FvH4_3g37320

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015583
Physical Location & Seq
Reverse (-)
32028665 .. 32029096
432 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37320.t1

Sequence Viewer

Length: 432 bp
ATGTCAATCATCCCAATCAACGAGCGACGAAGCAGTAGTGACTTCGATCCCTGGTCTCTCGACCTGTGGGACCCATTCCTCGACTTCCCTCTCTTCCCTTCTTCACTCTCCAACGTCTTCCACCAACTCAATCCCGGGTTTTCCGCAGTGAACTCCCGCCTCGATTGGAGCGAGACCCGAAACGCTCACGTTCTCAAAGCAGCTCTTCCTGCTTTTGCAAACGAGGACGTGCTTGTTGAGCTTCAAGAGGAGCGAGTGCTTCAGATTAGCACAGACAGCGGTGGCTTCATGACCAAGGTTAAGCTCCCTGAGAATGCCAAGACTGAGGAGCTCAAGGCCTTTATGAGCAATGGGGTTGTTACTGTCACTGTTCCTAAGGTGGAGGCTAGGACACCCAATGTGAGAGCCATTGAAATTTCTGGTGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

144

Amino Acids

15.96

Weight (kDa)

4.73

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 55 - 140 8.9e-13 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015583)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37320
malus_domestica MD03G1081800.v1.1
prunus_persica Prupe.6G008800_v2.0.a1
pyrus_communis pycom03g06520
rosa_chinensis RchiOBHm_Chr5g0067101
rosa_laevigata RLG00000035910 RLG00000035913
rosa_multiflora Rmu_sc0000151.1_g000011
rosa_roxburghii Rroxscaffold_1G00013970
rosa_rugosa Rorug05G0381500 Rorug05G0381800
rosa_samantha Rh5AG441800 Rh5BG458800 Rh5CG481000 Rh5DG473700
rosa_wichuraiana Rw5G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 144, 157, 279
AclWI GGATC 1 cut(s) 41
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 245
AgsI TTSAA 2 cut(s) 245, 413
AjiI CACGTC 1 cut(s) 229
AjnI CCWGG 1 cut(s) 50
AluBI AGCT 4 cut(s) 203, 241, 304, 331
AluI AGCT 4 cut(s) 203, 241, 304, 331
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 2 cut(s) 60, 167
AlwI GGATC 1 cut(s) 41
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 414
AspS9I GGNCC 1 cut(s) 70
AsuC2I CCSGG 2 cut(s) 135, 136
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 70
AxyI CCTNAGG 1 cut(s) 375
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 212
BciT130I CCWGG 1 cut(s) 52
BcnI CCSGG 2 cut(s) 135, 136
BcoDI GTCTC 2 cut(s) 60, 167
BfaI CTAG 1 cut(s) 387
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 3 cut(s) 52, 135, 136
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 229
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 71, 72
BmrFI CCNGG 3 cut(s) 52, 135, 136
BpiI GAAGAC 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 2 cut(s) 135, 136
BsaI GGTCTC 2 cut(s) 60, 167
BsaJI CCNNGG 3 cut(s) 50, 134, 294
Bse21I CCTNAGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 355
BseBI CCWGG 1 cut(s) 52
BseDI CCNNGG 3 cut(s) 50, 134, 294
BseGI GGATG 1 cut(s) 9
BseMI GCAATG 1 cut(s) 355
BseMII CTCAG 2 cut(s) 300, 315
BseRI GAGGAG 2 cut(s) 263, 341
BseXI GCAGC 1 cut(s) 212
BshFI GGCC 1 cut(s) 338
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 1 cut(s) 134
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 60, 167
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 1 cut(s) 338
Bso31I GGTCTC 2 cut(s) 60, 167
BsoBI CYCGRG 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 333
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 3 cut(s) 144, 157, 279
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 2 cut(s) 301, 316
BspHI TCATGA 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 71, 72
BspPI GGATC 1 cut(s) 41
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 2 cut(s) 60, 167
BsrDI GCAATG 1 cut(s) 355
BssECI CCNNGG 3 cut(s) 50, 134, 294
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 52
Bst4CI ACNGT 2 cut(s) 364, 370
Bst6I CTCTTC 2 cut(s) 98, 210
BstDEI CTNAG 3 cut(s) 309, 324, 375
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 2 cut(s) 60, 167
BstMBI GATC 1 cut(s) 46
BstMWI GCNNNNNNNGC 3 cut(s) 209, 238, 276
BstNI CCWGG 1 cut(s) 52
BstSCI CCNGG 3 cut(s) 50, 133, 134
BstV1I GCAGC 1 cut(s) 212
BstV2I GAAGAC 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 1 cut(s) 338
BtrI CACGTC 1 cut(s) 229
BtsCI GGATG 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 153
BtsIMutI CAGTG 2 cut(s) 153, 366
CciI TCATGA 1 cut(s) 288
Cfr13I GGNCC 1 cut(s) 70
Cfr9I CCCGGG 1 cut(s) 134
CviAII CATG 1 cut(s) 289
CviJI RGCY 8 cut(s) 203, 241, 285, 304, 331, 338, 386, 407
CviKI_1 RGCY 8 cut(s) 203, 241, 285, 304, 331, 338, 386, 407
DdeI CTNAG 3 cut(s) 309, 324, 375
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
Eam1104I CTCTTC 2 cut(s) 98, 210
EarI CTCTTC 2 cut(s) 98, 210
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 1 cut(s) 294
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 2 cut(s) 60, 167
Eco47I GGWCC 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 1 cut(s) 245
Eco81I CCTNAGG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 134
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 1 cut(s) 50
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 1 cut(s) 292
FaiI YATR 2 cut(s) 290, 344
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FaqI GGGAC 1 cut(s) 83
FatI CATG 1 cut(s) 288
FauI CCCGC 1 cut(s) 164
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 1 cut(s) 387
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 338
HapII CCGG 1 cut(s) 135
Hin1II CATG 1 cut(s) 292
HpaII CCGG 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 151
Hpy188I TCNGA 1 cut(s) 264
Hpy188III TCNNGA 3 cut(s) 59, 245, 289
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 108
HpyCH4III ACNGT 2 cut(s) 364, 370
HpyCH4IV ACGT 3 cut(s) 114, 189, 228
HpyCH4V TGCA 1 cut(s) 218
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 238, 276
HpyF3I CTNAG 3 cut(s) 309, 324, 375
HpySE526I ACGT 3 cut(s) 114, 189, 228
Hsp92II CATG 1 cut(s) 292
KflI GGGWCCC 1 cut(s) 70
Kzo9I GATC 1 cut(s) 46
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 4 cut(s) 168, 250, 309, 328
LpnPI CCDG 7 cut(s) 37, 64, 77, 148, 222, 321, 405
Lsp1109I GCAGC 1 cut(s) 212
MaeI CTAG 1 cut(s) 387
MaeII ACGT 3 cut(s) 114, 189, 228
MaeIII GTNAC 3 cut(s) 38, 358, 364
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 4 cut(s) 85, 93, 109, 197
MhlI GDGCHC 1 cut(s) 333
MluCI AATT 1 cut(s) 414
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 7 cut(s) 89, 99, 170, 217, 241, 319, 376
MseI TTAA 1 cut(s) 300
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 3 cut(s) 52, 135, 136
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 1 cut(s) 52
MwoI GCNNNNNNNGC 3 cut(s) 209, 238, 276
NciI CCSGG 2 cut(s) 135, 136
NdeII GATC 1 cut(s) 46
NlaIII CATG 1 cut(s) 292
NlaIV GGNNCC 2 cut(s) 71, 72
NmuCI GTSAC 2 cut(s) 38, 364
PagI TCATGA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 319
PkrI GCNGC 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 70
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 50
PspGI CCWGG 1 cut(s) 50
PspN4I GGNNCC 2 cut(s) 71, 72
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 70
ScrFI CCNGG 3 cut(s) 52, 135, 136
SduI GDGCHC 1 cut(s) 333
SinI GGWCC 1 cut(s) 70
SmaI CCCGGG 1 cut(s) 136
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 1 cut(s) 414
SseBI AGGCCT 1 cut(s) 338
SsiI CCGC 3 cut(s) 144, 157, 279
SspMI CTAG 1 cut(s) 387
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 338
StyD4I CCNGG 3 cut(s) 50, 133, 134
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 2 cut(s) 364, 370
TaiI ACGT 3 cut(s) 117, 192, 231
TaqI TCGA 4 cut(s) 45, 60, 81, 162
TasI AATT 1 cut(s) 414
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TscAI CASTG 2 cut(s) 153, 373
TseFI GTSAC 2 cut(s) 38, 364
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 2 cut(s) 38, 364
TspDTI ATGAA 1 cut(s) 277
TspMI CCCGGG 1 cut(s) 134
TspRI CASTG 2 cut(s) 153, 373
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 1 cut(s) 414
XmaI CCCGGG 1 cut(s) 134
XspI CTAG 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.