FvH4_3g31112

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0018007
Physical Location & Seq
Forward (+)
24887647 .. 24894480
6834 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31112.t1

Sequence Viewer

Length: 753 bp
ATGAAGAATGCTGGTTTCGTCGCCACTCCTGGTTTCTTATCTTCGAACAAAGTTGAATACAGAACACTATGGTTAAGAACTGTGGGAGTTAGGAATTATTGTGTCAAGGCTAGCTACTTGAGTGAGATTGACAAGAAACCAGGAGTGGCGACGAAACCAGCAGTCTTCGTCTCCGACGCGAAGACGCTCGTGAAAGCTTGCCGCTTGGAAGTGTCCTCCTCTCAGGCTGAGTTGCGTTGCGCTTCGTGGCCACCGAATCAGAGAGCGACTCCGAGAGGCGATGGAGGAAAAGACGAAGAGGAGGAGCTCAATTTGTGTTCTATTCCCACAGTGATTGGATCTTCTCCACAACAAATTACAATTCAGCATTCTATTGATGGCACTCTTACACAGTTCACCTTTGTGTATGCTGCCACAACTATGCGCTTGCGTAAGACTTTGTGGTCGGAGTTATTGAATCTCAGACAAAGTACTACGCTTTCGTGGATGGTTATTGGCGACTTTAATGCTGTGTTAGGAGCTCACGAGTCTTCTAGAGGAAGGACTCCTAATCAGTCATCTTGTGAAGACTTTCGGAGTGTCACAGAGCTTTGTAACTTCCAACATTTGGATACCTCTTGTGCGTCTTTTACACGGTCCAGAGGCTTAGGGCCTCGTCGAATAGAGCGTCGTCTTGACAGATCCTTATGTGATGAGACTTGGTTTTATTCTTGGCCGTACACAAGTTGTATTGCTTTCCCTCGAGTGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

251

Amino Acids

28.04

Weight (kDa)

9.0

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
AccB7I CCANNNNNTGG 1 cut(s) 607
AccII CGCG 1 cut(s) 179
AciI CCGC 1 cut(s) 202
AclWI GGATC 2 cut(s) 346, 675
AcoI YGGCCR 2 cut(s) 248, 713
AfaI GTAC 2 cut(s) 472, 719
AfiI CCNNNNNNNGG 2 cut(s) 607, 745
AgsI TTSAA 2 cut(s) 56, 457
AjnI CCWGG 2 cut(s) 28, 139
AleI CACNNNNGTG 1 cut(s) 401
AluBI AGCT 5 cut(s) 114, 197, 307, 521, 589
AluI AGCT 5 cut(s) 114, 197, 307, 521, 589
Alw21I GWGCWC 2 cut(s) 309, 523
Alw26I GTCTC 2 cut(s) 175, 689
AlwI GGATC 2 cut(s) 346, 675
Ama87I CYCGRG 1 cut(s) 741
AoxI GGCC 3 cut(s) 248, 650, 713
ApeKI GCWGC 1 cut(s) 410
ArsI GACNNNNNNTTYG 2 cut(s) 652, 684
Asp700I GAANNNNTTC 1 cut(s) 570
AspLEI GCGC 2 cut(s) 242, 426
AspS9I GGNCC 2 cut(s) 636, 650
AsuHPI GGTGA 1 cut(s) 388
AsuII TTCGAA 1 cut(s) 44
AsuNHI GCTAGC 1 cut(s) 110
AvaI CYCGRG 1 cut(s) 741
AvaII GGWCC 1 cut(s) 636
BalI TGGCCA 1 cut(s) 250
BanII GRGCYC 2 cut(s) 309, 523
BauI CACGAG 2 cut(s) 188, 524
BbsI GAAGAC 4 cut(s) 157, 188, 522, 573
Bbv12I GWGCWC 2 cut(s) 309, 523
BbvI GCAGC 1 cut(s) 397
BccI CCATC 3 cut(s) 275, 371, 481
BceAI ACGGC 1 cut(s) 700
BcgI CGANNNNNNTGC 2 cut(s) 488, 522
BciT130I CCWGG 2 cut(s) 30, 141
BciVI GTATCC 1 cut(s) 604
BcoDI GTCTC 2 cut(s) 175, 689
BfaI CTAG 2 cut(s) 111, 534
BfuI GTATCC 1 cut(s) 604
BisI GCNGC 2 cut(s) 202, 411
BlsI GCNGC 2 cut(s) 203, 412
BmcAI AGTACT 1 cut(s) 472
Bme1390I CCNGG 2 cut(s) 30, 141
Bme18I GGWCC 1 cut(s) 636
BmeT110I CYCGRG 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 636, 650
BmrFI CCNGG 2 cut(s) 30, 141
BmtI GCTAGC 1 cut(s) 114
BpiI GAAGAC 4 cut(s) 157, 188, 522, 573
BplI GAGNNNNNCTC 2 cut(s) 253, 285
Bpu10I CCTNAGC 1 cut(s) 646
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 139
Bsc4I CCNNNNNNNGG 2 cut(s) 607, 745
BseBI CCWGG 2 cut(s) 30, 141
BseGI GGATG 1 cut(s) 492
BseLI CCNNNNNNNGG 2 cut(s) 607, 745
BseMII CTCAG 3 cut(s) 219, 236, 475
BseRI GAGGAG 3 cut(s) 208, 314, 317
BseXI GCAGC 1 cut(s) 397
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 3 cut(s) 250, 652, 715
BsiHKAI GWGCWC 2 cut(s) 309, 523
BsiHKCI CYCGRG 1 cut(s) 741
BslI CCNNNNNNNGG 2 cut(s) 607, 745
BsmAI GTCTC 2 cut(s) 175, 689
BsmBI CGTCTC 1 cut(s) 175
BsmI GAATGC 2 cut(s) 13, 367
BsnI GGCC 3 cut(s) 250, 652, 715
BsoBI CYCGRG 1 cut(s) 741
Bsp119I TTCGAA 1 cut(s) 44
Bsp1286I GDGCHC 2 cut(s) 309, 523
Bsp143I GATC 2 cut(s) 338, 680
BspACI CCGC 1 cut(s) 202
BspANI GGCC 3 cut(s) 250, 652, 715
BspCNI CTCAG 3 cut(s) 220, 235, 474
BspFNI CGCG 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 114
BspPI GGATC 2 cut(s) 346, 675
BspT104I TTCGAA 1 cut(s) 44
BssMI GATC 2 cut(s) 338, 680
BssSI CACGAG 2 cut(s) 188, 524
Bst2BI CACGAG 2 cut(s) 188, 524
Bst2UI CCWGG 2 cut(s) 30, 141
Bst4CI ACNGT 4 cut(s) 82, 331, 393, 636
Bst6I CTCTTC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 112, 199, 428
BstDEI CTNAG 4 cut(s) 222, 228, 461, 646
BstF5I GGATG 1 cut(s) 492
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 2 cut(s) 242, 426
BstKTI GATC 2 cut(s) 341, 683
BstMAI GTCTC 2 cut(s) 175, 689
BstMBI GATC 2 cut(s) 338, 680
BstNI CCWGG 2 cut(s) 30, 141
BstSCI CCNGG 2 cut(s) 28, 139
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 4 cut(s) 157, 188, 522, 573
BstX2I RGATCY 2 cut(s) 338, 680
BstYI RGATCY 2 cut(s) 338, 680
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 3 cut(s) 250, 652, 715
BtgZI GCGATG 1 cut(s) 294
BtsCI GGATG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 336
Cac8I GCNNGC 3 cut(s) 112, 199, 428
CfoI GCGC 2 cut(s) 242, 426
Cfr13I GGNCC 2 cut(s) 636, 650
CseI GACGC 4 cut(s) 185, 193, 612, 656
Csp6I GTAC 2 cut(s) 471, 718
CspCI CAANNNNNGTGG 2 cut(s) 316, 351
CviQI GTAC 2 cut(s) 471, 718
DdeI CTNAG 4 cut(s) 222, 228, 461, 646
DpnI GATC 2 cut(s) 340, 682
DpnII GATC 2 cut(s) 338, 680
DrdI GACNNNNNNGTC 1 cut(s) 442
DseDI GACNNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 2 cut(s) 248, 713
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Ecl136II GAGCTC 2 cut(s) 307, 521
Eco24I GRGCYC 2 cut(s) 309, 523
Eco47I GGWCC 1 cut(s) 636
Eco53kI GAGCTC 2 cut(s) 307, 521
Eco88I CYCGRG 1 cut(s) 741
EcoICRI GAGCTC 2 cut(s) 307, 521
EcoO109I RGGNCCY 1 cut(s) 650
EcoRII CCWGG 2 cut(s) 28, 139
EcoT38I GRGCYC 2 cut(s) 309, 523
Esp3I CGTCTC 1 cut(s) 175
FaiI YATR 4 cut(s) 70, 408, 422, 688
Fnu4HI GCNGC 2 cut(s) 202, 411
FokI GGATG 1 cut(s) 499
FriOI GRGCYC 2 cut(s) 309, 523
Fsp4HI GCNGC 2 cut(s) 202, 411
FspBI CTAG 2 cut(s) 111, 534
GlaI GCGC 2 cut(s) 241, 425
GluI GCNGC 2 cut(s) 202, 411
HaeIII GGCC 3 cut(s) 250, 652, 715
HgaI GACGC 4 cut(s) 185, 193, 612, 656
HhaI GCGC 2 cut(s) 242, 426
Hin6I GCGC 2 cut(s) 240, 424
HinP1I GCGC 2 cut(s) 240, 424
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 5 cut(s) 256, 268, 457, 527, 544
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 2 cut(s) 396, 720
Hpy188I TCNGA 7 cut(s) 175, 261, 273, 448, 464, 576, 752
Hpy188III TCNNGA 5 cut(s) 190, 524, 534, 639, 674
Hpy8I GTNNAC 2 cut(s) 396, 720
Hpy99I CGWCG 5 cut(s) 23, 154, 179, 660, 672
HpyAV CCTTC 1 cut(s) 534
HpyCH4III ACNGT 4 cut(s) 82, 331, 393, 636
HpyF3I CTNAG 4 cut(s) 222, 228, 461, 646
HspAI GCGC 2 cut(s) 240, 424
Kzo9I GATC 2 cut(s) 338, 680
LmnI GCTCC 2 cut(s) 304, 518
LpnPI CCDG 7 cut(s) 15, 42, 126, 153, 171, 209, 652
Lsp1109I GCAGC 1 cut(s) 397
MaeI CTAG 2 cut(s) 111, 534
MaeIII GTNAC 2 cut(s) 580, 593
MalI GATC 2 cut(s) 340, 682
MboI GATC 2 cut(s) 338, 680
MboII GAAGA 8 cut(s) 16, 33, 157, 193, 308, 333, 522, 578
MflI RGATCY 2 cut(s) 338, 680
MhlI GDGCHC 2 cut(s) 309, 523
MlsI TGGCCA 1 cut(s) 250
MluCI AATT 4 cut(s) 94, 310, 354, 360
MluNI TGGCCA 1 cut(s) 250
MlyI GAGTC 3 cut(s) 262, 536, 538
MmeI TCCRAC 3 cut(s) 198, 426, 625
Mox20I TGGCCA 1 cut(s) 250
MroXI GAANNNNTTC 1 cut(s) 570
MscI TGGCCA 1 cut(s) 250
MseI TTAA 2 cut(s) 74, 504
MslI CAYNNNNRTG 2 cut(s) 401, 419
Msp20I TGGCCA 1 cut(s) 250
MspR9I CCNGG 2 cut(s) 30, 141
Mva1269I GAATGC 2 cut(s) 13, 367
MvaI CCWGG 2 cut(s) 30, 141
MvnI CGCG 1 cut(s) 179
NdeII GATC 2 cut(s) 338, 680
NheI GCTAGC 1 cut(s) 110
NmuCI GTSAC 1 cut(s) 580
NspV TTCGAA 1 cut(s) 44
OliI CACNNNNGTG 1 cut(s) 401
PaeR7I CTCGAG 1 cut(s) 741
PcsI WCGNNNNNNNCGW 2 cut(s) 251, 664
PctI GAATGC 2 cut(s) 13, 367
PdmI GAANNNNTTC 1 cut(s) 570
PfeI GAWTC 2 cut(s) 256, 457
PflMI CCANNNNNTGG 1 cut(s) 607
PkrI GCNGC 2 cut(s) 203, 412
PleI GAGTC 3 cut(s) 262, 535, 538
PpsI GAGTC 3 cut(s) 262, 535, 538
Psp124BI GAGCTC 2 cut(s) 309, 523
Psp6I CCWGG 2 cut(s) 28, 139
PspGI CCWGG 2 cut(s) 28, 139
PspPI GGNCC 2 cut(s) 636, 650
PspXI VCTCGAGB 1 cut(s) 741
PsuI RGATCY 2 cut(s) 338, 680
RsaI GTAC 2 cut(s) 472, 719
RsaNI GTAC 2 cut(s) 471, 718
RseI CAYNNNNRTG 2 cut(s) 401, 419
SacI GAGCTC 2 cut(s) 309, 523
SaqAI TTAA 2 cut(s) 74, 504
SatI GCNGC 2 cut(s) 202, 411
Sau3AI GATC 2 cut(s) 338, 680
Sau96I GGNCC 2 cut(s) 636, 650
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 3 cut(s) 262, 536, 538
ScrFI CCNGG 2 cut(s) 30, 141
SduI GDGCHC 2 cut(s) 309, 523
SetI ASST 7 cut(s) 116, 199, 309, 401, 523, 591, 617
Sfr274I CTCGAG 1 cut(s) 741
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 1 cut(s) 636
SlaI CTCGAG 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 401, 419
SmlI CTYRAG 2 cut(s) 118, 741
SmoI CTYRAG 2 cut(s) 118, 741
Sse9I AATT 4 cut(s) 94, 310, 354, 360
SsiI CCGC 1 cut(s) 202
SspMI CTAG 2 cut(s) 111, 534
SstI GAGCTC 2 cut(s) 309, 523
StyD4I CCNGG 2 cut(s) 28, 139
TaaI ACNGT 4 cut(s) 82, 331, 393, 636
TaqI TCGA 3 cut(s) 44, 658, 742
TasI AATT 4 cut(s) 94, 310, 354, 360
TatI WGTACW 1 cut(s) 470
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 2 cut(s) 256, 457
Tru1I TTAA 2 cut(s) 74, 504
Tru9I TTAA 2 cut(s) 74, 504
TscAI CASTG 1 cut(s) 336
TseFI GTSAC 1 cut(s) 580
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 580
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 336
Van91I CCANNNNNTGG 1 cut(s) 607
VpaK11BI GGWCC 1 cut(s) 636
XbaI TCTAGA 1 cut(s) 533
XhoI CTCGAG 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 570
XspI CTAG 2 cut(s) 111, 534
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.