Rh4CG367100

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014204
Physical Location & Seq
Forward (+)
62216089 .. 62219234
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG367100.1

Sequence Viewer

Length: 1227 bp
ATGGCAGACCTGAAAGCGAAGAAGAGCTCAGCATCAGAGTCATGCAAAGACGTTCCATCTCTCCTCACCTCTTTCGTCGACACCTTCGTCGACTTCACGGTCAGTGGACATTTCCTCTTACCCGAAAACCCCAATATTCCACTTGCCCAAAATTACACTCAACAACCTCCGCCGCCGCCGCCCTTGCAAACCTTCTACCCACCGCAGGACCGTCTGATCGCGATCGGCGATGTCCACGGCGACCTCGACAAGACCAAGCAATCACTACGCCTCGCCAATCTGATCGACCAGTCCGACAAATGGGTCGGAGGCTCCGCCACCGTCGTCCAGGTCGGCGACGTGCTCGACCGCGGCGGCGACGAGATCAAAATCCTGTATTTCCTCGAGAAGCTGAGACGGGAAGCCGCCAGATGCGGCGGCACCTTGATCACCATGCACGGCAACCACGAGATTATGAACGTGGAGGGAGATTTCAGGTACGTGACCAGGGAGGGTTTGGATGAGTTTCTGAATTGGGCTGATTGGTACCGAGTTGGGAACAGAATGAAAGCCCTCTGTAAAGGGGGGTTGGAAAACCCTAAAGACCCATTTGAGGGGCTGCCTTTGGGGGGTTTCCAAAATGTCAAGAAAGAGTTCACGCAAGGTTTTAGGGCCAGGATCGCCGCGCTGAGGCCTGATGGTCCCATCACCGCTCGGTTCTTGTCCAAGAATGTCACTGTGTTGGTTGTGGGGGACTCGGTTTTCGTGCACGGCGGGCTTTTGGCCAAGCATGTTTCGTATGGTCTGGAGAAGATGAATGAGGAGGTGAGGGAGTGGGTGAATGGGGTGAAGGGGAGATTGGCACCCGAGTATTGCCGTGGGGGCAACTCTGTGGTTTGGTTGAGGAAGTTTTCTGATGAATTTGCCGACAGGTGTGATTGTTCGGCACTGCAACATGTGCTGGAGACTATTCCTGGTGCTAAGAGGATGATCATGGGCCATACCATTCAACAGTTTGGGATTAACGGCGCTTGCGACGAGAGAGCTATTCGGATTGACGTGGGCATGTCCAAGGGCTGTGGTGATGGCTTGCCTGAAGTGTTGGAGATCAATGGGAATTCGGGTTTGAGGATCCTGACGTCGAATCTGTTGTACCAGAACAAGTACAAATCTCCTGCCCAAGCTGGGAGTAGGGAAGGGGTTGGATTTTTGGAGCAGGGACCAAGACAAGTGCAAGTGAAGGCTTGA

Protein Analysis

408

Amino Acids

45.02

Weight (kDa)

7.07

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 71 - 204 1.1e-08 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014204)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18480
fragaria_vesca FvH4_3g25460 FvH4_3g25460 FvH4_3g25460
malus_domestica MD02G1292100.v1.1 MD07G1034500.v1.1
prunus_persica Prupe.2G031200_v2.0.a1
pyrus_communis pycom02g24670
rosa_chinensis RchiOBHm_Chr4g0435781
rosa_laevigata RLG00000006559
rosa_multiflora Rmu_sc0000051.1_g000023 Rmu_sc0004389.1_g000004
rosa_roxburghii Rroxscaffold_5G00376970
rosa_rugosa Rorug04G0289600
rosa_samantha Rh4BG352600 Rh4CG367100
rosa_wichuraiana Rw4G030030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 86, 98, 302
AatII GACGTC 1 cut(s) 1121
Acc65I GGTACC 1 cut(s) 525
AccB1I GGYRCC 3 cut(s) 419, 525, 841
AccBSI CCGCTC 1 cut(s) 692
AccI GTMKAC 2 cut(s) 78, 90
AccII CGCG 3 cut(s) 221, 351, 665
AclWI GGATC 3 cut(s) 665, 1105, 1118
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 2 cut(s) 899, 1096
AcuI CTGAAG 1 cut(s) 1095
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 4 cut(s) 479, 527, 1133, 1145
AfiI CCNNNNNNNGG 7 cut(s) 205, 300, 592, 593, 608, 669, 1164
AflIII ACRYGT 1 cut(s) 934
AgsI TTSAA 1 cut(s) 989
AjiI CACGTC 2 cut(s) 340, 1039
AjnI CCWGG 4 cut(s) 327, 485, 653, 952
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
AluBI AGCT 4 cut(s) 27, 391, 1025, 1163
AluI AGCT 4 cut(s) 27, 391, 1025, 1163
Alw21I GWGCWC 3 cut(s) 29, 345, 750
Alw26I GTCTC 2 cut(s) 388, 938
Alw44I GTGCAC 1 cut(s) 746
AlwI GGATC 3 cut(s) 665, 1105, 1118
Ama87I CYCGRG 2 cut(s) 383, 845
AoxI GGCC 4 cut(s) 651, 671, 762, 976
ApaLI GTGCAC 1 cut(s) 746
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 2 cut(s) 899, 1096
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
Asp700I GAANNNNTTC 1 cut(s) 632
Asp718I GGTACC 1 cut(s) 525
AspLEI GCGC 2 cut(s) 667, 1010
AspS9I GGNCC 5 cut(s) 208, 651, 680, 976, 1199
AsuHPI GGTGA 7 cut(s) 58, 421, 679, 817, 829, 838, 1073
AvaI CYCGRG 2 cut(s) 383, 845
AvaII GGWCC 3 cut(s) 208, 680, 1199
BaeGI GKGCMC 1 cut(s) 750
BalI TGGCCA 1 cut(s) 764
BamHI GGATCC 1 cut(s) 1110
BanI GGYRCC 3 cut(s) 419, 525, 841
BanII GRGCYC 1 cut(s) 29
BauI CACGAG 1 cut(s) 446
Bbv12I GWGCWC 3 cut(s) 29, 345, 750
BbvCI CCTCAGC 1 cut(s) 668
BbvI GCAGC 1 cut(s) 585
BccI CCATC 4 cut(s) 64, 671, 692, 1058
BceAI ACGGC 5 cut(s) 253, 454, 766, 840, 1021
BciT130I CCWGG 4 cut(s) 329, 487, 655, 954
BclI TGATCA 2 cut(s) 426, 969
BcoDI GTCTC 2 cut(s) 388, 938
BfoI RGCGCY 1 cut(s) 1011
BglI GCCNNNNNGGC 1 cut(s) 861
BlpI GCTNAGC 1 cut(s) 28
Bme1390I CCNGG 4 cut(s) 329, 487, 655, 954
Bme18I GGWCC 3 cut(s) 208, 680, 1199
BmeT110I CYCGRG 2 cut(s) 383, 845
BmgBI CACGTC 2 cut(s) 340, 1039
BmgT120I GGNCC 5 cut(s) 208, 651, 680, 976, 1199
BmiI GGNNCC 7 cut(s) 313, 421, 527, 682, 843, 1112, 1200
BmrFI CCNGG 4 cut(s) 329, 487, 655, 954
BmsI GCATC 2 cut(s) 41, 401
BpmI CTGGAG 2 cut(s) 806, 962
Bpu10I CCTNAGC 1 cut(s) 668
Bpu1102I GCTNAGC 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 481
BsaBI GATNNNNATC 2 cut(s) 221, 368
BsaHI GRCGYC 1 cut(s) 1118
BsaJI CCNNGG 5 cut(s) 235, 349, 486, 856, 1050
Bsc4I CCNNNNNNNGG 7 cut(s) 205, 300, 592, 593, 608, 669, 1164
Bse1I ACTGG 1 cut(s) 289
Bse8I GATNNNNATC 2 cut(s) 221, 368
BseBI CCWGG 4 cut(s) 329, 487, 655, 954
BseDI CCNNGG 5 cut(s) 235, 349, 486, 856, 1050
BseGI GGATG 2 cut(s) 505, 972
BseJI GATNNNNATC 2 cut(s) 221, 368
BseLI CCNNNNNNNGG 7 cut(s) 205, 300, 592, 593, 608, 669, 1164
BseMII CTCAG 3 cut(s) 42, 383, 659
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 2 cut(s) 53, 815
BseSI GKGCMC 1 cut(s) 750
BseXI GCAGC 1 cut(s) 585
BseYI CCCAGC 1 cut(s) 1163
Bsh1236I CGCG 3 cut(s) 221, 351, 665
Bsh1285I CGRYCG 2 cut(s) 225, 349
BshFI GGCC 4 cut(s) 653, 673, 764, 978
BshNI GGYRCC 3 cut(s) 419, 525, 841
BsiEI CGRYCG 2 cut(s) 225, 349
BsiHKAI GWGCWC 3 cut(s) 29, 345, 750
BsiHKCI CYCGRG 2 cut(s) 383, 845
BslFI GGGAC 3 cut(s) 666, 746, 1212
BslI CCNNNNNNNGG 7 cut(s) 205, 300, 592, 593, 608, 669, 1164
BsmAI GTCTC 2 cut(s) 388, 938
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 3 cut(s) 666, 746, 1212
BsnI GGCC 4 cut(s) 653, 673, 764, 978
BsoBI CYCGRG 2 cut(s) 383, 845
Bsp1286I GDGCHC 3 cut(s) 29, 345, 750
Bsp143I GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
Bsp1720I GCTNAGC 1 cut(s) 28
Bsp68I TCGCGA 1 cut(s) 221
BspANI GGCC 4 cut(s) 653, 673, 764, 978
BspCNI CTCAG 3 cut(s) 41, 384, 660
BspFNI CGCG 3 cut(s) 221, 351, 665
BspLI GGNNCC 7 cut(s) 313, 421, 527, 682, 843, 1112, 1200
BspPI GGATC 3 cut(s) 665, 1105, 1118
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 3 cut(s) 419, 525, 841
BsrBI CCGCTC 1 cut(s) 692
BsrI ACTGG 1 cut(s) 289
BssECI CCNNGG 5 cut(s) 235, 349, 486, 856, 1050
BssMI GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
BssNI GRCGYC 1 cut(s) 1118
BssSI CACGAG 1 cut(s) 446
BssT1I CCWWGG 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 446
Bst2UI CCWGG 4 cut(s) 329, 487, 655, 954
Bst4CI ACNGT 5 cut(s) 100, 212, 322, 718, 993
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 937
BstBAI YACGTR 1 cut(s) 481
BstC8I GCNNGC 3 cut(s) 755, 1012, 1070
BstDEI CTNAG 4 cut(s) 28, 392, 668, 960
BstDSI CCRYGG 3 cut(s) 235, 349, 856
BstF5I GGATG 2 cut(s) 505, 972
BstFNI CGCG 3 cut(s) 221, 351, 665
BstH2I RGCGCY 1 cut(s) 1011
BstHHI GCGC 2 cut(s) 667, 1010
BstKTI GATC 9 cut(s) 219, 225, 285, 366, 429, 660, 972, 1089, 1113
BstMAI GTCTC 2 cut(s) 388, 938
BstMBI GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
BstMCI CGRYCG 2 cut(s) 225, 349
BstMWI GCNNNNNNNGC 6 cut(s) 178, 184, 659, 754, 861, 937
BstNI CCWGG 4 cut(s) 329, 487, 655, 954
BstNSI RCATGY 3 cut(s) 773, 938, 1048
BstSCI CCNGG 4 cut(s) 327, 485, 653, 952
BstSLI GKGCMC 1 cut(s) 750
BstUI CGCG 3 cut(s) 221, 351, 665
BstV1I GCAGC 1 cut(s) 585
BstX2I RGATCY 1 cut(s) 1110
BstYI RGATCY 1 cut(s) 1110
BsuRI GGCC 4 cut(s) 653, 673, 764, 978
BtgI CCRYGG 3 cut(s) 235, 349, 856
BtgZI GCGATG 1 cut(s) 243
BtrI CACGTC 2 cut(s) 340, 1039
BtsCI GGATG 2 cut(s) 505, 972
BtsI GCAGTG 1 cut(s) 926
BtsIMutI CAGTG 3 cut(s) 109, 714, 926
BtuMI TCGCGA 1 cut(s) 221
Cac8I GCNNGC 3 cut(s) 755, 1012, 1070
CfoI GCGC 2 cut(s) 667, 1010
Cfr13I GGNCC 5 cut(s) 208, 651, 680, 976, 1199
Cfr42I CCGCGG 1 cut(s) 352
Csp6I GTAC 4 cut(s) 478, 526, 1132, 1144
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 6 cut(s) 42, 433, 770, 935, 973, 1045
CviQI GTAC 4 cut(s) 478, 526, 1132, 1144
DdeI CTNAG 4 cut(s) 28, 392, 668, 960
DpnI GATC 9 cut(s) 218, 224, 284, 365, 428, 659, 971, 1088, 1112
DpnII GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
DrdI GACNNNNNNGTC 3 cut(s) 86, 98, 302
DseDI GACNNNNNNGTC 3 cut(s) 86, 98, 302
EaeI YGGCCR 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 2 cut(s) 159, 304
Ecl136II GAGCTC 1 cut(s) 27
Eco130I CCWWGG 1 cut(s) 1050
Eco147I AGGCCT 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 29
Eco47I GGWCC 3 cut(s) 208, 680, 1199
Eco53kI GAGCTC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 1095
Eco88I CYCGRG 2 cut(s) 383, 845
EcoICRI GAGCTC 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 1096
EcoRII CCWGG 4 cut(s) 327, 485, 653, 952
EcoT14I CCWWGG 1 cut(s) 1050
EcoT38I GRGCYC 1 cut(s) 29
ErhI CCWWGG 1 cut(s) 1050
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 6 cut(s) 45, 436, 773, 938, 976, 1048
FaiI YATR 9 cut(s) 43, 434, 455, 771, 780, 936, 974, 981, 1046
FaqI GGGAC 3 cut(s) 666, 746, 1212
FatI CATG 6 cut(s) 41, 432, 769, 934, 972, 1044
FauI CCCGC 1 cut(s) 746
FbaI TGATCA 2 cut(s) 426, 969
FblI GTMKAC 2 cut(s) 78, 90
FokI GGATG 2 cut(s) 512, 979
FriOI GRGCYC 1 cut(s) 29
GlaI GCGC 2 cut(s) 666, 1009
GsaI CCCAGC 1 cut(s) 1167
GsuI CTGGAG 2 cut(s) 806, 962
HaeII RGCGCY 1 cut(s) 1011
HaeIII GGCC 4 cut(s) 653, 673, 764, 978
HhaI GCGC 2 cut(s) 667, 1010
Hin1I GRCGYC 1 cut(s) 1118
Hin1II CATG 6 cut(s) 45, 436, 773, 938, 976, 1048
Hin6I GCGC 2 cut(s) 665, 1008
HinP1I GCGC 2 cut(s) 665, 1008
HincII GTYRAC 2 cut(s) 79, 91
HindII GTYRAC 2 cut(s) 79, 91
HinfI GANTC 3 cut(s) 38, 734, 1123
HphI GGTGA 7 cut(s) 58, 421, 679, 817, 829, 838, 1073
Hpy166II GTNNAC 6 cut(s) 79, 91, 107, 235, 636, 748
Hpy188I TCNGA 8 cut(s) 37, 216, 282, 295, 308, 510, 895, 1032
Hpy188III TCNNGA 5 cut(s) 220, 385, 625, 785, 1114
Hpy8I GTNNAC 6 cut(s) 79, 91, 107, 235, 636, 748
Hpy99I CGWCG 7 cut(s) 80, 92, 326, 341, 362, 1019, 1123
HpyAV CCTTC 5 cut(s) 94, 202, 823, 1169, 1213
HpyCH4III ACNGT 5 cut(s) 100, 212, 322, 718, 993
HpyCH4IV ACGT 6 cut(s) 51, 339, 459, 480, 1038, 1118
HpyCH4V TGCA 6 cut(s) 45, 187, 436, 748, 931, 1213
HpyF10VI GCNNNNNNNGC 6 cut(s) 178, 184, 659, 754, 861, 937
HpyF3I CTNAG 4 cut(s) 28, 392, 668, 960
HpySE526I ACGT 6 cut(s) 51, 339, 459, 480, 1038, 1118
Hsp92I GRCGYC 1 cut(s) 1118
Hsp92II CATG 6 cut(s) 45, 436, 773, 938, 976, 1048
HspAI GCGC 2 cut(s) 665, 1008
KpnI GGTACC 1 cut(s) 529
Ksp22I TGATCA 2 cut(s) 426, 969
KspI CCGCGG 1 cut(s) 352
Kzo9I GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 2 cut(s) 317, 1192
Lsp1109I GCAGC 1 cut(s) 585
LweI GCATC 2 cut(s) 41, 401
MaeII ACGT 6 cut(s) 51, 339, 459, 480, 1038, 1118
MaeIII GTNAC 2 cut(s) 481, 712
MalI GATC 9 cut(s) 218, 224, 284, 365, 428, 659, 971, 1088, 1112
MbiI CCGCTC 1 cut(s) 692
MboI GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
MboII GAAGA 3 cut(s) 31, 34, 802
MflI RGATCY 1 cut(s) 1110
MhlI GDGCHC 3 cut(s) 29, 345, 750
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 4 cut(s) 151, 511, 899, 1096
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 2 cut(s) 47, 728
MmeI TCCRAC 5 cut(s) 286, 318, 549, 1062, 1162
Mox20I TGGCCA 1 cut(s) 764
MroXI GAANNNNTTC 1 cut(s) 632
MscI TGGCCA 1 cut(s) 764
MseI TTAA 1 cut(s) 1002
Msp20I TGGCCA 1 cut(s) 764
MspA1I CMGCKG 1 cut(s) 351
MspR9I CCNGG 4 cut(s) 329, 487, 655, 954
MvaI CCWGG 4 cut(s) 329, 487, 655, 954
MvnI CGCG 3 cut(s) 221, 351, 665
MwoI GCNNNNNNNGC 6 cut(s) 178, 184, 659, 754, 861, 937
NdeII GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
NlaIII CATG 6 cut(s) 45, 436, 773, 938, 976, 1048
NlaIV GGNNCC 7 cut(s) 313, 421, 527, 682, 843, 1112, 1200
NmuCI GTSAC 2 cut(s) 481, 712
NruI TCGCGA 1 cut(s) 221
NspI RCATGY 3 cut(s) 773, 938, 1048
PaeR7I CTCGAG 1 cut(s) 383
PceI AGGCCT 1 cut(s) 673
PciI ACATGT 1 cut(s) 934
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 6 cut(s) 84, 225, 243, 291, 444, 1011
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 1123
Ple19I CGATCG 1 cut(s) 225
PleI GAGTC 2 cut(s) 46, 728
PpsI GAGTC 2 cut(s) 46, 728
Ppu21I YACGTR 1 cut(s) 481
PscI ACATGT 1 cut(s) 934
Psp124BI GAGCTC 1 cut(s) 29
Psp6I CCWGG 4 cut(s) 327, 485, 653, 952
PspFI CCCAGC 1 cut(s) 1163
PspGI CCWGG 4 cut(s) 327, 485, 653, 952
PspN4I GGNNCC 7 cut(s) 313, 421, 527, 682, 843, 1112, 1200
PspPI GGNCC 5 cut(s) 208, 651, 680, 976, 1199
PsuI RGATCY 1 cut(s) 1110
PvuI CGATCG 1 cut(s) 225
RruI TCGCGA 1 cut(s) 221
RsaI GTAC 4 cut(s) 479, 527, 1133, 1145
RsaNI GTAC 4 cut(s) 478, 526, 1132, 1144
SacI GAGCTC 1 cut(s) 29
SacII CCGCGG 1 cut(s) 352
SalI GTCGAC 2 cut(s) 77, 89
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 1002
Sau3AI GATC 9 cut(s) 216, 222, 282, 363, 426, 657, 969, 1086, 1110
Sau96I GGNCC 5 cut(s) 208, 651, 680, 976, 1199
SchI GAGTC 2 cut(s) 47, 728
ScrFI CCNGG 4 cut(s) 329, 487, 655, 954
SduI GDGCHC 3 cut(s) 29, 345, 750
SfaNI GCATC 2 cut(s) 41, 401
Sfr274I CTCGAG 1 cut(s) 383
Sfr303I CCGCGG 1 cut(s) 352
SgrBI CCGCGG 1 cut(s) 352
SinI GGWCC 3 cut(s) 208, 680, 1199
SlaI CTCGAG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 4 cut(s) 151, 511, 899, 1096
SseBI AGGCCT 1 cut(s) 673
SspI AATATT 1 cut(s) 136
SstI GAGCTC 1 cut(s) 29
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 4 cut(s) 327, 485, 653, 952
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 5 cut(s) 100, 212, 322, 718, 993
TaiI ACGT 6 cut(s) 54, 342, 462, 483, 1041, 1121
TaqI TCGA 7 cut(s) 78, 90, 246, 285, 345, 384, 1121
TasI AATT 4 cut(s) 151, 511, 899, 1096
TatI WGTACW 1 cut(s) 1143
TauI GCSGC 9 cut(s) 175, 178, 181, 354, 357, 407, 417, 420, 665
TfiI GAWTC 1 cut(s) 1123
Tru1I TTAA 1 cut(s) 1002
Tru9I TTAA 1 cut(s) 1002
TscAI CASTG 3 cut(s) 109, 721, 933
TseFI GTSAC 2 cut(s) 481, 712
TseI GCWGC 1 cut(s) 598
Tsp45I GTSAC 2 cut(s) 481, 712
TspDTI ATGAA 4 cut(s) 470, 560, 809, 912
TspRI CASTG 3 cut(s) 109, 721, 933
VneI GTGCAC 1 cut(s) 746
VpaK11BI GGWCC 3 cut(s) 208, 680, 1199
XapI RAATTY 2 cut(s) 899, 1096
XceI RCATGY 3 cut(s) 773, 938, 1048
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 78, 90
XmnI GAANNNNTTC 1 cut(s) 632
ZraI GACGTC 1 cut(s) 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.