Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0015577

Physical Location & Seq

Genomic Coordinates Forward (+)

18394863 .. 18397116

Gene Structure 2254 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g25490.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGATGGACACCCTCGTCAGATTGTTTCCACCACCACCACCACTGCTACTACCAACCAAGATCAGTCAACTCTTCCATGCATCCAGCCCTCCATGCTCTTTCCCCAAACCCCATTACACAGGAAAGAGATTTTCCAGAGTCCAAAGCAGCAAGTATGGGAATTTCCTGGACTTGAAACCAGAGTCAACACCTGAAGTGTTGGATTTTGATCTGCCCCGGTTGGACCCGAGTAGCCGGTCTCGAGTGGATGTGATCATCATCGGCACAGGGCCTGCTGGTCTTCGTTTAGCTGAGCAAGTGTCACAGTATGGCATCAAGGTATGTTGTGTTGATCCTTCACCTCTTTCTATGTGGCCAAATAACTATGGTGTATGGGTTGATGAGTTTGAGAGCTTGGAGCTTGAAAGTTGTTTAGACAAAACATGGCCTATGGCTTGTGTTCATATTGATGATAATAAGACCAAGTATTTAGACCGTCCTTATGGTCGGGTCAGTAGAAAGAAGCTCAAGAAATTGTTGATGGAGAGGTGTCTCTCCAATGGAGTCAAATTTCACAAGGCCAAGGTTTGGAAAATTGAGCACAAAGAGTTTGAGTCTTTGATTGTGTGTGATGATGGGAATGAGTTGAAGGCAAGCTTAGTTGTTGATGCTAGTGGTTTTGCAAGTAGTTTCATAGAGTATGATAAGCCAAGGAATCATGGGTATCAGATTGCTCATGGTATTTTGGCTGAAGTGGAAGGTCACCCCTTTGACTTGGATAAGATGGTTCTTATGGATTGGAGGGATTCTCATCTAGGGAATGAACCTTACTTGCGTACTGGTAATTCTAGGTTCCCTGCTTTTCTGTATGCAATGCCATTTGATTCCAACTTGGTATTTTTGGAAGAGACTTCACTGGTTAGTAGGCCGGTTTTATCTTACATGGAGATCAAGAAAAGAATGGTGGCAAGGCTAAGGCATTTGGGGATTCGAGTGAAGAGGATATTGGAGGTTGAGAAGTGTGTGGTTCCAATGGGAGGCCCGCTCCCTCGGATACCTCAAAGCGTTATGGCAATTGGAGGGACTTCTGGGATTGTCCACCCTTCAACTGGTTACATGGTGGCTCGGACAATGGCTCTAGCACCGGTACTAGCTGAGGCCATTGCAGAGTGCCTTGGTTCAACTCGAATGATCCGAGGGAAATCGCTTCATGAACGAGCGTGGAATGGTTTGTGGACAACTGAGAGGAGATGTGTAAGGCAATATTACTCTTTTGGGATGGAAACTTTGTTGAAGCTTGATCTTAATGGAAGTAGAAGGTTTTTTGATGCTTTCTTTGACTTGGATCCTTATTACTGGCAAGGATTCCTCTCCTCAAGGTTGTCTCTTAGAGAGCTTGCCTTGTTGAGTTTATCTCTGTTTGGCCATGCCTCATTTCCGTCTAGGTTCGATATTGTTTCCAAGTGTCCTGTGCCCTTTGTTAAAATGATCAGTAATCTAGCACTAGAAACCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009687 GO:0009688 GO:0009987 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016853 GO:0016860 GO:0019752 GO:0032787 GO:0034020 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046165 GO:0046394 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

Lycopene_cycl Trp_halogenase

Protein Analysis

499

Amino Acids

56.51

Weight (kDa)

8.89

Isoelectric Point (pI)

48.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lycopene_cycl	PF05834	83 - 475	1.5e-146	Lycopene cyclase protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015577)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g25490
malus_domestica	MD03G1148700.v1.1 MD11G1168000.v1.1
prunus_persica	Prupe.6G135000_v2.0.a1
pyrus_communis	pycom03g10310 pycom11g14030
rosa_chinensis	RchiOBHm_Chr5g0046551
rosa_laevigata	RLG00000034417
rosa_multiflora	Rmu_sc0003878.1_g000038
rosa_roxburghii	Rroxscaffold_1G00034850
rosa_rugosa	Rorug05G0229500
rosa_samantha	Rh5AG309800 Rh5BG318200 Rh5CG343900 Rh5DG328500
rosa_wichuraiana	Rw5G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	14
AccB7I	CCANNNNNTGG	1 cut(s)	567
AccBSI	CCGCTC	1 cut(s)	1024
AciI	CCGC	1 cut(s)	1022
AclWI	GGATC	4 cut(s)	326, 1165, 1319, 1332
AcoI	YGGCCR	2 cut(s)	353, 1402
AcsI	RAATTY	2 cut(s)	160, 548
AcuI	CTGAAG	2 cut(s)	213, 750
AfaI	GTAC	2 cut(s)	817, 1128
AfiI	CCNNNNNNNGG	3 cut(s)	567, 1016, 1088
AgeI	ACCGGT	1 cut(s)	1123
AgsI	TTSAA	6 cut(s)	175, 404, 628, 1086, 1161, 1273
AjnI	CCWGG	1 cut(s)	165
AjuI	GAANNNNNNNTTGG	2 cut(s)	968, 1000
AluBI	AGCT	8 cut(s)	290, 393, 400, 505, 636, 1133, 1276, 1375
AluI	AGCT	8 cut(s)	290, 393, 400, 505, 636, 1133, 1276, 1375
Alw21I	GWGCWC	1 cut(s)	582
Alw26I	GTCTC	4 cut(s)	243, 536, 881, 1368
AlwI	GGATC	4 cut(s)	326, 1165, 1319, 1332
AlwNI	CAGNNNCTG	1 cut(s)	272
Ama87I	CYCGRG	2 cut(s)	226, 240
AoxI	GGCC	8 cut(s)	269, 353, 425, 558, 905, 1018, 1137, 1402
ApeKI	GCWGC	1 cut(s)	147
ApoI	RAATTY	2 cut(s)	160, 548
AsiGI	ACCGGT	1 cut(s)	1123
AspS9I	GGNCC	3 cut(s)	223, 269, 1019
AsuC2I	CCSGG	1 cut(s)	217
AsuHPI	GGTGA	2 cut(s)	330, 734
AvaI	CYCGRG	2 cut(s)	226, 240
AvaII	GGWCC	1 cut(s)	223
BaeGI	GKGCMC	1 cut(s)	1455
BalI	TGGCCA	2 cut(s)	355, 1404
BamHI	GGATCC	1 cut(s)	1324
BbsI	GAAGAC	1 cut(s)	272
Bbv12I	GWGCWC	1 cut(s)	582
BbvCI	CCTCAGC	1 cut(s)	1134
BbvI	GCAGC	1 cut(s)	159
BccI	CCATC	4 cut(s)	514, 608, 757, 1252
BciT130I	CCWGG	1 cut(s)	167
BciVI	GTATCC	1 cut(s)	1026
BclI	TGATCA	2 cut(s)	252, 1467
BcnI	CCSGG	1 cut(s)	217
BcoDI	GTCTC	4 cut(s)	243, 536, 881, 1368
BfaI	CTAG	8 cut(s)	651, 794, 828, 1118, 1130, 1422, 1478, 1484
BfuI	GTATCC	1 cut(s)	1026
BisI	GCNGC	1 cut(s)	148
BlpI	GCTNAGC	1 cut(s)	291
BlsI	GCNGC	1 cut(s)	149
Bme1390I	CCNGG	2 cut(s)	167, 217
Bme18I	GGWCC	1 cut(s)	223
BmeT110I	CYCGRG	2 cut(s)	226, 240
BmgT120I	GGNCC	3 cut(s)	223, 269, 1019
BmiI	GGNNCC	4 cut(s)	225, 833, 1008, 1326
BmrFI	CCNGG	2 cut(s)	167, 217
BmsI	GCATC	4 cut(s)	89, 321, 637, 1297
BpiI	GAAGAC	1 cut(s)	272
BplI	GAGNNNNNCTC	6 cut(s)	772, 804, 1008, 1040, 1378, 1410
Bpu10I	CCTNAGC	2 cut(s)	953, 1134
Bpu1102I	GCTNAGC	1 cut(s)	291
BpuEI	CTTGAG	2 cut(s)	491, 1339
BpuMI	CCSGG	1 cut(s)	217
BsaBI	GATNNNNATC	3 cut(s)	207, 257, 789
BsaI	GGTCTC	1 cut(s)	243
BsaJI	CCNNGG	6 cut(s)	215, 561, 689, 1028, 1153, 1174
BsaWI	WCCGGW	1 cut(s)	1123
Bsc4I	CCNNNNNNNGG	3 cut(s)	567, 1016, 1088
Bse118I	RCCGGY	3 cut(s)	234, 907, 1123
Bse1I	ACTGG	4 cut(s)	823, 900, 1093, 1340
Bse3DI	GCAATG	2 cut(s)	858, 1140
Bse8I	GATNNNNATC	3 cut(s)	207, 257, 789
BseBI	CCWGG	1 cut(s)	167
BseDI	CCNNGG	6 cut(s)	215, 561, 689, 1028, 1153, 1174
BseGI	GGATG	3 cut(s)	80, 253, 1263
BseJI	GATNNNNATC	3 cut(s)	207, 257, 789
BseLI	CCNNNNNNNGG	3 cut(s)	567, 1016, 1088
BseMI	GCAATG	2 cut(s)	858, 1140
BseMII	CTCAG	3 cut(s)	282, 1125, 1212
BseNI	ACTGG	4 cut(s)	823, 900, 1093, 1340
BseRI	GAGGAG	2 cut(s)	1240, 1342
BseSI	GKGCMC	1 cut(s)	1455
BseXI	GCAGC	1 cut(s)	159
BshFI	GGCC	8 cut(s)	271, 355, 427, 560, 907, 1020, 1139, 1404
BshTI	ACCGGT	1 cut(s)	1123
BsiHKAI	GWGCWC	1 cut(s)	582
BsiHKCI	CYCGRG	2 cut(s)	226, 240
BsiSI	CCGG	4 cut(s)	217, 235, 908, 1124
BslFI	GGGAC	1 cut(s)	1075
BslI	CCNNNNNNNGG	3 cut(s)	567, 1016, 1088
BsmAI	GTCTC	4 cut(s)	243, 536, 881, 1368
BsmFI	GGGAC	1 cut(s)	1075
BsnI	GGCC	8 cut(s)	271, 355, 427, 560, 907, 1020, 1139, 1404
Bso31I	GGTCTC	1 cut(s)	243
BsoBI	CYCGRG	2 cut(s)	226, 240
Bsp1286I	GDGCHC	2 cut(s)	582, 1455
Bsp143I	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
Bsp1720I	GCTNAGC	1 cut(s)	291
BspACI	CCGC	1 cut(s)	1022
BspANI	GGCC	8 cut(s)	271, 355, 427, 560, 907, 1020, 1139, 1404
BspCNI	CTCAG	3 cut(s)	283, 1126, 1213
BspHI	TCATGA	1 cut(s)	1189
BspLI	GGNNCC	4 cut(s)	225, 833, 1008, 1326
BspPI	GGATC	4 cut(s)	326, 1165, 1319, 1332
BspTNI	GGTCTC	1 cut(s)	243
BsrBI	CCGCTC	1 cut(s)	1024
BsrDI	GCAATG	2 cut(s)	858, 1140
BsrFI	RCCGGY	3 cut(s)	234, 907, 1123
BsrI	ACTGG	4 cut(s)	823, 900, 1093, 1340
BssAI	RCCGGY	3 cut(s)	234, 907, 1123
BssECI	CCNNGG	6 cut(s)	215, 561, 689, 1028, 1153, 1174
BssMI	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
BssT1I	CCWWGG	3 cut(s)	561, 689, 1153
Bst2UI	CCWGG	1 cut(s)	167
Bst4CI	ACNGT	2 cut(s)	306, 476
Bst6I	CTCTTC	3 cut(s)	77, 879, 971
BstC8I	GCNNGC	4 cut(s)	273, 634, 1022, 1377
BstDEI	CTNAG	6 cut(s)	291, 637, 953, 1134, 1221, 1367
BstEII	GGTNACC	1 cut(s)	740
BstF5I	GGATG	3 cut(s)	80, 253, 1263
BstKTI	GATC	9 cut(s)	63, 211, 255, 334, 930, 1173, 1282, 1327, 1470
BstMAI	GTCTC	4 cut(s)	243, 536, 881, 1368
BstMBI	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
BstMWI	GCNNNNNNNGC	1 cut(s)	93
BstNI	CCWGG	1 cut(s)	167
BstPI	GGTNACC	1 cut(s)	740
BstSCI	CCNGG	2 cut(s)	165, 215
BstSLI	GKGCMC	1 cut(s)	1455
BstV1I	GCAGC	1 cut(s)	159
BstV2I	GAAGAC	1 cut(s)	272
BstX2I	RGATCY	1 cut(s)	1324
BstYI	RGATCY	1 cut(s)	1324
BsuI	GTATCC	1 cut(s)	1026
BsuRI	GGCC	8 cut(s)	271, 355, 427, 560, 907, 1020, 1139, 1404
BtsCI	GGATG	3 cut(s)	80, 253, 1263
BtsI	GCAGTG	1 cut(s)	41
BtsIMutI	CAGTG	2 cut(s)	41, 893
Cac8I	GCNNGC	4 cut(s)	273, 634, 1022, 1377
CaiI	CAGNNNCTG	1 cut(s)	272
CciI	TCATGA	1 cut(s)	1189
Cfr10I	RCCGGY	3 cut(s)	234, 907, 1123
Cfr13I	GGNCC	3 cut(s)	223, 269, 1019
Csp6I	GTAC	2 cut(s)	816, 1127
CspAI	ACCGGT	1 cut(s)	1123
CviQI	GTAC	2 cut(s)	816, 1127
DdeI	CTNAG	6 cut(s)	291, 637, 953, 1134, 1221, 1367
DpnI	GATC	9 cut(s)	62, 210, 254, 333, 929, 1172, 1281, 1326, 1469
DpnII	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
DrdI	GACNNNNNNGTC	1 cut(s)	14
DseDI	GACNNNNNNGTC	1 cut(s)	14
EaeI	YGGCCR	2 cut(s)	353, 1402
Eam1104I	CTCTTC	3 cut(s)	77, 879, 971
EarI	CTCTTC	3 cut(s)	77, 879, 971
Eco130I	CCWWGG	3 cut(s)	561, 689, 1153
Eco31I	GGTCTC	1 cut(s)	243
Eco47I	GGWCC	1 cut(s)	223
Eco57I	CTGAAG	2 cut(s)	213, 750
Eco88I	CYCGRG	2 cut(s)	226, 240
Eco91I	GGTNACC	1 cut(s)	740
EcoO109I	RGGNCCY	1 cut(s)	269
EcoO65I	GGTNACC	1 cut(s)	740
EcoRII	CCWGG	1 cut(s)	165
EcoT14I	CCWWGG	3 cut(s)	561, 689, 1153
EcoT22I	ATGCAT	1 cut(s)	82
ErhI	CCWWGG	3 cut(s)	561, 689, 1153
FalI	AAGNNNNNCTT	2 cut(s)	620, 652
FaqI	GGGAC	1 cut(s)	1075
FauI	CCCGC	1 cut(s)	1029
FbaI	TGATCA	2 cut(s)	252, 1467
Fnu4HI	GCNGC	1 cut(s)	148
FokI	GGATG	3 cut(s)	67, 260, 1270
Fsp4HI	GCNGC	1 cut(s)	148
FspBI	CTAG	8 cut(s)	651, 794, 828, 1118, 1130, 1422, 1478, 1484
GluI	GCNGC	1 cut(s)	148
HaeIII	GGCC	8 cut(s)	271, 355, 427, 560, 907, 1020, 1139, 1404
HapII	CCGG	4 cut(s)	217, 235, 908, 1124
HincII	GTYRAC	2 cut(s)	68, 186
HindII	GTYRAC	2 cut(s)	68, 186
HindIII	AAGCTT	2 cut(s)	634, 1274
HinfI	GANTC	9 cut(s)	138, 182, 543, 593, 694, 785, 863, 967, 1344
HpaII	CCGG	4 cut(s)	217, 235, 908, 1124
HphI	GGTGA	2 cut(s)	330, 734
Hpy166II	GTNNAC	4 cut(s)	68, 186, 1078, 1215
Hpy188I	TCNGA	5 cut(s)	20, 708, 1032, 1107, 1175
Hpy188III	TCNNGA	5 cut(s)	135, 240, 508, 931, 1190
Hpy8I	GTNNAC	4 cut(s)	68, 186, 1078, 1215
HpyAV	CCTTC	5 cut(s)	345, 622, 731, 1092, 1290
HpyCH4III	ACNGT	2 cut(s)	306, 476
HpyCH4V	TGCA	4 cut(s)	80, 662, 851, 1145
HpyF10VI	GCNNNNNNNGC	1 cut(s)	93
HpyF3I	CTNAG	6 cut(s)	291, 637, 953, 1134, 1221, 1367
Ksp22I	TGATCA	2 cut(s)	252, 1467
Kzo9I	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
LmnI	GCTCC	2 cut(s)	397, 1029
Lsp1109I	GCAGC	1 cut(s)	159
LweI	GCATC	4 cut(s)	89, 321, 637, 1297
MaeI	CTAG	8 cut(s)	651, 794, 828, 1118, 1130, 1422, 1478, 1484
MaeIII	GTNAC	3 cut(s)	300, 740, 1091
MalI	GATC	9 cut(s)	62, 210, 254, 333, 929, 1172, 1281, 1326, 1469
MbiI	CCGCTC	1 cut(s)	1024
MboI	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
MboII	GAAGA	4 cut(s)	64, 272, 896, 988
MfeI	CAATTG	1 cut(s)	1053
MflI	RGATCY	1 cut(s)	1324
MhlI	GDGCHC	2 cut(s)	582, 1455
MlsI	TGGCCA	2 cut(s)	355, 1404
MluCI	AATT	6 cut(s)	160, 512, 548, 573, 823, 1053
MluNI	TGGCCA	2 cut(s)	355, 1404
MlyI	GAGTC	4 cut(s)	147, 191, 552, 602
MmeI	TCCRAC	3 cut(s)	180, 201, 891
Mox20I	TGGCCA	2 cut(s)	355, 1404
Mph1103I	ATGCAT	1 cut(s)	82
MscI	TGGCCA	2 cut(s)	355, 1404
MseI	TTAA	2 cut(s)	1284, 1461
MslI	CAYNNNNRTG	1 cut(s)	447
Msp20I	TGGCCA	2 cut(s)	355, 1404
MspI	CCGG	4 cut(s)	217, 235, 908, 1124
MspR9I	CCNGG	2 cut(s)	167, 217
MunI	CAATTG	1 cut(s)	1053
MvaI	CCWGG	1 cut(s)	167
MwoI	GCNNNNNNNGC	1 cut(s)	93
NciI	CCSGG	1 cut(s)	217
NdeII	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
NlaIV	GGNNCC	4 cut(s)	225, 833, 1008, 1326
NmuCI	GTSAC	2 cut(s)	300, 740
NsiI	ATGCAT	1 cut(s)	82
PaeR7I	CTCGAG	1 cut(s)	240
PagI	TCATGA	1 cut(s)	1189
PcsI	WCGNNNNNNNCGW	1 cut(s)	1171
PfeI	GAWTC	5 cut(s)	694, 785, 863, 967, 1344
PflMI	CCANNNNNTGG	1 cut(s)	567
PfoI	TCCNGGA	1 cut(s)	165
PinAI	ACCGGT	1 cut(s)	1123
PkrI	GCNGC	1 cut(s)	149
PleI	GAGTC	4 cut(s)	146, 190, 551, 601
PpsI	GAGTC	4 cut(s)	146, 190, 551, 601
Psp6I	CCWGG	1 cut(s)	165
PspEI	GGTNACC	1 cut(s)	740
PspGI	CCWGG	1 cut(s)	165
PspN4I	GGNNCC	4 cut(s)	225, 833, 1008, 1326
PspPI	GGNCC	3 cut(s)	223, 269, 1019
PstNI	CAGNNNCTG	1 cut(s)	272
PsuI	RGATCY	1 cut(s)	1324
RsaI	GTAC	2 cut(s)	817, 1128
RsaNI	GTAC	2 cut(s)	816, 1127
RseI	CAYNNNNRTG	1 cut(s)	447
SaqAI	TTAA	2 cut(s)	1284, 1461
SatI	GCNGC	1 cut(s)	148
Sau3AI	GATC	9 cut(s)	60, 208, 252, 331, 927, 1170, 1279, 1324, 1467
Sau96I	GGNCC	3 cut(s)	223, 269, 1019
SchI	GAGTC	4 cut(s)	147, 191, 552, 602
ScrFI	CCNGG	2 cut(s)	167, 217
SduI	GDGCHC	2 cut(s)	582, 1455
SfaNI	GCATC	4 cut(s)	89, 321, 637, 1297
Sfr274I	CTCGAG	1 cut(s)	240
SinI	GGWCC	1 cut(s)	223
SlaI	CTCGAG	1 cut(s)	240
SmiMI	CAYNNNNRTG	1 cut(s)	447
SmlI	CTYRAG	3 cut(s)	240, 506, 1354
SmoI	CTYRAG	3 cut(s)	240, 506, 1354
Sse9I	AATT	6 cut(s)	160, 512, 548, 573, 823, 1053
SsiI	CCGC	1 cut(s)	1022
SspI	AATATT	1 cut(s)	1244
SspMI	CTAG	8 cut(s)	651, 794, 828, 1118, 1130, 1422, 1478, 1484
StyD4I	CCNGG	2 cut(s)	165, 215
StyI	CCWWGG	3 cut(s)	561, 689, 1153
TaaI	ACNGT	2 cut(s)	306, 476
TaqI	TCGA	4 cut(s)	241, 970, 1165, 1428
TasI	AATT	6 cut(s)	160, 512, 548, 573, 823, 1053
TfiI	GAWTC	5 cut(s)	694, 785, 863, 967, 1344
Tru1I	TTAA	2 cut(s)	1284, 1461
Tru9I	TTAA	2 cut(s)	1284, 1461
TscAI	CASTG	2 cut(s)	48, 900
TseFI	GTSAC	2 cut(s)	300, 740
TseI	GCWGC	1 cut(s)	147
Tsp45I	GTSAC	2 cut(s)	300, 740
TspDTI	ATGAA	5 cut(s)	431, 661, 816, 1178, 1206
TspGWI	ACGGA	1 cut(s)	1407
TspRI	CASTG	2 cut(s)	48, 900
Van91I	CCANNNNNTGG	1 cut(s)	567
VpaK11BI	GGWCC	1 cut(s)	223
XapI	RAATTY	2 cut(s)	160, 548
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1085
XhoI	CTCGAG	1 cut(s)	240
XspI	CTAG	8 cut(s)	651, 794, 828, 1118, 1130, 1422, 1478, 1484
Zsp2I	ATGCAT	1 cut(s)	82

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.