Rroxscaffold_5G00375830

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014698
Physical Location & Seq
Reverse (-)
56562478 .. 56565039
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375830.1

Sequence Viewer

Length: 1227 bp
ATGGGGCAAAGAAGGAGAAATAAAAAGCAGAAGAAGCCTATAGATATTGATTGGGCAGGTCTGCCTGACAATATTCTTGAGATAATCTTTGAGCGACTGAATTTGATGGATTGCATGTCTGTTAGTGAGGTTTGCAAATCTTGGAGACATGTTGTTGCACAAGAACTTTCTGGTTGGCAAGGGCATGGGTTTCCATGGCTCTTGATGTCTGGTGAGAAAGACAAAAGGGTAAGGACTTGTGTTAGCATACTACAGGAGCAAGAGTGGGAGATGTTGCTTCCGAAGGCTTATGGTAGATACTGCTGGGGATCATACCATGACTGGTTGATTCTAGTAAAAGATCTTGGCTCTTTCAGGCTTGATATTAGCTTGTTTAACCCATTCACTCGGAAAGAAATTGATCTTCCCGGAGCATGGAACTTTTATCACAAGATGGTTCTCTCAAGGCTTCCCTCTCTTGAAAACTTTGTCTGCATGCTGGTTCATAGTCACTCCCAGAAGCTTGCCTTTTGGGTCCCAGGAGCTCAATCATGGCTTGAGCAGAAGCTAGATGGTGAACCATTTGTGGATGCTGTCTTCTCCAATGGAAGTTTCTATCTCATTGGCAGTGATTATAACATTTGGGAAATTAAGGGTGCTGATATTTTTGCTGCCATTAACAGAGTCAATGCATCTGCCCCTGATGCTTTTGATGTCGAAAGGCATTTCCATGAAGTAACTATGTCAGATGAGCATGAAAATGAAAATAATGGTGTGCTGAAGTATCTTGTTGAATCTTGTGGGGAGCTTTTACTTGTTTGTAGGTTCTATAGTACTAACCCAGGTGCTGTGCTGGTAACAAATTATTTTAAGGTATATTCACTGGATTTTAGCCAAATGTCCTGGAAGAGGGTAGAGAGTTTGGGAGATCGAATACTATTTCTGGGCAAGTGTTGTTCGAGATCTGTCTCTTCCAGTGGACTTGGTCTTAACATGACTGATTGTATCTATTTCTCTAATGATCATGCTGTTCCGTGGTGGAACGAATGGGATTCTGATCATCTTGATGGCATTTCAACTCGTCTTGGTCTGGACAATAGTGGGAGAAAGGATTGGGGCTTTTTCAGCCTCAGCAACAAGAATCATGAAACTTTGTCTATTCGGTTCCGTGGCAATCGAGACAGATGGGGACCTGTTTGGTTTACTGCGCCACAGTGGTGGTGTTGTAAGAATTTGGTTCTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

408

Amino Acids

47.27

Weight (kDa)

6.19

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 18 - 59 2.2e-07 F-box-like
F-box PF00646 19 - 53 2.5e-08 F-box domain
Beta-prop_KIB1-4 PF03478 91 - 344 3.8e-47 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27200
malus_domestica MD13G1035900.v1.1
prunus_persica Prupe.6G174300_v2.0.a1 Prupe.6G178100_v2.0.a1
pyrus_communis pycom13g03130 pycom16g03310
rosa_chinensis RchiOBHm_Chr4g0434481
rosa_laevigata RLG00000006668
rosa_multiflora Rmu_sc0002251.1_g000016 Rmu_sc0005899.1_g000009
rosa_roxburghii Rroxscaffold_5G00375830
rosa_rugosa Rorug04G0277000
rosa_samantha Rh4AG332600 Rh4BG340400 Rh4CG355600 Rh4DG335800
rosa_wichuraiana Rw4G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
Acc36I ACCTGC 1 cut(s) 47
AclWI GGATC 1 cut(s) 316
AcsI RAATTY 2 cut(s) 100, 1210
AcuI CTGAAG 1 cut(s) 779
AfaI GTAC 1 cut(s) 814
AfiI CCNNNNNNNGG 2 cut(s) 414, 888
AflIII ACRYGT 1 cut(s) 148
AgsI TTSAA 3 cut(s) 461, 773, 1056
AjnI CCWGG 3 cut(s) 517, 820, 881
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AleI CACNNNNGTG 1 cut(s) 1195
AluBI AGCT 5 cut(s) 369, 502, 524, 547, 787
AluI AGCT 5 cut(s) 369, 502, 524, 547, 787
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 3 cut(s) 139, 952, 1152
AlwI GGATC 1 cut(s) 316
AlwNI CAGNNNCTG 1 cut(s) 827
ApeKI GCWGC 1 cut(s) 650
ApoI RAATTY 2 cut(s) 100, 1210
AspLEI GCGC 1 cut(s) 1189
AspS9I GGNCC 2 cut(s) 514, 1169
AsuC2I CCSGG 1 cut(s) 408
AsuHPI GGTGA 2 cut(s) 224, 566
AvaII GGWCC 2 cut(s) 514, 1169
BanII GRGCYC 1 cut(s) 526
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 1 cut(s) 526
BbvCI CCTCAGC 1 cut(s) 1109
BbvI GCAGC 1 cut(s) 637
BccI CCATC 5 cut(s) 100, 427, 545, 1040, 1158
BciT130I CCWGG 3 cut(s) 519, 822, 883
BclI TGATCA 2 cut(s) 1000, 1036
BcnI CCSGG 1 cut(s) 408
BcoDI GTCTC 3 cut(s) 139, 952, 1152
BfaI CTAG 3 cut(s) 332, 548, 1225
BfmI CTRYAG 3 cut(s) 39, 251, 808
BfuAI ACCTGC 1 cut(s) 47
BglII AGATCT 2 cut(s) 340, 941
BisI GCNGC 1 cut(s) 651
BlsI GCNGC 1 cut(s) 652
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 4 cut(s) 408, 519, 822, 883
Bme18I GGWCC 2 cut(s) 514, 1169
BmgT120I GGNCC 2 cut(s) 514, 1169
BmiI GGNNCC 4 cut(s) 515, 516, 1145, 1170
BmrFI CCNGG 4 cut(s) 408, 519, 822, 883
BmsI GCATC 3 cut(s) 559, 673, 680
BpiI GAAGAC 1 cut(s) 568
Bpu10I CCTNAGC 1 cut(s) 1109
BpuEI CTTGAG 3 cut(s) 98, 427, 557
BpuMI CCSGG 1 cut(s) 408
BsaBI GATNNNNATC 1 cut(s) 1035
BsaJI CCNNGG 5 cut(s) 194, 517, 820, 1013, 1147
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 414, 888
Bse1I ACTGG 3 cut(s) 326, 867, 954
Bse8I GATNNNNATC 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 519, 822, 883
BseDI CCNNGG 5 cut(s) 194, 517, 820, 1013, 1147
BseGI GGATG 1 cut(s) 574
BseJI GATNNNNATC 1 cut(s) 1035
BseLI CCNNNNNNNGG 2 cut(s) 414, 888
BseMII CTCAG 1 cut(s) 1123
BseNI ACTGG 3 cut(s) 326, 867, 954
BseXI GCAGC 1 cut(s) 637
BseYI CCCAGC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 2 cut(s) 500, 1182
BslI CCNNNNNNNGG 2 cut(s) 414, 888
BsmAI GTCTC 3 cut(s) 139, 952, 1152
BsmFI GGGAC 2 cut(s) 500, 1182
Bsp1286I GDGCHC 1 cut(s) 526
Bsp143I GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
Bsp19I CCATGG 1 cut(s) 194
BspCNI CTCAG 1 cut(s) 1122
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 4 cut(s) 515, 516, 1145, 1170
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 1 cut(s) 316
BsrI ACTGG 3 cut(s) 326, 867, 954
BssECI CCNNGG 5 cut(s) 194, 517, 820, 1013, 1147
BssMI GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
BssT1I CCWWGG 1 cut(s) 194
Bst2UI CCWGG 3 cut(s) 519, 822, 883
Bst4CI ACNGT 1 cut(s) 1194
Bst6I CTCTTC 2 cut(s) 881, 955
BstC8I GCNNGC 2 cut(s) 476, 504
BstDEI CTNAG 1 cut(s) 1109
BstDSI CCRYGG 3 cut(s) 194, 1013, 1147
BstENI CCTNNNNNAGG 1 cut(s) 886
BstF5I GGATG 1 cut(s) 574
BstHHI GCGC 1 cut(s) 1189
BstKTI GATC 7 cut(s) 311, 343, 403, 910, 944, 1003, 1039
BstMAI GTCTC 3 cut(s) 139, 952, 1152
BstMBI GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
BstMWI GCNNNNNNNGC 3 cut(s) 34, 683, 1104
BstNI CCWGG 3 cut(s) 519, 822, 883
BstNSI RCATGY 3 cut(s) 118, 152, 478
BstSCI CCNGG 4 cut(s) 406, 517, 820, 881
BstSFI CTRYAG 3 cut(s) 39, 251, 808
BstV1I GCAGC 1 cut(s) 637
BstV2I GAAGAC 1 cut(s) 568
BstX2I RGATCY 2 cut(s) 340, 941
BstXI CCANNNNNNTGG 1 cut(s) 1197
BstYI RGATCY 2 cut(s) 340, 941
BtgI CCRYGG 3 cut(s) 194, 1013, 1147
BtsCI GGATG 1 cut(s) 574
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 4 cut(s) 613, 860, 961, 1199
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 2 cut(s) 476, 504
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 1 cut(s) 1189
Cfr13I GGNCC 2 cut(s) 514, 1169
Csp6I GTAC 1 cut(s) 813
CviQI GTAC 1 cut(s) 813
DdeI CTNAG 1 cut(s) 1109
DpnI GATC 7 cut(s) 310, 342, 402, 909, 943, 1002, 1038
DpnII GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
Eam1104I CTCTTC 2 cut(s) 881, 955
EarI CTCTTC 2 cut(s) 881, 955
Ecl136II GAGCTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 526
Eco47I GGWCC 2 cut(s) 514, 1169
Eco53kI GAGCTC 1 cut(s) 524
Eco57I CTGAAG 1 cut(s) 779
EcoICRI GAGCTC 1 cut(s) 524
EcoNI CCTNNNNNAGG 1 cut(s) 886
EcoO109I RGGNCCY 2 cut(s) 514, 1169
EcoRII CCWGG 3 cut(s) 517, 820, 881
EcoT14I CCWWGG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 673
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 1 cut(s) 194
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 500, 1182
FbaI TGATCA 2 cut(s) 1000, 1036
Fnu4HI GCNGC 1 cut(s) 651
FokI GGATG 1 cut(s) 581
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 1 cut(s) 651
FspBI CTAG 3 cut(s) 332, 548, 1225
GlaI GCGC 1 cut(s) 1188
GluI GCNGC 1 cut(s) 651
GsaI CCCAGC 1 cut(s) 307
HapII CCGG 1 cut(s) 408
HhaI GCGC 1 cut(s) 1189
Hin6I GCGC 1 cut(s) 1187
HinP1I GCGC 1 cut(s) 1187
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 5 cut(s) 328, 663, 773, 1031, 1120
HpaII CCGG 1 cut(s) 408
HphI GGTGA 2 cut(s) 224, 566
Hpy166II GTNNAC 3 cut(s) 557, 959, 1182
Hpy188I TCNGA 4 cut(s) 282, 390, 727, 1036
Hpy188III TCNNGA 8 cut(s) 77, 202, 458, 939, 1043, 1070, 1124, 1157
Hpy8I GTNNAC 3 cut(s) 557, 959, 1182
HpyAV CCTTC 2 cut(s) 6, 277
HpyCH4III ACNGT 1 cut(s) 1194
HpyCH4V TGCA 5 cut(s) 114, 135, 158, 474, 671
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 683, 1104
HpyF3I CTNAG 1 cut(s) 1109
HspAI GCGC 1 cut(s) 1187
KflI GGGWCCC 1 cut(s) 514
Ksp22I TGATCA 2 cut(s) 1000, 1036
Kzo9I GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
LmnI GCTCC 4 cut(s) 256, 410, 521, 784
Lsp1109I GCAGC 1 cut(s) 637
LweI GCATC 3 cut(s) 559, 673, 680
MaeI CTAG 3 cut(s) 332, 548, 1225
MaeIII GTNAC 3 cut(s) 488, 715, 835
MalI GATC 7 cut(s) 310, 342, 402, 909, 943, 1002, 1038
MboI GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
MboII GAAGA 5 cut(s) 43, 395, 568, 898, 942
MflI RGATCY 2 cut(s) 340, 941
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 5 cut(s) 100, 396, 627, 841, 1210
MlyI GAGTC 1 cut(s) 672
MnlI CCTC 4 cut(s) 121, 463, 882, 1118
Mph1103I ATGCAT 1 cut(s) 673
MseI TTAA 5 cut(s) 375, 630, 657, 849, 969
MslI CAYNNNNRTG 4 cut(s) 708, 738, 1044, 1195
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 4 cut(s) 408, 519, 822, 883
MvaI CCWGG 3 cut(s) 519, 822, 883
MwoI GCNNNNNNNGC 3 cut(s) 34, 683, 1104
NciI CCSGG 1 cut(s) 408
NcoI CCATGG 1 cut(s) 194
NdeII GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
NlaIV GGNNCC 4 cut(s) 515, 516, 1145, 1170
NmuCI GTSAC 1 cut(s) 488
NsiI ATGCAT 1 cut(s) 673
NspI RCATGY 3 cut(s) 118, 152, 478
OliI CACNNNNGTG 1 cut(s) 1195
PaeI GCATGC 1 cut(s) 478
PagI TCATGA 1 cut(s) 1123
PciI ACATGT 1 cut(s) 148
PfeI GAWTC 4 cut(s) 328, 773, 1031, 1120
PflFI GACNNNGTC 1 cut(s) 963
PfoI TCCNGGA 2 cut(s) 406, 881
PkrI GCNGC 1 cut(s) 652
PleI GAGTC 1 cut(s) 671
PpsI GAGTC 1 cut(s) 671
PpuMI RGGWCCY 2 cut(s) 514, 1169
PscI ACATGT 1 cut(s) 148
PsiI TTATAA 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 526
Psp5II RGGWCCY 2 cut(s) 514, 1169
Psp6I CCWGG 3 cut(s) 517, 820, 881
PspFI CCCAGC 1 cut(s) 303
PspGI CCWGG 3 cut(s) 517, 820, 881
PspN4I GGNNCC 4 cut(s) 515, 516, 1145, 1170
PspPI GGNCC 2 cut(s) 514, 1169
PspPPI RGGWCCY 2 cut(s) 514, 1169
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 2 cut(s) 340, 941
PsyI GACNNNGTC 1 cut(s) 963
RsaI GTAC 1 cut(s) 814
RsaNI GTAC 1 cut(s) 813
RseI CAYNNNNRTG 4 cut(s) 708, 738, 1044, 1195
SacI GAGCTC 1 cut(s) 526
SaqAI TTAA 5 cut(s) 375, 630, 657, 849, 969
SatI GCNGC 1 cut(s) 651
Sau3AI GATC 7 cut(s) 308, 340, 400, 907, 941, 1000, 1036
Sau96I GGNCC 2 cut(s) 514, 1169
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 1 cut(s) 672
ScrFI CCNGG 4 cut(s) 408, 519, 822, 883
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 3 cut(s) 559, 673, 680
SfcI CTRYAG 3 cut(s) 39, 251, 808
SinI GGWCC 2 cut(s) 514, 1169
SmiMI CAYNNNNRTG 4 cut(s) 708, 738, 1044, 1195
SmlI CTYRAG 3 cut(s) 77, 442, 536
SmoI CTYRAG 3 cut(s) 77, 442, 536
SphI GCATGC 1 cut(s) 478
Sse9I AATT 5 cut(s) 100, 396, 627, 841, 1210
SspI AATATT 1 cut(s) 73
SspMI CTAG 3 cut(s) 332, 548, 1225
SstI GAGCTC 1 cut(s) 526
StyD4I CCNGG 4 cut(s) 406, 517, 820, 881
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 1194
TaqI TCGA 4 cut(s) 696, 910, 938, 1156
TasI AATT 5 cut(s) 100, 396, 627, 841, 1210
TatI WGTACW 1 cut(s) 812
TfiI GAWTC 4 cut(s) 328, 773, 1031, 1120
Tru1I TTAA 5 cut(s) 375, 630, 657, 849, 969
Tru9I TTAA 5 cut(s) 375, 630, 657, 849, 969
TscAI CASTG 4 cut(s) 613, 867, 961, 1199
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 1 cut(s) 650
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 5 cut(s) 473, 726, 750, 756, 1140
TspGWI ACGGA 2 cut(s) 1002, 1136
TspRI CASTG 4 cut(s) 613, 867, 961, 1199
Tth111I GACNNNGTC 1 cut(s) 963
VpaK11BI GGWCC 2 cut(s) 514, 1169
XagI CCTNNNNNAGG 1 cut(s) 886
XapI RAATTY 2 cut(s) 100, 1210
XceI RCATGY 3 cut(s) 118, 152, 478
XspI CTAG 3 cut(s) 332, 548, 1225
ZrmI AGTACT 1 cut(s) 814
Zsp2I ATGCAT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.