Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0015577

Physical Location & Seq

Genomic Coordinates Reverse (-)

42390347 .. 42391837

Gene Structure 1491 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034417

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGGACACCCTTGTCAGATTGTTTTCACCGCCACCGCCACTACCACTACCAACCAAGACCAGTCAACTCTTTCATGCATCCAGTCCTCCATTTTCTTTGCCAAAACCCCATCACTCAAGAAAGAGATTTTCCAGAGTCCAAAGCAAGTATGGCAATTTTCTGGACTTGAAACCAGAGTCAAAACCTGAAGTGCTAGACTTTGATCTGCCCCGGTTGGACCCTTCTAGCCGGTTTCGGTCAGATGTGATCATCATCGGTACAGGCCCTGCTGGTCTTCGTCTTGCTGAGCAAGTGTCACAGTATGGCATCAAGGTATGTTGTGTTGATCCTTCACCACTTTCTATGTGGCCAAATAACTATGGTGTTTGGGTTGATGAGTTTGAGAGCTTGGAGCTTGAAAGTTGCTTAGACAAAACATGGCCTATGGCTTGTGTTCATATTGATGATAGTAAGACCAAGTATTTGGACCGTCCTTATGGTCGAGTCAGTAGGAAGAAACTTAAGAATTTGTTGATGGAGAGGTGTCTCTCCAATGGAGTCAAATTTCACCAGGCAAAGGTTTGGAAAATTGAGCACAAAGAGTTTGAGTCTTTGATTTTGTGTGATGATGGGAATGAGTTGAAGGCAAGCTTAGTTGTTGATGCCAGTGGTTTTGCAAGCAATTTCGTAGAGTATAATAAGCCAAGGAACCATGGCTATCAGATTGCTCATGGAATTTTGGCTGAAGTGGAGGGTCACCCCTTCGACTTGGACAAGATGCTTCTTATGGATTGGAGGGATTCCCATCTTGGGAATGAACCTTACTTGCGTACTGGTAATTCTAGGATCCCCACTTTTCTGTATGCAATGCCATTTGATTCCAACTTAGTATTCTTGGAAGAGACTTCACTGGTTAGTAGGCCAGTTTTATCTTACATGGAGATTAAGAAAAGAATGGTGGCGAGGCTAAGGCATTTGGGCATTCGAGTGAAGAGGATATTGGAGGTTGAGAAGTGTTTGATTCCAATGGGAGGCCCTCTTCCTCGGATACCTCAAAGCGTTATGGCAATGGGAGGGACTTCTGGGATTGTCCACCCTTCAACTGGTTACATGGTGGCTCGGACAATGGCTCTAGCACCGATACTTGCTGTGGCCATTGCAGAGTGCCTTGGTTCGAGCCGAATGATCAGAGGGAAATCGCTTCATCAACGAGCGTGGAATGGTTTGTGGCCAATTGAGAGGAGATGTGTAAGAGAATATTACTCTTTCGGGATGGAGACCTTGTTGAAGCTTGATCTTAATGGGACTAGAAGGTTTTTTGATGCTTTCTTTGACTTGGATCCTTATTACTGGCAAGGGTTTCTCTCTTCAAGGTTGTCTCTCAGAGAGCTTGTCTTGTTGAGTTTATCTCTGTTTGGCCATGCCTCATTTCCATCTAGATTCGATATTGTTTCAAAGTGTCCTGCGCCCTTGGTTAAAATGATTGGGAATCTAGCACTAGAAACCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006066 GO:0006082 GO:0006629 GO:0006714 GO:0006720 GO:0006721 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009687 GO:0009688 GO:0009987 GO:0016053 GO:0016106 GO:0016108 GO:0016109 GO:0016114 GO:0016116 GO:0016117 GO:0016122 GO:0016123 GO:0016853 GO:0016860 GO:0019752 GO:0032787 GO:0034020 GO:0042440 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043288 GO:0043289 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046165 GO:0046394 GO:0071704 GO:0072330 GO:1901576 GO:1901615 GO:1901617 GO:1902644 GO:1902645

KEGG Pathways

Metabolic & Signaling

ko00906 ko01100 ko01110 map00906 map01100 map01110

Pfam Domains

Protein Families

Lycopene_cycl Trp_halogenase

Protein Analysis

497

Amino Acids

56.37

Weight (kDa)

9.07

Isoelectric Point (pI)

47.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lycopene_cycl	PF05834	81 - 473	4.2e-146	Lycopene cyclase protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015577)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g25490
malus_domestica	MD03G1148700.v1.1 MD11G1168000.v1.1
prunus_persica	Prupe.6G135000_v2.0.a1
pyrus_communis	pycom03g10310 pycom11g14030
rosa_chinensis	RchiOBHm_Chr5g0046551
rosa_laevigata	RLG00000034417
rosa_multiflora	Rmu_sc0003878.1_g000038
rosa_roxburghii	Rroxscaffold_1G00034850
rosa_rugosa	Rorug05G0229500
rosa_samantha	Rh5AG309800 Rh5BG318200 Rh5CG343900 Rh5DG328500
rosa_wichuraiana	Rw5G028900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	11
AciI	CCGC	2 cut(s)	29, 35
AclWI	GGATC	5 cut(s)	320, 820, 833, 1313, 1326
AcoI	YGGCCR	4 cut(s)	347, 1131, 1208, 1396
AcsI	RAATTY	3 cut(s)	505, 542, 714
AcuI	CTGAAG	2 cut(s)	207, 744
AfaI	GTAC	2 cut(s)	259, 811
AfiI	CCNNNNNNNGG	4 cut(s)	556, 789, 1010, 1082
AflII	CTTAAG	1 cut(s)	500
AgsI	TTSAA	7 cut(s)	169, 398, 622, 1080, 1267, 1350, 1434
AjnI	CCWGG	1 cut(s)	549
AjuI	GAANNNNNNNTTGG	4 cut(s)	854, 886, 962, 994
AluBI	AGCT	5 cut(s)	387, 394, 630, 1270, 1369
AluI	AGCT	5 cut(s)	387, 394, 630, 1270, 1369
Alw21I	GWGCWC	1 cut(s)	576
Alw26I	GTCTC	4 cut(s)	530, 875, 1250, 1362
AlwI	GGATC	5 cut(s)	320, 820, 833, 1313, 1326
AlwNI	CAGNNNCTG	1 cut(s)	266
AoxI	GGCC	8 cut(s)	262, 347, 419, 899, 1012, 1131, 1208, 1396
ApoI	RAATTY	3 cut(s)	505, 542, 714
ArsI	GACNNNNNNTTYG	2 cut(s)	445, 477
AspLEI	GCGC	1 cut(s)	1447
AspS9I	GGNCC	4 cut(s)	217, 263, 466, 1013
AsuC2I	CCSGG	1 cut(s)	211
AsuHPI	GGTGA	4 cut(s)	18, 324, 539, 728
AvaII	GGWCC	2 cut(s)	217, 466
BalI	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
BamHI	GGATCC	2 cut(s)	825, 1318
BbsI	GAAGAC	1 cut(s)	266
Bbv12I	GWGCWC	1 cut(s)	576
BccI	CCATC	6 cut(s)	117, 508, 602, 792, 1246, 1420
BciT130I	CCWGG	1 cut(s)	551
BciVI	GTATCC	1 cut(s)	1020
BclI	TGATCA	2 cut(s)	246, 1164
BcnI	CCSGG	1 cut(s)	211
BcoDI	GTCTC	4 cut(s)	530, 875, 1250, 1362
BfaI	CTAG	8 cut(s)	194, 225, 822, 1112, 1287, 1416, 1472, 1478
BfrI	CTTAAG	1 cut(s)	500
BfuI	GTATCC	1 cut(s)	1020
BlpI	GCTNAGC	1 cut(s)	285
Bme1390I	CCNGG	2 cut(s)	211, 551
Bme18I	GGWCC	2 cut(s)	217, 466
BmgT120I	GGNCC	4 cut(s)	217, 263, 466, 1013
BmiI	GGNNCC	4 cut(s)	219, 689, 827, 1320
BmrFI	CCNGG	2 cut(s)	211, 551
BmsI	GCATC	5 cut(s)	86, 315, 631, 747, 1291
BpiI	GAAGAC	1 cut(s)	266
BplI	GAGNNNNNCTC	2 cut(s)	1372, 1404
Bpu10I	CCTNAGC	1 cut(s)	947
Bpu1102I	GCTNAGC	1 cut(s)	285
BpuEI	CTTGAG	1 cut(s)	100
BpuMI	CCSGG	1 cut(s)	211
BsaBI	GATNNNNATC	2 cut(s)	251, 783
BsaI	GGTCTC	1 cut(s)	1250
BsaJI	CCNNGG	6 cut(s)	209, 683, 691, 1022, 1147, 1449
BsaXI	ACNNNNNCTCC	2 cut(s)	1247, 1277
Bsc4I	CCNNNNNNNGG	4 cut(s)	556, 789, 1010, 1082
Bse118I	RCCGGY	1 cut(s)	228
Bse1I	ACTGG	8 cut(s)	60, 81, 645, 817, 894, 902, 1087, 1334
Bse3DI	GCAATG	3 cut(s)	852, 1053, 1134
Bse8I	GATNNNNATC	2 cut(s)	251, 783
BseBI	CCWGG	1 cut(s)	551
BseDI	CCNNGG	6 cut(s)	209, 683, 691, 1022, 1147, 1449
BseGI	GGATG	2 cut(s)	77, 1257
BseJI	GATNNNNATC	2 cut(s)	251, 783
BseLI	CCNNNNNNNGG	4 cut(s)	556, 789, 1010, 1082
BseMI	GCAATG	3 cut(s)	852, 1053, 1134
BseMII	CTCAG	2 cut(s)	276, 1375
BseNI	ACTGG	8 cut(s)	60, 81, 645, 817, 894, 902, 1087, 1334
BseRI	GAGGAG	1 cut(s)	1234
BshFI	GGCC	8 cut(s)	264, 349, 421, 901, 1014, 1133, 1210, 1398
BsiHKAI	GWGCWC	1 cut(s)	576
BsiSI	CCGG	2 cut(s)	211, 229
BslFI	GGGAC	2 cut(s)	1069, 1297
BslI	CCNNNNNNNGG	4 cut(s)	556, 789, 1010, 1082
BsmAI	GTCTC	4 cut(s)	530, 875, 1250, 1362
BsmFI	GGGAC	2 cut(s)	1069, 1297
BsmI	GAATGC	1 cut(s)	960
BsnI	GGCC	8 cut(s)	264, 349, 421, 901, 1014, 1133, 1210, 1398
Bso31I	GGTCTC	1 cut(s)	1250
Bsp1286I	GDGCHC	1 cut(s)	576
Bsp143I	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
Bsp1720I	GCTNAGC	1 cut(s)	285
Bsp19I	CCATGG	1 cut(s)	691
BspACI	CCGC	2 cut(s)	29, 35
BspANI	GGCC	8 cut(s)	264, 349, 421, 901, 1014, 1133, 1210, 1398
BspCNI	CTCAG	2 cut(s)	277, 1374
BspLI	GGNNCC	4 cut(s)	219, 689, 827, 1320
BspPI	GGATC	5 cut(s)	320, 820, 833, 1313, 1326
BspTI	CTTAAG	1 cut(s)	500
BspTNI	GGTCTC	1 cut(s)	1250
BsrDI	GCAATG	3 cut(s)	852, 1053, 1134
BsrFI	RCCGGY	1 cut(s)	228
BsrI	ACTGG	8 cut(s)	60, 81, 645, 817, 894, 902, 1087, 1334
BssAI	RCCGGY	1 cut(s)	228
BssECI	CCNNGG	6 cut(s)	209, 683, 691, 1022, 1147, 1449
BssMI	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
BssT1I	CCWWGG	4 cut(s)	683, 691, 1147, 1449
Bst2UI	CCWGG	1 cut(s)	551
Bst4CI	ACNGT	2 cut(s)	300, 470
Bst6I	CTCTTC	4 cut(s)	873, 965, 1023, 1351
BstAFI	CTTAAG	1 cut(s)	500
BstC8I	GCNNGC	2 cut(s)	628, 658
BstDEI	CTNAG	6 cut(s)	285, 406, 631, 865, 947, 1361
BstDSI	CCRYGG	1 cut(s)	691
BstEII	GGTNACC	1 cut(s)	734
BstF5I	GGATG	2 cut(s)	77, 1257
BstHHI	GCGC	1 cut(s)	1447
BstKTI	GATC	7 cut(s)	205, 249, 328, 828, 1167, 1276, 1321
BstMAI	GTCTC	4 cut(s)	530, 875, 1250, 1362
BstMBI	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
BstMWI	GCNNNNNNNGC	1 cut(s)	150
BstNI	CCWGG	1 cut(s)	551
BstPI	GGTNACC	1 cut(s)	734
BstSCI	CCNGG	2 cut(s)	209, 549
BstV2I	GAAGAC	1 cut(s)	266
BstX2I	RGATCY	2 cut(s)	825, 1318
BstXI	CCANNNNNNTGG	1 cut(s)	463
BstYI	RGATCY	2 cut(s)	825, 1318
BsuI	GTATCC	1 cut(s)	1020
BsuRI	GGCC	8 cut(s)	264, 349, 421, 901, 1014, 1133, 1210, 1398
BtgI	CCRYGG	1 cut(s)	691
BtsCI	GGATG	2 cut(s)	77, 1257
BtsIMutI	CAGTG	2 cut(s)	652, 887
Cac8I	GCNNGC	2 cut(s)	628, 658
CaiI	CAGNNNCTG	1 cut(s)	266
CfoI	GCGC	1 cut(s)	1447
Cfr10I	RCCGGY	1 cut(s)	228
Cfr13I	GGNCC	4 cut(s)	217, 263, 466, 1013
Csp6I	GTAC	2 cut(s)	258, 810
CspCI	CAANNNNNGTGG	2 cut(s)	1175, 1210
CviAII	CATG	8 cut(s)	74, 417, 692, 710, 916, 1090, 1400, 1486
CviQI	GTAC	2 cut(s)	258, 810
DdeI	CTNAG	6 cut(s)	285, 406, 631, 865, 947, 1361
DpnI	GATC	7 cut(s)	204, 248, 327, 827, 1166, 1275, 1320
DpnII	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
DrdI	GACNNNNNNGTC	1 cut(s)	11
DseDI	GACNNNNNNGTC	1 cut(s)	11
EaeI	YGGCCR	4 cut(s)	347, 1131, 1208, 1396
Eam1104I	CTCTTC	4 cut(s)	873, 965, 1023, 1351
EarI	CTCTTC	4 cut(s)	873, 965, 1023, 1351
Eco130I	CCWWGG	4 cut(s)	683, 691, 1147, 1449
Eco31I	GGTCTC	1 cut(s)	1250
Eco47I	GGWCC	2 cut(s)	217, 466
Eco57I	CTGAAG	2 cut(s)	207, 744
Eco91I	GGTNACC	1 cut(s)	734
EcoO109I	RGGNCCY	2 cut(s)	263, 1013
EcoO65I	GGTNACC	1 cut(s)	734
EcoRII	CCWGG	1 cut(s)	549
EcoT14I	CCWWGG	4 cut(s)	683, 691, 1147, 1449
EcoT22I	ATGCAT	1 cut(s)	79
ErhI	CCWWGG	4 cut(s)	683, 691, 1147, 1449
FaeI	CATG	8 cut(s)	77, 420, 695, 713, 919, 1093, 1403, 1489
FalI	AAGNNNNNCTT	2 cut(s)	614, 646
FaqI	GGGAC	2 cut(s)	1069, 1297
FatI	CATG	8 cut(s)	73, 416, 691, 709, 915, 1089, 1399, 1485
FbaI	TGATCA	2 cut(s)	246, 1164
FokI	GGATG	2 cut(s)	64, 1264
FspBI	CTAG	8 cut(s)	194, 225, 822, 1112, 1287, 1416, 1472, 1478
GlaI	GCGC	1 cut(s)	1446
HaeIII	GGCC	8 cut(s)	264, 349, 421, 901, 1014, 1133, 1210, 1398
HapII	CCGG	2 cut(s)	211, 229
HhaI	GCGC	1 cut(s)	1447
Hin1II	CATG	8 cut(s)	77, 420, 695, 713, 919, 1093, 1403, 1489
Hin6I	GCGC	1 cut(s)	1445
HinP1I	GCGC	1 cut(s)	1445
HincII	GTYRAC	1 cut(s)	65
HindII	GTYRAC	1 cut(s)	65
HindIII	AAGCTT	2 cut(s)	628, 1268
HpaII	CCGG	2 cut(s)	211, 229
HphI	GGTGA	4 cut(s)	18, 324, 539, 728
Hpy166II	GTNNAC	2 cut(s)	65, 1072
Hpy188I	TCNGA	7 cut(s)	17, 241, 702, 1026, 1101, 1169, 1364
Hpy188III	TCNNGA	5 cut(s)	117, 132, 161, 1249, 1416
Hpy8I	GTNNAC	2 cut(s)	65, 1072
HpyAV	CCTTC	6 cut(s)	231, 339, 616, 751, 1086, 1284
HpyCH4III	ACNGT	2 cut(s)	300, 470
HpyCH4V	TGCA	4 cut(s)	77, 656, 845, 1139
HpyF10VI	GCNNNNNNNGC	1 cut(s)	150
HpyF3I	CTNAG	6 cut(s)	285, 406, 631, 865, 947, 1361
Hsp92II	CATG	8 cut(s)	77, 420, 695, 713, 919, 1093, 1403, 1489
HspAI	GCGC	1 cut(s)	1445
Ksp22I	TGATCA	2 cut(s)	246, 1164
Kzo9I	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
LmnI	GCTCC	1 cut(s)	391
LweI	GCATC	5 cut(s)	86, 315, 631, 747, 1291
MaeI	CTAG	8 cut(s)	194, 225, 822, 1112, 1287, 1416, 1472, 1478
MaeIII	GTNAC	3 cut(s)	294, 734, 1085
MalI	GATC	7 cut(s)	204, 248, 327, 827, 1166, 1275, 1320
MboI	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
MboII	GAAGA	6 cut(s)	266, 505, 890, 982, 1010, 1338
MfeI	CAATTG	1 cut(s)	1212
MflI	RGATCY	2 cut(s)	825, 1318
MhlI	GDGCHC	1 cut(s)	576
MlsI	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
MluCI	AATT	8 cut(s)	154, 505, 542, 567, 661, 714, 817, 1212
MluNI	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
MlyI	GAGTC	5 cut(s)	144, 185, 492, 546, 596
MmeI	TCCRAC	2 cut(s)	195, 885
Mox20I	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
Mph1103I	ATGCAT	1 cut(s)	79
MscI	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
MseI	TTAA	4 cut(s)	501, 924, 1278, 1455
MslI	CAYNNNNRTG	2 cut(s)	441, 965
Msp20I	TGGCCA	4 cut(s)	349, 1133, 1210, 1398
MspCI	CTTAAG	1 cut(s)	500
MspI	CCGG	2 cut(s)	211, 229
MspR9I	CCNGG	2 cut(s)	211, 551
MunI	CAATTG	1 cut(s)	1212
Mva1269I	GAATGC	1 cut(s)	960
MvaI	CCWGG	1 cut(s)	551
MwoI	GCNNNNNNNGC	1 cut(s)	150
NciI	CCSGG	1 cut(s)	211
NcoI	CCATGG	1 cut(s)	691
NdeII	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
NlaIII	CATG	8 cut(s)	77, 420, 695, 713, 919, 1093, 1403, 1489
NlaIV	GGNNCC	4 cut(s)	219, 689, 827, 1320
NmuCI	GTSAC	2 cut(s)	294, 734
NsiI	ATGCAT	1 cut(s)	79
PctI	GAATGC	1 cut(s)	960
PfeI	GAWTC	5 cut(s)	779, 857, 1000, 1419, 1468
PleI	GAGTC	5 cut(s)	143, 184, 491, 545, 595
PpsI	GAGTC	5 cut(s)	143, 184, 491, 545, 595
Psp6I	CCWGG	1 cut(s)	549
PspEI	GGTNACC	1 cut(s)	734
PspGI	CCWGG	1 cut(s)	549
PspN4I	GGNNCC	4 cut(s)	219, 689, 827, 1320
PspPI	GGNCC	4 cut(s)	217, 263, 466, 1013
PstNI	CAGNNNCTG	1 cut(s)	266
PsuI	RGATCY	2 cut(s)	825, 1318
RsaI	GTAC	2 cut(s)	259, 811
RsaNI	GTAC	2 cut(s)	258, 810
RseI	CAYNNNNRTG	2 cut(s)	441, 965
SaqAI	TTAA	4 cut(s)	501, 924, 1278, 1455
Sau3AI	GATC	7 cut(s)	202, 246, 325, 825, 1164, 1273, 1318
Sau96I	GGNCC	4 cut(s)	217, 263, 466, 1013
SchI	GAGTC	5 cut(s)	144, 185, 492, 546, 596
ScrFI	CCNGG	2 cut(s)	211, 551
SduI	GDGCHC	1 cut(s)	576
SfaNI	GCATC	5 cut(s)	86, 315, 631, 747, 1291
SinI	GGWCC	2 cut(s)	217, 466
SmiMI	CAYNNNNRTG	2 cut(s)	441, 965
SmlI	CTYRAG	2 cut(s)	115, 500
SmoI	CTYRAG	2 cut(s)	115, 500
Sse9I	AATT	8 cut(s)	154, 505, 542, 567, 661, 714, 817, 1212
SsiI	CCGC	2 cut(s)	29, 35
SspI	AATATT	1 cut(s)	1238
SspMI	CTAG	8 cut(s)	194, 225, 822, 1112, 1287, 1416, 1472, 1478
StyD4I	CCNGG	2 cut(s)	209, 549
StyI	CCWWGG	4 cut(s)	683, 691, 1147, 1449
TaaI	ACNGT	2 cut(s)	300, 470
TaqI	TCGA	5 cut(s)	481, 744, 964, 1154, 1422
TaqII	GACCGA	1 cut(s)	225
TasI	AATT	8 cut(s)	154, 505, 542, 567, 661, 714, 817, 1212
TfiI	GAWTC	5 cut(s)	779, 857, 1000, 1419, 1468
Tru1I	TTAA	4 cut(s)	501, 924, 1278, 1455
Tru9I	TTAA	4 cut(s)	501, 924, 1278, 1455
TscAI	CASTG	2 cut(s)	652, 894
TseFI	GTSAC	2 cut(s)	294, 734
Tsp45I	GTSAC	2 cut(s)	294, 734
TspDTI	ATGAA	4 cut(s)	62, 425, 810, 1172
TspRI	CASTG	2 cut(s)	652, 894
Vha464I	CTTAAG	1 cut(s)	500
VpaK11BI	GGWCC	2 cut(s)	217, 466
XapI	RAATTY	3 cut(s)	505, 542, 714
XbaI	TCTAGA	1 cut(s)	1415
XcmI	CCANNNNNNNNNTGG	3 cut(s)	146, 342, 1079
XspI	CTAG	8 cut(s)	194, 225, 822, 1112, 1287, 1416, 1472, 1478
Zsp2I	ATGCAT	1 cut(s)	79

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.