pycom03g06520

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0015583
Physical Location & Seq
Forward (+)
5094625 .. 5095077
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06520.1

Sequence Viewer

Length: 453 bp
ATGTCGATCGTCCCAATCAACGAGCGACGAGGCAGCGACGACTTCGACAATCTCTCATCGGCCCTCGACCTGTGGGACCCATTCACGGATTTCCCATTCCGAGCATTTCCATTCCATTCCCCAATCTCCAACGTCCTCCGGGAATTCAATCCGTTTTCTTCCGTAAACTCCCGGCTCGACTGGAGAGAGACCCGAAATGCCCACGTCCTGACCGCGGCGCTGCCGGCGTTCGCCAACGAGGAAGTGCTCGTTTCGCTTGAGGACGAACGAGTGCTCCAGATCAGCACCGACAGCGGCAGCTTCACGACGAGGATGAAGCTGCCGGACGACGCCAGGATCGAGGAGCTCAAGGCGTTCATGAGCAATGGAGTGCTCACTGTGACTGTTCCTAAGTTGGCGGCCGCCGGCGGCGGGCGCGATGTCAGGGTTATTGAAATTTCCGGCGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

151

Amino Acids

16.69

Weight (kDa)

4.65

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 60 - 146 8.5e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015583)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37320
malus_domestica MD03G1081800.v1.1
prunus_persica Prupe.6G008800_v2.0.a1
pyrus_communis pycom03g06520
rosa_chinensis RchiOBHm_Chr5g0067101
rosa_laevigata RLG00000035910 RLG00000035913
rosa_multiflora Rmu_sc0000151.1_g000011
rosa_roxburghii Rroxscaffold_1G00013970
rosa_rugosa Rorug05G0381500 Rorug05G0381800
rosa_samantha Rh5AG441800 Rh5BG458800 Rh5CG481000 Rh5DG473700
rosa_wichuraiana Rw5G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 215, 417
AciI CCGC 7 cut(s) 213, 215, 294, 398, 402, 408, 411
AclWI GGATC 1 cut(s) 344
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 2 cut(s) 143, 435
AcyI GRCGYC 1 cut(s) 330
AfiI CCNNNNNNNGG 3 cut(s) 85, 214, 411
AgsI TTSAA 2 cut(s) 148, 434
AjiI CACGTC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 332
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 3 cut(s) 300, 319, 346
AluI AGCT 3 cut(s) 300, 319, 346
Alw21I GWGCWC 4 cut(s) 249, 276, 348, 375
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 1 cut(s) 344
AoxI GGCC 2 cut(s) 60, 399
ApeKI GCWGC 4 cut(s) 33, 220, 297, 319
ApoI RAATTY 2 cut(s) 143, 435
AspLEI GCGC 2 cut(s) 220, 417
AspS9I GGNCC 2 cut(s) 61, 76
AsuC2I CCSGG 2 cut(s) 140, 172
AvaII GGWCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 348
Bbv12I GWGCWC 4 cut(s) 249, 276, 348, 375
BbvI GCAGC 4 cut(s) 45, 207, 306, 309
BciT130I CCWGG 1 cut(s) 334
BcnI CCSGG 2 cut(s) 140, 172
BcoDI GTCTC 1 cut(s) 182
BfoI RGCGCY 1 cut(s) 221
BisI GCNGC 9 cut(s) 34, 216, 221, 295, 298, 320, 399, 402, 409
BlsI GCNGC 9 cut(s) 35, 217, 222, 296, 299, 321, 400, 403, 410
Bme1390I CCNGG 3 cut(s) 140, 172, 334
Bme18I GGWCC 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 205
BmgT120I GGNCC 2 cut(s) 61, 76
BmiI GGNNCC 2 cut(s) 77, 78
BmrFI CCNGG 3 cut(s) 140, 172, 334
BpmI CTGGAG 2 cut(s) 202, 260
BpuEI CTTGAG 2 cut(s) 278, 332
BpuMI CCSGG 2 cut(s) 140, 172
BsaHI GRCGYC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 214, 411
Bse118I RCCGGY 2 cut(s) 223, 404
Bse1I ACTGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 1 cut(s) 318
BseLI CCNNNNNNNGG 3 cut(s) 85, 214, 411
BseMI GCAATG 1 cut(s) 370
BseNI ACTGG 1 cut(s) 185
BseRI GAGGAG 1 cut(s) 356
BseX3I CGGCCG 1 cut(s) 399
BseXI GCAGC 4 cut(s) 45, 207, 306, 309
Bsh1236I CGCG 2 cut(s) 215, 417
Bsh1285I CGRYCG 2 cut(s) 9, 402
BshFI GGCC 2 cut(s) 62, 401
BsiEI CGRYCG 2 cut(s) 9, 402
BsiHKAI GWGCWC 4 cut(s) 249, 276, 348, 375
BsiSI CCGG 6 cut(s) 139, 172, 224, 323, 405, 441
BslFI GGGAC 1 cut(s) 89
BslI CCNNNNNNNGG 3 cut(s) 85, 214, 411
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 1 cut(s) 89
BsnI GGCC 2 cut(s) 62, 401
Bso31I GGTCTC 1 cut(s) 182
Bsp1286I GDGCHC 4 cut(s) 249, 276, 348, 375
Bsp143I GATC 3 cut(s) 6, 279, 336
BspACI CCGC 7 cut(s) 213, 215, 294, 398, 402, 408, 411
BspANI GGCC 2 cut(s) 62, 401
BspFNI CGCG 2 cut(s) 215, 417
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 2 cut(s) 77, 78
BspPI GGATC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 370
BsrFI RCCGGY 2 cut(s) 223, 404
BsrI ACTGG 1 cut(s) 185
BssAI RCCGGY 2 cut(s) 223, 404
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 3 cut(s) 6, 279, 336
BssNI GRCGYC 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 334
Bst4CI ACNGT 2 cut(s) 379, 385
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 3 cut(s) 225, 406, 413
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 213
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 2 cut(s) 215, 417
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 2 cut(s) 220, 417
BstKTI GATC 3 cut(s) 9, 282, 339
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 3 cut(s) 6, 279, 336
BstMCI CGRYCG 2 cut(s) 9, 402
BstMWI GCNNNNNNNGC 4 cut(s) 224, 253, 291, 414
BstNI CCWGG 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 138, 170, 332
BstUI CGCG 2 cut(s) 215, 417
BstV1I GCAGC 4 cut(s) 45, 207, 306, 309
BstZI CGGCCG 1 cut(s) 399
BsuRI GGCC 2 cut(s) 62, 401
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 432
BtrI CACGTC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 3 cut(s) 225, 406, 413
CciI TCATGA 1 cut(s) 357
CciNI GCGGCCGC 1 cut(s) 399
CfoI GCGC 2 cut(s) 220, 417
Cfr10I RCCGGY 2 cut(s) 223, 404
Cfr13I GGNCC 2 cut(s) 61, 76
Cfr42I CCGCGG 1 cut(s) 216
CseI GACGC 1 cut(s) 338
CviAII CATG 1 cut(s) 358
CviJI RGCY 6 cut(s) 62, 175, 300, 319, 346, 401
CviKI_1 RGCY 6 cut(s) 62, 175, 300, 319, 346, 401
DdeI CTNAG 1 cut(s) 390
DpnI GATC 3 cut(s) 8, 281, 338
DpnII GATC 3 cut(s) 6, 279, 336
EaeI YGGCCR 1 cut(s) 399
EagI CGGCCG 1 cut(s) 399
Ecl136II GAGCTC 1 cut(s) 346
EclXI CGGCCG 1 cut(s) 399
Eco24I GRGCYC 1 cut(s) 348
Eco31I GGTCTC 1 cut(s) 182
Eco47I GGWCC 1 cut(s) 76
Eco52I CGGCCG 1 cut(s) 399
Eco53kI GAGCTC 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 76
EcoRI GAATTC 1 cut(s) 143
EcoRII CCWGG 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 1 cut(s) 361
FaiI YATR 1 cut(s) 359
FaqI GGGAC 1 cut(s) 89
FatI CATG 1 cut(s) 357
FauI CCCGC 1 cut(s) 404
Fnu4HI GCNGC 9 cut(s) 34, 216, 221, 295, 298, 320, 399, 402, 409
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 9 cut(s) 34, 216, 221, 295, 298, 320, 399, 402, 409
GlaI GCGC 2 cut(s) 219, 416
GluI GCNGC 9 cut(s) 34, 216, 221, 295, 298, 320, 399, 402, 409
GsuI CTGGAG 2 cut(s) 202, 260
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 2 cut(s) 62, 401
HapII CCGG 6 cut(s) 139, 172, 224, 323, 405, 441
HgaI GACGC 1 cut(s) 338
HhaI GCGC 2 cut(s) 220, 417
Hin1I GRCGYC 1 cut(s) 330
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 2 cut(s) 218, 415
HinP1I GCGC 2 cut(s) 218, 415
HpaII CCGG 6 cut(s) 139, 172, 224, 323, 405, 441
Hpy166II GTNNAC 1 cut(s) 166
Hpy188I TCNGA 1 cut(s) 101
Hpy188III TCNNGA 4 cut(s) 208, 277, 304, 358
Hpy8I GTNNAC 1 cut(s) 166
Hpy99I CGWCG 4 cut(s) 30, 41, 310, 332
HpyCH4III ACNGT 2 cut(s) 379, 385
HpyCH4IV ACGT 2 cut(s) 132, 204
HpyF10VI GCNNNNNNNGC 4 cut(s) 224, 253, 291, 414
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 2 cut(s) 132, 204
Hsp92I GRCGYC 1 cut(s) 330
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 2 cut(s) 218, 415
KflI GGGWCCC 1 cut(s) 76
KroI GCCGGC 2 cut(s) 223, 404
KroNI GCCGGC 2 cut(s) 225, 406
KspI CCGCGG 1 cut(s) 216
Kzo9I GATC 3 cut(s) 6, 279, 336
LmnI GCTCC 2 cut(s) 279, 343
Lsp1109I GCAGC 4 cut(s) 45, 207, 306, 309
MaeII ACGT 2 cut(s) 132, 204
MaeIII GTNAC 1 cut(s) 379
MalI GATC 3 cut(s) 8, 281, 338
MboI GATC 3 cut(s) 6, 279, 336
MboII GAAGA 1 cut(s) 150
MhlI GDGCHC 4 cut(s) 249, 276, 348, 375
MluCI AATT 2 cut(s) 143, 435
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 7 cut(s) 23, 74, 146, 232, 253, 303, 334
MreI CGCCGGCG 1 cut(s) 404
MroNI GCCGGC 2 cut(s) 223, 404
MspA1I CMGCKG 2 cut(s) 215, 294
MspI CCGG 6 cut(s) 139, 172, 224, 323, 405, 441
MspR9I CCNGG 3 cut(s) 140, 172, 334
MvaI CCWGG 1 cut(s) 334
MvnI CGCG 2 cut(s) 215, 417
MwoI GCNNNNNNNGC 4 cut(s) 224, 253, 291, 414
NaeI GCCGGC 2 cut(s) 225, 406
NciI CCSGG 2 cut(s) 140, 172
NdeII GATC 3 cut(s) 6, 279, 336
NgoMIV GCCGGC 2 cut(s) 223, 404
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 2 cut(s) 77, 78
NmuCI GTSAC 1 cut(s) 379
NotI GCGGCCGC 1 cut(s) 399
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdiI GCCGGC 2 cut(s) 225, 406
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 9 cut(s) 35, 217, 222, 296, 299, 321, 400, 403, 410
Ple19I CGATCG 1 cut(s) 9
PpuMI RGGWCCY 1 cut(s) 76
Psp124BI GAGCTC 1 cut(s) 348
Psp5II RGGWCCY 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 332
PspGI CCWGG 1 cut(s) 332
PspN4I GGNNCC 2 cut(s) 77, 78
PspPI GGNCC 2 cut(s) 61, 76
PspPPI RGGWCCY 1 cut(s) 76
PvuI CGATCG 1 cut(s) 9
SacI GAGCTC 1 cut(s) 348
SacII CCGCGG 1 cut(s) 216
SatI GCNGC 9 cut(s) 34, 216, 221, 295, 298, 320, 399, 402, 409
Sau3AI GATC 3 cut(s) 6, 279, 336
Sau96I GGNCC 2 cut(s) 61, 76
ScrFI CCNGG 3 cut(s) 140, 172, 334
SduI GDGCHC 4 cut(s) 249, 276, 348, 375
SetI ASST 6 cut(s) 72, 135, 207, 302, 321, 348
Sfr303I CCGCGG 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 404
SgrBI CCGCGG 1 cut(s) 216
SinI GGWCC 1 cut(s) 76
SmlI CTYRAG 2 cut(s) 257, 347
SmoI CTYRAG 2 cut(s) 257, 347
Sse9I AATT 2 cut(s) 143, 435
SsiI CCGC 7 cut(s) 213, 215, 294, 398, 402, 408, 411
SstI GAGCTC 1 cut(s) 348
StyD4I CCNGG 3 cut(s) 138, 170, 332
TaaI ACNGT 2 cut(s) 379, 385
TaiI ACGT 2 cut(s) 135, 207
TaqI TCGA 5 cut(s) 5, 45, 66, 177, 339
TasI AATT 2 cut(s) 143, 435
TauI GCSGC 5 cut(s) 218, 297, 401, 404, 411
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 379
TseI GCWGC 4 cut(s) 33, 220, 297, 319
Tsp45I GTSAC 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 329, 346
TspGWI ACGGA 3 cut(s) 101, 141, 151
TspRI CASTG 1 cut(s) 382
VpaK11BI GGWCC 1 cut(s) 76
XapI RAATTY 2 cut(s) 143, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.