Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0015577

Physical Location & Seq

Genomic Coordinates Reverse (-)

42454953 .. 42456437

Gene Structure 1485 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG318200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1485 bp

ATGGACACCTTTGTCAGCTTGTTTTCACCGCCACCACCACTACCAACCAAGACCAGTCAACTCTTTCATGCATCCAGTCCTCCATTCTCTTTGCCAAAAACCCATCACTCAAGAAAGAGATTTTCCAGAGTCCAAAGCAAGTATGGAAATTTTCTGGACTTGAAACCAGAGTCAAAACCTGAAGTGCTAGACTTTGATCTGCCCCGGTTGGACCCTTCTAGCCGGTTTCGGTCAGATGTGATCATCATTGGCACAGGCCCTGCTGGTCTTCGTCTTGCTGAGCAAGTGTCACAGTATGGCATCAAGGTATGTTGTGTTGATCCTTTACCTCTTTCTATGTGGCCAAATAACTATGGTGTTTGGGTTGATGAGTTTGAGAGCTTGGAGCTTGAAAGTTGCTTAGATAAAACATGGCCTATGGCTTGTGTTCATATTGATGATAATAAGACCAAGTATTTGGACCGTCCTTATGGTCGAGTCAGTAGGAAGAAACTTAAGAATTTGTTGATGGAGAGGTGTCTCTCCAATGGAGTCAAATTTCACCAGGCAAAGGTTTGGAAAATTGAGCACAAAGAGTTTGAGTCTTTGATTTTGTGTGATGATGGGAATGAGTTGAAGGCAAGCTTAGTTGTTGATGCCAGTGGTTTTGCAAGCAATTTCGTAGAGTATAATAAGCCAAGGAACCATGGCTATCAGATTGCTCATGGAATTTTGGCAGAAGTGGAGGGTCACCCCTTCGACTTGGACAAGATGCTTCTTATGGATTGGAGGGATTCCCATCTTGGGAATGAACCTTACTTGCGTACTGGTAATTCTAGGATCCCCACTTTTCTGTATGCAATGCCATTTGATTCCAACTTAGTATTCTTGGAAGAGACTTCACTGGTTAGTAGGCCAGTTTTACCTTACATGGAGATTAAGAAAAGAATGGTGGCGAGGCTAAGGCATTTGGGCATTCGAGTGAAGAGGATATTGGAGGTTGAGAAGTGTTTGATTCCAATGGGAGGCCCTCTTCCTCGGATACCTCAAAGCGTTATGGCAATTGGAGGGACTTCTGGGATTGTCCACCCTTCAACTGGTTACATGGTGGCTCGGACAATGGCTCTAGCACCAATACTTGCTGAGGCCATTGCAGAGTGCCTTGGTTCGAGCCGAATGATCAGAGGGAAATCGCTTCATCAACGAGCGTGGAATGGTTTGTGGCCAATTGAGAGGAGATGTGTAAGGGAATATTACTCTTTCGGGATGGAGACTTTGTTGAAGCTTGATCTTAATGGGACTAGAAGGTTTTTTGATGCTTTCTTTGACTTGGATCCTTATTACTGGCAAGGGTTTCTCTCTTCAAGGTTGTCTCTTACAGAGCTTGCCTTGTTGAGTTTATCTCTGTTTGGCCATGCCTCATTTCTGTCTAGGTTCGATATTGTTTCAAAGTGTCCTGCGCCCTTGGTTAAAATGATTGGGAATCTAGCACTAGAAACCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009687 GO:0009688 GO:0009987 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016853 GO:0016860 GO:0019752 GO:0032787 GO:0034020 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046165 GO:0046394 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

Lycopene_cycl Trp_halogenase

Protein Analysis

494

Amino Acids

56.13

Weight (kDa)

8.85

Isoelectric Point (pI)

46.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lycopene_cycl	PF05834	79 - 471	1.2e-146	Lycopene cyclase protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0015577)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g25490
malus_domestica	MD03G1148700.v1.1 MD11G1168000.v1.1
prunus_persica	Prupe.6G135000_v2.0.a1
pyrus_communis	pycom03g10310 pycom11g14030
rosa_chinensis	RchiOBHm_Chr5g0046551
rosa_laevigata	RLG00000034417
rosa_multiflora	Rmu_sc0003878.1_g000038
rosa_roxburghii	Rroxscaffold_1G00034850
rosa_rugosa	Rorug05G0229500
rosa_samantha	Rh5AG309800 Rh5BG318200 Rh5CG343900 Rh5DG328500
rosa_wichuraiana	Rw5G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	11
AciI	CCGC	1 cut(s)	29
AclWI	GGATC	5 cut(s)	314, 814, 827, 1307, 1320
AcoI	YGGCCR	3 cut(s)	341, 1202, 1390
AcsI	RAATTY	4 cut(s)	148, 499, 536, 708
AcuI	CTGAAG	1 cut(s)	201
AfaI	GTAC	1 cut(s)	805
AfiI	CCNNNNNNNGG	4 cut(s)	550, 783, 1004, 1076
AflII	CTTAAG	1 cut(s)	494
AgsI	TTSAA	7 cut(s)	163, 392, 616, 1074, 1261, 1344, 1428
AjnI	CCWGG	1 cut(s)	543
AjuI	GAANNNNNNNTTGG	4 cut(s)	848, 880, 956, 988
AluBI	AGCT	6 cut(s)	18, 381, 388, 624, 1264, 1363
AluI	AGCT	6 cut(s)	18, 381, 388, 624, 1264, 1363
Alw21I	GWGCWC	1 cut(s)	570
Alw26I	GTCTC	4 cut(s)	524, 869, 1244, 1356
AlwI	GGATC	5 cut(s)	314, 814, 827, 1307, 1320
AlwNI	CAGNNNCTG	1 cut(s)	260
AoxI	GGCC	8 cut(s)	256, 341, 413, 893, 1006, 1125, 1202, 1390
ApoI	RAATTY	4 cut(s)	148, 499, 536, 708
ArsI	GACNNNNNNTTYG	2 cut(s)	439, 471
AspLEI	GCGC	1 cut(s)	1441
AspS9I	GGNCC	4 cut(s)	211, 257, 460, 1007
AsuC2I	CCSGG	1 cut(s)	205
AsuHPI	GGTGA	3 cut(s)	18, 533, 722
AvaII	GGWCC	2 cut(s)	211, 460
BalI	TGGCCA	3 cut(s)	343, 1204, 1392
BamHI	GGATCC	2 cut(s)	819, 1312
BbsI	GAAGAC	1 cut(s)	260
Bbv12I	GWGCWC	1 cut(s)	570
BbvCI	CCTCAGC	1 cut(s)	1122
BccI	CCATC	5 cut(s)	111, 502, 596, 786, 1240
BciT130I	CCWGG	1 cut(s)	545
BciVI	GTATCC	1 cut(s)	1014
BclI	TGATCA	2 cut(s)	240, 1158
BcnI	CCSGG	1 cut(s)	205
BcoDI	GTCTC	4 cut(s)	524, 869, 1244, 1356
BfaI	CTAG	8 cut(s)	188, 219, 816, 1106, 1281, 1410, 1466, 1472
BfrI	CTTAAG	1 cut(s)	494
BfuI	GTATCC	1 cut(s)	1014
BlpI	GCTNAGC	1 cut(s)	279
Bme1390I	CCNGG	2 cut(s)	205, 545
Bme18I	GGWCC	2 cut(s)	211, 460
BmgT120I	GGNCC	4 cut(s)	211, 257, 460, 1007
BmiI	GGNNCC	4 cut(s)	213, 683, 821, 1314
BmrFI	CCNGG	2 cut(s)	205, 545
BmsI	GCATC	5 cut(s)	80, 309, 625, 741, 1285
BpiI	GAAGAC	1 cut(s)	260
BplI	GAGNNNNNCTC	2 cut(s)	1366, 1398
Bpu10I	CCTNAGC	2 cut(s)	941, 1122
Bpu1102I	GCTNAGC	1 cut(s)	279
BpuEI	CTTGAG	1 cut(s)	94
BpuMI	CCSGG	1 cut(s)	205
BsaBI	GATNNNNATC	1 cut(s)	777
BsaJI	CCNNGG	6 cut(s)	203, 677, 685, 1016, 1141, 1443
BsaXI	ACNNNNNCTCC	2 cut(s)	1241, 1271
Bsc4I	CCNNNNNNNGG	4 cut(s)	550, 783, 1004, 1076
Bse118I	RCCGGY	1 cut(s)	222
Bse1I	ACTGG	8 cut(s)	54, 75, 639, 811, 888, 896, 1081, 1328
Bse3DI	GCAATG	2 cut(s)	846, 1128
Bse8I	GATNNNNATC	1 cut(s)	777
BseBI	CCWGG	1 cut(s)	545
BseDI	CCNNGG	6 cut(s)	203, 677, 685, 1016, 1141, 1443
BseGI	GGATG	2 cut(s)	71, 1251
BseJI	GATNNNNATC	1 cut(s)	777
BseLI	CCNNNNNNNGG	4 cut(s)	550, 783, 1004, 1076
BseMI	GCAATG	2 cut(s)	846, 1128
BseMII	CTCAG	2 cut(s)	270, 1113
BseNI	ACTGG	8 cut(s)	54, 75, 639, 811, 888, 896, 1081, 1328
BseRI	GAGGAG	1 cut(s)	1228
BshFI	GGCC	8 cut(s)	258, 343, 415, 895, 1008, 1127, 1204, 1392
BsiHKAI	GWGCWC	1 cut(s)	570
BsiSI	CCGG	2 cut(s)	205, 223
BslFI	GGGAC	2 cut(s)	1063, 1291
BslI	CCNNNNNNNGG	4 cut(s)	550, 783, 1004, 1076
BsmAI	GTCTC	4 cut(s)	524, 869, 1244, 1356
BsmFI	GGGAC	2 cut(s)	1063, 1291
BsmI	GAATGC	1 cut(s)	954
BsnI	GGCC	8 cut(s)	258, 343, 415, 895, 1008, 1127, 1204, 1392
Bsp1286I	GDGCHC	1 cut(s)	570
Bsp143I	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
Bsp1720I	GCTNAGC	1 cut(s)	279
Bsp19I	CCATGG	1 cut(s)	685
BspACI	CCGC	1 cut(s)	29
BspANI	GGCC	8 cut(s)	258, 343, 415, 895, 1008, 1127, 1204, 1392
BspCNI	CTCAG	2 cut(s)	271, 1114
BspLI	GGNNCC	4 cut(s)	213, 683, 821, 1314
BspPI	GGATC	5 cut(s)	314, 814, 827, 1307, 1320
BspTI	CTTAAG	1 cut(s)	494
BsrDI	GCAATG	2 cut(s)	846, 1128
BsrFI	RCCGGY	1 cut(s)	222
BsrI	ACTGG	8 cut(s)	54, 75, 639, 811, 888, 896, 1081, 1328
BssAI	RCCGGY	1 cut(s)	222
BssECI	CCNNGG	6 cut(s)	203, 677, 685, 1016, 1141, 1443
BssMI	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
BssT1I	CCWWGG	4 cut(s)	677, 685, 1141, 1443
Bst2UI	CCWGG	1 cut(s)	545
Bst4CI	ACNGT	2 cut(s)	294, 464
Bst6I	CTCTTC	4 cut(s)	867, 959, 1017, 1345
BstAFI	CTTAAG	1 cut(s)	494
BstC8I	GCNNGC	3 cut(s)	622, 652, 1365
BstDEI	CTNAG	6 cut(s)	279, 400, 625, 859, 941, 1122
BstDSI	CCRYGG	1 cut(s)	685
BstEII	GGTNACC	1 cut(s)	728
BstF5I	GGATG	2 cut(s)	71, 1251
BstHHI	GCGC	1 cut(s)	1441
BstKTI	GATC	7 cut(s)	199, 243, 322, 822, 1161, 1270, 1315
BstMAI	GTCTC	4 cut(s)	524, 869, 1244, 1356
BstMBI	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
BstNI	CCWGG	1 cut(s)	545
BstPI	GGTNACC	1 cut(s)	728
BstSCI	CCNGG	2 cut(s)	203, 543
BstV2I	GAAGAC	1 cut(s)	260
BstX2I	RGATCY	2 cut(s)	819, 1312
BstXI	CCANNNNNNTGG	1 cut(s)	457
BstYI	RGATCY	2 cut(s)	819, 1312
BsuI	GTATCC	1 cut(s)	1014
BsuRI	GGCC	8 cut(s)	258, 343, 415, 895, 1008, 1127, 1204, 1392
BtgI	CCRYGG	1 cut(s)	685
BtsCI	GGATG	2 cut(s)	71, 1251
BtsIMutI	CAGTG	2 cut(s)	646, 881
Cac8I	GCNNGC	3 cut(s)	622, 652, 1365
CaiI	CAGNNNCTG	1 cut(s)	260
CfoI	GCGC	1 cut(s)	1441
Cfr10I	RCCGGY	1 cut(s)	222
Cfr13I	GGNCC	4 cut(s)	211, 257, 460, 1007
Csp6I	GTAC	1 cut(s)	804
CspCI	CAANNNNNGTGG	2 cut(s)	1169, 1204
CviAII	CATG	8 cut(s)	68, 411, 686, 704, 910, 1084, 1394, 1480
CviQI	GTAC	1 cut(s)	804
DdeI	CTNAG	6 cut(s)	279, 400, 625, 859, 941, 1122
DpnI	GATC	7 cut(s)	198, 242, 321, 821, 1160, 1269, 1314
DpnII	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
EaeI	YGGCCR	3 cut(s)	341, 1202, 1390
Eam1104I	CTCTTC	4 cut(s)	867, 959, 1017, 1345
EarI	CTCTTC	4 cut(s)	867, 959, 1017, 1345
Eco130I	CCWWGG	4 cut(s)	677, 685, 1141, 1443
Eco47I	GGWCC	2 cut(s)	211, 460
Eco57I	CTGAAG	1 cut(s)	201
Eco91I	GGTNACC	1 cut(s)	728
EcoO109I	RGGNCCY	2 cut(s)	257, 1007
EcoO65I	GGTNACC	1 cut(s)	728
EcoRII	CCWGG	1 cut(s)	543
EcoT14I	CCWWGG	4 cut(s)	677, 685, 1141, 1443
EcoT22I	ATGCAT	1 cut(s)	73
ErhI	CCWWGG	4 cut(s)	677, 685, 1141, 1443
FaeI	CATG	8 cut(s)	71, 414, 689, 707, 913, 1087, 1397, 1483
FalI	AAGNNNNNCTT	2 cut(s)	608, 640
FaqI	GGGAC	2 cut(s)	1063, 1291
FatI	CATG	8 cut(s)	67, 410, 685, 703, 909, 1083, 1393, 1479
FbaI	TGATCA	2 cut(s)	240, 1158
FokI	GGATG	2 cut(s)	58, 1258
FspBI	CTAG	8 cut(s)	188, 219, 816, 1106, 1281, 1410, 1466, 1472
GlaI	GCGC	1 cut(s)	1440
HaeIII	GGCC	8 cut(s)	258, 343, 415, 895, 1008, 1127, 1204, 1392
HapII	CCGG	2 cut(s)	205, 223
HhaI	GCGC	1 cut(s)	1441
Hin1II	CATG	8 cut(s)	71, 414, 689, 707, 913, 1087, 1397, 1483
Hin6I	GCGC	1 cut(s)	1439
HinP1I	GCGC	1 cut(s)	1439
HincII	GTYRAC	1 cut(s)	59
HindII	GTYRAC	1 cut(s)	59
HindIII	AAGCTT	2 cut(s)	622, 1262
HinfI	GANTC	9 cut(s)	129, 170, 477, 531, 581, 773, 851, 994, 1462
HpaII	CCGG	2 cut(s)	205, 223
HphI	GGTGA	3 cut(s)	18, 533, 722
Hpy166II	GTNNAC	2 cut(s)	59, 1066
Hpy188I	TCNGA	5 cut(s)	235, 696, 1020, 1095, 1163
Hpy188III	TCNNGA	4 cut(s)	111, 126, 155, 1243
Hpy8I	GTNNAC	2 cut(s)	59, 1066
HpyAV	CCTTC	5 cut(s)	225, 610, 745, 1080, 1278
HpyCH4III	ACNGT	2 cut(s)	294, 464
HpyCH4V	TGCA	4 cut(s)	71, 650, 839, 1133
HpyF3I	CTNAG	6 cut(s)	279, 400, 625, 859, 941, 1122
Hsp92II	CATG	8 cut(s)	71, 414, 689, 707, 913, 1087, 1397, 1483
HspAI	GCGC	1 cut(s)	1439
Ksp22I	TGATCA	2 cut(s)	240, 1158
Kzo9I	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
LmnI	GCTCC	1 cut(s)	385
LweI	GCATC	5 cut(s)	80, 309, 625, 741, 1285
MaeI	CTAG	8 cut(s)	188, 219, 816, 1106, 1281, 1410, 1466, 1472
MaeIII	GTNAC	3 cut(s)	288, 728, 1079
MalI	GATC	7 cut(s)	198, 242, 321, 821, 1160, 1269, 1314
MboI	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
MboII	GAAGA	6 cut(s)	260, 499, 884, 976, 1004, 1332
MfeI	CAATTG	2 cut(s)	1041, 1206
MflI	RGATCY	2 cut(s)	819, 1312
MhlI	GDGCHC	1 cut(s)	570
MlsI	TGGCCA	3 cut(s)	343, 1204, 1392
MluCI	AATT	9 cut(s)	148, 499, 536, 561, 655, 708, 811, 1041, 1206
MluNI	TGGCCA	3 cut(s)	343, 1204, 1392
MlyI	GAGTC	5 cut(s)	138, 179, 486, 540, 590
MmeI	TCCRAC	2 cut(s)	189, 879
Mox20I	TGGCCA	3 cut(s)	343, 1204, 1392
Mph1103I	ATGCAT	1 cut(s)	73
MscI	TGGCCA	3 cut(s)	343, 1204, 1392
MseI	TTAA	4 cut(s)	495, 918, 1272, 1449
MslI	CAYNNNNRTG	2 cut(s)	435, 959
Msp20I	TGGCCA	3 cut(s)	343, 1204, 1392
MspCI	CTTAAG	1 cut(s)	494
MspI	CCGG	2 cut(s)	205, 223
MspR9I	CCNGG	2 cut(s)	205, 545
MunI	CAATTG	2 cut(s)	1041, 1206
Mva1269I	GAATGC	1 cut(s)	954
MvaI	CCWGG	1 cut(s)	545
NciI	CCSGG	1 cut(s)	205
NcoI	CCATGG	1 cut(s)	685
NdeII	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
NlaIII	CATG	8 cut(s)	71, 414, 689, 707, 913, 1087, 1397, 1483
NlaIV	GGNNCC	4 cut(s)	213, 683, 821, 1314
NmuCI	GTSAC	2 cut(s)	288, 728
NsiI	ATGCAT	1 cut(s)	73
PctI	GAATGC	1 cut(s)	954
PfeI	GAWTC	4 cut(s)	773, 851, 994, 1462
PleI	GAGTC	5 cut(s)	137, 178, 485, 539, 589
PpsI	GAGTC	5 cut(s)	137, 178, 485, 539, 589
Psp6I	CCWGG	1 cut(s)	543
PspEI	GGTNACC	1 cut(s)	728
PspGI	CCWGG	1 cut(s)	543
PspN4I	GGNNCC	4 cut(s)	213, 683, 821, 1314
PspPI	GGNCC	4 cut(s)	211, 257, 460, 1007
PstNI	CAGNNNCTG	1 cut(s)	260
PsuI	RGATCY	2 cut(s)	819, 1312
RsaI	GTAC	1 cut(s)	805
RsaNI	GTAC	1 cut(s)	804
RseI	CAYNNNNRTG	2 cut(s)	435, 959
SaqAI	TTAA	4 cut(s)	495, 918, 1272, 1449
Sau3AI	GATC	7 cut(s)	196, 240, 319, 819, 1158, 1267, 1312
Sau96I	GGNCC	4 cut(s)	211, 257, 460, 1007
SchI	GAGTC	5 cut(s)	138, 179, 486, 540, 590
ScrFI	CCNGG	2 cut(s)	205, 545
SduI	GDGCHC	1 cut(s)	570
SfaNI	GCATC	5 cut(s)	80, 309, 625, 741, 1285
SinI	GGWCC	2 cut(s)	211, 460
SmiMI	CAYNNNNRTG	2 cut(s)	435, 959
SmlI	CTYRAG	2 cut(s)	109, 494
SmoI	CTYRAG	2 cut(s)	109, 494
Sse9I	AATT	9 cut(s)	148, 499, 536, 561, 655, 708, 811, 1041, 1206
SsiI	CCGC	1 cut(s)	29
SspI	AATATT	1 cut(s)	1232
SspMI	CTAG	8 cut(s)	188, 219, 816, 1106, 1281, 1410, 1466, 1472
StyD4I	CCNGG	2 cut(s)	203, 543
StyI	CCWWGG	4 cut(s)	677, 685, 1141, 1443
TaaI	ACNGT	2 cut(s)	294, 464
TaqI	TCGA	5 cut(s)	475, 738, 958, 1148, 1416
TaqII	GACCGA	1 cut(s)	219
TasI	AATT	9 cut(s)	148, 499, 536, 561, 655, 708, 811, 1041, 1206
TfiI	GAWTC	4 cut(s)	773, 851, 994, 1462
Tru1I	TTAA	4 cut(s)	495, 918, 1272, 1449
Tru9I	TTAA	4 cut(s)	495, 918, 1272, 1449
TscAI	CASTG	2 cut(s)	646, 888
TseFI	GTSAC	2 cut(s)	288, 728
Tsp45I	GTSAC	2 cut(s)	288, 728
TspDTI	ATGAA	4 cut(s)	56, 419, 804, 1166
TspRI	CASTG	2 cut(s)	646, 888
Vha464I	CTTAAG	1 cut(s)	494
VpaK11BI	GGWCC	2 cut(s)	211, 460
XapI	RAATTY	4 cut(s)	148, 499, 536, 708
XcmI	CCANNNNNNNNNTGG	2 cut(s)	140, 1073
XspI	CTAG	8 cut(s)	188, 219, 816, 1106, 1281, 1410, 1466, 1472
Zsp2I	ATGCAT	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.