RLG00000006559

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014204
Physical Location & Seq
Reverse (-)
8488211 .. 8489431
1221 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006559

Sequence Viewer

Length: 1221 bp
ATGGCAGACGTGGACCTGAAAGCGAAGAAGAGCTCAGCGTCAGAGTCATGCAAAGACGTTCCATCTCTCCTCACCTCTTTCGTCGACACCTTCGTCGACTTCACGGTCAGTGGACATTTCCTCTTACCCGAAAACCCCATTCCACTTCCCCAAAATTACACCAACGCCACTACTACTCCGCCGCTGCAAACCTTCTACCCACCGCAGGACCGTCTGATCGCGATCGGCGATGTCCACGGCGACCTCGACAAGACCAAGCAATCACTACGCCTCGCCAATCTGATCGATCACTCCGACAAATGGGTCGGAGGGTCCGCCACCGTTGTCCAGGTCGGCGACGTGCTCGACCGCGGCGGCGACGAGATCAAAATCCTGTATTTCCTCGAGAAGCTGAGACGGGAAGCCGCCAGGTGCGGCGGCAGCTTGATCACCATGCACGGCAACCACGAGATTATGAACGTGGAAGGAGATTTCAGGTACGTGACCAGAGAGGGTTTGGATGAGTTTCTGAATTGGGCTCATTGGTACAGAGTTGGCAACAGAATGAAAGCCCTTTGTAAAGGGTTGGAAAACCCTAAAGACCCATTTGAGGGGCTGCCTTTGGGTTTCAAAAATGTCAAGAAAGAGTTCACAGAAGGTTTCAGGGCCAGGATCGCCGCGCTGAGGCCTGATGGTCCGATCGCCGGTCGGTTCCTGTCCAAGAATGTGACTGTGTTGGTTGTGGGGGACTCGGTTTTCGTCCATGGCGGGCTTTTGGCTAAGCATGTTTCGTATGGTCTGGAGAAGATGAATGAGGAGGTGAGGGAGTGGGTCAATGGGGTGAAGGGGAGATTGGCACCTGAGTATTGCCGTGGGAGGAACTCTGTGGTTTGGTTGAGGAAGTTTTCTGATGAATTTGCGGACAGGTGTGATTGTTCGGCACTGCAACATGTGCTGCAAACTATTCCTGGTGCTAAGAGGATGATCATGGGTCATACCATTCAACAGTTTGGGATTAATGGCGCTTGCGATGAGAGAGCTATTCGGATTGACGTGGGGATGTCCAAGGGCTGTGGTGATGGCTTGCCTGAAGTCTTGGAGATCAATGGGAATTCGAGTTTGAGGATCCTGACATCGAATTCGTTGTACCAGAAGAAGTATAAATCTCCTGCTGAAGCTGGGAGTAGGGAAGGGGTTGGATTTTTGGAGCAGGGACCAAGACAAGTGCAAGTGAAGGCTTGA

Protein Analysis

407

Amino Acids

44.96

Weight (kDa)

8.04

Isoelectric Point (pI)

30.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 71 - 326 1.1e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18480
fragaria_vesca FvH4_3g25460 FvH4_3g25460 FvH4_3g25460
malus_domestica MD02G1292100.v1.1 MD07G1034500.v1.1
prunus_persica Prupe.2G031200_v2.0.a1
pyrus_communis pycom02g24670
rosa_chinensis RchiOBHm_Chr4g0435781
rosa_laevigata RLG00000006559
rosa_multiflora Rmu_sc0000051.1_g000023 Rmu_sc0004389.1_g000004
rosa_roxburghii Rroxscaffold_5G00376970
rosa_rugosa Rorug04G0289600
rosa_samantha Rh4BG352600 Rh4CG367100
rosa_wichuraiana Rw4G030030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 92, 104, 302
AccB1I GGYRCC 1 cut(s) 835
AccI GTMKAC 2 cut(s) 84, 96
AccII CGCG 3 cut(s) 221, 351, 659
AclWI GGATC 3 cut(s) 659, 1099, 1112
AcsI RAATTY 3 cut(s) 893, 1090, 1117
AcuI CTGAAG 2 cut(s) 1089, 1173
AfaI GTAC 3 cut(s) 479, 527, 1127
AfiI CCNNNNNNNGG 6 cut(s) 205, 300, 589, 590, 663, 683
AflIII ACRYGT 1 cut(s) 928
AgsI TTSAA 2 cut(s) 610, 983
AjiI CACGTC 3 cut(s) 10, 340, 1033
AjnI CCWGG 4 cut(s) 327, 407, 647, 946
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AluBI AGCT 5 cut(s) 33, 391, 423, 1019, 1157
AluI AGCT 5 cut(s) 33, 391, 423, 1019, 1157
Alw21I GWGCWC 2 cut(s) 35, 345
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 3 cut(s) 659, 1099, 1112
Ama87I CYCGRG 1 cut(s) 383
AoxI GGCC 2 cut(s) 645, 665
ApeKI GCWGC 4 cut(s) 184, 420, 595, 934
ApoI RAATTY 3 cut(s) 893, 1090, 1117
ArsI GACNNNNNNTTYG 4 cut(s) 719, 751, 1102, 1134
AseI ATTAAT 1 cut(s) 996
Asp700I GAANNNNTTC 1 cut(s) 626
AspLEI GCGC 2 cut(s) 661, 1004
AspS9I GGNCC 6 cut(s) 13, 208, 312, 645, 674, 1193
AsuHPI GGTGA 5 cut(s) 64, 421, 811, 832, 1067
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 5 cut(s) 13, 208, 312, 674, 1193
BamHI GGATCC 1 cut(s) 1104
BanI GGYRCC 1 cut(s) 835
BanII GRGCYC 2 cut(s) 35, 520
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 2 cut(s) 35, 345
BbvCI CCTCAGC 1 cut(s) 662
BbvI GCAGC 4 cut(s) 171, 432, 582, 921
BccI CCATC 3 cut(s) 70, 665, 1052
BceAI ACGGC 3 cut(s) 253, 454, 834
BciT130I CCWGG 4 cut(s) 329, 409, 649, 948
BclI TGATCA 2 cut(s) 426, 963
BcoDI GTCTC 1 cut(s) 388
BfoI RGCGCY 1 cut(s) 1005
BlpI GCTNAGC 2 cut(s) 34, 759
Bme1390I CCNGG 4 cut(s) 329, 409, 649, 948
Bme18I GGWCC 5 cut(s) 13, 208, 312, 674, 1193
BmeT110I CYCGRG 1 cut(s) 383
BmgBI CACGTC 3 cut(s) 10, 340, 1033
BmgT120I GGNCC 6 cut(s) 13, 208, 312, 645, 674, 1193
BmiI GGNNCC 5 cut(s) 313, 692, 837, 1106, 1194
BmrFI CCNGG 4 cut(s) 329, 409, 649, 948
BpmI CTGGAG 1 cut(s) 800
Bpu10I CCTNAGC 1 cut(s) 662
Bpu1102I GCTNAGC 2 cut(s) 34, 759
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 2 cut(s) 221, 368
BsaJI CCNNGG 5 cut(s) 235, 349, 742, 850, 1044
BsaXI ACNNNNNCTCC 2 cut(s) 160, 190
Bsc4I CCNNNNNNNGG 6 cut(s) 205, 300, 589, 590, 663, 683
Bse118I RCCGGY 1 cut(s) 683
Bse8I GATNNNNATC 2 cut(s) 221, 368
BseBI CCWGG 4 cut(s) 329, 409, 649, 948
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 5 cut(s) 235, 349, 742, 850, 1044
BseGI GGATG 3 cut(s) 505, 966, 1044
BseJI GATNNNNATC 2 cut(s) 221, 368
BseLI CCNNNNNNNGG 6 cut(s) 205, 300, 589, 590, 663, 683
BseMII CTCAG 4 cut(s) 48, 383, 653, 831
BseRI GAGGAG 2 cut(s) 59, 809
BseXI GCAGC 4 cut(s) 171, 432, 582, 921
BseYI CCCAGC 1 cut(s) 1157
Bsh1236I CGCG 3 cut(s) 221, 351, 659
Bsh1285I CGRYCG 4 cut(s) 225, 349, 681, 688
BshFI GGCC 2 cut(s) 647, 667
BshNI GGYRCC 1 cut(s) 835
BshVI ATCGAT 1 cut(s) 285
BsiEI CGRYCG 4 cut(s) 225, 349, 681, 688
BsiHKAI GWGCWC 2 cut(s) 35, 345
BsiHKCI CYCGRG 1 cut(s) 383
BsiSI CCGG 1 cut(s) 684
BslFI GGGAC 2 cut(s) 740, 1206
BslI CCNNNNNNNGG 6 cut(s) 205, 300, 589, 590, 663, 683
BsmAI GTCTC 1 cut(s) 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 2 cut(s) 740, 1206
BsnI GGCC 2 cut(s) 647, 667
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 3 cut(s) 35, 345, 520
Bsp1720I GCTNAGC 2 cut(s) 34, 759
Bsp19I CCATGG 1 cut(s) 742
Bsp68I TCGCGA 1 cut(s) 221
BspANI GGCC 2 cut(s) 647, 667
BspCNI CTCAG 4 cut(s) 47, 384, 654, 832
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 3 cut(s) 221, 351, 659
BspLI GGNNCC 5 cut(s) 313, 692, 837, 1106, 1194
BspPI GGATC 3 cut(s) 659, 1099, 1112
BspQI GCTCTTC 1 cut(s) 23
BspT107I GGYRCC 1 cut(s) 835
BsrFI RCCGGY 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 5 cut(s) 235, 349, 742, 850, 1044
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 2 cut(s) 742, 1044
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 4 cut(s) 329, 409, 649, 948
Bst4CI ACNGT 5 cut(s) 106, 212, 322, 712, 987
Bst6I CTCTTC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 931
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 749, 1006, 1064
BstDEI CTNAG 6 cut(s) 34, 392, 662, 759, 840, 954
BstDSI CCRYGG 4 cut(s) 235, 349, 742, 850
BstF5I GGATG 3 cut(s) 505, 966, 1044
BstFNI CGCG 3 cut(s) 221, 351, 659
BstH2I RGCGCY 1 cut(s) 1005
BstHHI GCGC 2 cut(s) 661, 1004
BstMAI GTCTC 1 cut(s) 388
BstMCI CGRYCG 4 cut(s) 225, 349, 681, 688
BstMWI GCNNNNNNNGC 3 cut(s) 420, 653, 931
BstNI CCWGG 4 cut(s) 329, 409, 649, 948
BstNSI RCATGY 2 cut(s) 767, 932
BstSCI CCNGG 4 cut(s) 327, 407, 647, 946
BstUI CGCG 3 cut(s) 221, 351, 659
BstV1I GCAGC 4 cut(s) 171, 432, 582, 921
BstX2I RGATCY 1 cut(s) 1104
BstYI RGATCY 1 cut(s) 1104
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 2 cut(s) 647, 667
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 4 cut(s) 235, 349, 742, 850
BtgZI GCGATG 2 cut(s) 243, 1023
BtrI CACGTC 3 cut(s) 10, 340, 1033
BtsCI GGATG 3 cut(s) 505, 966, 1044
BtsI GCAGTG 1 cut(s) 920
BtsIMutI CAGTG 2 cut(s) 115, 920
BtuMI TCGCGA 1 cut(s) 221
Cac8I GCNNGC 3 cut(s) 749, 1006, 1064
CfoI GCGC 2 cut(s) 661, 1004
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 6 cut(s) 13, 208, 312, 645, 674, 1193
Cfr42I CCGCGG 1 cut(s) 352
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 27
Csp6I GTAC 3 cut(s) 478, 526, 1126
CspCI CAANNNNNGTGG 2 cut(s) 1033, 1068
CviAII CATG 6 cut(s) 48, 433, 743, 764, 929, 967
CviQI GTAC 3 cut(s) 478, 526, 1126
DdeI CTNAG 6 cut(s) 34, 392, 662, 759, 840, 954
DrdI GACNNNNNNGTC 3 cut(s) 92, 104, 302
DseDI GACNNNNNNGTC 3 cut(s) 92, 104, 302
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 2 cut(s) 168, 304
Ecl136II GAGCTC 1 cut(s) 33
Eco130I CCWWGG 2 cut(s) 742, 1044
Eco147I AGGCCT 1 cut(s) 667
Eco24I GRGCYC 2 cut(s) 35, 520
Eco47I GGWCC 5 cut(s) 13, 208, 312, 674, 1193
Eco53kI GAGCTC 1 cut(s) 33
Eco57I CTGAAG 2 cut(s) 1089, 1173
Eco88I CYCGRG 1 cut(s) 383
EcoICRI GAGCTC 1 cut(s) 33
EcoRI GAATTC 2 cut(s) 1090, 1117
EcoRII CCWGG 4 cut(s) 327, 407, 647, 946
EcoT14I CCWWGG 2 cut(s) 742, 1044
EcoT38I GRGCYC 2 cut(s) 35, 520
ErhI CCWWGG 2 cut(s) 742, 1044
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 6 cut(s) 51, 436, 746, 767, 932, 970
FaqI GGGAC 2 cut(s) 740, 1206
FatI CATG 6 cut(s) 47, 432, 742, 763, 928, 966
FauI CCCGC 1 cut(s) 740
FbaI TGATCA 2 cut(s) 426, 963
FblI GTMKAC 2 cut(s) 84, 96
FokI GGATG 3 cut(s) 512, 973, 1051
FriOI GRGCYC 2 cut(s) 35, 520
GlaI GCGC 2 cut(s) 660, 1003
GsaI CCCAGC 1 cut(s) 1161
GsuI CTGGAG 1 cut(s) 800
HaeII RGCGCY 1 cut(s) 1005
HaeIII GGCC 2 cut(s) 647, 667
HapII CCGG 1 cut(s) 684
HgaI GACGC 1 cut(s) 27
HhaI GCGC 2 cut(s) 661, 1004
Hin1II CATG 6 cut(s) 51, 436, 746, 767, 932, 970
Hin6I GCGC 2 cut(s) 659, 1002
HinP1I GCGC 2 cut(s) 659, 1002
HincII GTYRAC 2 cut(s) 85, 97
HindII GTYRAC 2 cut(s) 85, 97
HinfI GANTC 2 cut(s) 44, 728
HpaII CCGG 1 cut(s) 684
HphI GGTGA 5 cut(s) 64, 421, 811, 832, 1067
Hpy166II GTNNAC 6 cut(s) 13, 85, 97, 113, 235, 630
Hpy188I TCNGA 9 cut(s) 43, 216, 282, 295, 308, 510, 678, 889, 1026
Hpy188III TCNNGA 5 cut(s) 220, 385, 619, 779, 1108
Hpy8I GTNNAC 6 cut(s) 13, 85, 97, 113, 235, 630
Hpy99I CGWCG 4 cut(s) 86, 98, 341, 362
HpyAV CCTTC 7 cut(s) 100, 202, 458, 629, 817, 1163, 1207
HpyCH4III ACNGT 5 cut(s) 106, 212, 322, 712, 987
HpyCH4IV ACGT 6 cut(s) 9, 57, 339, 459, 480, 1032
HpyCH4V TGCA 6 cut(s) 51, 187, 436, 925, 937, 1207
HpyF10VI GCNNNNNNNGC 3 cut(s) 420, 653, 931
HpyF3I CTNAG 6 cut(s) 34, 392, 662, 759, 840, 954
HpySE526I ACGT 6 cut(s) 9, 57, 339, 459, 480, 1032
Hsp92II CATG 6 cut(s) 51, 436, 746, 767, 932, 970
HspAI GCGC 2 cut(s) 659, 1002
Ksp22I TGATCA 2 cut(s) 426, 963
KspI CCGCGG 1 cut(s) 352
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 1186
Lsp1109I GCAGC 4 cut(s) 171, 432, 582, 921
MaeII ACGT 6 cut(s) 9, 57, 339, 459, 480, 1032
MaeIII GTNAC 2 cut(s) 481, 706
MboII GAAGA 4 cut(s) 37, 40, 796, 1144
MflI RGATCY 1 cut(s) 1104
MhlI GDGCHC 3 cut(s) 35, 345, 520
MluCI AATT 5 cut(s) 154, 511, 893, 1090, 1117
MlyI GAGTC 2 cut(s) 53, 722
MmeI TCCRAC 4 cut(s) 286, 318, 546, 1156
MroXI GAANNNNTTC 1 cut(s) 626
MseI TTAA 1 cut(s) 996
MspA1I CMGCKG 2 cut(s) 184, 351
MspI CCGG 1 cut(s) 684
MspR9I CCNGG 4 cut(s) 329, 409, 649, 948
MvaI CCWGG 4 cut(s) 329, 409, 649, 948
MvnI CGCG 3 cut(s) 221, 351, 659
MwoI GCNNNNNNNGC 3 cut(s) 420, 653, 931
NcoI CCATGG 1 cut(s) 742
NlaIII CATG 6 cut(s) 51, 436, 746, 767, 932, 970
NlaIV GGNNCC 5 cut(s) 313, 692, 837, 1106, 1194
NmuCI GTSAC 2 cut(s) 481, 706
NruI TCGCGA 1 cut(s) 221
NspI RCATGY 2 cut(s) 767, 932
PaeR7I CTCGAG 1 cut(s) 383
PceI AGGCCT 1 cut(s) 667
PciI ACATGT 1 cut(s) 928
PciSI GCTCTTC 1 cut(s) 23
PcsI WCGNNNNNNNCGW 5 cut(s) 90, 225, 243, 291, 444
PdmI GAANNNNTTC 1 cut(s) 626
Ple19I CGATCG 2 cut(s) 225, 681
PleI GAGTC 2 cut(s) 52, 722
PpsI GAGTC 2 cut(s) 52, 722
Ppu21I YACGTR 1 cut(s) 481
PscI ACATGT 1 cut(s) 928
PshBI ATTAAT 1 cut(s) 996
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 4 cut(s) 327, 407, 647, 946
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 4 cut(s) 327, 407, 647, 946
PspN4I GGNNCC 5 cut(s) 313, 692, 837, 1106, 1194
PspPI GGNCC 6 cut(s) 13, 208, 312, 645, 674, 1193
PsuI RGATCY 1 cut(s) 1104
PvuI CGATCG 2 cut(s) 225, 681
RruI TCGCGA 1 cut(s) 221
RsaI GTAC 3 cut(s) 479, 527, 1127
RsaNI GTAC 3 cut(s) 478, 526, 1126
SacI GAGCTC 1 cut(s) 35
SacII CCGCGG 1 cut(s) 352
SalI GTCGAC 2 cut(s) 83, 95
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 1 cut(s) 996
Sau96I GGNCC 6 cut(s) 13, 208, 312, 645, 674, 1193
SchI GAGTC 2 cut(s) 53, 722
ScrFI CCNGG 4 cut(s) 329, 409, 649, 948
SduI GDGCHC 3 cut(s) 35, 345, 520
Sfr274I CTCGAG 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 352
SgrBI CCGCGG 1 cut(s) 352
SinI GGWCC 5 cut(s) 13, 208, 312, 674, 1193
SlaI CTCGAG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 5 cut(s) 154, 511, 893, 1090, 1117
SseBI AGGCCT 1 cut(s) 667
SstI GAGCTC 1 cut(s) 35
StuI AGGCCT 1 cut(s) 667
StyD4I CCNGG 4 cut(s) 327, 407, 647, 946
StyI CCWWGG 2 cut(s) 742, 1044
TaaI ACNGT 5 cut(s) 106, 212, 322, 712, 987
TaiI ACGT 6 cut(s) 12, 60, 342, 462, 483, 1035
TaqI TCGA 8 cut(s) 84, 96, 246, 285, 345, 384, 1094, 1115
TasI AATT 5 cut(s) 154, 511, 893, 1090, 1117
TauI GCSGC 7 cut(s) 184, 354, 357, 407, 417, 420, 659
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TscAI CASTG 2 cut(s) 115, 927
TseFI GTSAC 2 cut(s) 481, 706
TseI GCWGC 4 cut(s) 184, 420, 595, 934
Tsp45I GTSAC 2 cut(s) 481, 706
TspDTI ATGAA 4 cut(s) 470, 560, 803, 906
TspRI CASTG 2 cut(s) 115, 927
VpaK11BI GGWCC 5 cut(s) 13, 208, 312, 674, 1193
VspI ATTAAT 1 cut(s) 996
XapI RAATTY 3 cut(s) 893, 1090, 1117
XceI RCATGY 2 cut(s) 767, 932
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 84, 96
XmnI GAANNNNTTC 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.