MD03G1132400.v1.1

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008659
Physical Location & Seq
Forward (+)
13173452 .. 13177606
4155 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1132400.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGGCTCCACCTGGGAAGGTTTCTGAGTTTTGCAGCGAAGGCAATGATTGGTTTTGCAAAGCGCGATTGCTGAGTGATATAACTGTCGTGGTGGATGGCGTGAACTTCCACCTTCACAAGTTTCCTCTCATATCAAAATGTGGGAAGATATCACATATGTATGAAGAGTTCGAAAGCACAAGCAGGAGCATTTTCACTGCAGTTCTGGAGGAACTTCCTGGTGGCCCTGACATGTTCCTGATTGCAGCTAAATTCTGCTATGGTATGCGAGTGGAATTGACTCCAAGAAATGTAGTGATGGTGTACTGTGCAGCGGACTACCTTGAAATGACAGATGAGTATGGAGAAGGAAACTTGCTCTCAAAGTCGGAGAGTTTCTTCCATAACAATGTACTTCACAACTGGAAAGACTCTATCTTGGCTCTTCAAAGTTGCGAGCCTGTTATCCACTGGGCAGAAAAGCTTCACATAGTAAACAAGTGTATAAATGCTATATCAGTGATGGTTTGTACAGATCCTAGTTTGTTTGGATGGCCCATGATGATGTATGGTAGCTTACAGAGTCCTGGTGGAAGCATCCTGTGGAACGGAATTAACACCGGAGCGAGAATTCGAAGCTCTGAGTCTGACTGGTGGTTTGAAGACATCTCATACCTCAGTGTGAGTTTGTTTGAGAGGCTTATGAAGGCAATGGAAACAAGGGGCATTAGACCTGAAAACCTAGTGGGTGCTGTAATGCACTATGCCAGAAAGTACCTACCTGGCCTAGGACGATGGCAGGGTGGACAACATGGTATACCCAGAACAGTTACAAGTTTCAGTTTGACACCCATTGCCCTTGATCAAAAGGTAGTTTTAGAAAGCATTGAAAAACTTCTTCCCCGAAAGAAGGGGAAATCGTTTTGCCGTTTTCTACTTGGTCTTCTTCGTGTTGCATTAATATTGGGTGTCAGTCAAACCTGCAAGGATGCTTTGGAGAGGAGAATAGGGATGCAGCTGGAATTGGCAACCCTAGATGGTCTTCTAATTCCTACTTATTCAGATGCTGACGCATTGTACGATACTGACTGTGTTGAACGGATAATCCATCATTTTATGTCGTCAGAATCAAAGGTTACACCACTTTCTCTGTCATCTGTCAACATGGAAACATCACCTTCGTCAAGACCTTTACAGAAAGTTGCGAAGTTGGTAGATAGCTATATTGCGGAGGTTGCTTCAGATGTGACTTTAAAACCAGGAAAGATACGCTCTCTTGCAGAGGCCCTCCCGGAATCTTCAAGAACTTTATATGATGGGCTGTATAGGTCGCTGGATATATACTTCAAGGCACACCCTTGGCTCACAGATAAAGAGAAGGAAGATCTCTGCAGCATCATCGACTTCCAGAAGCTATCTATTGATGCCTGTTCCCATGCATCCCAAAATGAAAGGTTGCCACTCAGAGTTGTTCTTCAAGTACTGTTCTTTGAGCAGATGCATTTGAGAACGGCTCTAGCTGGTTGTCTTCATGGCTTGGAAACTCATAGTGGCCCTGCAGGTCCTACGACTGTCCAAGGTGACATGACTGGCCAGATTATACAGAGGGATGGGTGGGTGACTATGGTGCGTGAGAACCGGGTTCTAAAGGTGGATATGGAAAAGATGAGATCTAGAGTCGGTGAACTCGAAGAAGAGTTCAGTCAAATAAAGCAAGAGATGAAAAAGGTGAGCAAATCACACAGCTCCCTCAGTTCTCCTCGTGTGGTTGGCAGGAGAATTGGGTGCAGTCTTCTTTCACAACCATCTGATGCTAAACCAGATGCTATTCAAAGTACGCGGCCCTCCCCAAGATCATCAGTTGAGCAGGGACGCCCTTCGACCTTGCATATTAGACACAAAAAAAGTTCCTCGTTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

634

Amino Acids

70.92

Weight (kDa)

7.57

Isoelectric Point (pI)

48.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 210 - 470 1.5e-87 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 968, 1529
AccI GTMKAC 1 cut(s) 796
AccII CGCG 2 cut(s) 64, 1819
AciI CCGC 3 cut(s) 314, 1208, 1819
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 1570
AcsI RAATTY 2 cut(s) 251, 609
AcuI CTGAAG 1 cut(s) 1203
AcyI GRCGYC 1 cut(s) 1852
AfaI GTAC 7 cut(s) 305, 393, 511, 755, 1058, 1461, 1816
AfiI CCNNNNNNNGG 2 cut(s) 767, 889
AflIII ACRYGT 1 cut(s) 231
AgsI TTSAA 9 cut(s) 326, 428, 641, 869, 1076, 1281, 1327, 1457, 1811
AjnI CCWGG 5 cut(s) 10, 217, 565, 760, 1237
AloI GAACNNNNNNTCC 2 cut(s) 1068, 1100
AluBI AGCT 9 cut(s) 248, 463, 555, 618, 997, 1200, 1393, 1499, 1725
AluI AGCT 9 cut(s) 248, 463, 555, 618, 997, 1200, 1393, 1499, 1725
AlwI GGATC 1 cut(s) 509
AlwNI CAGNNNCTG 1 cut(s) 1046
AoxI GGCC 7 cut(s) 223, 533, 763, 1263, 1531, 1570, 1820
ApeKI GCWGC 5 cut(s) 33, 245, 311, 994, 1371
ApoI RAATTY 2 cut(s) 251, 609
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AseI ATTAAT 1 cut(s) 938
Asp700I GAANNNNTTC 2 cut(s) 462, 873
AspA2I CCTAGG 1 cut(s) 766
AspLEI GCGC 1 cut(s) 64
AspS9I GGNCC 6 cut(s) 224, 534, 1264, 1532, 1541, 1821
AsuC2I CCSGG 2 cut(s) 1271, 1619
AsuHPI GGTGA 5 cut(s) 1146, 1571, 1609, 1673, 1720
AsuII TTCGAA 2 cut(s) 171, 613
AvaII GGWCC 1 cut(s) 1541
AvrII CCTAGG 1 cut(s) 766
BalI TGGCCA 1 cut(s) 1572
BauI CACGAG 1 cut(s) 1740
BbsI GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BbvI GCAGC 5 cut(s) 45, 257, 323, 1006, 1383
BceAI ACGGC 2 cut(s) 891, 1506
BcgI CGANNNNNNTGC 2 cut(s) 1360, 1394
BciT130I CCWGG 5 cut(s) 12, 219, 567, 762, 1239
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 2 cut(s) 1271, 1619
BfaI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
BfmI CTRYAG 3 cut(s) 198, 1369, 1536
BfuAI ACCTGC 2 cut(s) 968, 1529
BglII AGATCT 2 cut(s) 1363, 1649
BisI GCNGC 6 cut(s) 34, 246, 312, 995, 1372, 1820
BlnI CCTAGG 1 cut(s) 766
BlsI GCNGC 6 cut(s) 35, 247, 313, 996, 1373, 1821
BmcAI AGTACT 1 cut(s) 1461
Bme1390I CCNGG 7 cut(s) 12, 219, 567, 762, 1239, 1271, 1619
Bme18I GGWCC 1 cut(s) 1541
BmgT120I GGNCC 6 cut(s) 224, 534, 1264, 1532, 1541, 1821
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 7 cut(s) 12, 219, 567, 762, 1239, 1271, 1619
BmrI ACTGGG 1 cut(s) 460
BmuI ACTGGG 1 cut(s) 460
BpiI GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BplI GAGNNNNNCTC 2 cut(s) 1477, 1509
BpmI CTGGAG 1 cut(s) 227
Bpu14I TTCGAA 2 cut(s) 171, 613
BpuMI CCSGG 2 cut(s) 1271, 1619
BsaHI GRCGYC 1 cut(s) 1852
BsaJI CCNNGG 4 cut(s) 11, 766, 1337, 1555
BsaWI WCCGGW 1 cut(s) 599
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 889
Bse1I ACTGG 4 cut(s) 407, 455, 635, 1573
Bse3DI GCAATG 3 cut(s) 49, 696, 831
BseBI CCWGG 5 cut(s) 12, 219, 567, 762, 1239
BseDI CCNNGG 4 cut(s) 11, 766, 1337, 1555
BseGI GGATG 7 cut(s) 100, 536, 576, 973, 996, 1418, 1594
BseLI CCNNNNNNNGG 2 cut(s) 767, 889
BseMI GCAATG 3 cut(s) 49, 696, 831
BseMII CTCAG 6 cut(s) 15, 62, 612, 670, 1456, 1744
BseNI ACTGG 4 cut(s) 407, 455, 635, 1573
BseRI GAGGAG 2 cut(s) 994, 1728
BseXI GCAGC 5 cut(s) 45, 257, 323, 1006, 1383
BsgI GTGCAG 2 cut(s) 330, 1786
Bsh1236I CGCG 2 cut(s) 64, 1819
BshFI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BsiSI CCGG 3 cut(s) 600, 1271, 1618
BslFI GGGAC 1 cut(s) 1863
BslI CCNNNNNNNGG 2 cut(s) 767, 889
BsmFI GGGAC 1 cut(s) 1863
BsnI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
Bsp119I TTCGAA 2 cut(s) 171, 613
Bsp1407I TGTACA 1 cut(s) 509
Bsp143I GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BspACI CCGC 3 cut(s) 314, 1208, 1819
BspANI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BspCNI CTCAG 6 cut(s) 16, 63, 613, 669, 1455, 1743
BspFNI CGCG 2 cut(s) 64, 1819
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 3 cut(s) 202, 1373, 1540
BspMI ACCTGC 2 cut(s) 968, 1529
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 429
BspT104I TTCGAA 2 cut(s) 171, 613
BsrDI GCAATG 3 cut(s) 49, 696, 831
BsrGI TGTACA 1 cut(s) 509
BsrI ACTGG 4 cut(s) 407, 455, 635, 1573
BssECI CCNNGG 4 cut(s) 11, 766, 1337, 1555
BssMI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BssNAI GTATAC 1 cut(s) 797
BssNI GRCGYC 1 cut(s) 1852
BssSI CACGAG 1 cut(s) 1740
BssT1I CCWWGG 3 cut(s) 766, 1337, 1555
Bst1107I GTATAC 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 1740
Bst2UI CCWGG 5 cut(s) 12, 219, 567, 762, 1239
Bst4CI ACNGT 6 cut(s) 85, 308, 808, 1070, 1464, 1552
Bst6I CTCTTC 3 cut(s) 159, 429, 1668
BstACI GRCGYC 1 cut(s) 1852
BstAUI TGTACA 1 cut(s) 509
BstBI TTCGAA 2 cut(s) 171, 613
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 6 cut(s) 24, 71, 621, 656, 1442, 1730
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 7 cut(s) 100, 536, 576, 973, 996, 1418, 1594
BstFNI CGCG 2 cut(s) 64, 1819
BstHHI GCGC 1 cut(s) 64
BstKTI GATC 5 cut(s) 517, 844, 1366, 1652, 1835
BstMBI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
BstMWI GCNNNNNNNGC 2 cut(s) 39, 1214
BstNI CCWGG 5 cut(s) 12, 219, 567, 762, 1239
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 7 cut(s) 10, 217, 565, 760, 1237, 1269, 1617
BstSFI CTRYAG 3 cut(s) 198, 1369, 1536
BstUI CGCG 2 cut(s) 64, 1819
BstV1I GCAGC 5 cut(s) 45, 257, 323, 1006, 1383
BstV2I GAAGAC 5 cut(s) 648, 914, 1013, 1499, 1763
BstX2I RGATCY 3 cut(s) 514, 1363, 1649
BstYI RGATCY 3 cut(s) 514, 1363, 1649
BstZ17I GTATAC 1 cut(s) 797
BsuRI GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
BtsCI GGATG 7 cut(s) 100, 536, 576, 973, 996, 1418, 1594
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 4 cut(s) 195, 448, 504, 664
BveI ACCTGC 2 cut(s) 968, 1529
Cac8I GCNNGC 1 cut(s) 437
CaiI CAGNNNCTG 1 cut(s) 1046
CfoI GCGC 1 cut(s) 64
Cfr13I GGNCC 6 cut(s) 224, 534, 1264, 1532, 1541, 1821
CseI GACGC 2 cut(s) 1058, 1860
Csp6I GTAC 7 cut(s) 304, 392, 510, 754, 1057, 1460, 1815
CviAII CATG 7 cut(s) 232, 538, 791, 1144, 1415, 1511, 1564
CviQI GTAC 7 cut(s) 304, 392, 510, 754, 1057, 1460, 1815
DdeI CTNAG 6 cut(s) 24, 71, 621, 656, 1442, 1730
DpnI GATC 5 cut(s) 516, 843, 1365, 1651, 1834
DpnII GATC 5 cut(s) 514, 841, 1363, 1649, 1832
DraI TTTAAA 1 cut(s) 1233
EaeI YGGCCR 1 cut(s) 1570
Eam1104I CTCTTC 3 cut(s) 159, 429, 1668
EarI CTCTTC 3 cut(s) 159, 429, 1668
Eco130I CCWWGG 3 cut(s) 766, 1337, 1555
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 1541
Eco57I CTGAAG 1 cut(s) 1203
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoO109I RGGNCCY 2 cut(s) 1264, 1541
EcoRI GAATTC 1 cut(s) 609
EcoRII CCWGG 5 cut(s) 10, 217, 565, 760, 1237
EcoRV GATATC 1 cut(s) 150
EcoT14I CCWWGG 3 cut(s) 766, 1337, 1555
EcoT22I ATGCAT 2 cut(s) 1420, 1482
ErhI CCWWGG 3 cut(s) 766, 1337, 1555
FaeI CATG 7 cut(s) 235, 541, 794, 1147, 1418, 1514, 1567
FaqI GGGAC 1 cut(s) 1863
FatI CATG 7 cut(s) 231, 537, 790, 1143, 1414, 1510, 1563
FauNDI CATATG 1 cut(s) 156
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 796
Fnu4HI GCNGC 6 cut(s) 34, 246, 312, 995, 1372, 1820
FokI GGATG 7 cut(s) 107, 543, 563, 980, 1003, 1405, 1601
Fsp4HI GCNGC 6 cut(s) 34, 246, 312, 995, 1372, 1820
FspBI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
GlaI GCGC 1 cut(s) 63
GluI GCNGC 6 cut(s) 34, 246, 312, 995, 1372, 1820
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 7 cut(s) 225, 535, 765, 1265, 1533, 1572, 1822
HapII CCGG 3 cut(s) 600, 1271, 1618
HgaI GACGC 2 cut(s) 1058, 1860
HhaI GCGC 1 cut(s) 64
Hin1I GRCGYC 1 cut(s) 1852
Hin1II CATG 7 cut(s) 235, 541, 794, 1147, 1418, 1514, 1567
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 7 cut(s) 280, 410, 562, 623, 1106, 1274, 1656
HpaII CCGG 3 cut(s) 600, 1271, 1618
HphI GGTGA 5 cut(s) 1146, 1571, 1609, 1673, 1720
Hpy166II GTNNAC 7 cut(s) 103, 304, 475, 785, 797, 1141, 1664
Hpy188I TCNGA 9 cut(s) 25, 370, 622, 628, 1042, 1105, 1222, 1445, 1789
Hpy188III TCNNGA 6 cut(s) 206, 238, 1164, 1281, 1387, 1653
Hpy8I GTNNAC 7 cut(s) 103, 304, 475, 785, 797, 1141, 1664
HpyAV CCTTC 9 cut(s) 10, 32, 122, 341, 679, 883, 1167, 1351, 1866
HpyCH4III ACNGT 6 cut(s) 85, 308, 808, 1070, 1464, 1552
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 1214
HpyF3I CTNAG 6 cut(s) 24, 71, 621, 656, 1442, 1730
Hsp92I GRCGYC 1 cut(s) 1852
Hsp92II CATG 7 cut(s) 235, 541, 794, 1147, 1418, 1514, 1567
HspAI GCGC 1 cut(s) 62
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 5 cut(s) 514, 841, 1363, 1649, 1832
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 4 cut(s) 10, 186, 602, 1730
Lsp1109I GCAGC 5 cut(s) 45, 257, 323, 1006, 1383
MaeI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
MaeIII GTNAC 5 cut(s) 808, 1114, 1225, 1559, 1597
MalI GATC 5 cut(s) 516, 843, 1365, 1651, 1834
MboI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
MflI RGATCY 3 cut(s) 514, 1363, 1649
MlsI TGGCCA 1 cut(s) 1572
MluCI AATT 7 cut(s) 251, 275, 591, 609, 1001, 1026, 1758
MluNI TGGCCA 1 cut(s) 1572
MlyI GAGTC 5 cut(s) 274, 404, 571, 632, 1665
MmeI TCCRAC 1 cut(s) 348
Mox20I TGGCCA 1 cut(s) 1572
Mph1103I ATGCAT 2 cut(s) 1420, 1482
MroXI GAANNNNTTC 2 cut(s) 462, 873
MscI TGGCCA 1 cut(s) 1572
MseI TTAA 3 cut(s) 594, 938, 1232
MslI CAYNNNNRTG 3 cut(s) 159, 387, 542
Msp20I TGGCCA 1 cut(s) 1572
MspA1I CMGCKG 2 cut(s) 314, 997
MspI CCGG 3 cut(s) 600, 1271, 1618
MspR9I CCNGG 7 cut(s) 12, 219, 567, 762, 1239, 1271, 1619
MvaI CCWGG 5 cut(s) 12, 219, 567, 762, 1239
MvnI CGCG 2 cut(s) 64, 1819
MwoI GCNNNNNNNGC 2 cut(s) 39, 1214
NciI CCSGG 2 cut(s) 1271, 1619
NdeI CATATG 1 cut(s) 156
NdeII GATC 5 cut(s) 514, 841, 1363, 1649, 1832
NlaIII CATG 7 cut(s) 235, 541, 794, 1147, 1418, 1514, 1567
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 1225, 1559, 1597
NsiI ATGCAT 2 cut(s) 1420, 1482
NspI RCATGY 1 cut(s) 235
NspV TTCGAA 2 cut(s) 171, 613
PciI ACATGT 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 2 cut(s) 1056, 1665
PdmI GAANNNNTTC 2 cut(s) 462, 873
PfeI GAWTC 2 cut(s) 1106, 1274
PfoI TCCNGGA 1 cut(s) 1269
PkrI GCNGC 6 cut(s) 35, 247, 313, 996, 1373, 1821
PleI GAGTC 5 cut(s) 274, 404, 570, 631, 1664
PpsI GAGTC 5 cut(s) 274, 404, 570, 631, 1664
PpuMI RGGWCCY 1 cut(s) 1541
PscI ACATGT 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 938
Psp5II RGGWCCY 1 cut(s) 1541
Psp6I CCWGG 5 cut(s) 10, 217, 565, 760, 1237
PspGI CCWGG 5 cut(s) 10, 217, 565, 760, 1237
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 6 cut(s) 224, 534, 1264, 1532, 1541, 1821
PspPPI RGGWCCY 1 cut(s) 1541
PsrI GAACNNNNNNTAC 2 cut(s) 152, 184
PstI CTGCAG 3 cut(s) 202, 1373, 1540
PstNI CAGNNNCTG 1 cut(s) 1046
PsuI RGATCY 3 cut(s) 514, 1363, 1649
PvuII CAGCTG 1 cut(s) 997
RsaI GTAC 7 cut(s) 305, 393, 511, 755, 1058, 1461, 1816
RsaNI GTAC 7 cut(s) 304, 392, 510, 754, 1057, 1460, 1815
RseI CAYNNNNRTG 3 cut(s) 159, 387, 542
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 3 cut(s) 594, 938, 1232
SatI GCNGC 6 cut(s) 34, 246, 312, 995, 1372, 1820
Sau3AI GATC 5 cut(s) 514, 841, 1363, 1649, 1832
Sau96I GGNCC 6 cut(s) 224, 534, 1264, 1532, 1541, 1821
SbfI CCTGCAGG 1 cut(s) 1540
ScaI AGTACT 1 cut(s) 1461
SchI GAGTC 5 cut(s) 274, 404, 571, 632, 1665
ScrFI CCNGG 7 cut(s) 12, 219, 567, 762, 1239, 1271, 1619
SdaI CCTGCAGG 1 cut(s) 1540
SfcI CTRYAG 3 cut(s) 198, 1369, 1536
SfuI TTCGAA 2 cut(s) 171, 613
SinI GGWCC 1 cut(s) 1541
SmiMI CAYNNNNRTG 3 cut(s) 159, 387, 542
Sse8387I CCTGCAGG 1 cut(s) 1540
Sse9I AATT 7 cut(s) 251, 275, 591, 609, 1001, 1026, 1758
SsiI CCGC 3 cut(s) 314, 1208, 1819
SspI AATATT 1 cut(s) 942
SspMI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
StyD4I CCNGG 7 cut(s) 10, 217, 565, 760, 1237, 1269, 1617
StyI CCWWGG 3 cut(s) 766, 1337, 1555
TaaI ACNGT 6 cut(s) 85, 308, 808, 1070, 1464, 1552
TaqI TCGA 5 cut(s) 171, 613, 1380, 1668, 1859
TasI AATT 7 cut(s) 251, 275, 591, 609, 1001, 1026, 1758
TatI WGTACW 4 cut(s) 303, 391, 509, 1459
TauI GCSGC 1 cut(s) 1822
TfiI GAWTC 2 cut(s) 1106, 1274
Tru1I TTAA 3 cut(s) 594, 938, 1232
Tru9I TTAA 3 cut(s) 594, 938, 1232
TscAI CASTG 4 cut(s) 202, 455, 504, 664
TseFI GTSAC 3 cut(s) 1225, 1559, 1597
TseI GCWGC 5 cut(s) 33, 245, 311, 994, 1371
Tsp45I GTSAC 3 cut(s) 1225, 1559, 1597
TspDTI ATGAA 5 cut(s) 177, 698, 1443, 1499, 1715
TspGWI ACGGA 2 cut(s) 603, 1093
TspRI CASTG 4 cut(s) 202, 455, 504, 664
VpaK11BI GGWCC 1 cut(s) 1541
VspI ATTAAT 1 cut(s) 938
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 2 cut(s) 251, 609
XbaI TCTAGA 1 cut(s) 1652
XceI RCATGY 1 cut(s) 235
XmaJI CCTAGG 1 cut(s) 766
XmiI GTMKAC 1 cut(s) 796
XmnI GAANNNNTTC 2 cut(s) 462, 873
XspI CTAG 6 cut(s) 519, 722, 767, 1013, 1496, 1653
ZrmI AGTACT 1 cut(s) 1461
Zsp2I ATGCAT 2 cut(s) 1420, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.