Rroxscaffold_1G00013970

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015583
Physical Location & Seq
Forward (+)
17250491 .. 17250919
429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013970.1

Sequence Viewer

Length: 429 bp
ATGTCAATCATCCCAATCAACGAGCGACGAAGCAGTGACGTCGATCCCTGGGCTCTCGACCTCTGGGACCCATTCCTGGATTTCCCATTATTCCCTTCTTCACTCTCCAACGTTTTCCATGAACTCAATCCGGGTTTTCCCTCGGTGAATTCCCGGCTCGATTGGAGCGAGACCCGCAACGTCCACGTTCTTAAGGCAGCTCTTCCTGCGTTTGCCAACGAAGACGTGCTTGTTGAGCTTCAAGAAGCCCGAGTGCTTCAGATTAGCACAGACAGCGGTAGCTTCATGACCAAGATTAAGCTCCCGGAGAACGCCAAGATTGAGGAGCTCAAGGCCTTTATGAGCAATGGGGTTGTTACTGTCACTGTTCCCAAGGGGGAGGCTGAGAGGCCCAATGTCAGAGCCATTGAAATTTCTGGCGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

142

Amino Acids

15.85

Weight (kDa)

4.67

Isoelectric Point (pI)

56.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 54 - 139 2.1e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015583)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37320
malus_domestica MD03G1081800.v1.1
prunus_persica Prupe.6G008800_v2.0.a1
pyrus_communis pycom03g06520
rosa_chinensis RchiOBHm_Chr5g0067101
rosa_laevigata RLG00000035910 RLG00000035913
rosa_multiflora Rmu_sc0000151.1_g000011
rosa_roxburghii Rroxscaffold_1G00013970
rosa_rugosa Rorug05G0381500 Rorug05G0381800
rosa_samantha Rh5AG441800 Rh5BG458800 Rh5CG481000 Rh5DG473700
rosa_wichuraiana Rw5G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AciI CCGC 2 cut(s) 175, 276
AclI AACGTT 1 cut(s) 111
AclWI GGATC 1 cut(s) 38
AcsI RAATTY 2 cut(s) 148, 411
AcuI CTGAAG 1 cut(s) 242
AcyI GRCGYC 1 cut(s) 39
AfiI CCNNNNNNNGG 1 cut(s) 76
AflII CTTAAG 1 cut(s) 191
AgsI TTSAA 2 cut(s) 242, 410
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 2 cut(s) 47, 75
AluBI AGCT 5 cut(s) 200, 238, 282, 301, 328
AluI AGCT 5 cut(s) 200, 238, 282, 301, 328
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 249
AoxI GGCC 2 cut(s) 333, 389
ApeKI GCWGC 1 cut(s) 197
ApoI RAATTY 2 cut(s) 148, 411
AspS9I GGNCC 2 cut(s) 67, 390
AsuC2I CCSGG 3 cut(s) 132, 154, 305
AsuHPI GGTGA 1 cut(s) 157
AvaI CYCGRG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 67
BanII GRGCYC 2 cut(s) 55, 330
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 1 cut(s) 209
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 2 cut(s) 49, 77
BcnI CCSGG 3 cut(s) 132, 154, 305
BcoDI GTCTC 1 cut(s) 164
BfrI CTTAAG 1 cut(s) 191
BisI GCNGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 199
Bme1390I CCNGG 5 cut(s) 49, 77, 132, 154, 305
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 249
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 2 cut(s) 67, 390
BmiI GGNNCC 2 cut(s) 68, 69
BmrFI CCNGG 5 cut(s) 49, 77, 132, 154, 305
BpiI GAAGAC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 314
BpuMI CCSGG 3 cut(s) 132, 154, 305
BsaHI GRCGYC 1 cut(s) 39
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 4 cut(s) 47, 48, 141, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 2 cut(s) 49, 77
BseDI CCNNGG 4 cut(s) 47, 48, 141, 372
BseGI GGATG 1 cut(s) 9
BseLI CCNNNNNNNGG 1 cut(s) 76
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 1 cut(s) 375
BseRI GAGGAG 1 cut(s) 338
BseXI GCAGC 1 cut(s) 209
BshFI GGCC 2 cut(s) 335, 391
BsiHKAI GWGCWC 1 cut(s) 330
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 3 cut(s) 131, 154, 305
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 164
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 2 cut(s) 335, 391
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 249
Bsp1286I GDGCHC 2 cut(s) 55, 330
Bsp143I GATC 1 cut(s) 43
BspACI CCGC 2 cut(s) 175, 276
BspANI GGCC 2 cut(s) 335, 391
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 2 cut(s) 68, 69
BspPI GGATC 1 cut(s) 38
BspQI GCTCTTC 1 cut(s) 207
BspTI CTTAAG 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 352
BssECI CCNNGG 4 cut(s) 47, 48, 141, 372
BssMI GATC 1 cut(s) 43
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 49, 77
Bst4CI ACNGT 2 cut(s) 361, 367
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 39
BstAFI CTTAAG 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 9
BstKTI GATC 1 cut(s) 46
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 43
BstMWI GCNNNNNNNGC 4 cut(s) 174, 206, 235, 273
BstNI CCWGG 2 cut(s) 49, 77
BstSCI CCNGG 5 cut(s) 47, 75, 130, 152, 303
BstV1I GCAGC 1 cut(s) 209
BstV2I GAAGAC 1 cut(s) 228
BsuRI GGCC 2 cut(s) 335, 391
BtrI CACGTC 1 cut(s) 226
BtsCI GGATG 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 40, 363
CciI TCATGA 1 cut(s) 285
Cfr13I GGNCC 2 cut(s) 67, 390
CviAII CATG 2 cut(s) 119, 286
DdeI CTNAG 1 cut(s) 384
DpnI GATC 1 cut(s) 45
DpnII GATC 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 372
Eco147I AGGCCT 1 cut(s) 335
Eco24I GRGCYC 2 cut(s) 55, 330
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 67
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 249
EcoICRI GAGCTC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 2 cut(s) 47, 75
EcoT14I CCWWGG 1 cut(s) 372
EcoT38I GRGCYC 2 cut(s) 55, 330
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 2 cut(s) 122, 289
FaiI YATR 3 cut(s) 120, 287, 341
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 80
FatI CATG 2 cut(s) 118, 285
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 198
FriOI GRGCYC 2 cut(s) 55, 330
Fsp4HI GCNGC 1 cut(s) 198
GluI GCNGC 1 cut(s) 198
HaeIII GGCC 2 cut(s) 335, 391
HapII CCGG 3 cut(s) 131, 154, 305
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 2 cut(s) 122, 289
HpaII CCGG 3 cut(s) 131, 154, 305
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 261, 401
Hpy188III TCNNGA 3 cut(s) 56, 242, 286
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 2 cut(s) 30, 44
HpyAV CCTTC 1 cut(s) 105
HpyCH4III ACNGT 2 cut(s) 361, 367
HpyCH4IV ACGT 5 cut(s) 39, 111, 180, 186, 225
HpyF10VI GCNNNNNNNGC 4 cut(s) 174, 206, 235, 273
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 5 cut(s) 39, 111, 180, 186, 225
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 2 cut(s) 122, 289
KflI GGGWCCC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 43
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 3 cut(s) 165, 306, 325
Lsp1109I GCAGC 1 cut(s) 209
MaeII ACGT 5 cut(s) 39, 111, 180, 186, 225
MaeIII GTNAC 3 cut(s) 35, 355, 361
MalI GATC 1 cut(s) 45
MboI GATC 1 cut(s) 43
MboII GAAGA 3 cut(s) 90, 194, 233
MhlI GDGCHC 2 cut(s) 55, 330
MluCI AATT 2 cut(s) 148, 411
MmeI TCCRAC 1 cut(s) 132
MnlI CCTC 5 cut(s) 71, 151, 316, 373, 381
MseI TTAA 3 cut(s) 192, 297, 427
MspA1I CMGCKG 1 cut(s) 276
MspCI CTTAAG 1 cut(s) 191
MspI CCGG 3 cut(s) 131, 154, 305
MspR9I CCNGG 5 cut(s) 49, 77, 132, 154, 305
MvaI CCWGG 2 cut(s) 49, 77
MwoI GCNNNNNNNGC 4 cut(s) 174, 206, 235, 273
NciI CCSGG 3 cut(s) 132, 154, 305
NdeII GATC 1 cut(s) 43
NlaIII CATG 2 cut(s) 122, 289
NlaIV GGNNCC 2 cut(s) 68, 69
NmuCI GTSAC 2 cut(s) 35, 361
PagI TCATGA 1 cut(s) 285
PasI CCCWGGG 1 cut(s) 48
PceI AGGCCT 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PfoI TCCNGGA 2 cut(s) 75, 303
PkrI GCNGC 1 cut(s) 199
PpuMI RGGWCCY 1 cut(s) 67
Psp124BI GAGCTC 1 cut(s) 330
Psp1406I AACGTT 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 67
Psp6I CCWGG 2 cut(s) 47, 75
PspGI CCWGG 2 cut(s) 47, 75
PspN4I GGNNCC 2 cut(s) 68, 69
PspPI GGNCC 2 cut(s) 67, 390
PspPPI RGGWCCY 1 cut(s) 67
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 3 cut(s) 192, 297, 427
SatI GCNGC 1 cut(s) 198
Sau3AI GATC 1 cut(s) 43
Sau96I GGNCC 2 cut(s) 67, 390
ScrFI CCNGG 5 cut(s) 49, 77, 132, 154, 305
SduI GDGCHC 2 cut(s) 55, 330
SinI GGWCC 1 cut(s) 67
SmlI CTYRAG 2 cut(s) 191, 329
SmoI CTYRAG 2 cut(s) 191, 329
Sse9I AATT 2 cut(s) 148, 411
SseBI AGGCCT 1 cut(s) 335
SsiI CCGC 2 cut(s) 175, 276
SstI GAGCTC 1 cut(s) 330
StuI AGGCCT 1 cut(s) 335
StyD4I CCNGG 5 cut(s) 47, 75, 130, 152, 303
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 2 cut(s) 361, 367
TaiI ACGT 5 cut(s) 42, 114, 183, 189, 228
TaqI TCGA 3 cut(s) 42, 57, 159
TasI AATT 2 cut(s) 148, 411
Tru1I TTAA 3 cut(s) 192, 297, 427
Tru9I TTAA 3 cut(s) 192, 297, 427
TscAI CASTG 2 cut(s) 40, 370
TseFI GTSAC 2 cut(s) 35, 361
TseI GCWGC 1 cut(s) 197
Tsp45I GTSAC 2 cut(s) 35, 361
TspDTI ATGAA 2 cut(s) 135, 274
TspRI CASTG 2 cut(s) 40, 370
Vha464I CTTAAG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 2 cut(s) 148, 411
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.