RLG00000034260

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012758
Physical Location & Seq
Reverse (-)
39533694 .. 39534526
833 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034260

Sequence Viewer

Length: 513 bp
ATGTCAGATCCTCTTGTTGTTGGAAGAGTCATTGGAGATGTTGTTGATTATTTCTCCCCCAGTGTTAAAATGACAGTGACTTACAACTCCAGCAAGAAGGTGTATAATGGGCATGAGCTATTTCCTTCGTCAGTAACCACGAAGCCTAAGGTTGAAGTTCAGGGAGGCGATCTGAGATCTTTCTTTACACTGGTCATGACTGACCCAGATGTTCCAGGTCCTAGTGATCCATATCTGAAGGAGCACTTGCACTGGATAGTCACAGATATCCCAGGAACAACTGACAACACATTTGGAAGGGAGGTGGTGAAATATGAAATGCCGAGGCCAAACATAGGGATCCACAGGTTTGTGTTCCTTCTGTTCAAGCAGAAAGGTAGGCAAACAGTGATTCCTCCTCCTTCCAAGGACCACTTCGACAGTCGAAAGTTTGCAGAAGCAAACGAGTTCGGGCTTCCGGTGGCTGCTGTTTTCTTCAATGCCCAGAGGGAAACTGCTGCAAGAAGACGCTAA

Protein Analysis

171

Amino Acids

19.2

Weight (kDa)

9.15

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 46 - 145 8.9e-12 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012758)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27550
fragaria_vesca FvH4_3g24700
malus_domestica MD03G1143000.v1.1 MD11G1163500.v1.1
prunus_persica Prupe.6G128400_v2.0.a1 Prupe.6G128400_v2.0.a1
pyrus_communis pycom03g09760
rosa_chinensis RchiOBHm_Chr5g0044251
rosa_laevigata RLG00000034260
rosa_multiflora Rmu_sc0000776.1_g000051
rosa_roxburghii Rroxscaffold_1G00036720
rosa_rugosa Rorug05G0212900
rosa_samantha Rh5AG297200 Rh5BG304600 Rh5CG331500 Rh5DG313500
rosa_wichuraiana Rw0G015760 Rw5G027520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 2, 221, 334, 347
AcuI CTGAAG 1 cut(s) 257
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 3 cut(s) 155, 367, 478
AjnI CCWGG 2 cut(s) 214, 271
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
AluBI AGCT 1 cut(s) 118
AluI AGCT 1 cut(s) 118
Alw21I GWGCWC 1 cut(s) 246
AlwI GGATC 4 cut(s) 2, 221, 334, 347
AoxI GGCC 1 cut(s) 326
ApeKI GCWGC 2 cut(s) 464, 497
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspS9I GGNCC 2 cut(s) 218, 409
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 2 cut(s) 218, 409
AxyI CCTNAGG 1 cut(s) 147
BamHI GGATCC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 451, 484
BciT130I CCWGG 2 cut(s) 216, 273
BfaI CTAG 1 cut(s) 222
BglII AGATCT 1 cut(s) 176
BisI GCNGC 2 cut(s) 465, 498
BlsI GCNGC 2 cut(s) 466, 499
Bme1390I CCNGG 2 cut(s) 216, 273
Bme18I GGWCC 2 cut(s) 218, 409
BmgT120I GGNCC 2 cut(s) 218, 409
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 2 cut(s) 216, 273
BmrI ACTGGG 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 54
BpmI CTGGAG 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 271, 323, 405
BsaWI WCCGGW 1 cut(s) 457
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 3 cut(s) 60, 195, 257
Bse21I CCTNAGG 1 cut(s) 147
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 2 cut(s) 216, 273
BseDI CCNNGG 3 cut(s) 271, 323, 405
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 1 cut(s) 164
BseNI ACTGG 3 cut(s) 60, 195, 257
BseRI GAGGAG 1 cut(s) 387
BseXI GCAGC 2 cut(s) 451, 484
BshFI GGCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 458
BslI CCNNNNNNNGG 1 cut(s) 335
BsnI GGCC 1 cut(s) 328
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 5 cut(s) 7, 169, 176, 226, 339
BspANI GGCC 1 cut(s) 328
BspCNI CTCAG 1 cut(s) 165
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 341
BspPI GGATC 4 cut(s) 2, 221, 334, 347
BsrI ACTGG 3 cut(s) 60, 195, 257
BssECI CCNNGG 3 cut(s) 271, 323, 405
BssMI GATC 5 cut(s) 7, 169, 176, 226, 339
BssT1I CCWWGG 1 cut(s) 405
Bst2UI CCWGG 2 cut(s) 216, 273
Bst4CI ACNGT 3 cut(s) 76, 388, 422
Bst6I CTCTTC 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 147, 173
BstKTI GATC 5 cut(s) 10, 172, 179, 229, 342
BstMBI GATC 5 cut(s) 7, 169, 176, 226, 339
BstNI CCWGG 2 cut(s) 216, 273
BstSCI CCNGG 2 cut(s) 214, 271
BstV1I GCAGC 2 cut(s) 451, 484
BstX2I RGATCY 3 cut(s) 7, 176, 339
BstYI RGATCY 3 cut(s) 7, 176, 339
Bsu36I CCTNAGG 1 cut(s) 147
BsuRI GGCC 1 cut(s) 328
BtsIMutI CAGTG 5 cut(s) 67, 81, 188, 250, 393
CciI TCATGA 1 cut(s) 195
Cfr13I GGNCC 2 cut(s) 218, 409
CviAII CATG 2 cut(s) 113, 196
CviJI RGCY 5 cut(s) 118, 145, 328, 454, 464
CviKI_1 RGCY 5 cut(s) 118, 145, 328, 454, 464
DdeI CTNAG 2 cut(s) 147, 173
DpnI GATC 5 cut(s) 9, 171, 178, 228, 341
DpnII GATC 5 cut(s) 7, 169, 176, 226, 339
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Eco130I CCWWGG 1 cut(s) 405
Eco32I GATATC 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 218, 409
Eco57I CTGAAG 1 cut(s) 257
Eco81I CCTNAGG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 214, 271
EcoRV GATATC 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 405
FaeI CATG 2 cut(s) 116, 199
FaiI YATR 6 cut(s) 105, 114, 197, 232, 315, 335
FalI AAGNNNNNCTT 4 cut(s) 230, 262, 398, 430
FatI CATG 2 cut(s) 112, 195
Fnu4HI GCNGC 2 cut(s) 465, 498
Fsp4HI GCNGC 2 cut(s) 465, 498
FspBI CTAG 1 cut(s) 222
GluI GCNGC 2 cut(s) 465, 498
GsuI CTGGAG 1 cut(s) 73
HaeIII GGCC 1 cut(s) 328
HapII CCGG 1 cut(s) 458
Hin1II CATG 2 cut(s) 116, 199
HinfI GANTC 2 cut(s) 27, 391
HpaII CCGG 1 cut(s) 458
HphI GGTGA 1 cut(s) 319
Hpy188I TCNGA 3 cut(s) 7, 174, 237
Hpy188III TCNNGA 1 cut(s) 196
HpyAV CCTTC 6 cut(s) 91, 135, 232, 291, 368, 411
HpyCH4III ACNGT 3 cut(s) 76, 388, 422
HpyCH4V TGCA 3 cut(s) 250, 434, 500
HpyF3I CTNAG 2 cut(s) 147, 173
Hsp92II CATG 2 cut(s) 116, 199
Kzo9I GATC 5 cut(s) 7, 169, 176, 226, 339
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 2 cut(s) 451, 484
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 76, 133, 259
MalI GATC 5 cut(s) 9, 171, 178, 228, 341
MboI GATC 5 cut(s) 7, 169, 176, 226, 339
MboII GAAGA 2 cut(s) 36, 466
MflI RGATCY 3 cut(s) 7, 176, 339
MhlI GDGCHC 1 cut(s) 246
MlyI GAGTC 1 cut(s) 36
MnlI CCTC 7 cut(s) 21, 158, 295, 318, 405, 408, 480
MseI TTAA 1 cut(s) 66
MspI CCGG 1 cut(s) 458
MspR9I CCNGG 2 cut(s) 216, 273
MvaI CCWGG 2 cut(s) 216, 273
NdeII GATC 5 cut(s) 7, 169, 176, 226, 339
NlaIII CATG 2 cut(s) 116, 199
NlaIV GGNNCC 1 cut(s) 341
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 2 cut(s) 76, 259
PagI TCATGA 1 cut(s) 195
PfeI GAWTC 1 cut(s) 391
PkrI GCNGC 2 cut(s) 466, 499
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
Psp6I CCWGG 2 cut(s) 214, 271
PspGI CCWGG 2 cut(s) 214, 271
PspN4I GGNNCC 1 cut(s) 341
PspPI GGNCC 2 cut(s) 218, 409
PspPPI RGGWCCY 1 cut(s) 218
PsuI RGATCY 3 cut(s) 7, 176, 339
SaqAI TTAA 1 cut(s) 66
SatI GCNGC 2 cut(s) 465, 498
Sau3AI GATC 5 cut(s) 7, 169, 176, 226, 339
Sau96I GGNCC 2 cut(s) 218, 409
SchI GAGTC 1 cut(s) 36
ScrFI CCNGG 2 cut(s) 216, 273
SduI GDGCHC 1 cut(s) 246
SetI ASST 7 cut(s) 102, 120, 153, 220, 306, 350, 379
SinI GGWCC 2 cut(s) 218, 409
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 2 cut(s) 214, 271
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 3 cut(s) 76, 388, 422
TaqI TCGA 2 cut(s) 417, 424
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 66
Tru9I TTAA 1 cut(s) 66
TscAI CASTG 5 cut(s) 67, 81, 195, 257, 393
TseFI GTSAC 2 cut(s) 76, 259
TseI GCWGC 2 cut(s) 464, 497
Tsp45I GTSAC 2 cut(s) 76, 259
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 5 cut(s) 67, 81, 195, 257, 393
VpaK11BI GGWCC 2 cut(s) 218, 409
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.