Rh5BG358100

RbcX protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0012256
Physical Location & Seq
Forward (+)
55828465 .. 55832087
3623 bp
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UTR
Exon/CDS
Intron
Rh5BG358100.1

Sequence Viewer

Length: 480 bp
ATGGTGGGGGCTTTATCTGTGATGGGTTCATCCGTGGTGGACTCACACACATGTCCGTGCTTGTGCTTGGATGCTCTGCCTACAACTGCCATGAATCTCAAGAGTGGAGGAGAACAGAGGAACTCAATGGCCAAGAAGCATTTAGTGAAACCAGGAGGGTCTTTGGAGTTGAGCAGTTCATTCATTGATTCAGGCCATGACAGGTGGCTCTCCATGAAAGCTCTTCCGGGTATGAGGAGGAAACGAAAGAATCGAAACTTCGTGATTGTTAATGAGCTTGCAGGCCAATATGAGGACAGCTTTGAGGATGTTAAGACGCAATTACTCAACTATTTTACATACAAGGCTGTGAGGACTGTCATGAACCAGCTTTATGAGATGAACCCAACACAATATAGGTGGTTGTATGACTTTGTTGTATCAAACAAGCCTGGAGATGGTAAGCGGTTCCTCCGCTCACTTGGGAAGGTAGTTTTTTAA

Protein Analysis

159

Amino Acids

17.95

Weight (kDa)

9.54

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RbcX PF02341 104 - 154 9.2e-12 RbcX protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19855 AT5G19855
fragaria_vesca FvH4_3g28850
malus_domestica MD03G1175000.v1.1 MD11G1193100.v1.1
prunus_persica Prupe.4G249500_v2.0.a1 Prupe.4G249500_v2.0.a1
pyrus_communis pycom03g13250 pycom11g16860
rosa_chinensis RchiOBHm_Chr5g0052051
rosa_laevigata RLG00000034846
rosa_multiflora Rmu_ssc0000098.1_g000001
rosa_roxburghii Rroxscaffold_1G00027850
rosa_rugosa Rorug05G0275200
rosa_samantha Rh5AG347200 Rh5BG358100 Rh5CG383600 Rh5DG373600
rosa_wichuraiana Rw5G032810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 456
AciI CCGC 2 cut(s) 445, 454
AcoI YGGCCR 1 cut(s) 129
AfiI CCNNNNNNNGG 2 cut(s) 292, 437
AflIII ACRYGT 1 cut(s) 50
AjnI CCWGG 2 cut(s) 151, 430
AluBI AGCT 4 cut(s) 221, 277, 300, 370
AluI AGCT 4 cut(s) 221, 277, 300, 370
AoxI GGCC 3 cut(s) 129, 193, 283
AsuC2I CCSGG 1 cut(s) 228
BalI TGGCCA 1 cut(s) 131
BccI CCATC 2 cut(s) 16, 431
BciT130I CCWGG 2 cut(s) 153, 432
BcnI CCSGG 1 cut(s) 228
Bme1390I CCNGG 3 cut(s) 153, 228, 432
BmiI GGNNCC 1 cut(s) 449
BmrFI CCNGG 3 cut(s) 153, 228, 432
BmsI GCATC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 292, 437
BseBI CCWGG 2 cut(s) 153, 432
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 29, 76, 313
BseLI CCNNNNNNNGG 2 cut(s) 292, 437
BseRI GAGGAG 2 cut(s) 123, 250
BshFI GGCC 3 cut(s) 131, 195, 285
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 2 cut(s) 292, 437
BsnI GGCC 3 cut(s) 131, 195, 285
BspACI CCGC 2 cut(s) 445, 454
BspANI GGCC 3 cut(s) 131, 195, 285
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 1 cut(s) 449
BspQI GCTCTTC 1 cut(s) 228
BsrBI CCGCTC 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 33
Bst2UI CCWGG 2 cut(s) 153, 432
Bst4CI ACNGT 1 cut(s) 358
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 279, 283
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 29, 76, 313
BstNI CCWGG 2 cut(s) 153, 432
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 3 cut(s) 151, 226, 430
BsuRI GGCC 3 cut(s) 131, 195, 285
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 29, 76, 313
Cac8I GCNNGC 2 cut(s) 279, 283
CciI TCATGA 1 cut(s) 360
CseI GACGC 1 cut(s) 325
CspCI CAANNNNNGTGG 2 cut(s) 380, 415
CviAII CATG 5 cut(s) 51, 91, 197, 214, 361
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 151, 430
FaeI CATG 5 cut(s) 54, 94, 200, 217, 364
FatI CATG 5 cut(s) 50, 90, 196, 213, 360
FokI GGATG 3 cut(s) 16, 83, 320
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 3 cut(s) 131, 195, 285
HapII CCGG 1 cut(s) 227
HgaI GACGC 1 cut(s) 325
Hin1II CATG 5 cut(s) 54, 94, 200, 217, 364
HinfI GANTC 4 cut(s) 41, 94, 188, 250
HpaII CCGG 1 cut(s) 227
Hpy166II GTNNAC 1 cut(s) 40
Hpy188III TCNNGA 3 cut(s) 100, 262, 361
Hpy8I GTNNAC 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 1 cut(s) 358
HpyCH4V TGCA 1 cut(s) 281
Hsp92II CATG 5 cut(s) 54, 94, 200, 217, 364
LguI GCTCTTC 1 cut(s) 228
LpnPI CCDG 9 cut(s) 138, 165, 177, 187, 240, 267, 380, 417, 444
LweI GCATC 1 cut(s) 61
MbiI CCGCTC 1 cut(s) 456
MboII GAAGA 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 1 cut(s) 320
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 9 cut(s) 101, 111, 149, 228, 231, 286, 298, 345, 461
Mox20I TGGCCA 1 cut(s) 131
MscI TGGCCA 1 cut(s) 131
MseI TTAA 3 cut(s) 270, 312, 478
MslI CAYNNNNRTG 2 cut(s) 49, 55
Msp20I TGGCCA 1 cut(s) 131
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 3 cut(s) 153, 228, 432
MvaI CCWGG 2 cut(s) 153, 432
NciI CCSGG 1 cut(s) 228
NlaIII CATG 5 cut(s) 54, 94, 200, 217, 364
NlaIV GGNNCC 1 cut(s) 449
NspI RCATGY 1 cut(s) 54
PagI TCATGA 1 cut(s) 360
PciI ACATGT 1 cut(s) 50
PciSI GCTCTTC 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PfeI GAWTC 3 cut(s) 94, 188, 250
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PscI ACATGT 1 cut(s) 50
Psp6I CCWGG 2 cut(s) 151, 430
PspGI CCWGG 2 cut(s) 151, 430
PspN4I GGNNCC 1 cut(s) 449
RseI CAYNNNNRTG 2 cut(s) 49, 55
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 3 cut(s) 270, 312, 478
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 3 cut(s) 153, 228, 432
SetI ASST 7 cut(s) 206, 223, 279, 302, 372, 401, 471
SfaNI GCATC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 49, 55
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 1 cut(s) 320
SsiI CCGC 2 cut(s) 445, 454
StyD4I CCNGG 3 cut(s) 151, 226, 430
TaaI ACNGT 1 cut(s) 358
TaqI TCGA 1 cut(s) 253
TasI AATT 1 cut(s) 320
TfiI GAWTC 3 cut(s) 94, 188, 250
Tru1I TTAA 3 cut(s) 270, 312, 478
Tru9I TTAA 3 cut(s) 270, 312, 478
TspDTI ATGAA 7 cut(s) 18, 107, 168, 172, 230, 377, 395
TspGWI ACGGA 2 cut(s) 22, 45
XceI RCATGY 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.