FvH4_3g25460

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014204
Physical Location & Seq
Forward (+)
18366256 .. 18370695
4440 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25460.t1

Sequence Viewer

Length: 1176 bp
ATGGAAGACCTGAAAGCGAAGACCCCAGCATCATGCAAAGACCTCCCAGCTCTCCTAACCTCTTTCGTCGACACCTTCGTCGACTTCTCCGTCACCGGACACTTCCTCTTACCCGAAACCCCCACTCCCCCTCAACCCTTGCAAACCTACTACCCTCCGCAGGACCGTCTCATCGCCATCGGCGACCTCCACGGCGACCTCGCCAAGACCAAGCAATCCCTCCGCCTCGCCAATCTCATTGACGCCTCCGACAAATGGGCCGGAGGATCCGCCACCGTGGTCCAGGTCGGCGACGTGCTCGACCGTGGCGGCGACGAGCTCAAGATCCTCTATTTCCTCGAGAAGCTGCGACGCGAAGCCGCCAGATGCGGCGGCACCGTGATTACCATGCACGGCAACCACGAGATCATGAATGTGGAAGGAGATTTCAGGTACGCCACCAAGGAGGGTTTGGATGAGTTTGTGAATTGGGCACATTGGTATACAACTGGTAACAGGATGAAGTCTCTTTGTAAAGGCTTGGAGAAGCCGCGGGACCCGTTCGAGGGGCTGCCAAGTGTATTCAATAATGTGAAGGGCGAGTCTGCAGAGGGTTTTCGGGCCAGAATTGCCGCGCTGAGGCCTGATGGTCCAGTTACTAGTCGGTTCTTGTCCAAGAATGTCACTGTGTTGGTTATAGGGGACTCGGTTTTCGTCCACGGCGGGCTTTTGGCAAAGCATGTTAAGTATGGACTAGAGAAGATGAATGAGGAGGTGAGGGAGTGGATTAATGGGGTGAAGGGGAGACTGGCGCCGGAGTATTGCCGCGGGGGGAACTCTGTGGTTTGGTTGAGGAAGTTTTCTGATGAAATCGCCGACAAGTGTGATTGTTCGGCGCTGGAACATGTTTTGGCAACTATTCCTGGTGCTAAGAGGATGATCATGGGTCATACCATTCAAGAGTTTGGGATCAATGGTGCTTGCAATGAGAGAGCTATTCGGATTGATGTGGGGATGTCCAAGGGCTGTGGTAATGGCTTGCCTGAAGTTTTGGAGATAAATGGGAATTCGAGTTTGAGGATCCTGACGTCGAATCCGTTGTACCAGAAGAAGTATGCATCTTCTGCTGAAGCTGGGAGGAGGGAAGGGGTTGGATTGCTGGAGCATGGACCTCAACAAGTGGAAGTGAAAGCTTGA

Protein Analysis

392

Amino Acids

42.91

Weight (kDa)

6.8

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 56 - 169 4.2e-09 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18480
fragaria_vesca FvH4_3g25460 FvH4_3g25460 FvH4_3g25460
malus_domestica MD02G1292100.v1.1 MD07G1034500.v1.1
prunus_persica Prupe.2G031200_v2.0.a1
pyrus_communis pycom02g24670
rosa_chinensis RchiOBHm_Chr4g0435781
rosa_laevigata RLG00000006559
rosa_multiflora Rmu_sc0000051.1_g000023 Rmu_sc0004389.1_g000004
rosa_roxburghii Rroxscaffold_5G00376970
rosa_rugosa Rorug04G0289600
rosa_samantha Rh4BG352600 Rh4CG367100
rosa_wichuraiana Rw4G030030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 77, 89
AatII GACGTC 1 cut(s) 1070
AccB1I GGYRCC 2 cut(s) 374, 790
AccI GTMKAC 3 cut(s) 69, 81, 482
AccII CGCG 4 cut(s) 354, 532, 614, 807
AclWI GGATC 6 cut(s) 261, 274, 319, 956, 1054, 1067
AcsI RAATTY 1 cut(s) 1045
AcuI CTGAAG 2 cut(s) 1044, 1128
AcyI GRCGYC 3 cut(s) 243, 791, 1067
AfaI GTAC 2 cut(s) 434, 1082
AfiI CCNNNNNNNGG 5 cut(s) 160, 255, 544, 545, 618
AflIII ACRYGT 1 cut(s) 883
AgsI TTSAA 2 cut(s) 565, 938
AhlI ACTAGT 1 cut(s) 638
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 2 cut(s) 282, 901
AluBI AGCT 6 cut(s) 50, 319, 346, 974, 1112, 1172
AluI AGCT 6 cut(s) 50, 319, 346, 974, 1112, 1172
Alw21I GWGCWC 2 cut(s) 300, 321
Alw26I GTCTC 3 cut(s) 173, 510, 778
AlwI GGATC 6 cut(s) 261, 274, 319, 956, 1054, 1067
Ama87I CYCGRG 1 cut(s) 338
AoxI GGCC 3 cut(s) 258, 600, 620
ApeKI GCWGC 2 cut(s) 346, 550
ApoI RAATTY 1 cut(s) 1045
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 3 cut(s) 616, 793, 877
AspS9I GGNCC 7 cut(s) 163, 258, 280, 535, 600, 629, 1148
AsuHPI GGTGA 3 cut(s) 85, 766, 787
AvaI CYCGRG 1 cut(s) 338
AvaII GGWCC 5 cut(s) 163, 280, 535, 629, 1148
BaeGI GKGCMC 1 cut(s) 475
BamHI GGATCC 2 cut(s) 266, 1059
BanI GGYRCC 2 cut(s) 374, 790
BanII GRGCYC 1 cut(s) 321
BauI CACGAG 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 12, 26
Bbv12I GWGCWC 2 cut(s) 300, 321
BbvCI CCTCAGC 1 cut(s) 617
BbvI GCAGC 2 cut(s) 333, 537
BccI CCATC 2 cut(s) 185, 620
BceAI ACGGC 3 cut(s) 208, 409, 715
BciT130I CCWGG 2 cut(s) 284, 903
BclI TGATCA 1 cut(s) 918
BcoDI GTCTC 3 cut(s) 173, 510, 778
BcuI ACTAGT 1 cut(s) 638
BfaI CTAG 2 cut(s) 639, 734
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 2 cut(s) 794, 878
BisI GCNGC 9 cut(s) 310, 347, 360, 370, 373, 530, 551, 612, 805
BlsI GCNGC 9 cut(s) 311, 348, 361, 371, 374, 531, 552, 613, 806
Bme1390I CCNGG 2 cut(s) 284, 903
Bme18I GGWCC 5 cut(s) 163, 280, 535, 629, 1148
BmeT110I CYCGRG 1 cut(s) 338
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 7 cut(s) 163, 258, 280, 535, 600, 629, 1148
BmiI GGNNCC 6 cut(s) 268, 376, 536, 537, 792, 1061
BmrFI CCNGG 2 cut(s) 284, 903
BmsI GCATC 3 cut(s) 38, 356, 1106
BpiI GAAGAC 2 cut(s) 12, 26
BpmI CTGGAG 1 cut(s) 1160
Bpu10I CCTNAGC 1 cut(s) 617
BpuEI CTTGAG 1 cut(s) 305
BsaHI GRCGYC 3 cut(s) 243, 791, 1067
BsaJI CCNNGG 8 cut(s) 190, 276, 304, 441, 530, 697, 805, 999
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
Bsc4I CCNNNNNNNGG 5 cut(s) 160, 255, 544, 545, 618
Bse1I ACTGG 3 cut(s) 493, 632, 792
Bse3DI GCAATG 1 cut(s) 970
BseBI CCWGG 2 cut(s) 284, 903
BseDI CCNNGG 8 cut(s) 190, 276, 304, 441, 530, 697, 805, 999
BseGI GGATG 4 cut(s) 460, 504, 921, 999
BseLI CCNNNNNNNGG 5 cut(s) 160, 255, 544, 545, 618
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 1 cut(s) 608
BseNI ACTGG 3 cut(s) 493, 632, 792
BseRI GAGGAG 2 cut(s) 764, 1132
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 2 cut(s) 333, 537
BseYI CCCAGC 3 cut(s) 25, 46, 1112
Bsh1236I CGCG 4 cut(s) 354, 532, 614, 807
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 3 cut(s) 260, 602, 622
BshNI GGYRCC 2 cut(s) 374, 790
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 2 cut(s) 300, 321
BsiHKCI CYCGRG 1 cut(s) 338
BsiSI CCGG 3 cut(s) 96, 261, 794
BslFI GGGAC 2 cut(s) 548, 695
BslI CCNNNNNNNGG 5 cut(s) 160, 255, 544, 545, 618
BsmAI GTCTC 3 cut(s) 173, 510, 778
BsmBI CGTCTC 1 cut(s) 173
BsmFI GGGAC 2 cut(s) 548, 695
BsnI GGCC 3 cut(s) 260, 602, 622
BsoBI CYCGRG 1 cut(s) 338
Bsp1286I GDGCHC 3 cut(s) 300, 321, 475
Bsp143I GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
BspANI GGCC 3 cut(s) 260, 602, 622
BspCNI CTCAG 1 cut(s) 609
BspFNI CGCG 4 cut(s) 354, 532, 614, 807
BspHI TCATGA 1 cut(s) 408
BspLI GGNNCC 6 cut(s) 268, 376, 536, 537, 792, 1061
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 6 cut(s) 261, 274, 319, 956, 1054, 1067
BspT107I GGYRCC 2 cut(s) 374, 790
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 3 cut(s) 493, 632, 792
BssECI CCNNGG 8 cut(s) 190, 276, 304, 441, 530, 697, 805, 999
BssMI GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
BssNAI GTATAC 1 cut(s) 483
BssNI GRCGYC 3 cut(s) 243, 791, 1067
BssSI CACGAG 1 cut(s) 401
BssT1I CCWWGG 2 cut(s) 441, 999
Bst1107I GTATAC 1 cut(s) 483
Bst2BI CACGAG 1 cut(s) 401
Bst2UI CCWGG 2 cut(s) 284, 903
Bst4CI ACNGT 5 cut(s) 167, 277, 305, 379, 667
BstACI GRCGYC 3 cut(s) 243, 791, 1067
BstAPI GCANNNNNTGC 1 cut(s) 1103
BstC8I GCNNGC 3 cut(s) 704, 961, 1019
BstDEI CTNAG 2 cut(s) 617, 909
BstDSI CCRYGG 6 cut(s) 190, 276, 304, 530, 697, 805
BstF5I GGATG 4 cut(s) 460, 504, 921, 999
BstFNI CGCG 4 cut(s) 354, 532, 614, 807
BstH2I RGCGCY 2 cut(s) 794, 878
BstHHI GCGC 3 cut(s) 616, 793, 877
BstKTI GATC 6 cut(s) 269, 327, 408, 921, 951, 1062
BstMAI GTCTC 3 cut(s) 173, 510, 778
BstMBI GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 2 cut(s) 608, 1103
BstNI CCWGG 2 cut(s) 284, 903
BstNSI RCATGY 2 cut(s) 722, 887
BstSCI CCNGG 2 cut(s) 282, 901
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 4 cut(s) 354, 532, 614, 807
BstV1I GCAGC 2 cut(s) 333, 537
BstV2I GAAGAC 2 cut(s) 12, 26
BstX2I RGATCY 3 cut(s) 266, 324, 1059
BstYI RGATCY 3 cut(s) 266, 324, 1059
BstZ17I GTATAC 1 cut(s) 483
BsuRI GGCC 3 cut(s) 260, 602, 622
BtgI CCRYGG 6 cut(s) 190, 276, 304, 530, 697, 805
BtgZI GCGATG 1 cut(s) 157
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 4 cut(s) 460, 504, 921, 999
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 704, 961, 1019
CciI TCATGA 1 cut(s) 408
CfoI GCGC 3 cut(s) 616, 793, 877
Cfr13I GGNCC 7 cut(s) 163, 258, 280, 535, 600, 629, 1148
Cfr42I CCGCGG 2 cut(s) 533, 808
CseI GACGC 2 cut(s) 251, 360
Csp6I GTAC 2 cut(s) 433, 1081
CspCI CAANNNNNGTGG 2 cut(s) 988, 1023
CviAII CATG 7 cut(s) 33, 388, 409, 719, 884, 922, 1145
CviQI GTAC 2 cut(s) 433, 1081
DdeI CTNAG 2 cut(s) 617, 909
DinI GGCGCC 1 cut(s) 792
DpnI GATC 6 cut(s) 268, 326, 407, 920, 950, 1061
DpnII GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
DrdI GACNNNNNNGTC 2 cut(s) 77, 89
DseDI GACNNNNNNGTC 2 cut(s) 77, 89
EciI GGCGGA 2 cut(s) 212, 259
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 441, 999
Eco147I AGGCCT 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 5 cut(s) 163, 280, 535, 629, 1148
Eco53kI GAGCTC 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 1044, 1128
Eco88I CYCGRG 1 cut(s) 338
EcoICRI GAGCTC 1 cut(s) 319
EcoO109I RGGNCCY 1 cut(s) 535
EcoRI GAATTC 1 cut(s) 1045
EcoRII CCWGG 2 cut(s) 282, 901
EcoT14I CCWWGG 2 cut(s) 441, 999
EcoT22I ATGCAT 1 cut(s) 1099
EcoT38I GRGCYC 1 cut(s) 321
EgeI GGCGCC 1 cut(s) 792
EheI GGCGCC 1 cut(s) 792
ErhI CCWWGG 2 cut(s) 441, 999
Esp3I CGTCTC 1 cut(s) 173
FaeI CATG 7 cut(s) 36, 391, 412, 722, 887, 925, 1148
FaqI GGGAC 2 cut(s) 548, 695
FatI CATG 7 cut(s) 32, 387, 408, 718, 883, 921, 1144
FauI CCCGC 3 cut(s) 525, 695, 800
FbaI TGATCA 1 cut(s) 918
FblI GTMKAC 3 cut(s) 69, 81, 482
Fnu4HI GCNGC 9 cut(s) 310, 347, 360, 370, 373, 530, 551, 612, 805
FokI GGATG 4 cut(s) 467, 511, 928, 1006
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 9 cut(s) 310, 347, 360, 370, 373, 530, 551, 612, 805
FspBI CTAG 2 cut(s) 639, 734
GlaI GCGC 3 cut(s) 615, 792, 876
GluI GCNGC 9 cut(s) 310, 347, 360, 370, 373, 530, 551, 612, 805
GsaI CCCAGC 3 cut(s) 29, 50, 1116
GsuI CTGGAG 1 cut(s) 1160
HaeII RGCGCY 2 cut(s) 794, 878
HaeIII GGCC 3 cut(s) 260, 602, 622
HapII CCGG 3 cut(s) 96, 261, 794
HgaI GACGC 2 cut(s) 251, 360
HhaI GCGC 3 cut(s) 616, 793, 877
Hin1I GRCGYC 3 cut(s) 243, 791, 1067
Hin1II CATG 7 cut(s) 36, 391, 412, 722, 887, 925, 1148
Hin6I GCGC 3 cut(s) 614, 791, 875
HinP1I GCGC 3 cut(s) 614, 791, 875
HincII GTYRAC 2 cut(s) 70, 82
HindII GTYRAC 2 cut(s) 70, 82
HindIII AAGCTT 1 cut(s) 1170
HinfI GANTC 3 cut(s) 581, 683, 1072
HpaII CCGG 3 cut(s) 96, 261, 794
HphI GGTGA 3 cut(s) 85, 766, 787
Hpy166II GTNNAC 4 cut(s) 70, 82, 483, 697
Hpy188I TCNGA 3 cut(s) 250, 844, 981
Hpy188III TCNNGA 5 cut(s) 322, 340, 409, 938, 1063
Hpy8I GTNNAC 4 cut(s) 70, 82, 483, 697
Hpy99I CGWCG 6 cut(s) 71, 83, 296, 317, 354, 1072
HpyAV CCTTC 5 cut(s) 85, 413, 568, 772, 1118
HpyCH4III ACNGT 5 cut(s) 167, 277, 305, 379, 667
HpyCH4IV ACGT 2 cut(s) 294, 1067
HpyCH4V TGCA 6 cut(s) 36, 142, 391, 587, 963, 1097
HpyF10VI GCNNNNNNNGC 2 cut(s) 608, 1103
HpyF3I CTNAG 2 cut(s) 617, 909
HpySE526I ACGT 2 cut(s) 294, 1067
Hsp92I GRCGYC 3 cut(s) 243, 791, 1067
Hsp92II CATG 7 cut(s) 36, 391, 412, 722, 887, 925, 1148
HspAI GCGC 3 cut(s) 614, 791, 875
KasI GGCGCC 1 cut(s) 790
KflI GGGWCCC 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 918
KspI CCGCGG 2 cut(s) 533, 808
Kzo9I GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
LmnI GCTCC 1 cut(s) 1141
Lsp1109I GCAGC 2 cut(s) 333, 537
LweI GCATC 3 cut(s) 38, 356, 1106
MaeI CTAG 2 cut(s) 639, 734
MaeII ACGT 2 cut(s) 294, 1067
MaeIII GTNAC 4 cut(s) 91, 491, 634, 661
MalI GATC 6 cut(s) 268, 326, 407, 920, 950, 1061
MboI GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
MboII GAAGA 5 cut(s) 17, 31, 751, 1092, 1099
MflI RGATCY 3 cut(s) 266, 324, 1059
MhlI GDGCHC 3 cut(s) 300, 321, 475
MluCI AATT 3 cut(s) 466, 606, 1045
Mly113I GGCGCC 1 cut(s) 791
MlyI GAGTC 2 cut(s) 590, 677
MmeI TCCRAC 2 cut(s) 273, 1111
Mph1103I ATGCAT 1 cut(s) 1099
MseI TTAA 2 cut(s) 723, 768
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 2 cut(s) 532, 807
MspI CCGG 3 cut(s) 96, 261, 794
MspR9I CCNGG 2 cut(s) 284, 903
MvaI CCWGG 2 cut(s) 284, 903
MvnI CGCG 4 cut(s) 354, 532, 614, 807
MwoI GCNNNNNNNGC 2 cut(s) 608, 1103
NarI GGCGCC 1 cut(s) 791
NdeII GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
NlaIII CATG 7 cut(s) 36, 391, 412, 722, 887, 925, 1148
NlaIV GGNNCC 6 cut(s) 268, 376, 536, 537, 792, 1061
NmuCI GTSAC 2 cut(s) 91, 661
NsiI ATGCAT 1 cut(s) 1099
NspI RCATGY 2 cut(s) 722, 887
PaeR7I CTCGAG 1 cut(s) 338
PagI TCATGA 1 cut(s) 408
PceI AGGCCT 1 cut(s) 622
PciI ACATGT 1 cut(s) 883
PcsI WCGNNNNNNNCGW 5 cut(s) 75, 87, 180, 399, 1073
PfeI GAWTC 1 cut(s) 1072
PkrI GCNGC 9 cut(s) 311, 348, 361, 371, 374, 531, 552, 613, 806
PleI GAGTC 2 cut(s) 589, 677
PluTI GGCGCC 1 cut(s) 794
PpsI GAGTC 2 cut(s) 589, 677
PpuMI RGGWCCY 1 cut(s) 535
PscI ACATGT 1 cut(s) 883
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 535
Psp6I CCWGG 2 cut(s) 282, 901
PspFI CCCAGC 3 cut(s) 25, 46, 1112
PspGI CCWGG 2 cut(s) 282, 901
PspN4I GGNNCC 6 cut(s) 268, 376, 536, 537, 792, 1061
PspPI GGNCC 7 cut(s) 163, 258, 280, 535, 600, 629, 1148
PspPPI RGGWCCY 1 cut(s) 535
PstI CTGCAG 1 cut(s) 589
PsuI RGATCY 3 cut(s) 266, 324, 1059
RsaI GTAC 2 cut(s) 434, 1082
RsaNI GTAC 2 cut(s) 433, 1081
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 321
SacII CCGCGG 2 cut(s) 533, 808
SalI GTCGAC 2 cut(s) 68, 80
SaqAI TTAA 2 cut(s) 723, 768
SatI GCNGC 9 cut(s) 310, 347, 360, 370, 373, 530, 551, 612, 805
Sau3AI GATC 6 cut(s) 266, 324, 405, 918, 948, 1059
Sau96I GGNCC 7 cut(s) 163, 258, 280, 535, 600, 629, 1148
SchI GAGTC 2 cut(s) 590, 677
ScrFI CCNGG 2 cut(s) 284, 903
SduI GDGCHC 3 cut(s) 300, 321, 475
SfaNI GCATC 3 cut(s) 38, 356, 1106
SfcI CTRYAG 1 cut(s) 585
SfoI GGCGCC 1 cut(s) 792
Sfr274I CTCGAG 1 cut(s) 338
Sfr303I CCGCGG 2 cut(s) 533, 808
SgrBI CCGCGG 2 cut(s) 533, 808
SinI GGWCC 5 cut(s) 163, 280, 535, 629, 1148
SlaI CTCGAG 1 cut(s) 338
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 320, 338
SmoI CTYRAG 2 cut(s) 320, 338
SpeI ACTAGT 1 cut(s) 638
Sse9I AATT 3 cut(s) 466, 606, 1045
SseBI AGGCCT 1 cut(s) 622
SspDI GGCGCC 1 cut(s) 790
SspMI CTAG 2 cut(s) 639, 734
SstI GAGCTC 1 cut(s) 321
StuI AGGCCT 1 cut(s) 622
StyD4I CCNGG 2 cut(s) 282, 901
StyI CCWWGG 2 cut(s) 441, 999
TaaI ACNGT 5 cut(s) 167, 277, 305, 379, 667
TaiI ACGT 2 cut(s) 297, 1070
TaqI TCGA 7 cut(s) 69, 81, 300, 339, 543, 1049, 1070
TasI AATT 3 cut(s) 466, 606, 1045
TauI GCSGC 7 cut(s) 312, 362, 372, 375, 532, 614, 807
TfiI GAWTC 1 cut(s) 1072
Tru1I TTAA 2 cut(s) 723, 768
Tru9I TTAA 2 cut(s) 723, 768
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 2 cut(s) 91, 661
TseI GCWGC 2 cut(s) 346, 550
Tsp45I GTSAC 2 cut(s) 91, 661
TspDTI ATGAA 4 cut(s) 425, 515, 758, 861
TspGWI ACGGA 2 cut(s) 79, 1065
TspRI CASTG 1 cut(s) 670
VpaK11BI GGWCC 5 cut(s) 163, 280, 535, 629, 1148
VspI ATTAAT 1 cut(s) 768
XapI RAATTY 1 cut(s) 1045
XceI RCATGY 2 cut(s) 722, 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XhoI CTCGAG 1 cut(s) 338
XmiI GTMKAC 3 cut(s) 69, 81, 482
XspI CTAG 2 cut(s) 639, 734
ZraI GACGTC 1 cut(s) 1068
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.