FvH4_3g28850

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012256
Physical Location & Seq
Forward (+)
21823465 .. 21827020
3556 bp
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UTR
Exon/CDS
Intron
FvH4_3g28850.t1

Sequence Viewer

Length: 666 bp
ATGGTGGGGGCTTTGTCTGTGATGGGTTCATCTGTGGTGGACTCACACACATGTCCATGCTTGTGTTTGGATGCACTGCCTACAACTGCCATGAATCTCAAGAGTGGGGGAGAAGTGGGTTTGCAAAGGAACTCAATGGCCAAGAAGCAGTTAGTGAAGCCAGGAGGGTCTTTGGAGTTGAGCAGTTCTTTCATTGATTCTGGGCATGACAGGTGGCTCTCCATGAAAGCTCTTCCCGGTGTGAGGAGGAAACGAAAGAATCGAAATTTCGTGATTGTTAGTGAGCTTGCTGGCCAATATGAGGACAGCTTTGAGGATGTTAAGACGCAATTACTCAACTATTTTACATACAAAGCAGTGAGGACTGTTATGAACCAGCTATATGAGATGAACCCAACACAGTATAGGTGGTTTTATGACTTTGTTGTATCAAACAAGCCTGGAGATGGCAAGCGGTTCCTCCGCTCCCTTGGGAAGGAGAGGCATGAACTTGCTGAGAGAGTAATGGTCACACGGCTCCACCTCTATGGCAAATGGGTCAAGAAATGTAATCATGCTGAAATATATCAAGAAATTTCTAATGAGAACTTGGACTTGATGCGTGAACGGCTTATTGAGACTGTGATATGGCCCTCAGATGACTCAAACACAGAGAAGATTGGCTAA

Protein Analysis

222

Amino Acids

25.26

Weight (kDa)

8.97

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 107 - 206 2.1e-18 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19855 AT5G19855
fragaria_vesca FvH4_3g28850
malus_domestica MD03G1175000.v1.1 MD11G1193100.v1.1
prunus_persica Prupe.4G249500_v2.0.a1 Prupe.4G249500_v2.0.a1
pyrus_communis pycom03g13250 pycom11g16860
rosa_chinensis RchiOBHm_Chr5g0052051
rosa_laevigata RLG00000034846
rosa_multiflora Rmu_ssc0000098.1_g000001
rosa_roxburghii Rroxscaffold_1G00027850
rosa_rugosa Rorug05G0275200
rosa_samantha Rh5AG347200 Rh5BG358100 Rh5CG383600 Rh5DG373600
rosa_wichuraiana Rw5G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 465
AciI CCGC 2 cut(s) 454, 463
AcoI YGGCCR 2 cut(s) 138, 292
AcsI RAATTY 2 cut(s) 265, 573
AfiI CCNNNNNNNGG 4 cut(s) 243, 301, 446, 475
AflIII ACRYGT 1 cut(s) 50
AjnI CCWGG 2 cut(s) 160, 439
AluBI AGCT 4 cut(s) 230, 286, 309, 379
AluI AGCT 4 cut(s) 230, 286, 309, 379
Alw26I GTCTC 1 cut(s) 611
AoxI GGCC 3 cut(s) 138, 292, 629
ApoI RAATTY 2 cut(s) 265, 573
AspS9I GGNCC 1 cut(s) 630
AsuC2I CCSGG 1 cut(s) 237
BalI TGGCCA 2 cut(s) 140, 294
BccI CCATC 2 cut(s) 16, 440
BceAI ACGGC 2 cut(s) 530, 623
BciT130I CCWGG 2 cut(s) 162, 441
BcnI CCSGG 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 611
Bme1390I CCNGG 3 cut(s) 162, 237, 441
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 2 cut(s) 458, 518
BmrFI CCNGG 3 cut(s) 162, 237, 441
BmsI GCATC 2 cut(s) 61, 588
BpmI CTGGAG 1 cut(s) 462
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 237
BsaJI CCNNGG 1 cut(s) 469
Bsc4I CCNNNNNNNGG 4 cut(s) 243, 301, 446, 475
BseBI CCWGG 2 cut(s) 162, 441
BseDI CCNNGG 1 cut(s) 469
BseGI GGATG 2 cut(s) 76, 322
BseLI CCNNNNNNNGG 4 cut(s) 243, 301, 446, 475
BseMII CTCAG 2 cut(s) 486, 648
BseRI GAGGAG 1 cut(s) 259
BshFI GGCC 3 cut(s) 140, 294, 631
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 4 cut(s) 243, 301, 446, 475
BsmAI GTCTC 1 cut(s) 611
BsnI GGCC 3 cut(s) 140, 294, 631
BspACI CCGC 2 cut(s) 454, 463
BspANI GGCC 3 cut(s) 140, 294, 631
BspCNI CTCAG 2 cut(s) 487, 647
BspLI GGNNCC 2 cut(s) 458, 518
BspQI GCTCTTC 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 465
BssECI CCNNGG 1 cut(s) 469
BssT1I CCWWGG 1 cut(s) 469
Bst2UI CCWGG 2 cut(s) 162, 441
Bst4CI ACNGT 3 cut(s) 367, 402, 622
Bst6I CTCTTC 1 cut(s) 237
BstC8I GCNNGC 3 cut(s) 288, 292, 452
BstDEI CTNAG 2 cut(s) 495, 634
BstENI CCTNNNNNAGG 1 cut(s) 473
BstF5I GGATG 2 cut(s) 76, 322
BstMAI GTCTC 1 cut(s) 611
BstMWI GCNNNNNNNGC 1 cut(s) 607
BstNI CCWGG 2 cut(s) 162, 441
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 3 cut(s) 160, 235, 439
BstXI CCANNNNNNTGG 1 cut(s) 527
BsuRI GGCC 3 cut(s) 140, 294, 631
BtsCI GGATG 2 cut(s) 76, 322
BtsI GCAGTG 2 cut(s) 74, 363
BtsIMutI CAGTG 2 cut(s) 74, 363
Cac8I GCNNGC 3 cut(s) 288, 292, 452
Cfr13I GGNCC 1 cut(s) 630
CseI GACGC 1 cut(s) 334
CviAII CATG 7 cut(s) 51, 57, 91, 206, 223, 485, 554
DdeI CTNAG 2 cut(s) 495, 634
EaeI YGGCCR 2 cut(s) 138, 292
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 469
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 160, 439
EcoT14I CCWWGG 1 cut(s) 469
ErhI CCWWGG 1 cut(s) 469
FaeI CATG 7 cut(s) 54, 60, 94, 209, 226, 488, 557
FatI CATG 7 cut(s) 50, 56, 90, 205, 222, 484, 553
FokI GGATG 2 cut(s) 83, 329
GsuI CTGGAG 1 cut(s) 462
HaeIII GGCC 3 cut(s) 140, 294, 631
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 334
Hin1II CATG 7 cut(s) 54, 60, 94, 209, 226, 488, 557
HinfI GANTC 5 cut(s) 41, 94, 197, 259, 641
HpaII CCGG 1 cut(s) 237
Hpy166II GTNNAC 2 cut(s) 40, 605
Hpy188I TCNGA 1 cut(s) 637
Hpy188III TCNNGA 4 cut(s) 100, 271, 541, 569
Hpy8I GTNNAC 2 cut(s) 40, 605
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 3 cut(s) 367, 402, 622
HpyCH4V TGCA 2 cut(s) 74, 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 607
HpyF3I CTNAG 2 cut(s) 495, 634
Hsp92II CATG 7 cut(s) 54, 60, 94, 209, 226, 488, 557
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 470, 522
LpnPI CCDG 9 cut(s) 147, 174, 186, 196, 250, 276, 389, 426, 453
LweI GCATC 2 cut(s) 61, 588
MaeIII GTNAC 1 cut(s) 508
MbiI CCGCTC 1 cut(s) 465
MboII GAAGA 1 cut(s) 224
MlsI TGGCCA 2 cut(s) 140, 294
MluCI AATT 3 cut(s) 265, 329, 573
MluNI TGGCCA 2 cut(s) 140, 294
MlyI GAGTC 2 cut(s) 35, 635
Mox20I TGGCCA 2 cut(s) 140, 294
MscI TGGCCA 2 cut(s) 140, 294
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 4 cut(s) 49, 55, 61, 525
Msp20I TGGCCA 2 cut(s) 140, 294
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 3 cut(s) 162, 237, 441
MvaI CCWGG 2 cut(s) 162, 441
MwoI GCNNNNNNNGC 1 cut(s) 607
NciI CCSGG 1 cut(s) 237
NlaIII CATG 7 cut(s) 54, 60, 94, 209, 226, 488, 557
NlaIV GGNNCC 2 cut(s) 458, 518
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 1 cut(s) 54
PciI ACATGT 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 3 cut(s) 94, 197, 259
PleI GAGTC 2 cut(s) 35, 635
PpsI GAGTC 2 cut(s) 35, 635
PscI ACATGT 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 160, 439
PspGI CCWGG 2 cut(s) 160, 439
PspN4I GGNNCC 2 cut(s) 458, 518
PspPI GGNCC 1 cut(s) 630
RseI CAYNNNNRTG 4 cut(s) 49, 55, 61, 525
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 1 cut(s) 321
Sau96I GGNCC 1 cut(s) 630
SchI GAGTC 2 cut(s) 35, 635
ScrFI CCNGG 3 cut(s) 162, 237, 441
SetI ASST 7 cut(s) 215, 232, 288, 311, 381, 410, 525
SfaNI GCATC 2 cut(s) 61, 588
SmiMI CAYNNNNRTG 4 cut(s) 49, 55, 61, 525
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 3 cut(s) 265, 329, 573
SsiI CCGC 2 cut(s) 454, 463
StyD4I CCNGG 3 cut(s) 160, 235, 439
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 3 cut(s) 367, 402, 622
TaqI TCGA 1 cut(s) 262
TasI AATT 3 cut(s) 265, 329, 573
TfiI GAWTC 3 cut(s) 94, 197, 259
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 81, 363
TseFI GTSAC 1 cut(s) 508
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 7 cut(s) 18, 107, 181, 239, 386, 404, 501
TspRI CASTG 2 cut(s) 81, 363
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 2 cut(s) 265, 573
XceI RCATGY 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.