pycom03g13250

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012256
Physical Location & Seq
Forward (+)
14186923 .. 14188959
2037 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g13250.1

Sequence Viewer

Length: 678 bp
ATGGTGGGAGCTGCTCTGTCTGTGGTAGGTTCGTCGGTGGTGGACTCGCACACTAGTCCTTGCCTGTGTTTGGATGCGCTGCCGACGACGGCGATGAATCTCAAGAGCGGCGGAGAAATGGTTTTGCAGAGGAATTCAATGGCCAGGAAGCATGTGGTGAAACCAGGCTCCTTGGAGTTGGGCAGTTCGTTCATTGATTTTGGCCACGACAGGCGATTTTCGATGAAAGCTCTTCCTGTTACAGGGAATAGGAGTACGAGGAAGCGAAAGAACCGGGGTTTTGTAATTGTTAATGAACTTGCTGGGCAGTATGAAGACAGTTTTGAGGATGTTAAGACGCAATTACTCAACTATTTTACGTACAAGGCTGTGAGGACTGTTATGAACCAGCTGTACGAGATGAACCCTACGCAATATAGGTGGTTGTACGATTTTGTTGCAACACACAAGCCTGGAGACGGGAAGCGTTTCCTCCGCACCCTTGGGAAGGAGAGGCATGAACTTGCCGAGAGAGTAATGATCACACGGCTTCACCTCTACGGTAAATGGGTCAAGAAATGTATCGATCATGCCGAAATATATCAAGGAATATCAGATGAGAACCTGGAGTTGATGCGTGAACGGCTTTTTGAGACCGTGATATGGCCCTCCGATGACAACACGGAGAAGATTGGCTGA

Protein Analysis

226

Amino Acids

25.74

Weight (kDa)

9.06

Isoelectric Point (pI)

27.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 111 - 210 1.4e-19 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19855 AT5G19855
fragaria_vesca FvH4_3g28850
malus_domestica MD03G1175000.v1.1 MD11G1193100.v1.1
prunus_persica Prupe.4G249500_v2.0.a1 Prupe.4G249500_v2.0.a1
pyrus_communis pycom03g13250 pycom11g16860
rosa_chinensis RchiOBHm_Chr5g0052051
rosa_laevigata RLG00000034846
rosa_multiflora Rmu_ssc0000098.1_g000001
rosa_roxburghii Rroxscaffold_1G00027850
rosa_rugosa Rorug05G0275200
rosa_samantha Rh5AG347200 Rh5BG358100 Rh5CG383600 Rh5DG373600
rosa_wichuraiana Rw5G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 108
AciI CCGC 3 cut(s) 108, 111, 475
AcoI YGGCCR 2 cut(s) 141, 202
AcsI RAATTY 1 cut(s) 133
AfaI GTAC 4 cut(s) 256, 362, 395, 428
AfiI CCNNNNNNNGG 5 cut(s) 70, 242, 458, 487, 642
AgsI TTSAA 1 cut(s) 138
AhlI ACTAGT 1 cut(s) 53
AjnI CCWGG 4 cut(s) 143, 163, 451, 603
AluBI AGCT 3 cut(s) 11, 230, 391
AluI AGCT 3 cut(s) 11, 230, 391
Alw26I GTCTC 2 cut(s) 450, 626
AoxI GGCC 3 cut(s) 141, 202, 644
ApeKI GCWGC 2 cut(s) 11, 79
ApoI RAATTY 1 cut(s) 133
Asp700I GAANNNNTTC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 645
AsuC2I CCSGG 1 cut(s) 275
AsuHPI GGTGA 2 cut(s) 169, 524
BalI TGGCCA 2 cut(s) 143, 204
BbsI GAAGAC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 66
BceAI ACGGC 3 cut(s) 105, 542, 638
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 4 cut(s) 145, 165, 453, 605
BclI TGATCA 1 cut(s) 519
BcnI CCSGG 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 450, 626
BcuI ACTAGT 1 cut(s) 53
BfaI CTAG 1 cut(s) 54
BisI GCNGC 3 cut(s) 12, 80, 109
BlsI GCNGC 3 cut(s) 13, 81, 110
Bme1390I CCNGG 5 cut(s) 145, 165, 275, 453, 605
BmgT120I GGNCC 1 cut(s) 645
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 5 cut(s) 145, 165, 275, 453, 605
BmsI GCATC 2 cut(s) 64, 603
BpiI GAAGAC 1 cut(s) 321
BpmI CTGGAG 2 cut(s) 474, 626
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 3 cut(s) 171, 274, 481
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 242, 458, 487, 642
BseBI CCWGG 4 cut(s) 145, 165, 453, 605
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 171, 274, 481
BseGI GGATG 2 cut(s) 79, 334
BseLI CCNNNNNNNGG 5 cut(s) 70, 242, 458, 487, 642
BseXI GCAGC 1 cut(s) 66
BseYI CCCAGC 1 cut(s) 302
BshFI GGCC 3 cut(s) 143, 204, 646
BshVI ATCGAT 1 cut(s) 564
BsiSI CCGG 1 cut(s) 274
BslI CCNNNNNNNGG 5 cut(s) 70, 242, 458, 487, 642
BsmAI GTCTC 2 cut(s) 450, 626
BsmBI CGTCTC 1 cut(s) 450
BsnI GGCC 3 cut(s) 143, 204, 646
Bso31I GGTCTC 1 cut(s) 626
Bsp143I GATC 2 cut(s) 519, 565
BspACI CCGC 3 cut(s) 108, 111, 475
BspANI GGCC 3 cut(s) 143, 204, 646
BspDI ATCGAT 1 cut(s) 564
BspLI GGNNCC 1 cut(s) 169
BspQI GCTCTTC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 626
BsrBI CCGCTC 1 cut(s) 108
BssECI CCNNGG 3 cut(s) 171, 274, 481
BssMI GATC 2 cut(s) 519, 565
BssT1I CCWWGG 2 cut(s) 171, 481
Bst2UI CCWGG 4 cut(s) 145, 165, 453, 605
Bst4CI ACNGT 4 cut(s) 320, 379, 542, 637
Bst6I CTCTTC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 360
BstENI CCTNNNNNAGG 2 cut(s) 240, 485
BstF5I GGATG 2 cut(s) 79, 334
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 522, 568
BstMAI GTCTC 2 cut(s) 450, 626
BstMBI GATC 2 cut(s) 519, 565
BstMWI GCNNNNNNNGC 1 cut(s) 622
BstNI CCWGG 4 cut(s) 145, 165, 453, 605
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 5 cut(s) 143, 163, 273, 451, 603
BstSNI TACGTA 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 66
BstV2I GAAGAC 1 cut(s) 321
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 3 cut(s) 143, 204, 646
BsuTUI ATCGAT 1 cut(s) 564
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 2 cut(s) 79, 334
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 645
ClaI ATCGAT 1 cut(s) 564
CseI GACGC 1 cut(s) 346
Csp6I GTAC 4 cut(s) 255, 361, 394, 427
CspCI CAANNNNNGTGG 2 cut(s) 401, 436
CviAII CATG 3 cut(s) 152, 497, 569
CviQI GTAC 4 cut(s) 255, 361, 394, 427
DpnI GATC 2 cut(s) 521, 567
DpnII GATC 2 cut(s) 519, 565
EaeI YGGCCR 2 cut(s) 141, 202
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
EciI GGCGGA 1 cut(s) 126
Eco105I TACGTA 1 cut(s) 360
Eco130I CCWWGG 2 cut(s) 171, 481
Eco31I GGTCTC 1 cut(s) 626
EcoNI CCTNNNNNAGG 2 cut(s) 240, 485
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 4 cut(s) 143, 163, 451, 603
EcoT14I CCWWGG 2 cut(s) 171, 481
ErhI CCWWGG 2 cut(s) 171, 481
Esp3I CGTCTC 1 cut(s) 450
FaeI CATG 3 cut(s) 155, 500, 572
FaiI YATR 8 cut(s) 153, 312, 383, 417, 498, 570, 580, 643
FatI CATG 3 cut(s) 151, 496, 568
FbaI TGATCA 1 cut(s) 519
Fnu4HI GCNGC 3 cut(s) 12, 80, 109
FokI GGATG 2 cut(s) 86, 341
Fsp4HI GCNGC 3 cut(s) 12, 80, 109
FspBI CTAG 1 cut(s) 54
GlaI GCGC 1 cut(s) 78
GluI GCNGC 3 cut(s) 12, 80, 109
GsaI CCCAGC 1 cut(s) 306
GsuI CTGGAG 2 cut(s) 474, 626
HaeIII GGCC 3 cut(s) 143, 204, 646
HapII CCGG 1 cut(s) 274
HgaI GACGC 1 cut(s) 346
HhaI GCGC 1 cut(s) 79
Hin1II CATG 3 cut(s) 155, 500, 572
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 2 cut(s) 44, 97
HpaII CCGG 1 cut(s) 274
HphI GGTGA 2 cut(s) 169, 524
Hpy166II GTNNAC 2 cut(s) 43, 620
Hpy188I TCNGA 2 cut(s) 595, 652
Hpy188III TCNNGA 2 cut(s) 103, 553
Hpy8I GTNNAC 2 cut(s) 43, 620
Hpy99I CGWCG 3 cut(s) 37, 88, 91
HpyAV CCTTC 1 cut(s) 481
HpyCH4III ACNGT 4 cut(s) 320, 379, 542, 637
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 2 cut(s) 127, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 622
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 3 cut(s) 155, 500, 572
HspAI GCGC 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 519
Kzo9I GATC 2 cut(s) 519, 565
LguI GCTCTTC 1 cut(s) 237
LmnI GCTCC 2 cut(s) 8, 173
Lsp1109I GCAGC 1 cut(s) 66
LweI GCATC 2 cut(s) 64, 603
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 1 cut(s) 238
MalI GATC 2 cut(s) 521, 567
MbiI CCGCTC 1 cut(s) 108
MboI GATC 2 cut(s) 519, 565
MboII GAAGA 2 cut(s) 224, 326
MlsI TGGCCA 2 cut(s) 143, 204
MluCI AATT 3 cut(s) 133, 285, 341
MluNI TGGCCA 2 cut(s) 143, 204
MlyI GAGTC 1 cut(s) 38
MnlI CCTC 8 cut(s) 123, 252, 319, 366, 482, 486, 545, 658
Mox20I TGGCCA 2 cut(s) 143, 204
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 2 cut(s) 143, 204
MseI TTAA 2 cut(s) 291, 333
Msp20I TGGCCA 2 cut(s) 143, 204
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 1 cut(s) 274
MspR9I CCNGG 5 cut(s) 145, 165, 275, 453, 605
MvaI CCWGG 4 cut(s) 145, 165, 453, 605
MwoI GCNNNNNNNGC 1 cut(s) 622
NciI CCSGG 1 cut(s) 275
NdeII GATC 2 cut(s) 519, 565
NlaIII CATG 3 cut(s) 155, 500, 572
NlaIV GGNNCC 1 cut(s) 169
NmeAIII GCCGAG 1 cut(s) 532
NspI RCATGY 1 cut(s) 155
PciSI GCTCTTC 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 1 cut(s) 97
PkrI GCNGC 3 cut(s) 13, 81, 110
PleI GAGTC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 38
Ppu21I YACGTR 1 cut(s) 360
Psp6I CCWGG 4 cut(s) 143, 163, 451, 603
PspFI CCCAGC 1 cut(s) 302
PspGI CCWGG 4 cut(s) 143, 163, 451, 603
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 1 cut(s) 645
PsrI GAACNNNNNNTAC 2 cut(s) 377, 409
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 4 cut(s) 256, 362, 395, 428
RsaNI GTAC 4 cut(s) 255, 361, 394, 427
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 2 cut(s) 291, 333
SatI GCNGC 3 cut(s) 12, 80, 109
Sau3AI GATC 2 cut(s) 519, 565
Sau96I GGNCC 1 cut(s) 645
SchI GAGTC 1 cut(s) 38
ScrFI CCNGG 5 cut(s) 145, 165, 275, 453, 605
SetI ASST 8 cut(s) 13, 31, 232, 362, 393, 422, 537, 606
SfaNI GCATC 2 cut(s) 64, 603
SmlI CTYRAG 1 cut(s) 101
SmoI CTYRAG 1 cut(s) 101
SnaBI TACGTA 1 cut(s) 360
SpeI ACTAGT 1 cut(s) 53
Sse9I AATT 3 cut(s) 133, 285, 341
SsiI CCGC 3 cut(s) 108, 111, 475
SspMI CTAG 1 cut(s) 54
StyD4I CCNGG 5 cut(s) 143, 163, 273, 451, 603
StyI CCWWGG 2 cut(s) 171, 481
TaaI ACNGT 4 cut(s) 320, 379, 542, 637
TaiI ACGT 1 cut(s) 362
TaqI TCGA 2 cut(s) 221, 564
TasI AATT 3 cut(s) 133, 285, 341
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 97
Tru1I TTAA 2 cut(s) 291, 333
Tru9I TTAA 2 cut(s) 291, 333
TseI GCWGC 2 cut(s) 11, 79
TspDTI ATGAA 8 cut(s) 110, 181, 239, 309, 327, 398, 416, 513
TspGWI ACGGA 1 cut(s) 677
XagI CCTNNNNNAGG 2 cut(s) 240, 485
XapI RAATTY 1 cut(s) 133
XceI RCATGY 1 cut(s) 155
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.