Rroxscaffold_1G00016410

Glucan endo-1,3-beta-glucosidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016534
Physical Location & Seq
Forward (+)
20420568 .. 20422725
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00016410.1

Sequence Viewer

Length: 1530 bp
ATGGAGCCCAAGAAGCTCCTCCTCCTCTTCCTCTTCTTCTTCTTCCACTCCGCCCGCCACGTCATCCTCACCGCCGCCATCGGCGTCAACTACGGTACAGTAGCCGACAACCTCCCGCCTCCGTCCCAGGTCGCCACCTTCCTCAAGACCAAGACCACCATCGACCGCGTCAAGATCTTCGACACCAACCCCGACGTCCTCCGCGCCTTCGCCAACACCGGCATCGCCGTCACCGTCACCGTCGGCAACGGCGACTTCCCGCCCCTCGCCAAGCTCCCCGCCGCCCAGTCCTGGGTGGCCGACAACATCCTCCCCTTCTATCCCCGCACCAACATCAACCGAATCGCCGTCGGGAACGAAATCCTCGCCACGTCGGACAAGGACCTGATCGCGCATCTCCTGCCGGCTATGAAGGCGCTCCACTCGGCTCTCAACCTCGCCAACGTCACCGCCATTCAGGTCTCGACACCTCACTCGCTCGGCATACTCGCCCCCAACACGGGCCCACCGAGCCTCGGACAGTTCCGGACCGGCTACGACAAGATCATATTCGAGCCGATCTTGGAGTACCATCGTCAGACCAAATCGCCGTTCCTCGTCAACCCGTACCCGTTTTTCGGACTCTCACCCGATACGCTCAACTACGCGACTTTCAGGCCAAACGGCGGCGTTTTCGACAATGCCACCGGCATGAACTACACCAACATGTTCGACGCCCAGATGGACGCGGTTTTCTCGGCGATGAAGAAGGTCGGGTATGAAGACGTGGATCTAGTAGTCGCCGAAACCGGTTGGCCCTCCGCGGGTGACCCGAACCAACTCGGTGTGAGCTTGGAAAACGCGGTTTCGTACAACGGGAACCTCATCCGGCACGTCAGCTCCGGGAAGGGCACGCCGTTGATGCCGAACAGGACGTTCGAGACTTACATTTTCGCGCTCTTCAATGAGAACCTGAAGCCCACGCTGTCGGAGCAGAATTACGGGCTGTTCAAACCCGACCTCGACCCGGTTTACGACGTCGGCCTTTTACGCAACGGACAGGCACAGGGCCCCACATCAGGCCCGGCATCAGGCCCATCAGCGAGTACCGCAGCAGGCCCAGCAGCAATGGGTCGCACGCCCGATGTTGTTGCGCAACCACCGTCGTCGACGGGGAAGAGGTGGTGCGTGCCGAAACCGAACGCTACGAAGGCGGCGCTGCAGAAGAACATAGACTACGTTTGCAGCAGCGGAGTGGATTGCAAGCCCATACAGAATGGTGGGCCGTGCTTTGAGCCCAACACTATGAAGTCGCATGCGGCTTACGTCATGAACGCTTATTACCAGGACCATGGCCCACAGGATATTAACTGCGATTTCAACGGCACCGGAGTGGTCACCACCGCTGATCCCAGTTACAAAACGTGCACGTACCCTAACAAGGCGGAGAAGGAGGCAAAGTCGGTGGCGGGAAGCTCAATGAGAATGAGATGTAGCAAAGTGGCTCTGTTCTTTCTAATGGTCGGTTGTGGTGTAATGTTTAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_17 X8

Protein Analysis

509

Amino Acids

54.91

Weight (kDa)

8.01

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 27 - 340 1.5e-77 Glycosyl hydrolases family 17
X8 PF07983 388 - 457 2.4e-23 X8 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G46570
fragaria_vesca FvH4_3g36150
malus_domestica MD11G1107600.v1.1
prunus_persica Prupe.6G076900_v2.0.a1
pyrus_communis pycom11g08910
rosa_chinensis RchiOBHm_Chr5g0064591 RchiOBHm_Chr5g0064901
rosa_laevigata RLG00000035739
rosa_roxburghii Rroxscaffold_1G00016410
rosa_rugosa Rorug05G0366600
rosa_samantha Rh5AG424700 Rh5BG439700 Rh5DG453000
rosa_wichuraiana Rw5G039930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 198, 1020
Acc16I TGCGCA 1 cut(s) 1134
AccB1I GGYRCC 1 cut(s) 1364
AccI GTMKAC 1 cut(s) 1148
AccII CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
AccIII TCCGGA 1 cut(s) 525
AclWI GGATC 2 cut(s) 777, 1382
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 1 cut(s) 974
AcyI GRCGYC 4 cut(s) 84, 195, 714, 1017
AfaI GTAC 6 cut(s) 97, 569, 608, 851, 1087, 1412
AflIII ACRYGT 1 cut(s) 705
AgeI ACCGGT 1 cut(s) 788
AgsI TTSAA 3 cut(s) 943, 991, 1360
AjiI CACGTC 4 cut(s) 61, 372, 766, 874
AjnI CCWGG 3 cut(s) 126, 290, 1323
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AleI CACNNNNGTG 1 cut(s) 1370
AluBI AGCT 5 cut(s) 16, 274, 831, 879, 1455
AluI AGCT 5 cut(s) 16, 274, 831, 879, 1455
Alw21I GWGCWC 1 cut(s) 1409
Alw26I GTCTC 2 cut(s) 466, 914
Alw44I GTGCAC 1 cut(s) 1405
AlwI GGATC 2 cut(s) 777, 1382
Aor13HI TCCGGA 1 cut(s) 525
ApaI GGGCCC 2 cut(s) 506, 1052
ApaLI GTGCAC 1 cut(s) 1405
ApeKI GCWGC 5 cut(s) 1091, 1103, 1198, 1224, 1227
AsiGI ACCGGT 1 cut(s) 788
AspLEI GCGC 6 cut(s) 206, 394, 418, 937, 1135, 1198
AsuC2I CCSGG 3 cut(s) 883, 1007, 1064
AsuHPI GGTGA 7 cut(s) 61, 223, 229, 439, 618, 818, 1369
AvaII GGWCC 3 cut(s) 382, 528, 1327
BaeGI GKGCMC 4 cut(s) 506, 893, 1052, 1409
BanI GGYRCC 1 cut(s) 1364
BanII GRGCYC 4 cut(s) 9, 506, 1052, 1278
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 1409
BbvI GCAGC 5 cut(s) 1103, 1115, 1185, 1236, 1239
BccI CCATC 5 cut(s) 86, 167, 579, 715, 1084
BceAI ACGGC 8 cut(s) 212, 265, 332, 574, 679, 880, 1249, 1378
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 3 cut(s) 128, 292, 1325
BcnI CCSGG 3 cut(s) 883, 1007, 1064
BcoDI GTCTC 2 cut(s) 466, 914
BfaI CTAG 1 cut(s) 773
BfmI CTRYAG 1 cut(s) 1199
BfoI RGCGCY 2 cut(s) 419, 1199
BglII AGATCT 1 cut(s) 174
Bme1390I CCNGG 6 cut(s) 128, 292, 883, 1007, 1064, 1325
Bme18I GGWCC 3 cut(s) 382, 528, 1327
BmgBI CACGTC 4 cut(s) 61, 372, 766, 874
BmiI GGNNCC 6 cut(s) 6, 504, 860, 1050, 1051, 1366
BmrFI CCNGG 6 cut(s) 128, 292, 883, 1007, 1064, 1325
BmrI ACTGGG 2 cut(s) 280, 1386
BmsI GCATC 4 cut(s) 231, 403, 891, 1076
BmuI ACTGGG 2 cut(s) 280, 1386
BpiI GAAGAC 1 cut(s) 768
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 3 cut(s) 883, 1007, 1064
BsaAI YACGTR 1 cut(s) 1410
BsaHI GRCGYC 4 cut(s) 84, 195, 714, 1017
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 5 cut(s) 126, 291, 514, 801, 1330
BsaWI WCCGGW 3 cut(s) 525, 788, 1367
BsaXI ACNNNNNCTCC 4 cut(s) 863, 893, 1424, 1454
Bse118I RCCGGY 5 cut(s) 218, 403, 530, 686, 788
Bse1I ACTGG 2 cut(s) 286, 1392
Bse3DI GCAATG 1 cut(s) 1113
BseAI TCCGGA 1 cut(s) 525
BseBI CCWGG 3 cut(s) 128, 292, 1325
BseDI CCNNGG 5 cut(s) 126, 291, 514, 801, 1330
BseGI GGATG 3 cut(s) 63, 306, 864
BseMI GCAATG 1 cut(s) 1113
BseNI ACTGG 2 cut(s) 286, 1392
BseRI GAGGAG 3 cut(s) 8, 11, 14
BseSI GKGCMC 4 cut(s) 506, 893, 1052, 1409
BseXI GCAGC 5 cut(s) 1103, 1115, 1185, 1236, 1239
BseYI CCCAGC 1 cut(s) 1099
Bsh1236I CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
Bsh1285I CGRYCG 1 cut(s) 166
BshNI GGYRCC 1 cut(s) 1364
BshTI ACCGGT 1 cut(s) 788
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 1 cut(s) 1409
BslFI GGGAC 1 cut(s) 109
BsmAI GTCTC 2 cut(s) 466, 914
BsmFI GGGAC 1 cut(s) 109
Bso31I GGTCTC 1 cut(s) 466
Bsp120I GGGCCC 2 cut(s) 502, 1048
Bsp1286I GDGCHC 6 cut(s) 9, 506, 893, 1052, 1278, 1409
Bsp13I TCCGGA 1 cut(s) 525
Bsp143I GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
Bsp19I CCATGG 1 cut(s) 1330
BspEI TCCGGA 1 cut(s) 525
BspFNI CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
BspHI TCATGA 1 cut(s) 1308
BspLI GGNNCC 6 cut(s) 6, 504, 860, 1050, 1051, 1366
BspMAI CTGCAG 1 cut(s) 1203
BspPI GGATC 2 cut(s) 777, 1382
BspQI GCTCTTC 1 cut(s) 944
BspT107I GGYRCC 1 cut(s) 1364
BspTNI GGTCTC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 1113
BsrFI RCCGGY 5 cut(s) 218, 403, 530, 686, 788
BsrI ACTGG 2 cut(s) 286, 1392
BssAI RCCGGY 5 cut(s) 218, 403, 530, 686, 788
BssECI CCNNGG 5 cut(s) 126, 291, 514, 801, 1330
BssMI GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
BssNI GRCGYC 4 cut(s) 84, 195, 714, 1017
BssT1I CCWWGG 1 cut(s) 1330
Bst2UI CCWGG 3 cut(s) 128, 292, 1325
Bst4CI ACNGT 6 cut(s) 95, 100, 235, 241, 522, 1143
Bst6I CTCTTC 4 cut(s) 32, 38, 944, 1151
BstACI GRCGYC 4 cut(s) 84, 195, 714, 1017
BstAPI GCANNNNNTGC 1 cut(s) 400
BstBAI YACGTR 1 cut(s) 1410
BstC8I GCNNGC 8 cut(s) 55, 405, 893, 1096, 1118, 1169, 1244, 1296
BstDSI CCRYGG 2 cut(s) 801, 1330
BstEII GGTNACC 2 cut(s) 806, 1375
BstF5I GGATG 3 cut(s) 63, 306, 864
BstFNI CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
BstH2I RGCGCY 2 cut(s) 419, 1199
BstHHI GCGC 6 cut(s) 206, 394, 418, 937, 1135, 1198
BstKTI GATC 6 cut(s) 177, 390, 546, 561, 772, 1390
BstMAI GTCTC 2 cut(s) 466, 914
BstMBI GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
BstMCI CGRYCG 1 cut(s) 166
BstNI CCWGG 3 cut(s) 128, 292, 1325
BstNSI RCATGY 2 cut(s) 709, 1298
BstPI GGTNACC 2 cut(s) 806, 1375
BstSCI CCNGG 6 cut(s) 126, 290, 881, 1005, 1062, 1323
BstSFI CTRYAG 1 cut(s) 1199
BstSLI GKGCMC 4 cut(s) 506, 893, 1052, 1409
BstUI CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
BstV1I GCAGC 5 cut(s) 1103, 1115, 1185, 1236, 1239
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 2 cut(s) 174, 769
BstXI CCANNNNNNTGG 1 cut(s) 1331
BstYI RGATCY 2 cut(s) 174, 769
BtgI CCRYGG 2 cut(s) 801, 1330
BtgZI GCGATG 2 cut(s) 208, 755
BtrI CACGTC 4 cut(s) 61, 372, 766, 874
BtsCI GGATG 3 cut(s) 63, 306, 864
Cac8I GCNNGC 8 cut(s) 55, 405, 893, 1096, 1118, 1169, 1244, 1296
CciI TCATGA 1 cut(s) 1308
CfoI GCGC 6 cut(s) 206, 394, 418, 937, 1135, 1198
Cfr10I RCCGGY 5 cut(s) 218, 403, 530, 686, 788
Cfr42I CCGCGG 1 cut(s) 804
CpoI CGGWCCG 1 cut(s) 528
CseI GACGC 4 cut(s) 73, 157, 722, 734
Csp6I GTAC 6 cut(s) 96, 568, 607, 850, 1086, 1411
CspAI ACCGGT 1 cut(s) 788
CspCI CAANNNNNGTGG 2 cut(s) 1425, 1460
CspI CGGWCCG 1 cut(s) 528
CviAII CATG 5 cut(s) 691, 706, 1295, 1309, 1331
CviQI GTAC 6 cut(s) 96, 568, 607, 850, 1086, 1411
DpnI GATC 6 cut(s) 176, 389, 545, 560, 771, 1389
DpnII GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 4 cut(s) 32, 38, 944, 1151
EarI CTCTTC 4 cut(s) 32, 38, 944, 1151
EciI GGCGGA 2 cut(s) 40, 1439
Eco130I CCWWGG 1 cut(s) 1330
Eco24I GRGCYC 4 cut(s) 9, 506, 1052, 1278
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 3 cut(s) 382, 528, 1327
Eco57I CTGAAG 1 cut(s) 974
Eco91I GGTNACC 2 cut(s) 806, 1375
EcoO109I RGGNCCY 3 cut(s) 382, 1048, 1049
EcoO65I GGTNACC 2 cut(s) 806, 1375
EcoRII CCWGG 3 cut(s) 126, 290, 1323
EcoT14I CCWWGG 1 cut(s) 1330
EcoT38I GRGCYC 4 cut(s) 9, 506, 1052, 1278
ErhI CCWWGG 1 cut(s) 1330
FaeI CATG 5 cut(s) 694, 709, 1298, 1312, 1334
FaqI GGGAC 1 cut(s) 109
FatI CATG 5 cut(s) 690, 705, 1294, 1308, 1330
FauI CCCGC 7 cut(s) 62, 123, 267, 286, 332, 796, 1441
FblI GTMKAC 1 cut(s) 1148
FokI GGATG 3 cut(s) 50, 293, 851
FriOI GRGCYC 4 cut(s) 9, 506, 1052, 1278
FspBI CTAG 1 cut(s) 773
FspI TGCGCA 1 cut(s) 1134
GlaI GCGC 6 cut(s) 205, 393, 417, 936, 1134, 1197
GsaI CCCAGC 1 cut(s) 1103
HaeII RGCGCY 2 cut(s) 419, 1199
HgaI GACGC 4 cut(s) 73, 157, 722, 734
HhaI GCGC 6 cut(s) 206, 394, 418, 937, 1135, 1198
Hin1I GRCGYC 4 cut(s) 84, 195, 714, 1017
Hin1II CATG 5 cut(s) 694, 709, 1298, 1312, 1334
Hin6I GCGC 6 cut(s) 204, 392, 416, 935, 1133, 1196
HinP1I GCGC 6 cut(s) 204, 392, 416, 935, 1133, 1196
HincII GTYRAC 3 cut(s) 88, 601, 1149
HindII GTYRAC 3 cut(s) 88, 601, 1149
HinfI GANTC 2 cut(s) 342, 621
HphI GGTGA 7 cut(s) 61, 223, 229, 439, 618, 818, 1369
Hpy166II GTNNAC 5 cut(s) 88, 601, 1012, 1149, 1407
Hpy188I TCNGA 5 cut(s) 376, 518, 579, 620, 970
Hpy188III TCNNGA 7 cut(s) 145, 172, 352, 463, 526, 919, 1309
Hpy8I GTNNAC 5 cut(s) 88, 601, 1012, 1149, 1407
HpyAV CCTTC 8 cut(s) 148, 217, 325, 406, 742, 880, 1183, 1423
HpyCH4III ACNGT 6 cut(s) 95, 100, 235, 241, 522, 1143
HpyCH4V TGCA 4 cut(s) 1201, 1224, 1242, 1407
Hsp92I GRCGYC 4 cut(s) 84, 195, 714, 1017
Hsp92II CATG 5 cut(s) 694, 709, 1298, 1312, 1334
HspAI GCGC 6 cut(s) 204, 392, 416, 935, 1133, 1196
Kpn2I TCCGGA 1 cut(s) 525
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
KspI CCGCGG 1 cut(s) 804
Kzo9I GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
LguI GCTCTTC 1 cut(s) 944
LmnI GCTCC 6 cut(s) 4, 21, 279, 423, 884, 970
Lsp1109I GCAGC 5 cut(s) 1103, 1115, 1185, 1236, 1239
LweI GCATC 4 cut(s) 231, 403, 891, 1076
MaeI CTAG 1 cut(s) 773
MaeIII GTNAC 6 cut(s) 229, 235, 445, 806, 1375, 1394
MalI GATC 6 cut(s) 176, 389, 545, 560, 771, 1389
MboI GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
MflI RGATCY 2 cut(s) 174, 769
MhlI GDGCHC 6 cut(s) 9, 506, 893, 1052, 1278, 1409
MluCI AATT 1 cut(s) 976
MlyI GAGTC 1 cut(s) 615
MmeI TCCRAC 2 cut(s) 354, 948
MroI TCCGGA 1 cut(s) 525
MroNI GCCGGC 1 cut(s) 403
MseI TTAA 2 cut(s) 1347, 1521
MslI CAYNNNNRTG 3 cut(s) 689, 704, 1370
MspA1I CMGCKG 3 cut(s) 803, 1230, 1385
MspR9I CCNGG 6 cut(s) 128, 292, 883, 1007, 1064, 1325
MvaI CCWGG 3 cut(s) 128, 292, 1325
MvnI CGCG 8 cut(s) 168, 204, 392, 647, 728, 803, 842, 935
NaeI GCCGGC 1 cut(s) 405
NciI CCSGG 3 cut(s) 883, 1007, 1064
NcoI CCATGG 1 cut(s) 1330
NdeII GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
NgoMIV GCCGGC 1 cut(s) 403
NlaIII CATG 5 cut(s) 694, 709, 1298, 1312, 1334
NlaIV GGNNCC 6 cut(s) 6, 504, 860, 1050, 1051, 1366
NmeAIII GCCGAG 3 cut(s) 404, 459, 716
NmuCI GTSAC 5 cut(s) 229, 235, 445, 806, 1375
NsbI TGCGCA 1 cut(s) 1134
NspI RCATGY 2 cut(s) 709, 1298
OliI CACNNNNGTG 1 cut(s) 1370
PaeI GCATGC 1 cut(s) 1298
PagI TCATGA 1 cut(s) 1308
PciI ACATGT 1 cut(s) 705
PciSI GCTCTTC 1 cut(s) 944
PcsI WCGNNNNNNNCGW 3 cut(s) 231, 249, 506
PdiI GCCGGC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 342
PflFI GACNNNGTC 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 881
PinAI ACCGGT 1 cut(s) 788
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Ppu21I YACGTR 1 cut(s) 1410
PpuMI RGGWCCY 1 cut(s) 382
PscI ACATGT 1 cut(s) 705
Psp5II RGGWCCY 1 cut(s) 382
Psp6I CCWGG 3 cut(s) 126, 290, 1323
PspEI GGTNACC 2 cut(s) 806, 1375
PspFI CCCAGC 1 cut(s) 1099
PspGI CCWGG 3 cut(s) 126, 290, 1323
PspN4I GGNNCC 6 cut(s) 6, 504, 860, 1050, 1051, 1366
PspOMI GGGCCC 2 cut(s) 502, 1048
PspPPI RGGWCCY 1 cut(s) 382
PsrI GAACNNNNNNTAC 4 cut(s) 1199, 1231, 1304, 1336
PstI CTGCAG 1 cut(s) 1203
PsuI RGATCY 2 cut(s) 174, 769
PsyI GACNNNGTC 1 cut(s) 167
RsaI GTAC 6 cut(s) 97, 569, 608, 851, 1087, 1412
RsaNI GTAC 6 cut(s) 96, 568, 607, 850, 1086, 1411
RseI CAYNNNNRTG 3 cut(s) 689, 704, 1370
Rsr2I CGGWCCG 1 cut(s) 528
RsrII CGGWCCG 1 cut(s) 528
SacII CCGCGG 1 cut(s) 804
SalI GTCGAC 1 cut(s) 1147
SapI GCTCTTC 1 cut(s) 944
SaqAI TTAA 2 cut(s) 1347, 1521
Sau3AI GATC 6 cut(s) 174, 387, 543, 558, 769, 1387
SchI GAGTC 1 cut(s) 615
ScrFI CCNGG 6 cut(s) 128, 292, 883, 1007, 1064, 1325
SduI GDGCHC 6 cut(s) 9, 506, 893, 1052, 1278, 1409
SfaNI GCATC 4 cut(s) 231, 403, 891, 1076
SfcI CTRYAG 1 cut(s) 1199
Sfr303I CCGCGG 1 cut(s) 804
SgrBI CCGCGG 1 cut(s) 804
SgrDI CGTCGACG 1 cut(s) 1147
SinI GGWCC 3 cut(s) 382, 528, 1327
SmiMI CAYNNNNRTG 3 cut(s) 689, 704, 1370
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
SphI GCATGC 1 cut(s) 1298
Sse9I AATT 1 cut(s) 976
SspI AATATT 1 cut(s) 1525
SspMI CTAG 1 cut(s) 773
StyD4I CCNGG 6 cut(s) 126, 290, 881, 1005, 1062, 1323
StyI CCWWGG 1 cut(s) 1330
TaaI ACNGT 6 cut(s) 95, 100, 235, 241, 522, 1143
TaqI TCGA 9 cut(s) 162, 180, 464, 552, 675, 711, 918, 1002, 1148
TasI AATT 1 cut(s) 976
TauI GCSGC 5 cut(s) 77, 284, 669, 1196, 1301
TfiI GAWTC 1 cut(s) 342
Tru1I TTAA 2 cut(s) 1347, 1521
Tru9I TTAA 2 cut(s) 1347, 1521
TseFI GTSAC 5 cut(s) 229, 235, 445, 806, 1375
TseI GCWGC 5 cut(s) 1091, 1103, 1198, 1224, 1227
Tsp45I GTSAC 5 cut(s) 229, 235, 445, 806, 1375
TspDTI ATGAA 6 cut(s) 425, 707, 758, 774, 1301, 1325
TspGWI ACGGA 2 cut(s) 111, 1050
Tth111I GACNNNGTC 1 cut(s) 167
VneI GTGCAC 1 cut(s) 1405
VpaK11BI GGWCC 3 cut(s) 382, 528, 1327
XceI RCATGY 2 cut(s) 709, 1298
XmiI GTMKAC 1 cut(s) 1148
XspI CTAG 1 cut(s) 773
ZraI GACGTC 2 cut(s) 196, 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.