FvH4_3g27200

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014698
Physical Location & Seq
Forward (+)
20300784 .. 20301983
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27200.t1

Sequence Viewer

Length: 1200 bp
ATGAAGCAGAAGAAACATATAGATGCTGATTGGGCAGGTCTGCCTGACAACATTCTTGAGGTAATCTTTGAGCGACTGAATTTGATGGATTGTATGTCTATTAGTGATGTTTGCAAATCTTGGAGACGTGTTGTTGCACAAGAACTTTCTGATTGTCAAGGGCATGGGTTCCCGTGGCTCCTAATGTCTGGTGAGAAAGACAAAAGGGTTAGGACTTGTATTAGCATATTGCAAGAGCAAGAGTGGGAGATGTTGCTTCCGGAGGCGTATGGTAGATACTGCTGGGGATCATATCAAGAGTGGTTGATTCTAGTTAGAGATCTTGGCTCTTACAAGCTTGATATTGGATTGTTTAACCCATTCACTCGGAAGCAAGTTGATCTTCCTGGAGAATGGAACTTGAATCACAAGATGGTTCTCTCAGGGCCTCCCTCTCTTGAAAACTGTATCTTCATGCTGATTCAAAGTGACTCCCAGACGCTTGCCTTTTGGGTTCCAGGAGCTCAATCATGGCTTCAGCATAAGCTTGATGGTGAACCATTTGTGGATGGTGTCTTCTCCAATGGAAGTTTTTATCTCATCGGCAACGATTATAACATATGTGAAATTAAAGGTGCCAATATATTTGCTGCAATTAAGAGAGACGATGCCTCTGCTCCTGTTGCTTTTCATATTGAAAGGCATTACCATGAAGTAACAATGCCAGATGAGCATGAAAATAATGGCGTGCTGAAGTATCTTGTTGAATCTTGTGGAGAGCTTCTACTTGTCTGCAGGTTCTATAGCACCAAACCAGATGCTGTGCTTGTAACACATTATTTTGAGGTATACTCGCTGGATTTTAGCAGGATGTCCTGGAAGAGGGTAGAGAGTTTGGGAGATCAGATCCTATTTCTGGGCAAGTGCTGTTCAAGATCTGTCTCTTCTAGTGGACTTGGACTTAATGTGACTGATCGTATCTATTTCTCAAATGATCATGCTGTTCCATGGTGGAACGAATGGGATTCTGAACATCTTGTTGGCATTTTGAATCGTCTTGGTCTTGACAATAGTGGGACAAAGGATTGGGGCATTTTTAGCCTCGGCAACAAGAATCATGAAACTTTGTCTATCCAGTTCCGTGGCAATCGTGACAGATGGGGACCTGTCTGGTTTACTGCACCGCAATGGTGGTGTTGTAAGAATTTCACTCTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

400

Amino Acids

46.02

Weight (kDa)

5.49

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 11 - 48 9.3e-07 F-box-like
F-box PF00646 12 - 46 7.1e-08 F-box domain
Beta-prop_KIB1-4 PF03478 84 - 344 8.2e-46 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27200
malus_domestica MD13G1035900.v1.1
prunus_persica Prupe.6G174300_v2.0.a1 Prupe.6G178100_v2.0.a1
pyrus_communis pycom13g03130 pycom16g03310
rosa_chinensis RchiOBHm_Chr4g0434481
rosa_laevigata RLG00000006668
rosa_multiflora Rmu_sc0002251.1_g000016 Rmu_sc0005899.1_g000009
rosa_roxburghii Rroxscaffold_5G00375830
rosa_rugosa Rorug04G0277000
rosa_samantha Rh4AG332600 Rh4BG340400 Rh4CG355600 Rh4DG335800
rosa_wichuraiana Rw4G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
Acc36I ACCTGC 2 cut(s) 26, 765
AccB1I GGYRCC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 828
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 1 cut(s) 1163
AclWI GGATC 2 cut(s) 295, 880
AcsI RAATTY 2 cut(s) 79, 1183
AcuI CTGAAG 2 cut(s) 500, 752
AfiI CCNNNNNNNGG 2 cut(s) 861, 895
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 7 cut(s) 403, 440, 464, 677, 746, 912, 1030
AjiI CACGTC 1 cut(s) 128
AjnI CCWGG 3 cut(s) 385, 496, 854
AjuI GAANNNNNNNTTGG 2 cut(s) 1002, 1034
AluBI AGCT 4 cut(s) 337, 503, 526, 760
AluI AGCT 4 cut(s) 337, 503, 526, 760
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 3 cut(s) 118, 636, 925
AlwI GGATC 2 cut(s) 295, 880
AlwNI CAGNNNCTG 1 cut(s) 800
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 1 cut(s) 425
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 2 cut(s) 79, 1183
AspS9I GGNCC 2 cut(s) 425, 1142
AsuHPI GGTGA 2 cut(s) 203, 545
AvaII GGWCC 1 cut(s) 1142
BanI GGYRCC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 505
BbsI GAAGAC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 616
BccI CCATC 5 cut(s) 79, 406, 524, 542, 1131
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BciT130I CCWGG 3 cut(s) 387, 498, 856
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 3 cut(s) 118, 636, 925
BfaI CTAG 2 cut(s) 311, 927
BfmI CTRYAG 2 cut(s) 772, 781
BfuAI ACCTGC 2 cut(s) 26, 765
BglII AGATCT 2 cut(s) 319, 914
BisI GCNGC 1 cut(s) 630
BlsI GCNGC 1 cut(s) 631
Bme1390I CCNGG 3 cut(s) 387, 498, 856
Bme18I GGWCC 1 cut(s) 1142
BmgBI CACGTC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 425, 1142
BmiI GGNNCC 5 cut(s) 170, 179, 495, 616, 1143
BmrFI CCNGG 3 cut(s) 387, 498, 856
BmsI GCATC 3 cut(s) 13, 637, 787
BpiI GAAGAC 1 cut(s) 547
BplI GAGNNNNNCTC 2 cut(s) 815, 847
BpmI CTGGAG 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 173, 986, 1081, 1120
BsaWI WCCGGW 1 cut(s) 259
Bsc4I CCNNNNNNNGG 2 cut(s) 861, 895
Bse1I ACTGG 1 cut(s) 1114
Bse3DI GCAATG 1 cut(s) 1172
BseAI TCCGGA 1 cut(s) 259
BseBI CCWGG 3 cut(s) 387, 498, 856
BseDI CCNNGG 4 cut(s) 173, 986, 1081, 1120
BseGI GGATG 2 cut(s) 553, 855
BseLI CCNNNNNNNGG 2 cut(s) 861, 895
BseMI GCAATG 1 cut(s) 1172
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 1 cut(s) 1114
BseXI GCAGC 1 cut(s) 616
BseYI CCCAGC 1 cut(s) 282
BsgI GTGCAG 1 cut(s) 1143
BshFI GGCC 1 cut(s) 427
BshNI GGYRCC 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 2 cut(s) 1069, 1155
BslI CCNNNNNNNGG 2 cut(s) 861, 895
BsmAI GTCTC 3 cut(s) 118, 636, 925
BsmBI CGTCTC 2 cut(s) 118, 636
BsmFI GGGAC 2 cut(s) 1069, 1155
BsnI GGCC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 505
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
Bsp19I CCATGG 1 cut(s) 986
BspACI CCGC 1 cut(s) 1163
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 1 cut(s) 434
BspEI TCCGGA 1 cut(s) 259
BspHI TCATGA 1 cut(s) 1096
BspLI GGNNCC 5 cut(s) 170, 179, 495, 616, 1143
BspMAI CTGCAG 1 cut(s) 776
BspMI ACCTGC 2 cut(s) 26, 765
BspPI GGATC 2 cut(s) 295, 880
BspT107I GGYRCC 1 cut(s) 614
BsrDI GCAATG 1 cut(s) 1172
BsrI ACTGG 1 cut(s) 1114
BssECI CCNNGG 4 cut(s) 173, 986, 1081, 1120
BssMI GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
BssNAI GTATAC 1 cut(s) 829
BssT1I CCWWGG 1 cut(s) 986
Bst1107I GTATAC 1 cut(s) 829
Bst2UI CCWGG 3 cut(s) 387, 498, 856
Bst4CI ACNGT 1 cut(s) 446
Bst6I CTCTTC 2 cut(s) 854, 928
BstC8I GCNNGC 2 cut(s) 483, 728
BstDEI CTNAG 1 cut(s) 421
BstDSI CCRYGG 3 cut(s) 173, 986, 1120
BstENI CCTNNNNNAGG 1 cut(s) 859
BstF5I GGATG 2 cut(s) 553, 855
BstKTI GATC 8 cut(s) 290, 322, 382, 883, 888, 917, 955, 976
BstMAI GTCTC 3 cut(s) 118, 636, 925
BstMBI GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
BstMWI GCNNNNNNNGC 4 cut(s) 32, 662, 709, 1077
BstNI CCWGG 3 cut(s) 387, 498, 856
BstSCI CCNGG 3 cut(s) 385, 496, 854
BstSFI CTRYAG 2 cut(s) 772, 781
BstV1I GCAGC 1 cut(s) 616
BstV2I GAAGAC 1 cut(s) 547
BstX2I RGATCY 3 cut(s) 319, 885, 914
BstXI CCANNNNNNTGG 1 cut(s) 1121
BstYI RGATCY 3 cut(s) 319, 885, 914
BstZ17I GTATAC 1 cut(s) 829
BsuRI GGCC 1 cut(s) 427
BtgI CCRYGG 3 cut(s) 173, 986, 1120
BtrI CACGTC 1 cut(s) 128
BtsCI GGATG 2 cut(s) 553, 855
BveI ACCTGC 2 cut(s) 26, 765
Cac8I GCNNGC 2 cut(s) 483, 728
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 1 cut(s) 1096
Cfr13I GGNCC 2 cut(s) 425, 1142
CseI GACGC 1 cut(s) 487
CviAII CATG 8 cut(s) 164, 454, 510, 689, 713, 977, 987, 1097
CviJI RGCY 9 cut(s) 178, 327, 337, 427, 503, 514, 526, 760, 1080
CviKI_1 RGCY 9 cut(s) 178, 327, 337, 427, 503, 514, 526, 760, 1080
DdeI CTNAG 1 cut(s) 421
DpnI GATC 8 cut(s) 289, 321, 381, 882, 887, 916, 954, 975
DpnII GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
Eam1104I CTCTTC 2 cut(s) 854, 928
EarI CTCTTC 2 cut(s) 854, 928
Ecl136II GAGCTC 1 cut(s) 503
Eco130I CCWWGG 1 cut(s) 986
Eco24I GRGCYC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 1142
Eco53kI GAGCTC 1 cut(s) 503
Eco57I CTGAAG 2 cut(s) 500, 752
EcoICRI GAGCTC 1 cut(s) 503
EcoNI CCTNNNNNAGG 1 cut(s) 859
EcoO109I RGGNCCY 2 cut(s) 425, 1142
EcoRII CCWGG 3 cut(s) 385, 496, 854
EcoT14I CCWWGG 1 cut(s) 986
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 986
Esp3I CGTCTC 2 cut(s) 118, 636
FaeI CATG 8 cut(s) 167, 457, 513, 692, 716, 980, 990, 1100
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 2 cut(s) 1069, 1155
FatI CATG 8 cut(s) 163, 453, 509, 688, 712, 976, 986, 1096
FauNDI CATATG 1 cut(s) 599
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 828
Fnu4HI GCNGC 1 cut(s) 630
FokI GGATG 2 cut(s) 560, 862
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 1 cut(s) 630
FspBI CTAG 2 cut(s) 311, 927
GluI GCNGC 1 cut(s) 630
GsaI CCCAGC 1 cut(s) 286
GsuI CTGGAG 1 cut(s) 408
HaeIII GGCC 1 cut(s) 427
HapII CCGG 1 cut(s) 260
HgaI GACGC 1 cut(s) 487
Hin1II CATG 8 cut(s) 167, 457, 513, 692, 716, 980, 990, 1100
HindIII AAGCTT 2 cut(s) 335, 524
HinfI GANTC 8 cut(s) 307, 403, 460, 470, 746, 1004, 1030, 1093
HpaII CCGG 1 cut(s) 260
HphI GGTGA 2 cut(s) 203, 545
Hpy166II GTNNAC 4 cut(s) 536, 829, 932, 1155
Hpy188I TCNGA 4 cut(s) 151, 369, 885, 1009
Hpy188III TCNNGA 8 cut(s) 56, 260, 296, 437, 912, 1043, 1097, 1130
Hpy8I GTNNAC 4 cut(s) 536, 829, 932, 1155
HpyCH4III ACNGT 1 cut(s) 446
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 6 cut(s) 114, 137, 232, 632, 774, 1160
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 662, 709, 1077
HpyF3I CTNAG 1 cut(s) 421
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 8 cut(s) 167, 457, 513, 692, 716, 980, 990, 1100
Kpn2I TCCGGA 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
LmnI GCTCC 3 cut(s) 183, 500, 661
Lsp1109I GCAGC 1 cut(s) 616
LweI GCATC 3 cut(s) 13, 637, 787
MaeI CTAG 2 cut(s) 311, 927
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 5 cut(s) 467, 694, 808, 946, 1130
MalI GATC 8 cut(s) 289, 321, 381, 882, 887, 916, 954, 975
MboI GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
MboII GAAGA 6 cut(s) 22, 374, 442, 547, 871, 915
MflI RGATCY 3 cut(s) 319, 885, 914
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 4 cut(s) 79, 606, 633, 1183
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 8 cut(s) 52, 256, 438, 442, 661, 817, 855, 1091
MroI TCCGGA 1 cut(s) 259
MseI TTAA 4 cut(s) 354, 609, 636, 942
MslI CAYNNNNRTG 3 cut(s) 21, 687, 1165
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 3 cut(s) 387, 498, 856
MvaI CCWGG 3 cut(s) 387, 498, 856
MwoI GCNNNNNNNGC 4 cut(s) 32, 662, 709, 1077
NcoI CCATGG 1 cut(s) 986
NdeI CATATG 1 cut(s) 599
NdeII GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
NlaIII CATG 8 cut(s) 167, 457, 513, 692, 716, 980, 990, 1100
NlaIV GGNNCC 5 cut(s) 170, 179, 495, 616, 1143
NmeAIII GCCGAG 1 cut(s) 1062
NmuCI GTSAC 3 cut(s) 467, 946, 1130
PagI TCATGA 1 cut(s) 1096
PfeI GAWTC 7 cut(s) 307, 403, 460, 746, 1004, 1030, 1093
PfoI TCCNGGA 3 cut(s) 385, 496, 854
PkrI GCNGC 1 cut(s) 631
PleI GAGTC 1 cut(s) 464
PpsI GAGTC 1 cut(s) 464
PpuMI RGGWCCY 1 cut(s) 1142
PsiI TTATAA 1 cut(s) 594
Psp124BI GAGCTC 1 cut(s) 505
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 3 cut(s) 385, 496, 854
PspFI CCCAGC 1 cut(s) 282
PspGI CCWGG 3 cut(s) 385, 496, 854
PspN4I GGNNCC 5 cut(s) 170, 179, 495, 616, 1143
PspPI GGNCC 2 cut(s) 425, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PstI CTGCAG 1 cut(s) 776
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 3 cut(s) 319, 885, 914
RseI CAYNNNNRTG 3 cut(s) 21, 687, 1165
SacI GAGCTC 1 cut(s) 505
SaqAI TTAA 4 cut(s) 354, 609, 636, 942
SatI GCNGC 1 cut(s) 630
Sau3AI GATC 8 cut(s) 287, 319, 379, 880, 885, 914, 952, 973
Sau96I GGNCC 2 cut(s) 425, 1142
SchI GAGTC 1 cut(s) 464
ScrFI CCNGG 3 cut(s) 387, 498, 856
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 3 cut(s) 13, 637, 787
SfcI CTRYAG 2 cut(s) 772, 781
SinI GGWCC 1 cut(s) 1142
SmiMI CAYNNNNRTG 3 cut(s) 21, 687, 1165
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
Sse9I AATT 4 cut(s) 79, 606, 633, 1183
SsiI CCGC 1 cut(s) 1163
SspMI CTAG 2 cut(s) 311, 927
SstI GAGCTC 1 cut(s) 505
StyD4I CCNGG 3 cut(s) 385, 496, 854
StyI CCWWGG 1 cut(s) 986
TaaI ACNGT 1 cut(s) 446
TaiI ACGT 1 cut(s) 130
TasI AATT 4 cut(s) 79, 606, 633, 1183
TfiI GAWTC 7 cut(s) 307, 403, 460, 746, 1004, 1030, 1093
Tru1I TTAA 4 cut(s) 354, 609, 636, 942
Tru9I TTAA 4 cut(s) 354, 609, 636, 942
TseFI GTSAC 3 cut(s) 467, 946, 1130
TseI GCWGC 1 cut(s) 629
Tsp45I GTSAC 3 cut(s) 467, 946, 1130
TspDTI ATGAA 6 cut(s) 17, 442, 659, 705, 729, 1113
TspGWI ACGGA 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 1142
XagI CCTNNNNNAGG 1 cut(s) 859
XapI RAATTY 2 cut(s) 79, 1183
XmiI GTMKAC 1 cut(s) 828
XspI CTAG 2 cut(s) 311, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.