FvH4_3g30910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008709
Physical Location & Seq
Reverse (-)
24481075 .. 24486940
5866 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30910.t5

Sequence Viewer

Length: 501 bp
ATGAGTCTCCGGCAGCGAGTGATGGAAGCCAAAGAGCCAACGACGATGATGTGCACACTTGTAGCAATAGACCACACTGACTTCTCCTACAGAGCATGCTCCCTCTGCGAGAGGACTCTCCCTGACAACCCATCTTCCCTTTGCAGATACTGCAAACCCTCCGCTTTCAACCCCCGCTTTCCTCGCACTAAACGCTTCTTTCGCGTTCTCATGTCAGTTGCTTCGGATAGCAAGGTGTTCACTGTGATATGCTTTGATAGGGTGGCCAAGGTCCTCTTTGGTTGCTCTGCTGATGAGTTTTTCGACTTTGCGAAATTTCACCCGTTTGCTGCAACTAATGCTAGTAGAATTTTGGAGGGAGAGATGTTTAAGATGGCAATATCCAAACCGAAGAATGGTAATGCACAAAATCTGCGAGCAGTTCAAGTTGTACCATTGAGAACTGGTTTTCAGCCGGCAATTGTGACCTTAAGGGAATTGTATGGCGTAAGAAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rep_fac-A_C

Protein Analysis

167

Amino Acids

18.8

Weight (kDa)

9.59

Isoelectric Point (pI)

36.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_C PF08646 15 - 103 2.9e-06 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 204
AciI CCGC 2 cut(s) 162, 175
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 2 cut(s) 314, 348
AfaI GTAC 1 cut(s) 432
AfiI CCNNNNNNNGG 1 cut(s) 395
AflII CTTAAG 1 cut(s) 469
AgsI TTSAA 2 cut(s) 169, 425
AluBI AGCT 1 cut(s) 495
AluI AGCT 1 cut(s) 495
Alw21I GWGCWC 1 cut(s) 56
Alw26I GTCTC 1 cut(s) 11
Alw44I GTGCAC 1 cut(s) 52
AlwNI CAGNNNCTG 1 cut(s) 150
AoxI GGCC 1 cut(s) 264
ApaLI GTGCAC 1 cut(s) 52
ApeKI GCWGC 2 cut(s) 13, 329
ApoI RAATTY 2 cut(s) 314, 348
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 311
AvaII GGWCC 1 cut(s) 271
BaeGI GKGCMC 1 cut(s) 56
BalI TGGCCA 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 25, 316
BccI CCATC 3 cut(s) 16, 139, 367
BcoDI GTCTC 1 cut(s) 11
BfaI CTAG 1 cut(s) 342
BfmI CTRYAG 1 cut(s) 88
BfrI CTTAAG 1 cut(s) 469
BisI GCNGC 2 cut(s) 14, 330
BlsI GCNGC 2 cut(s) 15, 331
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 454
Bse1I ACTGG 1 cut(s) 448
BseDI CCNNGG 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 395
BseNI ACTGG 1 cut(s) 448
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 25, 316
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 56
BsiSI CCGG 2 cut(s) 10, 455
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 11
BsnI GGCC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 56
BspACI CCGC 2 cut(s) 162, 175
BspANI GGCC 1 cut(s) 266
BspFNI CGCG 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 454
BsrI ACTGG 1 cut(s) 448
BssAI RCCGGY 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 244
BstAFI CTTAAG 1 cut(s) 469
BstAPI GCANNNNNTGC 2 cut(s) 150, 338
BstC8I GCNNGC 3 cut(s) 97, 417, 456
BstFNI CGCG 1 cut(s) 204
BstMAI GTCTC 1 cut(s) 11
BstMWI GCNNNNNNNGC 7 cut(s) 105, 150, 183, 192, 201, 338, 492
BstNSI RCATGY 1 cut(s) 99
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 25, 316
BsuRI GGCC 1 cut(s) 266
BtsIMutI CAGTG 2 cut(s) 75, 240
Cac8I GCNNGC 3 cut(s) 97, 417, 456
CaiI CAGNNNCTG 1 cut(s) 150
Cfr10I RCCGGY 1 cut(s) 454
Cfr13I GGNCC 1 cut(s) 271
Csp6I GTAC 1 cut(s) 431
CviAII CATG 2 cut(s) 96, 211
CviJI RGCY 5 cut(s) 29, 37, 266, 454, 495
CviKI_1 RGCY 5 cut(s) 29, 37, 266, 454, 495
CviQI GTAC 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 264
Eco130I CCWWGG 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 271
EcoO109I RGGNCCY 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 2 cut(s) 99, 214
FaiI YATR 4 cut(s) 97, 212, 250, 483
FalI AAGNNNNNCTT 3 cut(s) 260, 292, 481
FatI CATG 2 cut(s) 95, 210
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 2 cut(s) 14, 330
Fsp4HI GCNGC 2 cut(s) 14, 330
FspBI CTAG 1 cut(s) 342
GluI GCNGC 2 cut(s) 14, 330
HaeIII GGCC 1 cut(s) 266
HapII CCGG 2 cut(s) 10, 455
Hin1II CATG 2 cut(s) 99, 214
HinfI GANTC 2 cut(s) 4, 115
HpaII CCGG 2 cut(s) 10, 455
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 54, 240
Hpy188I TCNGA 1 cut(s) 226
Hpy188III TCNNGA 1 cut(s) 498
Hpy8I GTNNAC 2 cut(s) 54, 240
Hpy99I CGWCG 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 5 cut(s) 54, 144, 153, 332, 404
HpyF10VI GCNNNNNNNGC 7 cut(s) 105, 150, 183, 192, 201, 338, 492
Hsp92II CATG 2 cut(s) 99, 214
KroI GCCGGC 1 cut(s) 454
KroNI GCCGGC 1 cut(s) 456
LmnI GCTCC 1 cut(s) 104
LpnPI CCDG 4 cut(s) 23, 135, 429, 468
Lsp1109I GCAGC 2 cut(s) 25, 316
MaeI CTAG 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 463
MboII GAAGA 2 cut(s) 126, 403
MfeI CAATTG 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 56
MlsI TGGCCA 1 cut(s) 266
MluCI AATT 4 cut(s) 314, 348, 459, 476
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 2 cut(s) 13, 109
MnlI CCTC 6 cut(s) 105, 113, 169, 192, 284, 349
Mox20I TGGCCA 1 cut(s) 266
MroNI GCCGGC 1 cut(s) 454
MscI TGGCCA 1 cut(s) 266
MseI TTAA 2 cut(s) 369, 470
Msp20I TGGCCA 1 cut(s) 266
MspCI CTTAAG 1 cut(s) 469
MspI CCGG 2 cut(s) 10, 455
MunI CAATTG 1 cut(s) 459
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 7 cut(s) 105, 150, 183, 192, 201, 338, 492
NaeI GCCGGC 1 cut(s) 456
NgoMIV GCCGGC 1 cut(s) 454
NlaIII CATG 2 cut(s) 99, 214
NmuCI GTSAC 1 cut(s) 463
NspI RCATGY 1 cut(s) 99
PaeI GCATGC 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 456
PkrI GCNGC 2 cut(s) 15, 331
PleI GAGTC 2 cut(s) 12, 109
PpsI GAGTC 2 cut(s) 12, 109
PpuMI RGGWCCY 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 271
PspPI GGNCC 1 cut(s) 271
PspPPI RGGWCCY 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 150
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
SaqAI TTAA 2 cut(s) 369, 470
SatI GCNGC 2 cut(s) 14, 330
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 2 cut(s) 13, 109
SduI GDGCHC 1 cut(s) 56
SetI ASST 4 cut(s) 237, 273, 470, 497
SfcI CTRYAG 1 cut(s) 88
SinI GGWCC 1 cut(s) 271
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
SphI GCATGC 1 cut(s) 99
Sse9I AATT 4 cut(s) 314, 348, 459, 476
SsiI CCGC 2 cut(s) 162, 175
SspMI CTAG 1 cut(s) 342
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 1 cut(s) 303
TasI AATT 4 cut(s) 314, 348, 459, 476
Tru1I TTAA 2 cut(s) 369, 470
Tru9I TTAA 2 cut(s) 369, 470
TscAI CASTG 2 cut(s) 82, 247
TseFI GTSAC 1 cut(s) 463
TseI GCWGC 2 cut(s) 13, 329
Tsp45I GTSAC 1 cut(s) 463
TspRI CASTG 2 cut(s) 82, 247
Vha464I CTTAAG 1 cut(s) 469
VneI GTGCAC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 271
XapI RAATTY 2 cut(s) 314, 348
XceI RCATGY 1 cut(s) 99
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.