Rroxscaffold_5G00376970

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014204
Physical Location & Seq
Forward (+)
57538034 .. 57539608
1575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00376970.1

Sequence Viewer

Length: 1095 bp
ATGTCAAATAATTTGCCACGCATGCCAAACACAAAAAATAGAATGCAACAGATCAGCAACAGATGTAAAATACATGCCAAGGCCCGTCTGATCGCGATCGGCGATGTCCACGGCGACCTCGACAAGACCAAGCAATCACTACGCCTCGCCAATCTGATCGATCAGTCCGACAAATGGGTCGGAGGGTCCGCCACCGTGGTCCAGGTCGGCGACGTGCTCGACCGCGGCGGCGACGAGATCAGAATCCTGTATTTCCTGGAGAAGCTGAGACGGGAAGCCGCCAGATGCGGCGGCAGCTTGATCACCATGCACGGCAACCACGAGATTATGAACGTGGAAGGAGATTTCAGGTACGTGACCAGAGCGGGTTTGGATGAGTTTCTGAATTGGGCGGATTGGTACACAGTTGGGAACAGAATGAAAGCCCTCTGTAAAGGGTTGGAAAACCCTAAAGACCCATTTGAGGGGCTGCCTTGGGGTTTCAAAAATGTCAAGAAAGAGTTCACACAAGGTTTTAGGGCCAGGATCGCCGCGTTGAGGCCCGATGGTCCCATCACCCGTCGGTTCTTGTCCAAGAATGTGACTGTGTTGGTTGTGGGGGACTCGGTTTTCGTGCATGGCGGCCTTTTGGCTAAGCATGTTTCGTATGGTCTGGAGAAGATGAATGAGGAGGTGAGGGAGTGGGTTTGTGGGGTGAAGGGCAGATTGGCACCTGAGTATTGCCGTGGGGGCAACTCTGTGGTTTGGTTGAGGAAGTTTTCTGACGAATTTGCGGACAGGTGTGATTGTTCGGCACTGCAGCATGTGCTGGAAACTATTCCTGGTGCTAAGAGGATGATCATGGGGCATACCATTCAACAGTTTGGGATTAATGGCGCTTGCGATGAGAGAGCTATTCGGATTGACGTGGGGATGTCCAAGGGCTGTGGTGATGGCTTGCCTGAAGTGTTGGAGATCAATGGGAATTCGAGTTTGAGGATCCTGACGTCGAATCCGTTGTACCAGAAGAAGTACAAATCTCCTGCTGAAGCTGCGAGTAGGGAAGGGGTTGGATTTTTGGAGCAGGGACCAAGACAAGTGCAAGTGAAGGCTTGA

Protein Analysis

364

Amino Acids

40.57

Weight (kDa)

9.22

Isoelectric Point (pI)

30.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 29 - 141 1.8e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18480
fragaria_vesca FvH4_3g25460 FvH4_3g25460 FvH4_3g25460
malus_domestica MD02G1292100.v1.1 MD07G1034500.v1.1
prunus_persica Prupe.2G031200_v2.0.a1
pyrus_communis pycom02g24670
rosa_chinensis RchiOBHm_Chr4g0435781
rosa_laevigata RLG00000006559
rosa_multiflora Rmu_sc0000051.1_g000023 Rmu_sc0004389.1_g000004
rosa_roxburghii Rroxscaffold_5G00376970
rosa_rugosa Rorug04G0289600
rosa_samantha Rh4BG352600 Rh4CG367100
rosa_wichuraiana Rw4G030030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 176
AatII GACGTC 1 cut(s) 989
AccB1I GGYRCC 1 cut(s) 709
AccBSI CCGCTC 1 cut(s) 365
AccII CGCG 3 cut(s) 95, 225, 533
AclWI GGATC 3 cut(s) 533, 973, 986
AcsI RAATTY 2 cut(s) 767, 964
AcuI CTGAAG 2 cut(s) 963, 1047
AcyI GRCGYC 1 cut(s) 986
AfaI GTAC 4 cut(s) 353, 401, 1001, 1013
AfiI CCNNNNNNNGG 4 cut(s) 174, 463, 464, 537
AgsI TTSAA 2 cut(s) 484, 857
AjiI CACGTC 2 cut(s) 214, 907
AjnI CCWGG 4 cut(s) 201, 255, 521, 820
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
AluBI AGCT 4 cut(s) 265, 297, 893, 1031
AluI AGCT 4 cut(s) 265, 297, 893, 1031
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 1 cut(s) 262
AlwI GGATC 3 cut(s) 533, 973, 986
AoxI GGCC 4 cut(s) 81, 519, 539, 622
ApeKI GCWGC 4 cut(s) 294, 469, 799, 1031
ApoI RAATTY 2 cut(s) 767, 964
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 2 cut(s) 500, 816
AspLEI GCGC 1 cut(s) 878
AspS9I GGNCC 7 cut(s) 82, 186, 199, 519, 540, 548, 1067
AsuHPI GGTGA 5 cut(s) 295, 547, 685, 706, 941
AvaII GGWCC 4 cut(s) 186, 199, 548, 1067
BamHI GGATCC 1 cut(s) 978
BanI GGYRCC 1 cut(s) 709
BauI CACGAG 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 4 cut(s) 306, 456, 811, 1018
BccI CCATC 3 cut(s) 539, 560, 926
BceAI ACGGC 3 cut(s) 127, 328, 708
BciT130I CCWGG 4 cut(s) 203, 257, 523, 822
BclI TGATCA 2 cut(s) 300, 837
BcoDI GTCTC 1 cut(s) 262
BfmI CTRYAG 1 cut(s) 797
BfoI RGCGCY 1 cut(s) 879
BglI GCCNNNNNGGC 1 cut(s) 729
BlpI GCTNAGC 1 cut(s) 633
Bme1390I CCNGG 4 cut(s) 203, 257, 523, 822
Bme18I GGWCC 4 cut(s) 186, 199, 548, 1067
BmgBI CACGTC 2 cut(s) 214, 907
BmgT120I GGNCC 7 cut(s) 82, 186, 199, 519, 540, 548, 1067
BmiI GGNNCC 5 cut(s) 187, 550, 711, 980, 1068
BmrFI CCNGG 4 cut(s) 203, 257, 523, 822
BmsI GCATC 1 cut(s) 275
BpmI CTGGAG 2 cut(s) 278, 674
Bpu1102I GCTNAGC 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 159
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 2 cut(s) 95, 242
BsaHI GRCGYC 1 cut(s) 986
BsaJI CCNNGG 7 cut(s) 78, 109, 195, 223, 473, 724, 918
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 463, 464, 537
Bse8I GATNNNNATC 2 cut(s) 95, 242
BseBI CCWGG 4 cut(s) 203, 257, 523, 822
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 7 cut(s) 78, 109, 195, 223, 473, 724, 918
BseGI GGATG 3 cut(s) 379, 840, 918
BseJI GATNNNNATC 2 cut(s) 95, 242
BseLI CCNNNNNNNGG 4 cut(s) 174, 463, 464, 537
BseMII CTCAG 2 cut(s) 257, 705
BseRI GAGGAG 1 cut(s) 683
BseXI GCAGC 4 cut(s) 306, 456, 811, 1018
Bsh1236I CGCG 3 cut(s) 95, 225, 533
Bsh1285I CGRYCG 2 cut(s) 99, 223
BshFI GGCC 4 cut(s) 83, 521, 541, 624
BshNI GGYRCC 1 cut(s) 709
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 2 cut(s) 99, 223
BsiHKAI GWGCWC 1 cut(s) 219
BslFI GGGAC 3 cut(s) 534, 614, 1080
BslI CCNNNNNNNGG 4 cut(s) 174, 463, 464, 537
BsmAI GTCTC 1 cut(s) 262
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 3 cut(s) 534, 614, 1080
BsmI GAATGC 1 cut(s) 48
BsnI GGCC 4 cut(s) 83, 521, 541, 624
Bsp1286I GDGCHC 1 cut(s) 219
Bsp1720I GCTNAGC 1 cut(s) 633
Bsp68I TCGCGA 1 cut(s) 95
BspANI GGCC 4 cut(s) 83, 521, 541, 624
BspCNI CTCAG 2 cut(s) 258, 706
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 3 cut(s) 95, 225, 533
BspLI GGNNCC 5 cut(s) 187, 550, 711, 980, 1068
BspMAI CTGCAG 1 cut(s) 801
BspPI GGATC 3 cut(s) 533, 973, 986
BspT107I GGYRCC 1 cut(s) 709
BsrBI CCGCTC 1 cut(s) 365
BssECI CCNNGG 7 cut(s) 78, 109, 195, 223, 473, 724, 918
BssNI GRCGYC 1 cut(s) 986
BssSI CACGAG 1 cut(s) 320
BssT1I CCWWGG 3 cut(s) 78, 473, 918
Bst2BI CACGAG 1 cut(s) 320
Bst2UI CCWGG 4 cut(s) 203, 257, 523, 822
Bst4CI ACNGT 4 cut(s) 196, 406, 586, 861
BstACI GRCGYC 1 cut(s) 986
BstAPI GCANNNNNTGC 1 cut(s) 805
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 23, 880, 938
BstDEI CTNAG 4 cut(s) 266, 633, 714, 828
BstDSI CCRYGG 4 cut(s) 109, 195, 223, 724
BstF5I GGATG 3 cut(s) 379, 840, 918
BstFNI CGCG 3 cut(s) 95, 225, 533
BstH2I RGCGCY 1 cut(s) 879
BstHHI GCGC 1 cut(s) 878
BstMAI GTCTC 1 cut(s) 262
BstMCI CGRYCG 2 cut(s) 99, 223
BstMWI GCNNNNNNNGC 6 cut(s) 22, 294, 527, 729, 805, 1031
BstNI CCWGG 4 cut(s) 203, 257, 523, 822
BstNSI RCATGY 4 cut(s) 25, 77, 641, 806
BstSCI CCNGG 4 cut(s) 201, 255, 521, 820
BstSFI CTRYAG 1 cut(s) 797
BstUI CGCG 3 cut(s) 95, 225, 533
BstV1I GCAGC 4 cut(s) 306, 456, 811, 1018
BstX2I RGATCY 1 cut(s) 978
BstYI RGATCY 1 cut(s) 978
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 4 cut(s) 83, 521, 541, 624
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 4 cut(s) 109, 195, 223, 724
BtgZI GCGATG 2 cut(s) 117, 897
BtrI CACGTC 2 cut(s) 214, 907
BtsCI GGATG 3 cut(s) 379, 840, 918
BtsI GCAGTG 1 cut(s) 794
BtsIMutI CAGTG 1 cut(s) 794
BtuMI TCGCGA 1 cut(s) 95
Cac8I GCNNGC 3 cut(s) 23, 880, 938
CfoI GCGC 1 cut(s) 878
Cfr13I GGNCC 7 cut(s) 82, 186, 199, 519, 540, 548, 1067
Cfr42I CCGCGG 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 4 cut(s) 352, 400, 1000, 1012
CspCI CAANNNNNGTGG 2 cut(s) 907, 942
CviAII CATG 7 cut(s) 22, 74, 307, 617, 638, 803, 841
CviQI GTAC 4 cut(s) 352, 400, 1000, 1012
DdeI CTNAG 4 cut(s) 266, 633, 714, 828
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EciI GGCGGA 2 cut(s) 178, 407
Eco130I CCWWGG 3 cut(s) 78, 473, 918
Eco47I GGWCC 4 cut(s) 186, 199, 548, 1067
Eco57I CTGAAG 2 cut(s) 963, 1047
EcoRI GAATTC 1 cut(s) 964
EcoRII CCWGG 4 cut(s) 201, 255, 521, 820
EcoT14I CCWWGG 3 cut(s) 78, 473, 918
ErhI CCWWGG 3 cut(s) 78, 473, 918
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 7 cut(s) 25, 77, 310, 620, 641, 806, 844
FaqI GGGAC 3 cut(s) 534, 614, 1080
FatI CATG 7 cut(s) 21, 73, 306, 616, 637, 802, 840
FauI CCCGC 1 cut(s) 358
FbaI TGATCA 2 cut(s) 300, 837
FokI GGATG 3 cut(s) 386, 847, 925
GlaI GCGC 1 cut(s) 877
GsuI CTGGAG 2 cut(s) 278, 674
HaeII RGCGCY 1 cut(s) 879
HaeIII GGCC 4 cut(s) 83, 521, 541, 624
HhaI GCGC 1 cut(s) 878
Hin1I GRCGYC 1 cut(s) 986
Hin1II CATG 7 cut(s) 25, 77, 310, 620, 641, 806, 844
Hin6I GCGC 1 cut(s) 876
HinP1I GCGC 1 cut(s) 876
HinfI GANTC 3 cut(s) 243, 602, 991
HphI GGTGA 5 cut(s) 295, 547, 685, 706, 941
Hpy166II GTNNAC 3 cut(s) 109, 402, 504
Hpy188I TCNGA 8 cut(s) 90, 156, 169, 182, 242, 384, 763, 900
Hpy188III TCNNGA 4 cut(s) 94, 493, 653, 982
Hpy8I GTNNAC 3 cut(s) 109, 402, 504
Hpy99I CGWCG 4 cut(s) 215, 236, 564, 991
HpyAV CCTTC 4 cut(s) 332, 691, 1037, 1081
HpyCH4III ACNGT 4 cut(s) 196, 406, 586, 861
HpyCH4IV ACGT 5 cut(s) 213, 333, 354, 906, 986
HpyCH4V TGCA 5 cut(s) 46, 310, 616, 799, 1081
HpyF10VI GCNNNNNNNGC 6 cut(s) 22, 294, 527, 729, 805, 1031
HpyF3I CTNAG 4 cut(s) 266, 633, 714, 828
HpySE526I ACGT 5 cut(s) 213, 333, 354, 906, 986
Hsp92I GRCGYC 1 cut(s) 986
Hsp92II CATG 7 cut(s) 25, 77, 310, 620, 641, 806, 844
HspAI GCGC 1 cut(s) 876
Ksp22I TGATCA 2 cut(s) 300, 837
KspI CCGCGG 1 cut(s) 226
LmnI GCTCC 1 cut(s) 1060
Lsp1109I GCAGC 4 cut(s) 306, 456, 811, 1018
LweI GCATC 1 cut(s) 275
MaeII ACGT 5 cut(s) 213, 333, 354, 906, 986
MaeIII GTNAC 2 cut(s) 355, 580
MbiI CCGCTC 1 cut(s) 365
MboII GAAGA 2 cut(s) 670, 1018
MflI RGATCY 1 cut(s) 978
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 4 cut(s) 10, 385, 767, 964
MlyI GAGTC 1 cut(s) 596
MmeI TCCRAC 5 cut(s) 160, 192, 420, 930, 1030
MroXI GAANNNNTTC 2 cut(s) 500, 816
MseI TTAA 1 cut(s) 870
MspA1I CMGCKG 1 cut(s) 225
MspR9I CCNGG 4 cut(s) 203, 257, 523, 822
Mva1269I GAATGC 1 cut(s) 48
MvaI CCWGG 4 cut(s) 203, 257, 523, 822
MvnI CGCG 3 cut(s) 95, 225, 533
MwoI GCNNNNNNNGC 6 cut(s) 22, 294, 527, 729, 805, 1031
NlaIII CATG 7 cut(s) 25, 77, 310, 620, 641, 806, 844
NlaIV GGNNCC 5 cut(s) 187, 550, 711, 980, 1068
NmuCI GTSAC 2 cut(s) 355, 580
NruI TCGCGA 1 cut(s) 95
NspI RCATGY 4 cut(s) 25, 77, 641, 806
PaeI GCATGC 1 cut(s) 25
PcsI WCGNNNNNNNCGW 5 cut(s) 99, 117, 165, 318, 992
PctI GAATGC 1 cut(s) 48
PdmI GAANNNNTTC 2 cut(s) 500, 816
PfeI GAWTC 2 cut(s) 243, 991
PfoI TCCNGGA 1 cut(s) 255
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 1 cut(s) 596
PpsI GAGTC 1 cut(s) 596
Ppu21I YACGTR 1 cut(s) 355
PshBI ATTAAT 1 cut(s) 870
Psp6I CCWGG 4 cut(s) 201, 255, 521, 820
PspGI CCWGG 4 cut(s) 201, 255, 521, 820
PspN4I GGNNCC 5 cut(s) 187, 550, 711, 980, 1068
PspPI GGNCC 7 cut(s) 82, 186, 199, 519, 540, 548, 1067
PstI CTGCAG 1 cut(s) 801
PsuI RGATCY 1 cut(s) 978
PvuI CGATCG 1 cut(s) 99
RruI TCGCGA 1 cut(s) 95
RsaI GTAC 4 cut(s) 353, 401, 1001, 1013
RsaNI GTAC 4 cut(s) 352, 400, 1000, 1012
SacII CCGCGG 1 cut(s) 226
SaqAI TTAA 1 cut(s) 870
Sau96I GGNCC 7 cut(s) 82, 186, 199, 519, 540, 548, 1067
SchI GAGTC 1 cut(s) 596
ScrFI CCNGG 4 cut(s) 203, 257, 523, 822
SduI GDGCHC 1 cut(s) 219
SfaNI GCATC 1 cut(s) 275
SfcI CTRYAG 1 cut(s) 797
Sfr303I CCGCGG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 226
SinI GGWCC 4 cut(s) 186, 199, 548, 1067
SphI GCATGC 1 cut(s) 25
Sse9I AATT 4 cut(s) 10, 385, 767, 964
StyD4I CCNGG 4 cut(s) 201, 255, 521, 820
StyI CCWWGG 3 cut(s) 78, 473, 918
TaaI ACNGT 4 cut(s) 196, 406, 586, 861
TaiI ACGT 5 cut(s) 216, 336, 357, 909, 989
TaqI TCGA 5 cut(s) 120, 159, 219, 968, 989
TasI AATT 4 cut(s) 10, 385, 767, 964
TatI WGTACW 1 cut(s) 1011
TauI GCSGC 7 cut(s) 228, 231, 281, 291, 294, 533, 624
TfiI GAWTC 2 cut(s) 243, 991
Tru1I TTAA 1 cut(s) 870
Tru9I TTAA 1 cut(s) 870
TscAI CASTG 1 cut(s) 801
TseFI GTSAC 2 cut(s) 355, 580
TseI GCWGC 4 cut(s) 294, 469, 799, 1031
Tsp45I GTSAC 2 cut(s) 355, 580
TspDTI ATGAA 3 cut(s) 344, 434, 677
TspGWI ACGGA 1 cut(s) 984
TspRI CASTG 1 cut(s) 801
VpaK11BI GGWCC 4 cut(s) 186, 199, 548, 1067
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 2 cut(s) 767, 964
XceI RCATGY 4 cut(s) 25, 77, 641, 806
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmnI GAANNNNTTC 2 cut(s) 500, 816
ZraI GACGTC 1 cut(s) 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.