FvH4_3g30070

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008659
Physical Location & Seq
Reverse (-)
23297306 .. 23301477
4172 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30070.t3

Sequence Viewer

Length: 1635 bp
ATGCGGGTGGAATTGACTTCAAGAAACATAGTAATGGTGTACTGTGCAGCGGACTACCTTGAAATGATAGATGAGTTTGGAGAAGAGAACTTACTCTCAAAGTCGGAGAACTTTTTCTATAAGAATATACTGCACAACTGGAAAGACTGTATCTTGGCTCTTCAAAGTTCTGAGCCCGTTCTTCAAAGGGCAGAAAAACTTCACATAATAAACAAATGTGTAAATGCTTTATCTGTGATGGTTTGCACAGATCCTAGTTTGTTTGGATGGCCCATGATGATGTATGGTAGCTTACAGAGTCCTGGTGGAAGCATCCTGTGGAATGGAATTAATACTGGAGCAAGGATTCGAAGCTCTGAGTCTGATTGGTGGTTTGAAGACATCTCATACCTCAGTGTGAGTTTGTTTGAGAGACTTATCAAGGCAATGGAGATGAAGGGTATAAGACCTGAAAACATCGCAGGTGCTGTAATGCACTATACCAGAAAATACCTACCCGGCCTGGGCAGATGGCAGGGTGGACAACATGGTATACCCAGGACAGTTACAAGTTTTAGTTTTACACCTATTGCCCTAGATCAAAGGGTAGTTTTAGAAAGCATTGAGAAACTTCTTCCCCAAAAGAAGGGTAAATCATTTTGCCGGTTTTTGCTCGGACTTCTTCGTGTTGCATTAATATTGGGAGCCAGTCAAACCTGCAAGGACTCTTTGGAAAGGAGAATAGGAAGGCAACTGGAATTTGCAACTTTAGATGGTCTTCTGATTCCGACATATTCAGATGCTGAGACATTGTATGATACTGACACTGTTCAGCGGATAATCCAACACTTCATGTCATCAGAATCGAAGGTTACACCATTTTCTCCAGCATCAGTTGAAGTGGAAACATCTCCATCGTCAGGTCCGTTAAGGTCAGTTGCAAAGCTGATAGATAGCTACATTGCGGAGGTTGCTTCAGATGTTACTTTAAAGCCTGAAAAGATACGCTCTCTTGCAGTGGCTCTTCCAGAATCGTCAAGAACTTTGCATGATGGGTTATATAGGTCACTTGATATATACTTCAAGGCGCATCCTTGGCTCTCAGATAAGGAGAAGGAAGAACTTTGCAACATCATTGACTTCCAGAAACTTTCTATTGATGCCTGTGCCCATGCATCCCAAAATGAAAGGTTGCCACTCAGAGTTGTTCTTCAAGTCCTGTTCTTTGAGCAGATGCATTTGAGAACTGCTCTAGCTGGTCATCTGAATGTCTTGGAAACTGAAAGTGCTCCTGCAGGTCCTGTTAATCTCCATGCTGATACGGCTGGCCAGATTATACAGAGGGATGGGTGGGTGACTATGGTGCGTGAGAACCGGGTTTTAAAGGTGGATATGGACAAGATGAGATCTAGAGTTGGTGAGCTCGAACAAGAATTTAGTAAAATAAAGCAAGAGATGAAAAAGGTGACCAAATCACATAGTTCCATCAGTTCGCCTCGTGTGGTTGCCAGGAGAATTGGTTGCAATCTTCTTTCACGACCTTCAGATGCTAAACCAGACACTATTGGAAGTACTGGGCACTCCCCAAGAGCATCAGTTGAGAAGGCACGTTTTTCCTGCCATTCTAGACACAAAAAAAGTTTCTCATTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

545

Amino Acids

61.25

Weight (kDa)

8.66

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 133 - 393 6.3e-89 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 452
Acc36I ACCTGC 3 cut(s) 452, 704, 1265
AccI GTMKAC 1 cut(s) 532
AciI CCGC 4 cut(s) 4, 50, 814, 944
AclWI GGATC 1 cut(s) 245
AcoI YGGCCR 1 cut(s) 1306
AcsI RAATTY 2 cut(s) 737, 1412
AcuI CTGAAG 2 cut(s) 939, 1506
AfaI GTAC 2 cut(s) 41, 1552
AfiI CCNNNNNNNGG 3 cut(s) 503, 625, 899
AgsI TTSAA 8 cut(s) 21, 62, 164, 185, 377, 878, 1063, 1193
AjnI CCWGG 4 cut(s) 301, 501, 536, 1487
AluBI AGCT 6 cut(s) 291, 354, 925, 936, 1235, 1402
AluI AGCT 6 cut(s) 291, 354, 925, 936, 1235, 1402
Alw21I GWGCWC 2 cut(s) 1270, 1404
Alw26I GTCTC 2 cut(s) 406, 779
AlwI GGATC 1 cut(s) 245
AlwNI CAGNNNCTG 3 cut(s) 467, 782, 1280
AoxI GGCC 3 cut(s) 269, 499, 1306
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 737, 1412
AseI ATTAAT 2 cut(s) 330, 674
Asp700I GAANNNNTTC 2 cut(s) 113, 198
AspLEI GCGC 1 cut(s) 1069
AspS9I GGNCC 3 cut(s) 270, 902, 1277
AsuC2I CCSGG 2 cut(s) 498, 1355
AsuHPI GGTGA 3 cut(s) 1345, 1409, 1456
AsuII TTCGAA 1 cut(s) 349
AvaII GGWCC 2 cut(s) 902, 1277
BaeGI GKGCMC 2 cut(s) 1150, 1560
BalI TGGCCA 1 cut(s) 1308
BanII GRGCYC 2 cut(s) 177, 1404
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BauI CACGAG 1 cut(s) 1476
BbsI GAAGAC 2 cut(s) 384, 749
Bbv12I GWGCWC 2 cut(s) 1270, 1404
BbvI GCAGC 1 cut(s) 59
BccI CCATC 8 cut(s) 232, 261, 504, 746, 901, 1025, 1319, 1472
BceAI ACGGC 1 cut(s) 1317
BciT130I CCWGG 4 cut(s) 303, 503, 538, 1489
BcnI CCSGG 2 cut(s) 498, 1355
BcoDI GTCTC 2 cut(s) 406, 779
BfaI CTAG 5 cut(s) 255, 575, 1232, 1389, 1605
BfmI CTRYAG 1 cut(s) 1272
BfuAI ACCTGC 3 cut(s) 452, 704, 1265
BglII AGATCT 1 cut(s) 1385
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
BmcAI AGTACT 1 cut(s) 1552
Bme1390I CCNGG 6 cut(s) 303, 498, 503, 538, 1355, 1489
Bme18I GGWCC 2 cut(s) 902, 1277
BmgT120I GGNCC 3 cut(s) 270, 902, 1277
BmiI GGNNCC 1 cut(s) 685
BmrFI CCNGG 6 cut(s) 303, 498, 503, 538, 1355, 1489
BmrI ACTGGG 1 cut(s) 1563
BmsI GCATC 9 cut(s) 321, 769, 878, 1078, 1129, 1163, 1203, 1516, 1580
BmuI ACTGGG 1 cut(s) 1563
BpiI GAAGAC 2 cut(s) 384, 749
BplI GAGNNNNNCTC 2 cut(s) 1213, 1245
BpmI CTGGAG 2 cut(s) 357, 849
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 2 cut(s) 498, 1355
BsaJI CCNNGG 3 cut(s) 502, 536, 1073
BsaXI ACNNNNNCTCC 2 cut(s) 1483, 1513
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 625, 899
Bse118I RCCGGY 1 cut(s) 642
Bse1I ACTGG 5 cut(s) 143, 340, 687, 738, 1558
Bse3DI GCAATG 2 cut(s) 432, 939
BseBI CCWGG 4 cut(s) 303, 503, 538, 1489
BseDI CCNNGG 3 cut(s) 502, 536, 1073
BseGI GGATG 5 cut(s) 272, 312, 1069, 1154, 1330
BseLI CCNNNNNNNGG 3 cut(s) 503, 625, 899
BseMI GCAATG 2 cut(s) 432, 939
BseMII CTCAG 6 cut(s) 162, 348, 406, 774, 1095, 1192
BseNI ACTGG 5 cut(s) 143, 340, 687, 738, 1558
BseSI GKGCMC 2 cut(s) 1150, 1560
BseXI GCAGC 1 cut(s) 59
BsgI GTGCAG 2 cut(s) 66, 116
BshFI GGCC 3 cut(s) 271, 501, 1308
BsiHKAI GWGCWC 2 cut(s) 1270, 1404
BsiSI CCGG 3 cut(s) 498, 643, 1354
BslI CCNNNNNNNGG 3 cut(s) 503, 625, 899
BsmAI GTCTC 2 cut(s) 406, 779
BsnI GGCC 3 cut(s) 271, 501, 1308
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 5 cut(s) 177, 1150, 1270, 1404, 1560
Bsp143I GATC 3 cut(s) 250, 577, 1385
BspACI CCGC 4 cut(s) 4, 50, 814, 944
BspANI GGCC 3 cut(s) 271, 501, 1308
BspCNI CTCAG 6 cut(s) 163, 349, 405, 775, 1094, 1191
BspLI GGNNCC 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 1276
BspMI ACCTGC 3 cut(s) 452, 704, 1265
BspPI GGATC 1 cut(s) 245
BspQI GCTCTTC 2 cut(s) 165, 1008
BspT104I TTCGAA 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 432, 939
BsrFI RCCGGY 1 cut(s) 642
BsrI ACTGG 5 cut(s) 143, 340, 687, 738, 1558
BssAI RCCGGY 1 cut(s) 642
BssECI CCNNGG 3 cut(s) 502, 536, 1073
BssMI GATC 3 cut(s) 250, 577, 1385
BssNAI GTATAC 1 cut(s) 533
BssSI CACGAG 1 cut(s) 1476
BssT1I CCWWGG 1 cut(s) 1073
Bst1107I GTATAC 1 cut(s) 533
Bst2BI CACGAG 1 cut(s) 1476
Bst2UI CCWGG 4 cut(s) 303, 503, 538, 1489
Bst4CI ACNGT 4 cut(s) 44, 149, 544, 808
Bst6I CTCTTC 3 cut(s) 78, 165, 1008
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 1306
BstDEI CTNAG 6 cut(s) 171, 357, 392, 783, 1081, 1178
BstEII GGTNACC 1 cut(s) 1444
BstF5I GGATG 5 cut(s) 272, 312, 1069, 1154, 1330
BstHHI GCGC 1 cut(s) 1069
BstKTI GATC 3 cut(s) 253, 580, 1388
BstMAI GTCTC 2 cut(s) 406, 779
BstMBI GATC 3 cut(s) 250, 577, 1385
BstMWI GCNNNNNNNGC 3 cut(s) 950, 1075, 1301
BstNI CCWGG 4 cut(s) 303, 503, 538, 1489
BstPI GGTNACC 1 cut(s) 1444
BstSCI CCNGG 6 cut(s) 301, 496, 501, 536, 1353, 1487
BstSFI CTRYAG 1 cut(s) 1272
BstSLI GKGCMC 2 cut(s) 1150, 1560
BstV1I GCAGC 1 cut(s) 59
BstV2I GAAGAC 2 cut(s) 384, 749
BstX2I RGATCY 2 cut(s) 250, 1385
BstYI RGATCY 2 cut(s) 250, 1385
BstZ17I GTATAC 1 cut(s) 533
BsuRI GGCC 3 cut(s) 271, 501, 1308
BtgZI GCGATG 1 cut(s) 442
BtsCI GGATG 5 cut(s) 272, 312, 1069, 1154, 1330
BtsI GCAGTG 1 cut(s) 1002
BtsIMutI CAGTG 3 cut(s) 400, 804, 1002
BveI ACCTGC 3 cut(s) 452, 704, 1265
Cac8I GCNNGC 1 cut(s) 1306
CaiI CAGNNNCTG 3 cut(s) 467, 782, 1280
CfoI GCGC 1 cut(s) 1069
Cfr10I RCCGGY 1 cut(s) 642
Cfr13I GGNCC 3 cut(s) 270, 902, 1277
Csp6I GTAC 2 cut(s) 40, 1551
CviAII CATG 6 cut(s) 274, 527, 832, 1028, 1151, 1292
CviQI GTAC 2 cut(s) 40, 1551
DdeI CTNAG 6 cut(s) 171, 357, 392, 783, 1081, 1178
DpnI GATC 3 cut(s) 252, 579, 1387
DpnII GATC 3 cut(s) 250, 577, 1385
DraI TTTAAA 2 cut(s) 969, 1362
EaeI YGGCCR 1 cut(s) 1306
Eam1104I CTCTTC 3 cut(s) 78, 165, 1008
EarI CTCTTC 3 cut(s) 78, 165, 1008
Ecl136II GAGCTC 1 cut(s) 1402
Eco130I CCWWGG 1 cut(s) 1073
Eco24I GRGCYC 2 cut(s) 177, 1404
Eco47I GGWCC 2 cut(s) 902, 1277
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 2 cut(s) 939, 1506
Eco91I GGTNACC 1 cut(s) 1444
EcoICRI GAGCTC 1 cut(s) 1402
EcoO109I RGGNCCY 1 cut(s) 1277
EcoO65I GGTNACC 1 cut(s) 1444
EcoRII CCWGG 4 cut(s) 301, 501, 536, 1487
EcoT14I CCWWGG 1 cut(s) 1073
EcoT22I ATGCAT 2 cut(s) 1156, 1218
EcoT38I GRGCYC 2 cut(s) 177, 1404
ErhI CCWWGG 1 cut(s) 1073
FaeI CATG 6 cut(s) 277, 530, 835, 1031, 1154, 1295
FatI CATG 6 cut(s) 273, 526, 831, 1027, 1150, 1291
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 5 cut(s) 279, 299, 1056, 1141, 1337
FriOI GRGCYC 2 cut(s) 177, 1404
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 5 cut(s) 255, 575, 1232, 1389, 1605
GlaI GCGC 1 cut(s) 1068
GluI GCNGC 1 cut(s) 48
GsuI CTGGAG 2 cut(s) 357, 849
HaeIII GGCC 3 cut(s) 271, 501, 1308
HapII CCGG 3 cut(s) 498, 643, 1354
HhaI GCGC 1 cut(s) 1069
Hin1II CATG 6 cut(s) 277, 530, 835, 1031, 1154, 1295
Hin6I GCGC 1 cut(s) 1067
HinP1I GCGC 1 cut(s) 1067
HinfI GANTC 7 cut(s) 298, 346, 359, 704, 763, 842, 1010
HpaII CCGG 3 cut(s) 498, 643, 1354
HphI GGTGA 3 cut(s) 1345, 1409, 1456
Hpy166II GTNNAC 3 cut(s) 40, 521, 533
Hpy188III TCNNGA 7 cut(s) 21, 1007, 1017, 1123, 1389, 1515, 1605
Hpy8I GTNNAC 3 cut(s) 40, 521, 533
HpyAV CCTTC 7 cut(s) 430, 619, 720, 841, 1087, 1530, 1576
HpyCH4III ACNGT 4 cut(s) 44, 149, 544, 808
HpyCH4IV ACGT 1 cut(s) 1588
HpyF10VI GCNNNNNNNGC 3 cut(s) 950, 1075, 1301
HpyF3I CTNAG 6 cut(s) 171, 357, 392, 783, 1081, 1178
HpySE526I ACGT 1 cut(s) 1588
Hsp92II CATG 6 cut(s) 277, 530, 835, 1031, 1154, 1295
HspAI GCGC 1 cut(s) 1067
Kzo9I GATC 3 cut(s) 250, 577, 1385
LguI GCTCTTC 2 cut(s) 165, 1008
LmnI GCTCC 3 cut(s) 338, 683, 1273
Lsp1109I GCAGC 1 cut(s) 59
LweI GCATC 9 cut(s) 321, 769, 878, 1078, 1129, 1163, 1203, 1516, 1580
MaeI CTAG 5 cut(s) 255, 575, 1232, 1389, 1605
MaeII ACGT 1 cut(s) 1588
MaeIII GTNAC 6 cut(s) 544, 850, 961, 1044, 1333, 1444
MalI GATC 3 cut(s) 252, 579, 1387
MboI GATC 3 cut(s) 250, 577, 1385
MflI RGATCY 2 cut(s) 250, 1385
MhlI GDGCHC 5 cut(s) 177, 1150, 1270, 1404, 1560
MlsI TGGCCA 1 cut(s) 1308
MluCI AATT 5 cut(s) 11, 327, 737, 1412, 1494
MluNI TGGCCA 1 cut(s) 1308
MlyI GAGTC 3 cut(s) 307, 368, 698
MmeI TCCRAC 3 cut(s) 84, 791, 847
MnlI CCTC 4 cut(s) 401, 940, 1314, 1485
Mox20I TGGCCA 1 cut(s) 1308
Mph1103I ATGCAT 2 cut(s) 1156, 1218
MroXI GAANNNNTTC 2 cut(s) 113, 198
MscI TGGCCA 1 cut(s) 1308
MseI TTAA 6 cut(s) 330, 674, 908, 968, 1284, 1361
MslI CAYNNNNRTG 3 cut(s) 32, 278, 1245
Msp20I TGGCCA 1 cut(s) 1308
MspA1I CMGCKG 2 cut(s) 50, 814
MspI CCGG 3 cut(s) 498, 643, 1354
MspR9I CCNGG 6 cut(s) 303, 498, 503, 538, 1355, 1489
MvaI CCWGG 4 cut(s) 303, 503, 538, 1489
MwoI GCNNNNNNNGC 3 cut(s) 950, 1075, 1301
NciI CCSGG 2 cut(s) 498, 1355
NdeII GATC 3 cut(s) 250, 577, 1385
NlaIII CATG 6 cut(s) 277, 530, 835, 1031, 1154, 1295
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 3 cut(s) 1044, 1333, 1444
NsiI ATGCAT 2 cut(s) 1156, 1218
NspV TTCGAA 1 cut(s) 349
PaqCI CACCTGC 1 cut(s) 452
PciSI GCTCTTC 2 cut(s) 165, 1008
PcsI WCGNNNNNNNCGW 1 cut(s) 902
PdmI GAANNNNTTC 2 cut(s) 113, 198
PfeI GAWTC 4 cut(s) 346, 763, 842, 1010
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 3 cut(s) 306, 367, 698
PpsI GAGTC 3 cut(s) 306, 367, 698
PpuMI RGGWCCY 1 cut(s) 1277
PshBI ATTAAT 2 cut(s) 330, 674
Psp124BI GAGCTC 1 cut(s) 1404
Psp5II RGGWCCY 1 cut(s) 1277
Psp6I CCWGG 4 cut(s) 301, 501, 536, 1487
PspEI GGTNACC 1 cut(s) 1444
PspGI CCWGG 4 cut(s) 301, 501, 536, 1487
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 3 cut(s) 270, 902, 1277
PspPPI RGGWCCY 1 cut(s) 1277
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 3 cut(s) 467, 782, 1280
PsuI RGATCY 2 cut(s) 250, 1385
RsaI GTAC 2 cut(s) 41, 1552
RsaNI GTAC 2 cut(s) 40, 1551
RseI CAYNNNNRTG 3 cut(s) 32, 278, 1245
SacI GAGCTC 1 cut(s) 1404
SapI GCTCTTC 2 cut(s) 165, 1008
SaqAI TTAA 6 cut(s) 330, 674, 908, 968, 1284, 1361
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 3 cut(s) 250, 577, 1385
Sau96I GGNCC 3 cut(s) 270, 902, 1277
SbfI CCTGCAGG 1 cut(s) 1276
ScaI AGTACT 1 cut(s) 1552
SchI GAGTC 3 cut(s) 307, 368, 698
ScrFI CCNGG 6 cut(s) 303, 498, 503, 538, 1355, 1489
SdaI CCTGCAGG 1 cut(s) 1276
SduI GDGCHC 5 cut(s) 177, 1150, 1270, 1404, 1560
SfaNI GCATC 9 cut(s) 321, 769, 878, 1078, 1129, 1163, 1203, 1516, 1580
SfcI CTRYAG 1 cut(s) 1272
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 2 cut(s) 902, 1277
SmiMI CAYNNNNRTG 3 cut(s) 32, 278, 1245
Sse8387I CCTGCAGG 1 cut(s) 1276
Sse9I AATT 5 cut(s) 11, 327, 737, 1412, 1494
SsiI CCGC 4 cut(s) 4, 50, 814, 944
SspI AATATT 1 cut(s) 678
SspMI CTAG 5 cut(s) 255, 575, 1232, 1389, 1605
SstI GAGCTC 1 cut(s) 1404
StyD4I CCNGG 6 cut(s) 301, 496, 501, 536, 1353, 1487
StyI CCWWGG 1 cut(s) 1073
TaaI ACNGT 4 cut(s) 44, 149, 544, 808
TaiI ACGT 1 cut(s) 1591
TaqI TCGA 3 cut(s) 349, 845, 1404
TasI AATT 5 cut(s) 11, 327, 737, 1412, 1494
TatI WGTACW 2 cut(s) 39, 1550
TfiI GAWTC 4 cut(s) 346, 763, 842, 1010
Tru1I TTAA 6 cut(s) 330, 674, 908, 968, 1284, 1361
Tru9I TTAA 6 cut(s) 330, 674, 908, 968, 1284, 1361
TscAI CASTG 3 cut(s) 400, 811, 1002
TseFI GTSAC 3 cut(s) 1044, 1333, 1444
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 3 cut(s) 1044, 1333, 1444
TspDTI ATGAA 4 cut(s) 449, 820, 1179, 1451
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 3 cut(s) 400, 811, 1002
VpaK11BI GGWCC 2 cut(s) 902, 1277
VspI ATTAAT 2 cut(s) 330, 674
XapI RAATTY 2 cut(s) 737, 1412
XbaI TCTAGA 2 cut(s) 1388, 1604
XmiI GTMKAC 1 cut(s) 532
XmnI GAANNNNTTC 2 cut(s) 113, 198
XspI CTAG 5 cut(s) 255, 575, 1232, 1389, 1605
ZrmI AGTACT 1 cut(s) 1552
Zsp2I ATGCAT 2 cut(s) 1156, 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.