RchiOBHm_Chr5g0064901

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016534
Physical Location & Seq
Reverse (-)
70831914 .. 70833595
1682 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34082

Sequence Viewer

Length: 1284 bp
ATGGAACCCAAGAAGCTCCTCCTCCTCTTCCTCTTCTTTCTTTTCCACTCCGCCTGCCACGTCATCCTCACAGCCGCCATCGGCGTCAACTACGGCACAGTAGCCGACAACCTCCCGCCCCCGTCCCAGGTCGCCACCTTCCTCAAGACCAAGACCACCATCGACCGCGTCAAGATCTTCGACACCAACCCCGACGTCCTCCGCGCATTCGCCAACACCGGCATCGCCGTCACCGTCACCGTCGGTAACGGCGACTTCCCGCCCCTCGCCAAGCTCCCCGCCGCCCAGTCCTGGGTGGCCGACAACATCCTCCCCTTCTATCCCCGCACCAACATCAACCGAATCGCCGTCGGGAACGAAATCCTCGCCACGTCGGACAAGGACCTCATCGCGCATCTCCTGCCGGCTATGAAGGCGCTCCACTCGGCTCTCAACCTCGCCAACATCACCGCCATTCAGGTCTCGACACCTCACTCGCTCGGCATACTCGCCCCCAACACGGGCCCACCGAGCCAAGGACAGTTCCGGACCGGCTACGACAAGATCTTGGAGTACCATCGCCAGACCAAATCGCCGTTCCTCGTCAACCCGTACCCGTTTTTTGGTCTCTCACCCGATACGCTCAACTACGCGACTTTCAGGCCAAACGGCGGCGTTTTCGACAATGCCACCGGCATGAACTACACCAACATGTTCGACGCCCAGATGGACGCGGTTTTCTCCGCGATGAAGAAGGTCGGGCATGAAGACGTGGATCTAGTAGTCGCCGAAACCGGGTGGCCCTCCGCGGGTGACCCGAACCAACTCGGTGTCAGCTTGGAAAACGCGGTGTCGTACAACGGGAACCTCATCCGCCACGTTAGATCCGGGAAGGGCACGCCGTTGATGCCGAACAGGACGTTCGAGACTTACATTTTCGCGCTGTTCAATGAGAACCTGAAGCCCACGCTGTCGGAGCAGAATTACGGGCTGTTCAAACCCGACCTCGACCCGGTTTACGACGTCGTGCTCTGCGTCTCTGACACACTGCGTCCCAGCTTTTTCAAAAATGAGTCGGGCCCCACATCCGGCCCGGCATCAGGCTCATCAGCGAGTACAGCAACAGGCCCAGCAGCGATGGGTCCCACGCGCGATGTAGTTGCGCAACCACCGTCGTCGACGGGTAAGAGGTGGTGCGTGCCGAAACCGAACGCTACCGAGGCGACGCTGCAGAAGAACATAGACTACGTTTGCAGCAGCGGAGTGGACTGCAAGCCCATACAGAATGGTGGGCTACATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

427

Amino Acids

46.03

Weight (kDa)

6.75

Isoelectric Point (pI)

35.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 27 - 335 8.8e-77 Glycosyl hydrolases family 17
X8 PF07983 391 - 424 3e-07 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46570
fragaria_vesca FvH4_3g36150
malus_domestica MD11G1107600.v1.1
prunus_persica Prupe.6G076900_v2.0.a1
pyrus_communis pycom11g08910
rosa_chinensis RchiOBHm_Chr5g0064591 RchiOBHm_Chr5g0064901
rosa_laevigata RLG00000035739
rosa_roxburghii Rroxscaffold_1G00016410
rosa_rugosa Rorug05G0366600
rosa_samantha Rh5AG424700 Rh5BG439700 Rh5DG453000
rosa_wichuraiana Rw5G039930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 198, 1005
Acc16I TGCGCA 1 cut(s) 1143
AccI GTMKAC 1 cut(s) 1157
AccIII TCCGGA 1 cut(s) 525
AclWI GGATC 2 cut(s) 762, 858
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 959
AcyI GRCGYC 4 cut(s) 84, 195, 699, 1002
AfaI GTAC 4 cut(s) 554, 593, 836, 1096
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 3 cut(s) 928, 976, 1045
AjiI CACGTC 3 cut(s) 61, 372, 751
AjnI CCWGG 2 cut(s) 126, 290
AjuI GAANNNNNNNTTGG 2 cut(s) 560, 592
AluBI AGCT 4 cut(s) 16, 274, 816, 1038
AluI AGCT 4 cut(s) 16, 274, 816, 1038
Alw21I GWGCWC 1 cut(s) 1011
Alw26I GTCTC 4 cut(s) 466, 611, 899, 1021
AlwI GGATC 2 cut(s) 762, 858
Aor13HI TCCGGA 1 cut(s) 525
AoxI GGCC 7 cut(s) 297, 502, 641, 779, 1057, 1069, 1105
ApaI GGGCCC 2 cut(s) 506, 1061
ApeKI GCWGC 4 cut(s) 1112, 1207, 1233, 1236
AspLEI GCGC 6 cut(s) 206, 394, 418, 922, 1131, 1144
AsuC2I CCSGG 4 cut(s) 775, 868, 992, 1073
AsuHPI GGTGA 5 cut(s) 223, 229, 439, 603, 803
AvaII GGWCC 3 cut(s) 382, 528, 1121
BaeGI GKGCMC 3 cut(s) 506, 878, 1061
BanII GRGCYC 2 cut(s) 506, 1061
BbsI GAAGAC 1 cut(s) 753
Bbv12I GWGCWC 1 cut(s) 1011
BbvI GCAGC 4 cut(s) 1124, 1194, 1245, 1248
BccI CCATC 5 cut(s) 86, 167, 564, 700, 1111
BceAI ACGGC 7 cut(s) 109, 212, 265, 332, 559, 664, 865
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciT130I CCWGG 2 cut(s) 128, 292
BcnI CCSGG 4 cut(s) 775, 868, 992, 1073
BcoDI GTCTC 4 cut(s) 466, 611, 899, 1021
BfaI CTAG 1 cut(s) 758
BfmI CTRYAG 1 cut(s) 1208
BfoI RGCGCY 1 cut(s) 419
BglII AGATCT 2 cut(s) 174, 543
BisI GCNGC 7 cut(s) 75, 282, 652, 1113, 1208, 1234, 1237
BlsI GCNGC 7 cut(s) 76, 283, 653, 1114, 1209, 1235, 1238
Bme1390I CCNGG 6 cut(s) 128, 292, 775, 868, 992, 1073
Bme18I GGWCC 3 cut(s) 382, 528, 1121
BmgBI CACGTC 3 cut(s) 61, 372, 751
BmiI GGNNCC 7 cut(s) 6, 504, 845, 1059, 1060, 1122, 1123
BmrFI CCNGG 6 cut(s) 128, 292, 775, 868, 992, 1073
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 4 cut(s) 231, 403, 876, 1085
BmuI ACTGGG 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 4 cut(s) 775, 868, 992, 1073
BsaHI GRCGYC 4 cut(s) 84, 195, 699, 1002
BsaI GGTCTC 2 cut(s) 466, 611
BsaJI CCNNGG 5 cut(s) 126, 291, 514, 786, 1197
BsaWI WCCGGW 1 cut(s) 525
Bse118I RCCGGY 4 cut(s) 218, 403, 530, 671
Bse1I ACTGG 1 cut(s) 286
BseAI TCCGGA 1 cut(s) 525
BseBI CCWGG 2 cut(s) 128, 292
BseDI CCNNGG 5 cut(s) 126, 291, 514, 786, 1197
BseGI GGATG 4 cut(s) 63, 306, 849, 1064
BseNI ACTGG 1 cut(s) 286
BseRI GAGGAG 3 cut(s) 8, 11, 14
BseSI GKGCMC 3 cut(s) 506, 878, 1061
BseXI GCAGC 4 cut(s) 1124, 1194, 1245, 1248
BseYI CCCAGC 2 cut(s) 1034, 1108
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 7 cut(s) 299, 504, 643, 781, 1059, 1071, 1107
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 1011
BslFI GGGAC 3 cut(s) 109, 1017, 1107
BsmAI GTCTC 4 cut(s) 466, 611, 899, 1021
BsmBI CGTCTC 1 cut(s) 1021
BsmFI GGGAC 3 cut(s) 109, 1017, 1107
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 7 cut(s) 299, 504, 643, 781, 1059, 1071, 1107
Bso31I GGTCTC 2 cut(s) 466, 611
Bsp120I GGGCCC 2 cut(s) 502, 1057
Bsp1286I GDGCHC 4 cut(s) 506, 878, 1011, 1061
Bsp13I TCCGGA 1 cut(s) 525
Bsp143I GATC 4 cut(s) 174, 543, 754, 863
BspANI GGCC 7 cut(s) 299, 504, 643, 781, 1059, 1071, 1107
BspEI TCCGGA 1 cut(s) 525
BspLI GGNNCC 7 cut(s) 6, 504, 845, 1059, 1060, 1122, 1123
BspMAI CTGCAG 1 cut(s) 1212
BspPI GGATC 2 cut(s) 762, 858
BspTNI GGTCTC 2 cut(s) 466, 611
BsrFI RCCGGY 4 cut(s) 218, 403, 530, 671
BsrI ACTGG 1 cut(s) 286
BssAI RCCGGY 4 cut(s) 218, 403, 530, 671
BssECI CCNNGG 5 cut(s) 126, 291, 514, 786, 1197
BssMI GATC 4 cut(s) 174, 543, 754, 863
BssNI GRCGYC 4 cut(s) 84, 195, 699, 1002
BssT1I CCWWGG 1 cut(s) 514
Bst2UI CCWGG 2 cut(s) 128, 292
Bst4CI ACNGT 5 cut(s) 100, 235, 241, 522, 1152
Bst6I CTCTTC 2 cut(s) 32, 38
BstACI GRCGYC 4 cut(s) 84, 195, 699, 1002
BstAPI GCANNNNNTGC 1 cut(s) 400
BstC8I GCNNGC 5 cut(s) 55, 405, 878, 1178, 1253
BstDSI CCRYGG 1 cut(s) 786
BstEII GGTNACC 1 cut(s) 791
BstF5I GGATG 4 cut(s) 63, 306, 849, 1064
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 6 cut(s) 206, 394, 418, 922, 1131, 1144
BstKTI GATC 4 cut(s) 177, 546, 757, 866
BstMAI GTCTC 4 cut(s) 466, 611, 899, 1021
BstMBI GATC 4 cut(s) 174, 543, 754, 863
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 7 cut(s) 400, 413, 510, 886, 955, 1097, 1199
BstNI CCWGG 2 cut(s) 128, 292
BstNSI RCATGY 1 cut(s) 694
BstPI GGTNACC 1 cut(s) 791
BstSCI CCNGG 6 cut(s) 126, 290, 773, 866, 990, 1071
BstSFI CTRYAG 1 cut(s) 1208
BstSLI GKGCMC 3 cut(s) 506, 878, 1061
BstV1I GCAGC 4 cut(s) 1124, 1194, 1245, 1248
BstV2I GAAGAC 1 cut(s) 753
BstX2I RGATCY 4 cut(s) 174, 543, 754, 863
BstYI RGATCY 4 cut(s) 174, 543, 754, 863
BsuRI GGCC 7 cut(s) 299, 504, 643, 781, 1059, 1071, 1107
BtgI CCRYGG 1 cut(s) 786
BtgZI GCGATG 6 cut(s) 208, 373, 542, 740, 1130, 1146
BtrI CACGTC 3 cut(s) 61, 372, 751
BtsCI GGATG 4 cut(s) 63, 306, 849, 1064
BtsI GCAGTG 1 cut(s) 1025
BtsIMutI CAGTG 1 cut(s) 1025
Cac8I GCNNGC 5 cut(s) 55, 405, 878, 1178, 1253
CfoI GCGC 6 cut(s) 206, 394, 418, 922, 1131, 1144
Cfr10I RCCGGY 4 cut(s) 218, 403, 530, 671
Cfr42I CCGCGG 1 cut(s) 789
CpoI CGGWCCG 1 cut(s) 528
CseI GACGC 7 cut(s) 73, 157, 707, 719, 1003, 1019, 1213
Csp6I GTAC 4 cut(s) 553, 592, 835, 1095
CspI CGGWCCG 1 cut(s) 528
CviAII CATG 3 cut(s) 676, 691, 743
CviQI GTAC 4 cut(s) 553, 592, 835, 1095
DpnI GATC 4 cut(s) 176, 545, 756, 865
DpnII GATC 4 cut(s) 174, 543, 754, 863
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 32, 38
EarI CTCTTC 2 cut(s) 32, 38
EciI GGCGGA 2 cut(s) 40, 842
Eco130I CCWWGG 1 cut(s) 514
Eco24I GRGCYC 2 cut(s) 506, 1061
Eco31I GGTCTC 2 cut(s) 466, 611
Eco47I GGWCC 3 cut(s) 382, 528, 1121
Eco57I CTGAAG 1 cut(s) 959
Eco91I GGTNACC 1 cut(s) 791
EcoO109I RGGNCCY 3 cut(s) 382, 1058, 1121
EcoO65I GGTNACC 1 cut(s) 791
EcoRII CCWGG 2 cut(s) 126, 290
EcoT14I CCWWGG 1 cut(s) 514
EcoT38I GRGCYC 2 cut(s) 506, 1061
ErhI CCWWGG 1 cut(s) 514
Esp3I CGTCTC 1 cut(s) 1021
FaeI CATG 3 cut(s) 679, 694, 746
FaiI YATR 7 cut(s) 410, 485, 677, 692, 744, 1220, 1259
FaqI GGGAC 3 cut(s) 109, 1017, 1107
FatI CATG 3 cut(s) 675, 690, 742
FauI CCCGC 5 cut(s) 123, 267, 286, 332, 781
FblI GTMKAC 1 cut(s) 1157
Fnu4HI GCNGC 7 cut(s) 75, 282, 652, 1113, 1208, 1234, 1237
FokI GGATG 4 cut(s) 50, 293, 836, 1051
FriOI GRGCYC 2 cut(s) 506, 1061
Fsp4HI GCNGC 7 cut(s) 75, 282, 652, 1113, 1208, 1234, 1237
FspBI CTAG 1 cut(s) 758
FspI TGCGCA 1 cut(s) 1143
GlaI GCGC 6 cut(s) 205, 393, 417, 921, 1130, 1143
GluI GCNGC 7 cut(s) 75, 282, 652, 1113, 1208, 1234, 1237
GsaI CCCAGC 2 cut(s) 1038, 1112
HaeII RGCGCY 1 cut(s) 419
HaeIII GGCC 7 cut(s) 299, 504, 643, 781, 1059, 1071, 1107
HgaI GACGC 7 cut(s) 73, 157, 707, 719, 1003, 1019, 1213
HhaI GCGC 6 cut(s) 206, 394, 418, 922, 1131, 1144
Hin1I GRCGYC 4 cut(s) 84, 195, 699, 1002
Hin1II CATG 3 cut(s) 679, 694, 746
Hin6I GCGC 6 cut(s) 204, 392, 416, 920, 1129, 1142
HinP1I GCGC 6 cut(s) 204, 392, 416, 920, 1129, 1142
HincII GTYRAC 3 cut(s) 88, 586, 1158
HindII GTYRAC 3 cut(s) 88, 586, 1158
HinfI GANTC 2 cut(s) 342, 1052
HphI GGTGA 5 cut(s) 223, 229, 439, 603, 803
Hpy166II GTNNAC 5 cut(s) 88, 586, 997, 1158, 1246
Hpy188I TCNGA 3 cut(s) 376, 955, 1021
Hpy188III TCNNGA 6 cut(s) 145, 172, 352, 463, 526, 904
Hpy8I GTNNAC 5 cut(s) 88, 586, 997, 1158, 1246
HpyAV CCTTC 5 cut(s) 148, 325, 406, 727, 865
HpyCH4III ACNGT 5 cut(s) 100, 235, 241, 522, 1152
HpyCH4IV ACGT 8 cut(s) 60, 195, 371, 750, 858, 899, 1002, 1227
HpyCH4V TGCA 3 cut(s) 1210, 1233, 1251
HpyF10VI GCNNNNNNNGC 7 cut(s) 400, 413, 510, 886, 955, 1097, 1199
HpySE526I ACGT 8 cut(s) 60, 195, 371, 750, 858, 899, 1002, 1227
Hsp92I GRCGYC 4 cut(s) 84, 195, 699, 1002
Hsp92II CATG 3 cut(s) 679, 694, 746
HspAI GCGC 6 cut(s) 204, 392, 416, 920, 1129, 1142
KflI GGGWCCC 1 cut(s) 1121
Kpn2I TCCGGA 1 cut(s) 525
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
KspI CCGCGG 1 cut(s) 789
Kzo9I GATC 4 cut(s) 174, 543, 754, 863
LmnI GCTCC 4 cut(s) 21, 279, 423, 955
Lsp1109I GCAGC 4 cut(s) 1124, 1194, 1245, 1248
LweI GCATC 4 cut(s) 231, 403, 876, 1085
MaeI CTAG 1 cut(s) 758
MaeII ACGT 8 cut(s) 60, 195, 371, 750, 858, 899, 1002, 1227
MaeIII GTNAC 4 cut(s) 229, 235, 245, 791
MalI GATC 4 cut(s) 176, 545, 756, 865
MboI GATC 4 cut(s) 174, 543, 754, 863
MboII GAAGA 6 cut(s) 19, 25, 169, 742, 758, 1225
MflI RGATCY 4 cut(s) 174, 543, 754, 863
MhlI GDGCHC 4 cut(s) 506, 878, 1011, 1061
MluCI AATT 1 cut(s) 961
MlyI GAGTC 1 cut(s) 1061
MmeI TCCRAC 2 cut(s) 354, 933
MroI TCCGGA 1 cut(s) 525
MroNI GCCGGC 1 cut(s) 403
MslI CAYNNNNRTG 2 cut(s) 674, 689
MspA1I CMGCKG 2 cut(s) 788, 1239
MspR9I CCNGG 6 cut(s) 128, 292, 775, 868, 992, 1073
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 2 cut(s) 128, 292
MwoI GCNNNNNNNGC 7 cut(s) 400, 413, 510, 886, 955, 1097, 1199
NaeI GCCGGC 1 cut(s) 405
NciI CCSGG 4 cut(s) 775, 868, 992, 1073
NdeII GATC 4 cut(s) 174, 543, 754, 863
NgoMIV GCCGGC 1 cut(s) 403
NlaIII CATG 3 cut(s) 679, 694, 746
NlaIV GGNNCC 7 cut(s) 6, 504, 845, 1059, 1060, 1122, 1123
NmeAIII GCCGAG 2 cut(s) 404, 459
NmuCI GTSAC 3 cut(s) 229, 235, 791
NsbI TGCGCA 1 cut(s) 1143
NspI RCATGY 1 cut(s) 694
PciI ACATGT 1 cut(s) 690
PcsI WCGNNNNNNNCGW 4 cut(s) 231, 249, 506, 1011
PctI GAATGC 1 cut(s) 206
PdiI GCCGGC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 342
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 866
PkrI GCNGC 7 cut(s) 76, 283, 653, 1114, 1209, 1235, 1238
PleI GAGTC 1 cut(s) 1060
PpsI GAGTC 1 cut(s) 1060
PpuMI RGGWCCY 2 cut(s) 382, 1121
PscI ACATGT 1 cut(s) 690
Psp5II RGGWCCY 2 cut(s) 382, 1121
Psp6I CCWGG 2 cut(s) 126, 290
PspEI GGTNACC 1 cut(s) 791
PspFI CCCAGC 2 cut(s) 1034, 1108
PspGI CCWGG 2 cut(s) 126, 290
PspN4I GGNNCC 7 cut(s) 6, 504, 845, 1059, 1060, 1122, 1123
PspOMI GGGCCC 2 cut(s) 502, 1057
PspPPI RGGWCCY 2 cut(s) 382, 1121
PsrI GAACNNNNNNTAC 2 cut(s) 1208, 1240
PstI CTGCAG 1 cut(s) 1212
PsuI RGATCY 4 cut(s) 174, 543, 754, 863
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 4 cut(s) 554, 593, 836, 1096
RsaNI GTAC 4 cut(s) 553, 592, 835, 1095
RseI CAYNNNNRTG 2 cut(s) 674, 689
Rsr2I CGGWCCG 1 cut(s) 528
RsrII CGGWCCG 1 cut(s) 528
SacII CCGCGG 1 cut(s) 789
SalI GTCGAC 1 cut(s) 1156
SatI GCNGC 7 cut(s) 75, 282, 652, 1113, 1208, 1234, 1237
Sau3AI GATC 4 cut(s) 174, 543, 754, 863
SchI GAGTC 1 cut(s) 1061
ScrFI CCNGG 6 cut(s) 128, 292, 775, 868, 992, 1073
SduI GDGCHC 4 cut(s) 506, 878, 1011, 1061
SfaNI GCATC 4 cut(s) 231, 403, 876, 1085
SfcI CTRYAG 1 cut(s) 1208
Sfr303I CCGCGG 1 cut(s) 789
SgrBI CCGCGG 1 cut(s) 789
SgrDI CGTCGACG 1 cut(s) 1156
SinI GGWCC 3 cut(s) 382, 528, 1121
SmiMI CAYNNNNRTG 2 cut(s) 674, 689
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 1 cut(s) 961
SspMI CTAG 1 cut(s) 758
StyD4I CCNGG 6 cut(s) 126, 290, 773, 866, 990, 1071
StyI CCWWGG 1 cut(s) 514
TaaI ACNGT 5 cut(s) 100, 235, 241, 522, 1152
TaiI ACGT 8 cut(s) 63, 198, 374, 753, 861, 902, 1005, 1230
TaqI TCGA 8 cut(s) 162, 180, 464, 660, 696, 903, 987, 1157
TasI AATT 1 cut(s) 961
TatI WGTACW 1 cut(s) 1094
TauI GCSGC 3 cut(s) 77, 284, 654
TfiI GAWTC 1 cut(s) 342
TscAI CASTG 1 cut(s) 1032
TseFI GTSAC 3 cut(s) 229, 235, 791
TseI GCWGC 4 cut(s) 1112, 1207, 1233, 1236
Tsp45I GTSAC 3 cut(s) 229, 235, 791
TspDTI ATGAA 4 cut(s) 425, 692, 743, 759
TspRI CASTG 1 cut(s) 1032
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 3 cut(s) 382, 528, 1121
XceI RCATGY 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 1157
XspI CTAG 1 cut(s) 758
ZraI GACGTC 2 cut(s) 196, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.