 493
BciT130I CCWGG 2 cut(s) 225, 282
BfaI CTAG 1 cut(s) 231
BglII AGATCT 1 cut(s) 185
BisI GCNGC 2 cut(s) 474, 507
BlsI GCNGC 2 cut(s) 475, 508
Bme1390I CCNGG 2 cut(s) 225, 282
Bme18I GGWCC 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 350
BmrFI CCNGG 2 cut(s) 225, 282
BmrI ACTGGG 1 cut(s) 63
BmuI ACTGGG 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 82
BsaBI GATNNNNATC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 280, 332, 414
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 344
Bse1I ACTGG 3 cut(s) 69, 204, 266
Bse21I CCTNAGG 1 cut(s) 156
Bse8I GATNNNNATC 1 cut(s) 240
BseBI CCWGG 2 cut(s) 225, 282
BseDI CCNNGG 3 cut(s) 280, 332, 414
BseJI GATNNNNATC 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 2 cut(s) 153, 173
BseNI ACTGG 3 cut(s) 69, 204, 266
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 2 cut(s) 460, 493
BshFI GGCC 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 344
BsnI GGCC 1 cut(s) 337
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 4 cut(s) 178, 185, 235, 348
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 152, 174
BspHI TCATGA 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 350
BspPI GGATC 3 cut(s) 230, 343, 356
BsrI ACTGG 3 cut(s) 69, 204, 266
BssECI CCNNGG 3 cut(s) 280, 332, 414
BssMI GATC 4 cut(s) 178, 185, 235, 348
BssT1I CCWWGG 1 cut(s) 414
Bst2UI CCWGG 2 cut(s) 225, 282
Bst4CI ACNGT 2 cut(s) 397, 431
Bst6I CTCTTC 1 cut(s) 28
BstDEI CTNAG 3 cut(s) 139, 156, 182
BstKTI GATC 4 cut(s) 181, 188, 238, 351
BstMBI GATC 4 cut(s) 178, 185, 235, 348
BstNI CCWGG 2 cut(s) 225, 282
BstSCI CCNGG 2 cut(s) 223, 280
BstV1I GCAGC 2 cut(s) 460, 493
BstX2I RGATCY 2 cut(s) 185, 348
BstYI RGATCY 2 cut(s) 185, 348
Bsu36I CCTNAGG 1 cut(s) 156
BsuRI GGCC 1 cut(s) 337
BtsI GCAGTG 1 cut(s) 90
BtsIMutI CAGTG 5 cut(s) 76, 90, 197, 259, 402
CciI TCATGA 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 227
CviAII CATG 2 cut(s) 122, 205
CviJI RGCY 5 cut(s) 127, 154, 337, 463, 473
CviKI_1 RGCY 5 cut(s) 127, 154, 337, 463, 473
DdeI CTNAG 3 cut(s) 139, 156, 182
DpnI GATC 4 cut(s) 180, 187, 237, 350
DpnII GATC 4 cut(s) 178, 185, 235, 348
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 414
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 266
Eco81I CCTNAGG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 227
EcoRII CCWGG 2 cut(s) 223, 280
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 2 cut(s) 125, 208
FaiI YATR 6 cut(s) 16, 114, 123, 206, 241, 344
FalI AAGNNNNNCTT 4 cut(s) 239, 271, 407, 439
FatI CATG 2 cut(s) 121, 204
Fnu4HI GCNGC 2 cut(s) 474, 507
Fsp4HI GCNGC 2 cut(s) 474, 507
FspBI CTAG 1 cut(s) 231
GluI GCNGC 2 cut(s) 474, 507
GsuI CTGGAG 1 cut(s) 82
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 467
Hin1II CATG 2 cut(s) 125, 208
HinfI GANTC 2 cut(s) 36, 400
HpaII CCGG 1 cut(s) 467
HphI GGTGA 2 cut(s) 56, 328
Hpy188I TCNGA 2 cut(s) 183, 246
Hpy188III TCNNGA 1 cut(s) 205
HpyAV CCTTC 6 cut(s) 100, 144, 241, 300, 377, 420
HpyCH4III ACNGT 2 cut(s) 397, 431
HpyCH4V TGCA 3 cut(s) 259, 443, 509
HpyF3I CTNAG 3 cut(s) 139, 156, 182
Hsp92II CATG 2 cut(s) 125, 208
Kzo9I GATC 4 cut(s) 178, 185, 235, 348
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 460, 493
MaeI CTAG 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 85, 142, 268
MalI GATC 4 cut(s) 180, 187, 237, 350
MboI GATC 4 cut(s) 178, 185, 235, 348
MboII GAAGA 2 cut(s) 45, 475
MflI RGATCY 2 cut(s) 185, 348
MhlI GDGCHC 1 cut(s) 255
MlyI GAGTC 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 10
MnlI CCTC 8 cut(s) 30, 148, 167, 304, 327, 414, 417, 489
MseI TTAA 1 cut(s) 75
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 2 cut(s) 225, 282
MvaI CCWGG 2 cut(s) 225, 282
NdeII GATC 4 cut(s) 178, 185, 235, 348
NlaIII CATG 2 cut(s) 125, 208
NlaIV GGNNCC 1 cut(s) 350
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 85, 268
PagI TCATGA 1 cut(s) 204
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 2 cut(s) 475, 508
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 227
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 223, 280
PspGI CCWGG 2 cut(s) 223, 280
PspN4I GGNNCC 1 cut(s) 350
PspPI GGNCC 1 cut(s) 227
PspPPI RGGWCCY 1 cut(s) 227
PsuI RGATCY 2 cut(s) 185, 348
SaqAI TTAA 1 cut(s) 75
SatI GCNGC 2 cut(s) 474, 507
Sau3AI GATC 4 cut(s) 178, 185, 235, 348
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 45
ScrFI CCNGG 2 cut(s) 225, 282
SduI GDGCHC 1 cut(s) 255
SetI ASST 7 cut(s) 111, 129, 162, 229, 315, 359, 388
SinI GGWCC 1 cut(s) 227
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 223, 280
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 2 cut(s) 397, 431
TaqI TCGA 2 cut(s) 426, 433
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 75
Tru9I TTAA 1 cut(s) 75
TscAI CASTG 5 cut(s) 76, 90, 204, 266, 402
TseFI GTSAC 2 cut(s) 85, 268
TseI GCWGC 2 cut(s) 473, 506
Tsp45I GTSAC 2 cut(s) 85, 268
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 5 cut(s) 76, 90, 204, 266, 402
VpaK11BI GGWCC 1 cut(s) 227
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.