Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0015577

Physical Location & Seq

Genomic Coordinates Reverse (-)

44459716 .. 44461360

Gene Structure 1645 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG309800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1497 bp

ATGGACACCCTTGTCGGATTGTTTTCACCGCCACCGCCACCACCACCACCACTACCAACCAAGACCAGTCAACTCTTTCATGCATCCAGTCCTCCGTTTTCTTTGCCAAAGCCCCATCACTCAAGAAAGAGATTTTCCAGAGTCCAAAGCAAGTATGGAAATTTTCTGGACTTGAAACCAGAGTCAAAACCTGAAGTGTTAGACTTTGATCTGCCCCGGTTGGACCCTTCTAGCCGGTTTCGGTCAGATGTGATCATCATCGGCACAGGCCCTGCTGGTCTTCGTCTTGCTGAGCAAGTGTCACAGTATGGCATCAAGGTATGTTGTGTTGATCCTTCACCTCTTTCTATGTGGCCAAATAACTATGGTGTTTGGGTTGATGAGTTTGAGAGCTTGGAGCTTGAAAGTTGCTTAGACAAAACATGGCCTTTGGCTTGTGTTCATATTGATGATAATAAGACCAAGTATTTGGACCGTCCTTATGGTCGAGTCAGTAGGAAGAAACTTAAGAATTTGTTGATGGAGAGGTGTCTCTCCAATGGAGTCAAATTTCACCAGGCAAAGGTTTGGAAAATTGAGCACAAAGAGTTTGAGTCTTTGATTTTGTGTGATGATGGGAATGAGTTGAAGGCAAGCTTAGTTGTTGATGCCAGTGGTTTTGCAAGCAATTTCGTAGAGTATAATAAGCCAAGGAACCATGGCTATCAGATTGCTCATGGAATTTTGGCTGAAGTGGAGGGTCACCCCTTCGACTTGGACAAGATGCTTCTTATGGATTGGAGGGATTCCCATCTTGGGAATGAACCTTACTTGCGTACTGGTAATTCTAGGATCCCCACTTTTCTGTATGCAATGCCATTTGATTCCAACTTAGTATTCTTGGAAGAGACTTCACTGGTTAGTAGGCCAGTTTTATCTTACATGGAGATTAAGAAAAGAATGGTGGCGAGGCTAAGGCATTTGGGCATTCGAGTGAAGAGGATATTGGAGGTTGAGAAGTGTTTGATTCCAATGGGAGGCCCTCTTCCTCGGATACCTCAAAGCGTTATGGCAATTGGAGGGACTTCTGGGATTGTCCACCCTTCAACTGGTTACATGGTGGCTCGGACAATGGCTCTAGCACCGATACTTGCTGAGGCCATTGCAGAGTGCCTTGGTTCGAGCCGAATGATCAGAGGGAAATCGCTTCATCAACGAGCGTGGAATGGTTTGTGGCCAATTGAGAGGAGATGTGTAAGGGAATATTACTCTTTTGGAATGGAGACTTTGTTGAAGCTTGATCTTAATGGGACTAGAAGGTTTTTTGATGCTTTCTTTGACTTGGATCCTTATTACTGGCAAGGGTTTCTCTCTTCAAGGTTGTCTCTTAGAGAGCTTGCCTTGTTGAGTTTATCTCTGTTTGGCCATGCCTCATTTCTGTCTAGGTTCGATATTGTTTCAAAGTGTCCTGCGCCCTTGGTTAAAATGATTGGGAATCTAGCACTAGAAACCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009687 GO:0009688 GO:0009987 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016853 GO:0016860 GO:0019752 GO:0032787 GO:0034020 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046165 GO:0046394 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

Lycopene_cycl Trp_halogenase

Protein Analysis

498

Amino Acids

56.46

Weight (kDa)

8.93

Isoelectric Point (pI)

47.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lycopene_cycl	PF05834	83 - 475	7.5e-147	Lycopene cyclase protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015577)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g25490
malus_domestica	MD03G1148700.v1.1 MD11G1168000.v1.1
prunus_persica	Prupe.6G135000_v2.0.a1
pyrus_communis	pycom03g10310 pycom11g14030
rosa_chinensis	RchiOBHm_Chr5g0046551
rosa_laevigata	RLG00000034417
rosa_multiflora	Rmu_sc0003878.1_g000038
rosa_roxburghii	Rroxscaffold_1G00034850
rosa_rugosa	Rorug05G0229500
rosa_samantha	Rh5AG309800 Rh5BG318200 Rh5CG343900 Rh5DG328500
rosa_wichuraiana	Rw5G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	11
AciI	CCGC	2 cut(s)	29, 35
AclWI	GGATC	5 cut(s)	326, 826, 839, 1319, 1332
AcoI	YGGCCR	3 cut(s)	353, 1214, 1402
AcsI	RAATTY	4 cut(s)	160, 511, 548, 720
AcuI	CTGAAG	2 cut(s)	213, 750
AfaI	GTAC	1 cut(s)	817
AfiI	CCNNNNNNNGG	4 cut(s)	562, 795, 1016, 1088
AflII	CTTAAG	1 cut(s)	506
AgsI	TTSAA	7 cut(s)	175, 404, 628, 1086, 1273, 1356, 1440
AjnI	CCWGG	1 cut(s)	555
AjuI	GAANNNNNNNTTGG	4 cut(s)	860, 892, 968, 1000
AluBI	AGCT	5 cut(s)	393, 400, 636, 1276, 1375
AluI	AGCT	5 cut(s)	393, 400, 636, 1276, 1375
Alw21I	GWGCWC	1 cut(s)	582
Alw26I	GTCTC	4 cut(s)	536, 881, 1256, 1368
AlwI	GGATC	5 cut(s)	326, 826, 839, 1319, 1332
AlwNI	CAGNNNCTG	1 cut(s)	272
AoxI	GGCC	8 cut(s)	268, 353, 425, 905, 1018, 1137, 1214, 1402
ApoI	RAATTY	4 cut(s)	160, 511, 548, 720
ArsI	GACNNNNNNTTYG	2 cut(s)	451, 483
AspLEI	GCGC	1 cut(s)	1453
AspS9I	GGNCC	4 cut(s)	223, 269, 472, 1019
AsuC2I	CCSGG	1 cut(s)	217
AsuHPI	GGTGA	4 cut(s)	18, 330, 545, 734
AvaII	GGWCC	2 cut(s)	223, 472
BalI	TGGCCA	3 cut(s)	355, 1216, 1404
BamHI	GGATCC	2 cut(s)	831, 1324
BbsI	GAAGAC	1 cut(s)	272
Bbv12I	GWGCWC	1 cut(s)	582
BbvCI	CCTCAGC	1 cut(s)	1134
BccI	CCATC	4 cut(s)	123, 514, 608, 798
BciT130I	CCWGG	1 cut(s)	557
BciVI	GTATCC	1 cut(s)	1026
BclI	TGATCA	2 cut(s)	252, 1170
BcnI	CCSGG	1 cut(s)	217
BcoDI	GTCTC	4 cut(s)	536, 881, 1256, 1368
BfaI	CTAG	7 cut(s)	231, 828, 1118, 1293, 1422, 1478, 1484
BfrI	CTTAAG	1 cut(s)	506
BfuI	GTATCC	1 cut(s)	1026
BlpI	GCTNAGC	1 cut(s)	291
Bme1390I	CCNGG	2 cut(s)	217, 557
Bme18I	GGWCC	2 cut(s)	223, 472
BmgT120I	GGNCC	4 cut(s)	223, 269, 472, 1019
BmiI	GGNNCC	4 cut(s)	225, 695, 833, 1326
BmrFI	CCNGG	2 cut(s)	217, 557
BmsI	GCATC	5 cut(s)	92, 321, 637, 753, 1297
BpiI	GAAGAC	1 cut(s)	272
BplI	GAGNNNNNCTC	2 cut(s)	1378, 1410
Bpu10I	CCTNAGC	2 cut(s)	953, 1134
Bpu1102I	GCTNAGC	1 cut(s)	291
BpuEI	CTTGAG	1 cut(s)	106
BpuMI	CCSGG	1 cut(s)	217
BsaBI	GATNNNNATC	2 cut(s)	257, 789
BsaJI	CCNNGG	6 cut(s)	215, 689, 697, 1028, 1153, 1455
BsaXI	ACNNNNNCTCC	2 cut(s)	1253, 1283
Bsc4I	CCNNNNNNNGG	4 cut(s)	562, 795, 1016, 1088
Bse118I	RCCGGY	1 cut(s)	234
Bse1I	ACTGG	8 cut(s)	66, 87, 651, 823, 900, 908, 1093, 1340
Bse3DI	GCAATG	2 cut(s)	858, 1140
Bse8I	GATNNNNATC	2 cut(s)	257, 789
BseBI	CCWGG	1 cut(s)	557
BseDI	CCNNGG	6 cut(s)	215, 689, 697, 1028, 1153, 1455
BseGI	GGATG	1 cut(s)	83
BseJI	GATNNNNATC	2 cut(s)	257, 789
BseLI	CCNNNNNNNGG	4 cut(s)	562, 795, 1016, 1088
BseMI	GCAATG	2 cut(s)	858, 1140
BseMII	CTCAG	2 cut(s)	282, 1125
BseNI	ACTGG	8 cut(s)	66, 87, 651, 823, 900, 908, 1093, 1340
BseRI	GAGGAG	1 cut(s)	1240
BshFI	GGCC	8 cut(s)	270, 355, 427, 907, 1020, 1139, 1216, 1404
BsiHKAI	GWGCWC	1 cut(s)	582
BsiSI	CCGG	2 cut(s)	217, 235
BslFI	GGGAC	2 cut(s)	1075, 1303
BslI	CCNNNNNNNGG	4 cut(s)	562, 795, 1016, 1088
BsmAI	GTCTC	4 cut(s)	536, 881, 1256, 1368
BsmFI	GGGAC	2 cut(s)	1075, 1303
BsmI	GAATGC	1 cut(s)	966
BsnI	GGCC	8 cut(s)	270, 355, 427, 907, 1020, 1139, 1216, 1404
Bsp1286I	GDGCHC	1 cut(s)	582
Bsp143I	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
Bsp1720I	GCTNAGC	1 cut(s)	291
Bsp19I	CCATGG	1 cut(s)	697
BspACI	CCGC	2 cut(s)	29, 35
BspANI	GGCC	8 cut(s)	270, 355, 427, 907, 1020, 1139, 1216, 1404
BspCNI	CTCAG	2 cut(s)	283, 1126
BspLI	GGNNCC	4 cut(s)	225, 695, 833, 1326
BspPI	GGATC	5 cut(s)	326, 826, 839, 1319, 1332
BspTI	CTTAAG	1 cut(s)	506
BsrDI	GCAATG	2 cut(s)	858, 1140
BsrFI	RCCGGY	1 cut(s)	234
BsrI	ACTGG	8 cut(s)	66, 87, 651, 823, 900, 908, 1093, 1340
BssAI	RCCGGY	1 cut(s)	234
BssECI	CCNNGG	6 cut(s)	215, 689, 697, 1028, 1153, 1455
BssMI	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
BssT1I	CCWWGG	4 cut(s)	689, 697, 1153, 1455
Bst2UI	CCWGG	1 cut(s)	557
Bst4CI	ACNGT	2 cut(s)	306, 476
Bst6I	CTCTTC	4 cut(s)	879, 971, 1029, 1357
BstAFI	CTTAAG	1 cut(s)	506
BstC8I	GCNNGC	3 cut(s)	634, 664, 1377
BstDEI	CTNAG	7 cut(s)	291, 412, 637, 871, 953, 1134, 1367
BstDSI	CCRYGG	1 cut(s)	697
BstEII	GGTNACC	1 cut(s)	740
BstF5I	GGATG	1 cut(s)	83
BstHHI	GCGC	1 cut(s)	1453
BstKTI	GATC	7 cut(s)	211, 255, 334, 834, 1173, 1282, 1327
BstMAI	GTCTC	4 cut(s)	536, 881, 1256, 1368
BstMBI	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
BstNI	CCWGG	1 cut(s)	557
BstPI	GGTNACC	1 cut(s)	740
BstSCI	CCNGG	2 cut(s)	215, 555
BstV2I	GAAGAC	1 cut(s)	272
BstX2I	RGATCY	2 cut(s)	831, 1324
BstXI	CCANNNNNNTGG	1 cut(s)	469
BstYI	RGATCY	2 cut(s)	831, 1324
BsuI	GTATCC	1 cut(s)	1026
BsuRI	GGCC	8 cut(s)	270, 355, 427, 907, 1020, 1139, 1216, 1404
BtgI	CCRYGG	1 cut(s)	697
BtsCI	GGATG	1 cut(s)	83
BtsIMutI	CAGTG	2 cut(s)	658, 893
Cac8I	GCNNGC	3 cut(s)	634, 664, 1377
CaiI	CAGNNNCTG	1 cut(s)	272
CfoI	GCGC	1 cut(s)	1453
Cfr10I	RCCGGY	1 cut(s)	234
Cfr13I	GGNCC	4 cut(s)	223, 269, 472, 1019
Csp6I	GTAC	1 cut(s)	816
CspCI	CAANNNNNGTGG	2 cut(s)	1181, 1216
CviAII	CATG	8 cut(s)	80, 423, 698, 716, 922, 1096, 1406, 1492
CviQI	GTAC	1 cut(s)	816
DdeI	CTNAG	7 cut(s)	291, 412, 637, 871, 953, 1134, 1367
DpnI	GATC	7 cut(s)	210, 254, 333, 833, 1172, 1281, 1326
DpnII	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
EaeI	YGGCCR	3 cut(s)	353, 1214, 1402
Eam1104I	CTCTTC	4 cut(s)	879, 971, 1029, 1357
EarI	CTCTTC	4 cut(s)	879, 971, 1029, 1357
Eco130I	CCWWGG	4 cut(s)	689, 697, 1153, 1455
Eco47I	GGWCC	2 cut(s)	223, 472
Eco57I	CTGAAG	2 cut(s)	213, 750
Eco91I	GGTNACC	1 cut(s)	740
EcoO109I	RGGNCCY	2 cut(s)	269, 1019
EcoO65I	GGTNACC	1 cut(s)	740
EcoRII	CCWGG	1 cut(s)	555
EcoT14I	CCWWGG	4 cut(s)	689, 697, 1153, 1455
EcoT22I	ATGCAT	1 cut(s)	85
ErhI	CCWWGG	4 cut(s)	689, 697, 1153, 1455
FaeI	CATG	8 cut(s)	83, 426, 701, 719, 925, 1099, 1409, 1495
FalI	AAGNNNNNCTT	2 cut(s)	620, 652
FaqI	GGGAC	2 cut(s)	1075, 1303
FatI	CATG	8 cut(s)	79, 422, 697, 715, 921, 1095, 1405, 1491
FbaI	TGATCA	2 cut(s)	252, 1170
FokI	GGATG	1 cut(s)	70
FspBI	CTAG	7 cut(s)	231, 828, 1118, 1293, 1422, 1478, 1484
GlaI	GCGC	1 cut(s)	1452
HaeIII	GGCC	8 cut(s)	270, 355, 427, 907, 1020, 1139, 1216, 1404
HapII	CCGG	2 cut(s)	217, 235
HhaI	GCGC	1 cut(s)	1453
Hin1II	CATG	8 cut(s)	83, 426, 701, 719, 925, 1099, 1409, 1495
Hin6I	GCGC	1 cut(s)	1451
HinP1I	GCGC	1 cut(s)	1451
HincII	GTYRAC	1 cut(s)	71
HindII	GTYRAC	1 cut(s)	71
HindIII	AAGCTT	2 cut(s)	634, 1274
HinfI	GANTC	9 cut(s)	141, 182, 489, 543, 593, 785, 863, 1006, 1474
HpaII	CCGG	2 cut(s)	217, 235
HphI	GGTGA	4 cut(s)	18, 330, 545, 734
Hpy166II	GTNNAC	2 cut(s)	71, 1078
Hpy188I	TCNGA	6 cut(s)	17, 247, 708, 1032, 1107, 1175
Hpy188III	TCNNGA	3 cut(s)	123, 138, 167
Hpy8I	GTNNAC	2 cut(s)	71, 1078
HpyAV	CCTTC	6 cut(s)	237, 345, 622, 757, 1092, 1290
HpyCH4III	ACNGT	2 cut(s)	306, 476
HpyCH4V	TGCA	4 cut(s)	83, 662, 851, 1145
HpyF3I	CTNAG	7 cut(s)	291, 412, 637, 871, 953, 1134, 1367
Hsp92II	CATG	8 cut(s)	83, 426, 701, 719, 925, 1099, 1409, 1495
HspAI	GCGC	1 cut(s)	1451
Ksp22I	TGATCA	2 cut(s)	252, 1170
Kzo9I	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
LmnI	GCTCC	1 cut(s)	397
LweI	GCATC	5 cut(s)	92, 321, 637, 753, 1297
MaeI	CTAG	7 cut(s)	231, 828, 1118, 1293, 1422, 1478, 1484
MaeIII	GTNAC	3 cut(s)	300, 740, 1091
MalI	GATC	7 cut(s)	210, 254, 333, 833, 1172, 1281, 1326
MboI	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
MboII	GAAGA	6 cut(s)	272, 511, 896, 988, 1016, 1344
MfeI	CAATTG	2 cut(s)	1053, 1218
MflI	RGATCY	2 cut(s)	831, 1324
MhlI	GDGCHC	1 cut(s)	582
MlsI	TGGCCA	3 cut(s)	355, 1216, 1404
MluCI	AATT	9 cut(s)	160, 511, 548, 573, 667, 720, 823, 1053, 1218
MluNI	TGGCCA	3 cut(s)	355, 1216, 1404
MlyI	GAGTC	5 cut(s)	150, 191, 498, 552, 602
MmeI	TCCRAC	2 cut(s)	201, 891
Mox20I	TGGCCA	3 cut(s)	355, 1216, 1404
Mph1103I	ATGCAT	1 cut(s)	85
MscI	TGGCCA	3 cut(s)	355, 1216, 1404
MseI	TTAA	4 cut(s)	507, 930, 1284, 1461
MslI	CAYNNNNRTG	2 cut(s)	447, 971
Msp20I	TGGCCA	3 cut(s)	355, 1216, 1404
MspCI	CTTAAG	1 cut(s)	506
MspI	CCGG	2 cut(s)	217, 235
MspR9I	CCNGG	2 cut(s)	217, 557
MunI	CAATTG	2 cut(s)	1053, 1218
Mva1269I	GAATGC	1 cut(s)	966
MvaI	CCWGG	1 cut(s)	557
NciI	CCSGG	1 cut(s)	217
NcoI	CCATGG	1 cut(s)	697
NdeII	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
NlaIII	CATG	8 cut(s)	83, 426, 701, 719, 925, 1099, 1409, 1495
NlaIV	GGNNCC	4 cut(s)	225, 695, 833, 1326
NmuCI	GTSAC	2 cut(s)	300, 740
NsiI	ATGCAT	1 cut(s)	85
PctI	GAATGC	1 cut(s)	966
PfeI	GAWTC	4 cut(s)	785, 863, 1006, 1474
PleI	GAGTC	5 cut(s)	149, 190, 497, 551, 601
PpsI	GAGTC	5 cut(s)	149, 190, 497, 551, 601
Psp6I	CCWGG	1 cut(s)	555
PspEI	GGTNACC	1 cut(s)	740
PspGI	CCWGG	1 cut(s)	555
PspN4I	GGNNCC	4 cut(s)	225, 695, 833, 1326
PspPI	GGNCC	4 cut(s)	223, 269, 472, 1019
PstNI	CAGNNNCTG	1 cut(s)	272
PsuI	RGATCY	2 cut(s)	831, 1324
RsaI	GTAC	1 cut(s)	817
RsaNI	GTAC	1 cut(s)	816
RseI	CAYNNNNRTG	2 cut(s)	447, 971
SaqAI	TTAA	4 cut(s)	507, 930, 1284, 1461
Sau3AI	GATC	7 cut(s)	208, 252, 331, 831, 1170, 1279, 1324
Sau96I	GGNCC	4 cut(s)	223, 269, 472, 1019
SchI	GAGTC	5 cut(s)	150, 191, 498, 552, 602
ScrFI	CCNGG	2 cut(s)	217, 557
SduI	GDGCHC	1 cut(s)	582
SfaNI	GCATC	5 cut(s)	92, 321, 637, 753, 1297
SinI	GGWCC	2 cut(s)	223, 472
SmiMI	CAYNNNNRTG	2 cut(s)	447, 971
SmlI	CTYRAG	2 cut(s)	121, 506
SmoI	CTYRAG	2 cut(s)	121, 506
Sse9I	AATT	9 cut(s)	160, 511, 548, 573, 667, 720, 823, 1053, 1218
SsiI	CCGC	2 cut(s)	29, 35
SspI	AATATT	1 cut(s)	1244
SspMI	CTAG	7 cut(s)	231, 828, 1118, 1293, 1422, 1478, 1484
StyD4I	CCNGG	2 cut(s)	215, 555
StyI	CCWWGG	4 cut(s)	689, 697, 1153, 1455
TaaI	ACNGT	2 cut(s)	306, 476
TaqI	TCGA	5 cut(s)	487, 750, 970, 1160, 1428
TaqII	GACCGA	1 cut(s)	231
TasI	AATT	9 cut(s)	160, 511, 548, 573, 667, 720, 823, 1053, 1218
TfiI	GAWTC	4 cut(s)	785, 863, 1006, 1474
Tru1I	TTAA	4 cut(s)	507, 930, 1284, 1461
Tru9I	TTAA	4 cut(s)	507, 930, 1284, 1461
TscAI	CASTG	2 cut(s)	658, 900
TseFI	GTSAC	2 cut(s)	300, 740
Tsp45I	GTSAC	2 cut(s)	300, 740
TspDTI	ATGAA	4 cut(s)	68, 431, 816, 1178
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	2 cut(s)	658, 900
Vha464I	CTTAAG	1 cut(s)	506
VpaK11BI	GGWCC	2 cut(s)	223, 472
XapI	RAATTY	4 cut(s)	160, 511, 548, 720
XcmI	CCANNNNNNNNNTGG	2 cut(s)	152, 1085
XspI	CTAG	7 cut(s)	231, 828, 1118, 1293, 1422, 1478, 1484
Zsp2I	ATGCAT	1 cut(s)	85

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.