RLG00000006668

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014698
Physical Location & Seq
Forward (+)
9513994 .. 9515109
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006668

Sequence Viewer

Length: 963 bp
ATGGATTGCATGTCTGTTAGTGAGGTTTGCAAATCTTGGAGACATGTTGTTGCACAAGAACTTTCTGGTTGGCAAGGGCATGGGTTTCCGTGGCTCTTGATGTCTGGTGAGAAAGACAAGAGGGTAAGGACTTGTGTTAGCATACTACAGGAGCAAGAGTGGGAGATGCTGCTTCCGGAGGCTTATGGTAGGTACTGCTGGGGGTCATACCATGACTGGTTGATTCTAGTAAAAGATCTTGGCTCTTTCAGGCTTGATATTAGCTTGTTTAACCCATTCACTCGGAAACAAATTGATCTTCCCGGAGCATGGAACTTGTATCACAAGATGGTTCTCTCAAGGCTTCCCTCTCTTGAAAACTTTATCTGCATGCTGGTTCATAGTCACTCCCAGAAGCTTGCCTTTTGGGTCCCAGGAGCTCAATCATGGCTTGAGCATAAGCTAGATGGTGAACCATTTGTGGATGCTGTCTTCTCCAATGGAAGTTTCTATCTCATTGGCAATGATTATAACATTTGGGAAATAAAGGGTGCTGATATTTTTGCTGCCATTAACAGAGTCAATGCATCTGCCCCTGATGCTTTTGATGTCGAAAGGCATTTCCATGATGTAACTATGTCAGATGAGCATGAAAATAATGGTGTGCTGAAGTATCTTGTTGAATCTTGTGGGGAGCTTCTACTTGTTTGTAGTGGACTTGGTCTTAACATGACTGATTGTATCTATTTCTCTAATGATCATGCTGTTCCGTGGTGGAACGAATGGGATTCTGATCATCTTGATGGCATTTCAACTCGTCTTGGTCTGGACAATAGTGGGAGAAAGGATTGGGGCTTTTTCAGCCTCAGCAACAAAAATCATGAAACTTTGTCTATTCGGTTCCGTGGCAATCGAGATAGATGGGGACCTGTTTGGTTTACTGCGCCACAGTGGTGGTGTTGTAAGAATTTGGTTCTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

321

Amino Acids

37.0

Weight (kDa)

5.5

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 56 - 231 2.3e-28 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27200
malus_domestica MD13G1035900.v1.1
prunus_persica Prupe.6G174300_v2.0.a1 Prupe.6G178100_v2.0.a1
pyrus_communis pycom13g03130 pycom16g03310
rosa_chinensis RchiOBHm_Chr4g0434481
rosa_laevigata RLG00000006668
rosa_multiflora Rmu_sc0002251.1_g000016 Rmu_sc0005899.1_g000009
rosa_roxburghii Rroxscaffold_5G00375830
rosa_rugosa Rorug04G0277000
rosa_samantha Rh4AG332600 Rh4BG340400 Rh4CG355600 Rh4DG335800
rosa_wichuraiana Rw4G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 175
AcsI RAATTY 1 cut(s) 946
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 309
AflIII ACRYGT 1 cut(s) 43
AgsI TTSAA 3 cut(s) 356, 662, 792
AjnI CCWGG 1 cut(s) 412
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AleI CACNNNNGTG 1 cut(s) 931
AluBI AGCT 5 cut(s) 264, 397, 419, 442, 676
AluI AGCT 5 cut(s) 264, 397, 419, 442, 676
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 169, 545
ApoI RAATTY 1 cut(s) 946
AspLEI GCGC 1 cut(s) 925
AspS9I GGNCC 2 cut(s) 409, 905
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 119, 461
AvaII GGWCC 2 cut(s) 409, 905
BanII GRGCYC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 421
BbvCI CCTCAGC 1 cut(s) 845
BbvI GCAGC 2 cut(s) 156, 532
BccI CCATC 4 cut(s) 322, 440, 776, 894
BciT130I CCWGG 1 cut(s) 414
BclI TGATCA 2 cut(s) 736, 772
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 3 cut(s) 227, 443, 961
BfmI CTRYAG 1 cut(s) 146
BglII AGATCT 1 cut(s) 235
BisI GCNGC 2 cut(s) 170, 546
BlsI GCNGC 2 cut(s) 171, 547
Bme1390I CCNGG 2 cut(s) 303, 414
Bme18I GGWCC 2 cut(s) 409, 905
BmgT120I GGNCC 2 cut(s) 409, 905
BmiI GGNNCC 4 cut(s) 410, 411, 881, 906
BmrFI CCNGG 2 cut(s) 303, 414
BmsI GCATC 4 cut(s) 156, 454, 568, 575
BpiI GAAGAC 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 845
BpuEI CTTGAG 2 cut(s) 322, 452
BpuMI CCSGG 1 cut(s) 303
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 4 cut(s) 89, 412, 749, 883
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 309
Bse1I ACTGG 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 771
BseAI TCCGGA 1 cut(s) 175
BseBI CCWGG 1 cut(s) 414
BseDI CCNNGG 4 cut(s) 89, 412, 749, 883
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 1 cut(s) 309
BseMI GCAATG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 859
BseNI ACTGG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 156, 532
BseYI CCCAGC 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 2 cut(s) 176, 303
BslFI GGGAC 2 cut(s) 395, 918
BslI CCNNNNNNNGG 1 cut(s) 309
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 2 cut(s) 395, 918
Bsp1286I GDGCHC 1 cut(s) 421
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 4 cut(s) 235, 295, 736, 772
BspCNI CTCAG 1 cut(s) 858
BspEI TCCGGA 1 cut(s) 175
BspHI TCATGA 1 cut(s) 859
BspLI GGNNCC 4 cut(s) 410, 411, 881, 906
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 4 cut(s) 89, 412, 749, 883
BssMI GATC 4 cut(s) 235, 295, 736, 772
Bst2UI CCWGG 1 cut(s) 414
Bst4CI ACNGT 1 cut(s) 930
BstC8I GCNNGC 2 cut(s) 371, 399
BstDEI CTNAG 1 cut(s) 845
BstDSI CCRYGG 3 cut(s) 89, 749, 883
BstF5I GGATG 1 cut(s) 469
BstHHI GCGC 1 cut(s) 925
BstKTI GATC 4 cut(s) 238, 298, 739, 775
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 4 cut(s) 235, 295, 736, 772
BstMWI GCNNNNNNNGC 2 cut(s) 578, 840
BstNI CCWGG 1 cut(s) 414
BstNSI RCATGY 3 cut(s) 13, 47, 373
BstSCI CCNGG 2 cut(s) 301, 412
BstSFI CTRYAG 1 cut(s) 146
BstV1I GCAGC 2 cut(s) 156, 532
BstV2I GAAGAC 1 cut(s) 463
BstX2I RGATCY 1 cut(s) 235
BstXI CCANNNNNNTGG 1 cut(s) 933
BstYI RGATCY 1 cut(s) 235
BtgI CCRYGG 3 cut(s) 89, 749, 883
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 935
Cac8I GCNNGC 2 cut(s) 371, 399
CciI TCATGA 1 cut(s) 859
CfoI GCGC 1 cut(s) 925
Cfr13I GGNCC 2 cut(s) 409, 905
Csp6I GTAC 1 cut(s) 193
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 1 cut(s) 845
DpnI GATC 4 cut(s) 237, 297, 738, 774
DpnII GATC 4 cut(s) 235, 295, 736, 772
Ecl136II GAGCTC 1 cut(s) 419
Eco24I GRGCYC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 409, 905
Eco53kI GAGCTC 1 cut(s) 419
Eco57I CTGAAG 1 cut(s) 668
EcoICRI GAGCTC 1 cut(s) 419
EcoO109I RGGNCCY 2 cut(s) 409, 905
EcoRII CCWGG 1 cut(s) 412
EcoT22I ATGCAT 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 421
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FaqI GGGAC 2 cut(s) 395, 918
FbaI TGATCA 2 cut(s) 736, 772
Fnu4HI GCNGC 2 cut(s) 170, 546
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 170, 546
FspBI CTAG 3 cut(s) 227, 443, 961
GlaI GCGC 1 cut(s) 924
GluI GCNGC 2 cut(s) 170, 546
GsaI CCCAGC 1 cut(s) 202
HapII CCGG 2 cut(s) 176, 303
HhaI GCGC 1 cut(s) 925
Hin6I GCGC 1 cut(s) 923
HinP1I GCGC 1 cut(s) 923
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 4 cut(s) 223, 558, 662, 767
HpaII CCGG 2 cut(s) 176, 303
HphI GGTGA 2 cut(s) 119, 461
Hpy166II GTNNAC 3 cut(s) 452, 695, 918
Hpy188I TCNGA 3 cut(s) 285, 622, 772
Hpy188III TCNNGA 7 cut(s) 97, 176, 353, 779, 806, 860, 893
Hpy8I GTNNAC 3 cut(s) 452, 695, 918
HpyCH4III ACNGT 1 cut(s) 930
HpyCH4V TGCA 5 cut(s) 9, 30, 53, 369, 566
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 840
HpyF3I CTNAG 1 cut(s) 845
HspAI GCGC 1 cut(s) 923
KflI GGGWCCC 1 cut(s) 409
Kpn2I TCCGGA 1 cut(s) 175
Ksp22I TGATCA 2 cut(s) 736, 772
Kzo9I GATC 4 cut(s) 235, 295, 736, 772
LmnI GCTCC 4 cut(s) 151, 305, 416, 673
Lsp1109I GCAGC 2 cut(s) 156, 532
LweI GCATC 4 cut(s) 156, 454, 568, 575
MaeI CTAG 3 cut(s) 227, 443, 961
MaeIII GTNAC 2 cut(s) 383, 610
MalI GATC 4 cut(s) 237, 297, 738, 774
MboI GATC 4 cut(s) 235, 295, 736, 772
MboII GAAGA 2 cut(s) 290, 463
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 2 cut(s) 291, 946
MlyI GAGTC 1 cut(s) 567
MnlI CCTC 5 cut(s) 16, 114, 172, 358, 854
Mph1103I ATGCAT 1 cut(s) 568
MroI TCCGGA 1 cut(s) 175
MseI TTAA 3 cut(s) 270, 552, 705
MslI CAYNNNNRTG 3 cut(s) 603, 780, 931
MspI CCGG 2 cut(s) 176, 303
MspR9I CCNGG 2 cut(s) 303, 414
MvaI CCWGG 1 cut(s) 414
MwoI GCNNNNNNNGC 2 cut(s) 578, 840
NciI CCSGG 1 cut(s) 303
NdeII GATC 4 cut(s) 235, 295, 736, 772
NlaIV GGNNCC 4 cut(s) 410, 411, 881, 906
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 3 cut(s) 13, 47, 373
OliI CACNNNNGTG 1 cut(s) 931
PaeI GCATGC 1 cut(s) 373
PagI TCATGA 1 cut(s) 859
PciI ACATGT 1 cut(s) 43
PfeI GAWTC 3 cut(s) 223, 662, 767
PflFI GACNNNGTC 1 cut(s) 699
PfoI TCCNGGA 1 cut(s) 301
PkrI GCNGC 2 cut(s) 171, 547
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PpuMI RGGWCCY 2 cut(s) 409, 905
PscI ACATGT 1 cut(s) 43
PsiI TTATAA 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 421
Psp5II RGGWCCY 2 cut(s) 409, 905
Psp6I CCWGG 1 cut(s) 412
PspFI CCCAGC 1 cut(s) 198
PspGI CCWGG 1 cut(s) 412
PspN4I GGNNCC 4 cut(s) 410, 411, 881, 906
PspPI GGNCC 2 cut(s) 409, 905
PspPPI RGGWCCY 2 cut(s) 409, 905
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 699
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 3 cut(s) 603, 780, 931
SacI GAGCTC 1 cut(s) 421
SaqAI TTAA 3 cut(s) 270, 552, 705
SatI GCNGC 2 cut(s) 170, 546
Sau3AI GATC 4 cut(s) 235, 295, 736, 772
Sau96I GGNCC 2 cut(s) 409, 905
SchI GAGTC 1 cut(s) 567
ScrFI CCNGG 2 cut(s) 303, 414
SduI GDGCHC 1 cut(s) 421
SetI ASST 8 cut(s) 27, 194, 266, 399, 421, 444, 678, 910
SfaNI GCATC 4 cut(s) 156, 454, 568, 575
SfcI CTRYAG 1 cut(s) 146
SinI GGWCC 2 cut(s) 409, 905
SmiMI CAYNNNNRTG 3 cut(s) 603, 780, 931
SmlI CTYRAG 2 cut(s) 337, 431
SmoI CTYRAG 2 cut(s) 337, 431
SphI GCATGC 1 cut(s) 373
Sse9I AATT 2 cut(s) 291, 946
SspMI CTAG 3 cut(s) 227, 443, 961
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 2 cut(s) 301, 412
TaaI ACNGT 1 cut(s) 930
TaqI TCGA 2 cut(s) 591, 892
TasI AATT 2 cut(s) 291, 946
TfiI GAWTC 3 cut(s) 223, 662, 767
Tru1I TTAA 3 cut(s) 270, 552, 705
Tru9I TTAA 3 cut(s) 270, 552, 705
TscAI CASTG 1 cut(s) 935
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 2 cut(s) 169, 545
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 3 cut(s) 368, 645, 876
TspGWI ACGGA 3 cut(s) 78, 738, 872
TspRI CASTG 1 cut(s) 935
Tth111I GACNNNGTC 1 cut(s) 699
VpaK11BI GGWCC 2 cut(s) 409, 905
XapI RAATTY 1 cut(s) 946
XceI RCATGY 3 cut(s) 13, 47, 373
XspI CTAG 3 cut(s) 227, 443, 961
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.