FvH4_3g29360

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014888
Physical Location & Seq
Reverse (-)
22455245 .. 22455805
561 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29360.t1

Sequence Viewer

Length: 561 bp
ATGGCGGACTCCGACAACGACTCCGGAGGCGGGCACAACAGCCCCAAGAGCGAGCTCTCGCCGCGGGAGCAGGACCGGCTGCTGCCCATTGCCAACGTCAGCAGGATCATGAAGAAGGCTCTGCCGGCCAACGCCAAGATCTCCAAGGACGCCAAGGAGACTGTGCAGGAGTGTGTCTCCGAGTTCATCAGCTTCATCACCGGAGAGGCCTCCGACAAGTGCCAGCGCGAGAAGCGCAAGACCATCAACGGCGACGATCTCCTCTGGGCCATGACGACGTTAGGGTTCGAGGAGTATGTGGAGCCGCTGAAAGTGTACTTGCAGCGGTTTAGGGAGATGGAGGGGGAGAAGAGCAGCATGGCGGCGCGTGATAAGGACTCGAGCGGCGGGGTTGTGGGGTTTGAGGGAGGAGGAGCAGGAGCAGGAGGAGGGGGAGTCTATTGGCAGCAGCAGCCGCAGCCGGGACACGTGTACGGCGGCGGGGGGTTTCATCAGATGGGTGTTGGTGGGGTTGGGATTGGAAAGGGGGGGTCTGGGACCAACATGGGTAGACCCAGATGA

Protein Analysis

187

Amino Acids

19.76

Weight (kDa)

6.32

Isoelectric Point (pI)

41.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 22 - 91 4.9e-06 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 27 - 91 1.9e-27 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014888)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47640
fragaria_vesca FvH4_3g29360
malus_domestica MD03G1183400.v1.1 MD11G1200400.v1.1
prunus_persica Prupe.4G242700_v2.0.a1
pyrus_communis pycom03g13700 pycom11g17280
rosa_chinensis RchiOBHm_Chr5g0053731
rosa_laevigata RLG00000034898
rosa_multiflora Rmu_sc0001759.1_g000047
rosa_roxburghii Rroxscaffold_1G00026870
rosa_rugosa Rorug05G0281800
rosa_samantha Rh5AG352700 Rh5BG364400 Rh5DG379200
rosa_wichuraiana Rw5G033320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 384
AccI GTMKAC 1 cut(s) 550
AccII CGCG 3 cut(s) 64, 228, 367
AccIII TCCGGA 1 cut(s) 23
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 126
AcvI CACGTG 1 cut(s) 469
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 2 cut(s) 317, 473
AfiI CCNNNNNNNGG 2 cut(s) 30, 461
AflIII ACRYGT 2 cut(s) 466, 468
AluBI AGCT 2 cut(s) 55, 192
AluI AGCT 2 cut(s) 55, 192
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 2 cut(s) 152, 181
AlwI GGATC 1 cut(s) 113
Ama87I CYCGRG 1 cut(s) 379
Aor13HI TCCGGA 1 cut(s) 23
AoxI GGCC 3 cut(s) 126, 207, 267
ApeKI GCWGC 8 cut(s) 79, 82, 322, 354, 445, 448, 451, 457
AspLEI GCGC 3 cut(s) 228, 237, 367
AspS9I GGNCC 3 cut(s) 73, 267, 537
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 190
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 2 cut(s) 73, 537
BaeGI GKGCMC 1 cut(s) 36
BanII GRGCYC 1 cut(s) 57
BbrPI CACGTG 1 cut(s) 469
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 8 cut(s) 66, 69, 334, 366, 457, 460, 463, 469
BccI CCATC 3 cut(s) 251, 331, 490
BceAI ACGGC 2 cut(s) 265, 490
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 152, 181
BglII AGATCT 1 cut(s) 138
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 2 cut(s) 73, 537
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 3 cut(s) 73, 267, 537
BmiI GGNNCC 2 cut(s) 303, 538
BmrFI CCNGG 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 161, 193
BpuMI CCSGG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 469
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 62, 144, 153
BsaWI WCCGGW 2 cut(s) 23, 200
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 420, 450
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 461
Bse118I RCCGGY 2 cut(s) 75, 124
Bse3DI GCAATG 1 cut(s) 87
BseAI TCCGGA 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 62, 144, 153
BseLI CCNNNNNNNGG 2 cut(s) 30, 461
BseMI GCAATG 1 cut(s) 87
BseRI GAGGAG 5 cut(s) 251, 305, 423, 426, 441
BseSI GKGCMC 1 cut(s) 36
BseXI GCAGC 8 cut(s) 66, 69, 334, 366, 457, 460, 463, 469
BsgI GTGCAG 1 cut(s) 185
Bsh1236I CGCG 3 cut(s) 64, 228, 367
BshFI GGCC 3 cut(s) 128, 209, 269
BsiHKAI GWGCWC 1 cut(s) 57
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 5 cut(s) 24, 76, 125, 201, 461
BslFI GGGAC 2 cut(s) 477, 550
BslI CCNNNNNNNGG 2 cut(s) 30, 461
BsmAI GTCTC 2 cut(s) 152, 181
BsmFI GGGAC 2 cut(s) 477, 550
BsnI GGCC 3 cut(s) 128, 209, 269
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 36, 57
Bsp13I TCCGGA 1 cut(s) 23
Bsp143I GATC 3 cut(s) 105, 138, 256
BspANI GGCC 3 cut(s) 128, 209, 269
BspEI TCCGGA 1 cut(s) 23
BspFNI CGCG 3 cut(s) 64, 228, 367
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 303, 538
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 344
BsrBI CCGCTC 1 cut(s) 384
BsrDI GCAATG 1 cut(s) 87
BsrFI RCCGGY 2 cut(s) 75, 124
BssAI RCCGGY 2 cut(s) 75, 124
BssECI CCNNGG 3 cut(s) 62, 144, 153
BssMI GATC 3 cut(s) 105, 138, 256
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 144, 153
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 1 cut(s) 150
BstBAI YACGTR 1 cut(s) 469
BstC8I GCNNGC 4 cut(s) 32, 53, 126, 224
BstDSI CCRYGG 1 cut(s) 62
BstFNI CGCG 3 cut(s) 64, 228, 367
BstHHI GCGC 3 cut(s) 228, 237, 367
BstKTI GATC 3 cut(s) 108, 141, 259
BstMAI GTCTC 2 cut(s) 152, 181
BstMBI GATC 3 cut(s) 105, 138, 256
BstSCI CCNGG 1 cut(s) 460
BstSLI GKGCMC 1 cut(s) 36
BstUI CGCG 3 cut(s) 64, 228, 367
BstV1I GCAGC 8 cut(s) 66, 69, 334, 366, 457, 460, 463, 469
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 3 cut(s) 128, 209, 269
BtgI CCRYGG 1 cut(s) 62
Cac8I GCNNGC 4 cut(s) 32, 53, 126, 224
CciI TCATGA 1 cut(s) 108
CfoI GCGC 3 cut(s) 228, 237, 367
Cfr10I RCCGGY 2 cut(s) 75, 124
Cfr13I GGNCC 3 cut(s) 73, 267, 537
Cfr42I CCGCGG 1 cut(s) 65
CseI GACGC 1 cut(s) 158
Csp6I GTAC 2 cut(s) 316, 472
CviAII CATG 4 cut(s) 109, 271, 358, 544
CviQI GTAC 2 cut(s) 316, 472
DpnI GATC 3 cut(s) 107, 140, 258
DpnII GATC 3 cut(s) 105, 138, 256
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 2 cut(s) 144, 153
Eco147I AGGCCT 1 cut(s) 209
Eco24I GRGCYC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 73, 537
Eco53kI GAGCTC 1 cut(s) 55
Eco72I CACGTG 1 cut(s) 469
Eco88I CYCGRG 1 cut(s) 379
EcoICRI GAGCTC 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 144, 153
EcoT38I GRGCYC 1 cut(s) 57
ErhI CCWWGG 2 cut(s) 144, 153
FaeI CATG 4 cut(s) 112, 274, 361, 547
FaiI YATR 5 cut(s) 110, 272, 297, 359, 545
FaqI GGGAC 2 cut(s) 477, 550
FatI CATG 4 cut(s) 108, 270, 357, 543
FauI CCCGC 4 cut(s) 23, 57, 380, 473
FblI GTMKAC 1 cut(s) 550
FriOI GRGCYC 1 cut(s) 57
GlaI GCGC 3 cut(s) 227, 236, 366
HaeIII GGCC 3 cut(s) 128, 209, 269
HapII CCGG 5 cut(s) 24, 76, 125, 201, 461
HgaI GACGC 1 cut(s) 158
HhaI GCGC 3 cut(s) 228, 237, 367
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 4 cut(s) 112, 274, 361, 547
Hin6I GCGC 3 cut(s) 226, 235, 365
HinP1I GCGC 3 cut(s) 226, 235, 365
HinfI GANTC 4 cut(s) 8, 20, 377, 435
HpaII CCGG 5 cut(s) 24, 76, 125, 201, 461
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 3 cut(s) 316, 472, 551
Hpy188I TCNGA 4 cut(s) 13, 181, 214, 495
Hpy188III TCNNGA 2 cut(s) 24, 109
Hpy8I GTNNAC 3 cut(s) 316, 472, 551
Hpy99I CGWCG 2 cut(s) 257, 280
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 3 cut(s) 96, 278, 468
HpyCH4V TGCA 2 cut(s) 166, 322
HpySE526I ACGT 3 cut(s) 96, 278, 468
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 4 cut(s) 112, 274, 361, 547
HspAI GCGC 3 cut(s) 226, 235, 365
Kpn2I TCCGGA 1 cut(s) 23
KroI GCCGGC 1 cut(s) 124
KroNI GCCGGC 1 cut(s) 126
KspI CCGCGG 1 cut(s) 65
Kzo9I GATC 3 cut(s) 105, 138, 256
LguI GCTCTTC 1 cut(s) 344
LmnI GCTCC 4 cut(s) 67, 301, 413, 419
Lsp1109I GCAGC 8 cut(s) 66, 69, 334, 366, 457, 460, 463, 469
MaeII ACGT 3 cut(s) 96, 278, 468
MalI GATC 3 cut(s) 107, 140, 258
MbiI CCGCTC 1 cut(s) 384
MboI GATC 3 cut(s) 105, 138, 256
MboII GAAGA 2 cut(s) 124, 361
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 2 cut(s) 36, 57
MlyI GAGTC 4 cut(s) 2, 14, 371, 444
MmeI TCCRAC 2 cut(s) 36, 237
MroI TCCGGA 1 cut(s) 23
MroNI GCCGGC 1 cut(s) 124
MspA1I CMGCKG 3 cut(s) 64, 307, 325
MspI CCGG 5 cut(s) 24, 76, 125, 201, 461
MspR9I CCNGG 1 cut(s) 462
MvnI CGCG 3 cut(s) 64, 228, 367
NaeI GCCGGC 1 cut(s) 126
NciI CCSGG 1 cut(s) 462
NdeII GATC 3 cut(s) 105, 138, 256
NgoMIV GCCGGC 1 cut(s) 124
NlaIII CATG 4 cut(s) 112, 274, 361, 547
NlaIV GGNNCC 2 cut(s) 303, 538
PaeR7I CTCGAG 1 cut(s) 379
PagI TCATGA 1 cut(s) 108
PceI AGGCCT 1 cut(s) 209
PciSI GCTCTTC 1 cut(s) 344
PdiI GCCGGC 1 cut(s) 126
PleI GAGTC 4 cut(s) 2, 14, 371, 443
PmaCI CACGTG 1 cut(s) 469
PmlI CACGTG 1 cut(s) 469
PpsI GAGTC 4 cut(s) 2, 14, 371, 443
Ppu21I YACGTR 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 57
PspCI CACGTG 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 303, 538
PspPI GGNCC 3 cut(s) 73, 267, 537
PspXI VCTCGAGB 1 cut(s) 379
PsuI RGATCY 1 cut(s) 138
RsaI GTAC 2 cut(s) 317, 473
RsaNI GTAC 2 cut(s) 316, 472
SacI GAGCTC 1 cut(s) 57
SacII CCGCGG 1 cut(s) 65
SapI GCTCTTC 1 cut(s) 344
Sau3AI GATC 3 cut(s) 105, 138, 256
Sau96I GGNCC 3 cut(s) 73, 267, 537
SchI GAGTC 4 cut(s) 2, 14, 371, 444
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 2 cut(s) 36, 57
SetI ASST 5 cut(s) 57, 99, 194, 281, 471
Sfr274I CTCGAG 1 cut(s) 379
Sfr303I CCGCGG 1 cut(s) 65
SgrBI CCGCGG 1 cut(s) 65
SinI GGWCC 2 cut(s) 73, 537
SlaI CTCGAG 1 cut(s) 379
SmlI CTYRAG 1 cut(s) 379
SmoI CTYRAG 1 cut(s) 379
SseBI AGGCCT 1 cut(s) 209
SstI GAGCTC 1 cut(s) 57
StuI AGGCCT 1 cut(s) 209
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 2 cut(s) 144, 153
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 3 cut(s) 99, 281, 471
TaqI TCGA 2 cut(s) 288, 380
TatI WGTACW 1 cut(s) 315
TauI GCSGC 6 cut(s) 64, 307, 365, 387, 457, 480
TseI GCWGC 8 cut(s) 79, 82, 322, 354, 445, 448, 451, 457
TspDTI ATGAA 4 cut(s) 125, 175, 184, 479
VpaK11BI GGWCC 2 cut(s) 73, 537
XhoI CTCGAG 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.