FvH4_3g36150

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016534
Physical Location & Seq
Reverse (-)
30985217 .. 30987762
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g36150.t1

Sequence Viewer

Length: 1917 bp
ATGGAGGCCAGAACCCTTCTATCCGTTATACAAAGCTTTAACCCCTCTTTACACACATCATTTCCACAATATGAAAGTAACGCTTTTCGTCAAAAAAACGATAACATAAACCGAAAGCGACGAATCAAACAGAACACGGCAACACGCAAACACGACCCACTTCGCCACGTCCTACCTACTTCCGTCAACAAAAACGACCAAAAGCAACACGTAAACTCCCAATCAAGCCGAACTAGGCGGGTCCCACTACCCCCTTCCAGATCACACACACAAAACCTTTCATTTCGAAAACAACATATGCTCGCTTCCCAGGTTACCAGTAGTACCACACTACCACTCTTCTTTACTTTTCCTCTTTCTCAAAGAGCCATGGACCCCAACAAGCTCCTCCTCCTCTGCTTCCTCCTCTCCGCCTTCCACGTCATCCTCACCTCCGCCATCGGCGTCAACTACGGCACAGTAGCCAACAACCTCCCCCCTCCTTCCCAAGTCGCCACCTTCCTCAAGACCAAGACCACCATCGACCGCGTCAAGATCTTCAACACCGACCCCGCCGTCCTCCGTGCCTTCGCCAACACCGGCATCGCTGTCACCGTCACCGTCGGCAACGGCAACGTCCCCCCGCTCGCTAAGCTCCCAGCCGCCCAGTCCTGGGTCGCCGACAACATCCTCCCCTTCTACCCGCGCACCAACATCAACCGAATCGCCGTGGGAAACGAAATCCTCGCCACGTCGGACAAGGACCTCATCGCGCATCTCCTCCCGGCTATGAAGGCGCTCCACTCGGCTCTCAACCTGGCCAATGTCACCTCCATTCAGGTCTCGACACCGCACTCCCTCGGCATTCTCGCACCCAACACAGGCCCACCGAGCCTCGGACAGTTCCGCAGCGGCTACGACAAGGTCATTTTCCAGCCGATTTTGGAGTATCATCGCCAGACCAAATCGCCGTTCCTCGTCAACCCCTACCCGTTTTTCGGGCTCTCACCCGACACTTTAAACTACGCCACTTTCAGGCCAAACGCCGGCGTTTTCGACCCTGCCACCGGCGTTAACTACACCAACATGTTCGACGCCCAGATGGACGCGGTGTTCTCGGCCATGAAGAAGGTCGGGTACGAAGACGTGGATCTAGTAGTCGCCGAAACGGGTTGGCCCTCTGCCGGTGACCCGAACCAACTCGGTGTGAGCGTGGAAAACGCCGAGGCGTACAACGGGAACCTCATACGCCACGTCAGCTCCGGGAAGGGCACGCCGTTGATGCCGAACAGGACTTTCGAGACTTACATCTTCGCGCTGTTCAATGAGAACCTGAAGCCTACGTTGTCGGAGCAGAATTACGGGCTGTTCAAACCCGACCTCGACCCGGTTTACGACGTGGGGCTATTACGTAACGGGCAGGCAGAGGGGCCCACATCCGGCCCGGCACTGAGTCCGGCATCAGGCCCAGCAGCAGGCCCAGAAGCAATGGGTCCCGCGCCTGATGTGGTGGCGCAATCACCGGCAGCGGCGGGGAAGAGCTGGTGCGTGCCGAAGTCGAACGTTTCCGACGCGGCGCTGCAGAAGAACATAGACTACGTATGCAGCAGTGGAGTGGACTGCAAGCCCATAAAGCAAGGTGGGCCGTGCTTTGAGCCCAATTCAGTGAAGTCGCACGCGGCTTACGTCATGAACGCTTATTACCAGGAGCATGGCCCACAGAATATCAACTGCGATTTCAACGGCACCGGAGTCGTCACCTCTACTGATCCCAGTTACAAGACGTGCACGTACCCTACCAAAGTGGCGGAGAAGGAGGAAAAGTCCCCGGGAAGTTCCCCTGCGAAGTCGGTGGCGGGAAGCTCAACGAGGATGAGATGTAGCAAAGTGGCTCTGTTCTCACTAATGGTCGGTTGTGGGGTAATGTTTAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

639

Amino Acids

69.12

Weight (kDa)

9.22

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 147 - 460 1.6e-78 Glycosyl hydrolases family 17
X8 PF07983 507 - 577 4.1e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46570
fragaria_vesca FvH4_3g36150
malus_domestica MD11G1107600.v1.1
prunus_persica Prupe.6G076900_v2.0.a1
pyrus_communis pycom11g08910
rosa_chinensis RchiOBHm_Chr5g0064591 RchiOBHm_Chr5g0064901
rosa_laevigata RLG00000035739
rosa_roxburghii Rroxscaffold_1G00016410
rosa_rugosa Rorug05G0366600
rosa_samantha Rh5AG424700 Rh5BG439700 Rh5DG453000
rosa_wichuraiana Rw5G039930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 1724
AccBSI CCGCTC 1 cut(s) 625
AccII CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
AclI AACGTT 1 cut(s) 1542
AclWI GGATC 2 cut(s) 1137, 1742
AcoI YGGCCR 2 cut(s) 798, 1098
AcuI CTGAAG 1 cut(s) 1334
AcyI GRCGYC 2 cut(s) 444, 1074
AfaI GTAC 4 cut(s) 325, 1118, 1211, 1772
AflIII ACRYGT 2 cut(s) 208, 1065
AgsI TTSAA 4 cut(s) 541, 1303, 1351, 1720
AjiI CACGTC 7 cut(s) 169, 421, 732, 1126, 1234, 1378, 1764
AjnI CCWGG 4 cut(s) 309, 650, 795, 1683
AjuI GAANNNNNNNTTGG 2 cut(s) 935, 967
AloI GAACNNNNNNTCC 2 cut(s) 1076, 1108
AluBI AGCT 6 cut(s) 36, 385, 634, 1239, 1521, 1842
AluI AGCT 6 cut(s) 36, 385, 634, 1239, 1521, 1842
Alw21I GWGCWC 1 cut(s) 1769
Alw26I GTCTC 2 cut(s) 826, 1274
Alw44I GTGCAC 1 cut(s) 1765
AlwI GGATC 2 cut(s) 1137, 1742
Ama87I CYCGRG 1 cut(s) 1807
ApaI GGGCCC 1 cut(s) 1413
ApaLI GTGCAC 1 cut(s) 1765
ApeKI GCWGC 5 cut(s) 888, 1451, 1505, 1558, 1584
AspLEI GCGC 7 cut(s) 687, 754, 778, 1297, 1480, 1495, 1558
AsuC2I CCSGG 6 cut(s) 764, 1243, 1367, 1424, 1808, 1809
AsuHPI GGTGA 8 cut(s) 421, 583, 589, 799, 978, 1178, 1491, 1729
AsuII TTCGAA 1 cut(s) 286
AvaI CYCGRG 1 cut(s) 1807
AvaII GGWCC 4 cut(s) 241, 373, 742, 1472
BaeGI GKGCMC 3 cut(s) 1253, 1413, 1769
BalI TGGCCA 1 cut(s) 800
BanI GGYRCC 1 cut(s) 1724
BanII GRGCYC 3 cut(s) 984, 1413, 1638
BarI GAAGNNNNNNTAC 2 cut(s) 1100, 1132
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 1 cut(s) 1769
BbvI GCAGC 5 cut(s) 900, 1463, 1517, 1545, 1596
BccI CCATC 3 cut(s) 446, 527, 1075
BceAI ACGGC 9 cut(s) 153, 469, 539, 625, 692, 934, 1240, 1609, 1738
BcgI CGANNNNNNTGC 2 cut(s) 1714, 1748
BciT130I CCWGG 4 cut(s) 311, 652, 797, 1685
BcnI CCSGG 6 cut(s) 764, 1243, 1367, 1424, 1808, 1809
BcoDI GTCTC 2 cut(s) 826, 1274
BfaI CTAG 2 cut(s) 234, 1133
BfmI CTRYAG 1 cut(s) 1559
BfoI RGCGCY 2 cut(s) 779, 1559
BglII AGATCT 1 cut(s) 534
BlpI GCTNAGC 1 cut(s) 630
Bme18I GGWCC 4 cut(s) 241, 373, 742, 1472
BmeT110I CYCGRG 1 cut(s) 1807
BmgBI CACGTC 7 cut(s) 169, 421, 732, 1126, 1234, 1378, 1764
BmiI GGNNCC 9 cut(s) 242, 243, 375, 1220, 1410, 1411, 1473, 1474, 1726
BmrI ACTGGG 2 cut(s) 640, 1746
BmsI GCATC 4 cut(s) 591, 763, 1251, 1448
BmuI ACTGGG 2 cut(s) 640, 1746
BpiI GAAGAC 1 cut(s) 1128
Bpu1102I GCTNAGC 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 6 cut(s) 764, 1243, 1367, 1424, 1808, 1809
BsaAI YACGTR 4 cut(s) 211, 1391, 1579, 1770
BsaHI GRCGYC 2 cut(s) 444, 1074
BsaI GGTCTC 1 cut(s) 826
BsaJI CCNNGG 9 cut(s) 309, 369, 651, 708, 838, 874, 1203, 1806, 1807
BsaWI WCCGGW 1 cut(s) 1727
BsaXI ACNNNNNCTCC 6 cut(s) 200, 230, 1223, 1253, 1787, 1817
Bse118I RCCGGY 5 cut(s) 578, 1025, 1046, 1163, 1501
Bse1I ACTGG 3 cut(s) 318, 646, 1752
Bse3DI GCAATG 1 cut(s) 1473
BseBI CCWGG 4 cut(s) 311, 652, 797, 1685
BseDI CCNNGG 9 cut(s) 309, 369, 651, 708, 838, 874, 1203, 1806, 1807
BseGI GGATG 4 cut(s) 423, 666, 1415, 1857
BseMI GCAATG 1 cut(s) 1473
BseMII CTCAG 1 cut(s) 1421
BseNI ACTGG 3 cut(s) 318, 646, 1752
BseRI GAGGAG 5 cut(s) 377, 380, 383, 395, 749
BseSI GKGCMC 3 cut(s) 1253, 1413, 1769
BseXI GCAGC 5 cut(s) 900, 1463, 1517, 1545, 1596
BseYI CCCAGC 2 cut(s) 637, 1447
Bsh1236I CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
Bsh1285I CGRYCG 1 cut(s) 526
BshNI GGYRCC 1 cut(s) 1724
BsiEI CGRYCG 1 cut(s) 526
BsiHKAI GWGCWC 1 cut(s) 1769
BsiHKCI CYCGRG 1 cut(s) 1807
BslFI GGGAC 4 cut(s) 227, 602, 1458, 1789
BsmAI GTCTC 2 cut(s) 826, 1274
BsmFI GGGAC 4 cut(s) 227, 602, 1458, 1789
BsmI GAATGC 1 cut(s) 843
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 1807
Bsp119I TTCGAA 1 cut(s) 286
Bsp120I GGGCCC 1 cut(s) 1409
Bsp1286I GDGCHC 5 cut(s) 984, 1253, 1413, 1638, 1769
Bsp143I GATC 4 cut(s) 260, 534, 1129, 1747
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 1422
BspFNI CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
BspHI TCATGA 1 cut(s) 1668
BspLI GGNNCC 9 cut(s) 242, 243, 375, 1220, 1410, 1411, 1473, 1474, 1726
BspMAI CTGCAG 1 cut(s) 1563
BspPI GGATC 2 cut(s) 1137, 1742
BspQI GCTCTTC 1 cut(s) 1511
BspT104I TTCGAA 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 1724
BspTNI GGTCTC 1 cut(s) 826
BsrBI CCGCTC 1 cut(s) 625
BsrDI GCAATG 1 cut(s) 1473
BsrFI RCCGGY 5 cut(s) 578, 1025, 1046, 1163, 1501
BsrI ACTGG 3 cut(s) 318, 646, 1752
BssAI RCCGGY 5 cut(s) 578, 1025, 1046, 1163, 1501
BssECI CCNNGG 9 cut(s) 309, 369, 651, 708, 838, 874, 1203, 1806, 1807
BssMI GATC 4 cut(s) 260, 534, 1129, 1747
BssNI GRCGYC 2 cut(s) 444, 1074
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 4 cut(s) 311, 652, 797, 1685
Bst4CI ACNGT 4 cut(s) 460, 595, 601, 882
Bst6I CTCTTC 2 cut(s) 344, 1511
BstACI GRCGYC 2 cut(s) 444, 1074
BstBAI YACGTR 4 cut(s) 211, 1391, 1579, 1770
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 9 cut(s) 303, 627, 1027, 1253, 1401, 1456, 1529, 1604, 1656
BstDEI CTNAG 2 cut(s) 630, 1430
BstDSI CCRYGG 2 cut(s) 369, 708
BstEII GGTNACC 2 cut(s) 313, 1166
BstF5I GGATG 4 cut(s) 423, 666, 1415, 1857
BstFNI CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
BstH2I RGCGCY 2 cut(s) 779, 1559
BstHHI GCGC 7 cut(s) 687, 754, 778, 1297, 1480, 1495, 1558
BstKTI GATC 4 cut(s) 263, 537, 1132, 1750
BstMAI GTCTC 2 cut(s) 826, 1274
BstMBI GATC 4 cut(s) 260, 534, 1129, 1747
BstMCI CGRYCG 1 cut(s) 526
BstMWI GCNNNNNNNGC 7 cut(s) 631, 773, 870, 1236, 1261, 1612, 1621
BstNI CCWGG 4 cut(s) 311, 652, 797, 1685
BstNSI RCATGY 1 cut(s) 1069
BstPI GGTNACC 2 cut(s) 313, 1166
BstSFI CTRYAG 1 cut(s) 1559
BstSLI GKGCMC 3 cut(s) 1253, 1413, 1769
BstSNI TACGTA 2 cut(s) 1391, 1579
BstUI CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
BstV1I GCAGC 5 cut(s) 900, 1463, 1517, 1545, 1596
BstV2I GAAGAC 1 cut(s) 1128
BstX2I RGATCY 2 cut(s) 534, 1129
BstXI CCANNNNNNTGG 1 cut(s) 1691
BstYI RGATCY 2 cut(s) 534, 1129
BtgI CCRYGG 2 cut(s) 369, 708
BtgZI GCGATG 3 cut(s) 568, 733, 917
BtrI CACGTC 7 cut(s) 169, 421, 732, 1126, 1234, 1378, 1764
BtsCI GGATG 4 cut(s) 423, 666, 1415, 1857
BtsI GCAGTG 1 cut(s) 1594
BtsIMutI CAGTG 3 cut(s) 1427, 1594, 1650
Cac8I GCNNGC 9 cut(s) 303, 627, 1027, 1253, 1401, 1456, 1529, 1604, 1656
CciI TCATGA 1 cut(s) 1668
CfoI GCGC 7 cut(s) 687, 754, 778, 1297, 1480, 1495, 1558
Cfr10I RCCGGY 5 cut(s) 578, 1025, 1046, 1163, 1501
Cfr9I CCCGGG 1 cut(s) 1807
CseI GACGC 5 cut(s) 433, 517, 1082, 1094, 1559
Csp6I GTAC 4 cut(s) 324, 1117, 1210, 1771
CviAII CATG 5 cut(s) 370, 1066, 1102, 1669, 1691
CviQI GTAC 4 cut(s) 324, 1117, 1210, 1771
DdeI CTNAG 2 cut(s) 630, 1430
DpnI GATC 4 cut(s) 262, 536, 1131, 1749
DpnII GATC 4 cut(s) 260, 534, 1129, 1747
DraI TTTAAA 1 cut(s) 999
DrdI GACNNNNNNGTC 1 cut(s) 554
DseDI GACNNNNNNGTC 1 cut(s) 554
EaeI YGGCCR 2 cut(s) 798, 1098
Eam1104I CTCTTC 2 cut(s) 344, 1511
EarI CTCTTC 2 cut(s) 344, 1511
EciI GGCGGA 3 cut(s) 400, 424, 1802
Eco105I TACGTA 2 cut(s) 1391, 1579
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 3 cut(s) 984, 1413, 1638
Eco31I GGTCTC 1 cut(s) 826
Eco47I GGWCC 4 cut(s) 241, 373, 742, 1472
Eco57I CTGAAG 1 cut(s) 1334
Eco88I CYCGRG 1 cut(s) 1807
Eco91I GGTNACC 2 cut(s) 313, 1166
EcoO109I RGGNCCY 4 cut(s) 241, 742, 1409, 1472
EcoO65I GGTNACC 2 cut(s) 313, 1166
EcoRII CCWGG 4 cut(s) 309, 650, 795, 1683
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 3 cut(s) 984, 1413, 1638
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 373, 1069, 1105, 1672, 1694
FalI AAGNNNNNCTT 2 cut(s) 67, 99
FaqI GGGAC 4 cut(s) 227, 602, 1458, 1789
FatI CATG 5 cut(s) 369, 1065, 1101, 1668, 1690
FauI CCCGC 7 cut(s) 231, 559, 630, 690, 1483, 1504, 1828
FauNDI CATATG 1 cut(s) 297
FokI GGATG 4 cut(s) 410, 653, 1402, 1864
FriOI GRGCYC 3 cut(s) 984, 1413, 1638
FspBI CTAG 2 cut(s) 234, 1133
GlaI GCGC 7 cut(s) 686, 753, 777, 1296, 1479, 1494, 1557
GsaI CCCAGC 2 cut(s) 641, 1451
HaeII RGCGCY 2 cut(s) 779, 1559
HgaI GACGC 5 cut(s) 433, 517, 1082, 1094, 1559
HhaI GCGC 7 cut(s) 687, 754, 778, 1297, 1480, 1495, 1558
Hin1I GRCGYC 2 cut(s) 444, 1074
Hin1II CATG 5 cut(s) 373, 1069, 1105, 1672, 1694
Hin6I GCGC 7 cut(s) 685, 752, 776, 1295, 1478, 1493, 1556
HinP1I GCGC 7 cut(s) 685, 752, 776, 1295, 1478, 1493, 1556
HincII GTYRAC 4 cut(s) 187, 448, 961, 1054
HindII GTYRAC 4 cut(s) 187, 448, 961, 1054
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 4 cut(s) 123, 702, 1432, 1731
HpaI GTTAAC 1 cut(s) 1054
HphI GGTGA 8 cut(s) 421, 583, 589, 799, 978, 1178, 1491, 1729
Hpy166II GTNNAC 8 cut(s) 187, 214, 448, 961, 1054, 1372, 1597, 1767
Hpy188I TCNGA 4 cut(s) 736, 878, 1330, 1549
Hpy188III TCNNGA 6 cut(s) 258, 505, 532, 823, 1279, 1669
Hpy8I GTNNAC 8 cut(s) 187, 214, 448, 961, 1054, 1372, 1597, 1767
Hpy99I CGWCG 6 cut(s) 123, 605, 736, 1076, 1379, 1553
HpyCH4III ACNGT 4 cut(s) 460, 595, 601, 882
HpyCH4V TGCA 4 cut(s) 1561, 1584, 1602, 1767
HpyF10VI GCNNNNNNNGC 7 cut(s) 631, 773, 870, 1236, 1261, 1612, 1621
HpyF3I CTNAG 2 cut(s) 630, 1430
Hsp92I GRCGYC 2 cut(s) 444, 1074
Hsp92II CATG 5 cut(s) 373, 1069, 1105, 1672, 1694
HspAI GCGC 7 cut(s) 685, 752, 776, 1295, 1478, 1493, 1556
KflI GGGWCCC 2 cut(s) 241, 1472
KroI GCCGGC 1 cut(s) 1025
KroNI GCCGGC 1 cut(s) 1027
KspAI GTTAAC 1 cut(s) 1054
Kzo9I GATC 4 cut(s) 260, 534, 1129, 1747
LguI GCTCTTC 1 cut(s) 1511
LmnI GCTCC 6 cut(s) 390, 639, 783, 1244, 1330, 1687
Lsp1109I GCAGC 5 cut(s) 900, 1463, 1517, 1545, 1596
LweI GCATC 4 cut(s) 591, 763, 1251, 1448
MaeI CTAG 2 cut(s) 234, 1133
MaeIII GTNAC 9 cut(s) 77, 313, 589, 595, 805, 1166, 1391, 1735, 1754
MalI GATC 4 cut(s) 262, 536, 1131, 1749
MbiI CCGCTC 1 cut(s) 625
MboI GATC 4 cut(s) 260, 534, 1129, 1747
MboII GAAGA 7 cut(s) 331, 529, 1117, 1133, 1282, 1528, 1576
MflI RGATCY 2 cut(s) 534, 1129
MhlI GDGCHC 5 cut(s) 984, 1253, 1413, 1638, 1769
MlsI TGGCCA 1 cut(s) 800
MluCI AATT 2 cut(s) 1336, 1639
MluNI TGGCCA 1 cut(s) 800
MlyI GAGTC 2 cut(s) 1441, 1740
MmeI TCCRAC 3 cut(s) 714, 1308, 1572
Mox20I TGGCCA 1 cut(s) 800
MreI CGCCGGCG 1 cut(s) 1025
MroNI GCCGGC 1 cut(s) 1025
MscI TGGCCA 1 cut(s) 800
MseI TTAA 4 cut(s) 39, 998, 1053, 1908
MslI CAYNNNNRTG 1 cut(s) 1064
Msp20I TGGCCA 1 cut(s) 800
MspA1I CMGCKG 2 cut(s) 891, 1508
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 4 cut(s) 311, 652, 797, 1685
MvnI CGCG 8 cut(s) 528, 685, 752, 1088, 1295, 1478, 1553, 1658
MwoI GCNNNNNNNGC 7 cut(s) 631, 773, 870, 1236, 1261, 1612, 1621
NaeI GCCGGC 1 cut(s) 1027
NciI CCSGG 6 cut(s) 764, 1243, 1367, 1424, 1808, 1809
NcoI CCATGG 1 cut(s) 369
NdeI CATATG 1 cut(s) 297
NdeII GATC 4 cut(s) 260, 534, 1129, 1747
NgoMIV GCCGGC 1 cut(s) 1025
NlaIII CATG 5 cut(s) 373, 1069, 1105, 1672, 1694
NlaIV GGNNCC 9 cut(s) 242, 243, 375, 1220, 1410, 1411, 1473, 1474, 1726
NmeAIII GCCGAG 4 cut(s) 764, 819, 1076, 1228
NmuCI GTSAC 5 cut(s) 589, 595, 805, 1166, 1735
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 1 cut(s) 286
PagI TCATGA 1 cut(s) 1668
PciI ACATGT 1 cut(s) 1065
PciSI GCTCTTC 1 cut(s) 1511
PcsI WCGNNNNNNNCGW 3 cut(s) 591, 1188, 1662
PctI GAATGC 1 cut(s) 843
PdiI GCCGGC 1 cut(s) 1027
PfeI GAWTC 2 cut(s) 123, 702
PflFI GACNNNGTC 2 cut(s) 527, 902
PfoI TCCNGGA 1 cut(s) 1241
PleI GAGTC 2 cut(s) 1440, 1739
PpsI GAGTC 2 cut(s) 1440, 1739
Ppu21I YACGTR 4 cut(s) 211, 1391, 1579, 1770
PpuMI RGGWCCY 3 cut(s) 241, 742, 1472
PscI ACATGT 1 cut(s) 1065
Psp1406I AACGTT 1 cut(s) 1542
Psp5II RGGWCCY 3 cut(s) 241, 742, 1472
Psp6I CCWGG 4 cut(s) 309, 650, 795, 1683
PspEI GGTNACC 2 cut(s) 313, 1166
PspFI CCCAGC 2 cut(s) 637, 1447
PspGI CCWGG 4 cut(s) 309, 650, 795, 1683
PspN4I GGNNCC 9 cut(s) 242, 243, 375, 1220, 1410, 1411, 1473, 1474, 1726
PspOMI GGGCCC 1 cut(s) 1409
PspPPI RGGWCCY 3 cut(s) 241, 742, 1472
PsrI GAACNNNNNNTAC 4 cut(s) 1559, 1591, 1664, 1696
PstI CTGCAG 1 cut(s) 1563
PsuI RGATCY 2 cut(s) 534, 1129
PsyI GACNNNGTC 2 cut(s) 527, 902
RsaI GTAC 4 cut(s) 325, 1118, 1211, 1772
RsaNI GTAC 4 cut(s) 324, 1117, 1210, 1771
RseI CAYNNNNRTG 1 cut(s) 1064
SapI GCTCTTC 1 cut(s) 1511
SaqAI TTAA 4 cut(s) 39, 998, 1053, 1908
Sau3AI GATC 4 cut(s) 260, 534, 1129, 1747
SchI GAGTC 2 cut(s) 1441, 1740
SduI GDGCHC 5 cut(s) 984, 1253, 1413, 1638, 1769
SfaNI GCATC 4 cut(s) 591, 763, 1251, 1448
SfcI CTRYAG 1 cut(s) 1559
SfuI TTCGAA 1 cut(s) 286
SgrAI CRCCGGYG 2 cut(s) 1025, 1046
SinI GGWCC 4 cut(s) 241, 373, 742, 1472
SmaI CCCGGG 1 cut(s) 1809
SmiMI CAYNNNNRTG 1 cut(s) 1064
SmlI CTYRAG 1 cut(s) 503
SmoI CTYRAG 1 cut(s) 503
SnaBI TACGTA 2 cut(s) 1391, 1579
Sse9I AATT 2 cut(s) 1336, 1639
SspI AATATT 1 cut(s) 1912
SspMI CTAG 2 cut(s) 234, 1133
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 4 cut(s) 460, 595, 601, 882
TaqI TCGA 8 cut(s) 286, 522, 824, 1035, 1071, 1278, 1362, 1538
TasI AATT 2 cut(s) 1336, 1639
TauI GCSGC 5 cut(s) 644, 894, 1511, 1556, 1661
TfiI GAWTC 2 cut(s) 123, 702
Tru1I TTAA 4 cut(s) 39, 998, 1053, 1908
Tru9I TTAA 4 cut(s) 39, 998, 1053, 1908
TscAI CASTG 3 cut(s) 1434, 1594, 1650
TseFI GTSAC 5 cut(s) 589, 595, 805, 1166, 1735
TseI GCWGC 5 cut(s) 888, 1451, 1505, 1558, 1584
Tsp45I GTSAC 5 cut(s) 589, 595, 805, 1166, 1735
TspDTI ATGAA 5 cut(s) 87, 270, 785, 1118, 1685
TspGWI ACGGA 3 cut(s) 13, 172, 551
TspMI CCCGGG 1 cut(s) 1807
TspRI CASTG 3 cut(s) 1434, 1594, 1650
Tth111I GACNNNGTC 2 cut(s) 527, 902
VneI GTGCAC 1 cut(s) 1765
VpaK11BI GGWCC 4 cut(s) 241, 373, 742, 1472
XceI RCATGY 1 cut(s) 1069
XmaI CCCGGG 1 cut(s) 1807
XspI CTAG 2 cut(s) 234, 1133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.