Rorug05G0381800

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0015583
Physical Location & Seq
Reverse (-)
51758524 .. 51759998
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0381800.1

Sequence Viewer

Length: 621 bp
ATGGCGGACTCGGACAACGACTCGGGAGGCGGGCACAACAGCCCGGGAGGGTATACGAAGAGCGAGCTGTCACCTCGGGAACAGGACCGGCTGTTACCAATTGCCAACGTCAGCAGGATCATGAAGAAGGCGCTGCCGGCCAACGCCAAGATCTCCAAGGACGCCAAGGAGACCGTGCAGGAGTGCGTCTCCGAGTTCATAAGCTTCATCACCGGCGAGGCCTCCGACAAGTGTCAGCGCGAGAAGCGGAAGACCATCAACGGCGACGATTTGCTCTGGGCCATGACCACCCTCGGCTTCGAGGAGTACGTCGAGCCGCTCAAGGTGTACTTGCAGCGGTTTAGGGAGATGGAGGGGGAGAAGAGTAGCATGGCCGCGCGTGATAAGGACCCGAGCGGCGGGGTTGTTGTGTTTGAGGGCGCTGGGAGTGGCGGCGGCGGCGGTGGAGGTGGTGGAGGGGGAGGAGTCTATTGGCAGCATCCGCCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCACATCAGGGACACGTGTACGGCGGTGGCGGGTTTCATCAGATGGGGGTTGGTGGGGTTGGGATTGGAAAGGGTGGGCCCGGGTCCAACATGGGTAGGCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

206

Amino Acids

21.81

Weight (kDa)

6.52

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 26 - 95 6.1e-06 Core histone H2A/H2B/H3/H4 domain
CBFD_NFYB_HMF PF00808 31 - 95 2.3e-27 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015583)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g37320
malus_domestica MD03G1081800.v1.1
prunus_persica Prupe.6G008800_v2.0.a1
pyrus_communis pycom03g06520
rosa_chinensis RchiOBHm_Chr5g0067101
rosa_laevigata RLG00000035910 RLG00000035913
rosa_multiflora Rmu_sc0000151.1_g000011
rosa_roxburghii Rroxscaffold_1G00013970
rosa_rugosa Rorug05G0381500 Rorug05G0381800
rosa_samantha Rh5AG441800 Rh5BG458800 Rh5CG481000 Rh5DG473700
rosa_wichuraiana Rw5G041250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 319, 396
AccI GTMKAC 1 cut(s) 53
AccII CGCG 3 cut(s) 240, 377, 379
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 2 cut(s) 138, 372
AcvI CACGTG 1 cut(s) 529
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 3 cut(s) 308, 329, 533
AfiI CCNNNNNNNGG 2 cut(s) 398, 521
AflIII ACRYGT 2 cut(s) 526, 528
AluBI AGCT 2 cut(s) 67, 204
AluI AGCT 2 cut(s) 67, 204
Alw26I GTCTC 2 cut(s) 164, 193
AlwI GGATC 1 cut(s) 125
Ama87I CYCGRG 5 cut(s) 22, 43, 75, 391, 593
AoxI GGCC 6 cut(s) 138, 219, 279, 372, 590, 611
ApaI GGGCCC 1 cut(s) 594
AspLEI GCGC 4 cut(s) 133, 240, 379, 422
AspS9I GGNCC 7 cut(s) 85, 279, 388, 590, 591, 597, 612
AsuC2I CCSGG 4 cut(s) 44, 45, 594, 595
AsuHPI GGTGA 2 cut(s) 63, 202
AvaI CYCGRG 5 cut(s) 22, 43, 75, 391, 593
AvaII GGWCC 3 cut(s) 85, 388, 597
BaeGI GKGCMC 2 cut(s) 36, 594
BanII GRGCYC 1 cut(s) 594
BbrPI CACGTG 1 cut(s) 529
BbsI GAAGAC 1 cut(s) 257
BccI CCATC 3 cut(s) 263, 343, 550
BceAI ACGGC 2 cut(s) 277, 550
BcnI CCSGG 4 cut(s) 44, 45, 594, 595
BcoDI GTCTC 2 cut(s) 164, 193
BfoI RGCGCY 2 cut(s) 134, 423
BglII AGATCT 1 cut(s) 150
Bme1390I CCNGG 4 cut(s) 44, 45, 594, 595
Bme18I GGWCC 3 cut(s) 85, 388, 597
BmeT110I CYCGRG 5 cut(s) 22, 43, 75, 391, 593
BmgT120I GGNCC 7 cut(s) 85, 279, 388, 590, 591, 597, 612
BmiI GGNNCC 3 cut(s) 390, 592, 598
BmrFI CCNGG 4 cut(s) 44, 45, 594, 595
BmsI GCATC 1 cut(s) 487
BoxI GACNNNNGTC 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 257
BplI GAGNNNNNCTC 2 cut(s) 173, 205
BpuEI CTTGAG 1 cut(s) 305
BpuMI CCSGG 4 cut(s) 44, 45, 594, 595
BsaAI YACGTR 1 cut(s) 529
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 6 cut(s) 43, 74, 156, 165, 292, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 521
Bse118I RCCGGY 3 cut(s) 87, 136, 212
BseDI CCNNGG 6 cut(s) 43, 74, 156, 165, 292, 593
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 2 cut(s) 398, 521
BseRI GAGGAG 2 cut(s) 317, 477
BseSI GKGCMC 2 cut(s) 36, 594
BseYI CCCAGC 1 cut(s) 422
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 3 cut(s) 240, 377, 379
BshFI GGCC 6 cut(s) 140, 221, 281, 374, 592, 613
BsiHKCI CYCGRG 5 cut(s) 22, 43, 75, 391, 593
BsiSI CCGG 5 cut(s) 44, 88, 137, 213, 594
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 2 cut(s) 398, 521
BsmAI GTCTC 2 cut(s) 164, 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 537
BsnI GGCC 6 cut(s) 140, 221, 281, 374, 592, 613
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 5 cut(s) 22, 43, 75, 391, 593
Bsp120I GGGCCC 1 cut(s) 590
Bsp1286I GDGCHC 2 cut(s) 36, 594
Bsp143I GATC 2 cut(s) 117, 150
BspANI GGCC 6 cut(s) 140, 221, 281, 374, 592, 613
BspFNI CGCG 3 cut(s) 240, 377, 379
BspHI TCATGA 1 cut(s) 120
BspLI GGNNCC 3 cut(s) 390, 592, 598
BspPI GGATC 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 53
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 2 cut(s) 319, 396
BsrFI RCCGGY 3 cut(s) 87, 136, 212
BssAI RCCGGY 3 cut(s) 87, 136, 212
BssECI CCNNGG 6 cut(s) 43, 74, 156, 165, 292, 593
BssMI GATC 2 cut(s) 117, 150
BssNAI GTATAC 1 cut(s) 54
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 2 cut(s) 156, 165
Bst1107I GTATAC 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 2 cut(s) 53, 356
BstACI GRCGYC 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 32, 65, 138
BstF5I GGATG 1 cut(s) 478
BstFNI CGCG 3 cut(s) 240, 377, 379
BstH2I RGCGCY 2 cut(s) 134, 423
BstHHI GCGC 4 cut(s) 133, 240, 379, 422
BstKTI GATC 2 cut(s) 120, 153
BstMAI GTCTC 2 cut(s) 164, 193
BstMBI GATC 2 cut(s) 117, 150
BstPAI GACNNNNGTC 1 cut(s) 231
BstSCI CCNGG 4 cut(s) 42, 43, 592, 593
BstSLI GKGCMC 2 cut(s) 36, 594
BstUI CGCG 3 cut(s) 240, 377, 379
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BstZ17I GTATAC 1 cut(s) 54
BsuRI GGCC 6 cut(s) 140, 221, 281, 374, 592, 613
BtsCI GGATG 1 cut(s) 478
Cac8I GCNNGC 3 cut(s) 32, 65, 138
CciI TCATGA 1 cut(s) 120
CfoI GCGC 4 cut(s) 133, 240, 379, 422
Cfr10I RCCGGY 3 cut(s) 87, 136, 212
Cfr13I GGNCC 7 cut(s) 85, 279, 388, 590, 591, 597, 612
Cfr9I CCCGGG 2 cut(s) 43, 593
CseI GACGC 2 cut(s) 170, 175
Csp6I GTAC 3 cut(s) 307, 328, 532
CviAII CATG 4 cut(s) 121, 283, 370, 604
CviQI GTAC 3 cut(s) 307, 328, 532
DpnI GATC 2 cut(s) 119, 152
DpnII GATC 2 cut(s) 117, 150
EaeI YGGCCR 2 cut(s) 138, 372
Eam1104I CTCTTC 2 cut(s) 53, 356
EarI CTCTTC 2 cut(s) 53, 356
EciI GGCGGA 2 cut(s) 20, 471
Eco130I CCWWGG 2 cut(s) 156, 165
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 594
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 3 cut(s) 85, 388, 597
Eco72I CACGTG 1 cut(s) 529
Eco88I CYCGRG 5 cut(s) 22, 43, 75, 391, 593
EcoO109I RGGNCCY 1 cut(s) 388
EcoT14I CCWWGG 2 cut(s) 156, 165
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 2 cut(s) 156, 165
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 124, 286, 373, 607
FaiI YATR 6 cut(s) 54, 122, 200, 284, 371, 605
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 537
FatI CATG 4 cut(s) 120, 282, 369, 603
FauI CCCGC 3 cut(s) 23, 392, 536
FblI GTMKAC 1 cut(s) 53
FokI GGATG 1 cut(s) 465
FriOI GRGCYC 1 cut(s) 594
GlaI GCGC 4 cut(s) 132, 239, 378, 421
GsaI CCCAGC 1 cut(s) 426
HaeII RGCGCY 2 cut(s) 134, 423
HaeIII GGCC 6 cut(s) 140, 221, 281, 374, 592, 613
HapII CCGG 5 cut(s) 44, 88, 137, 213, 594
HgaI GACGC 2 cut(s) 170, 175
HhaI GCGC 4 cut(s) 133, 240, 379, 422
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 4 cut(s) 124, 286, 373, 607
Hin6I GCGC 4 cut(s) 131, 238, 377, 420
HinP1I GCGC 4 cut(s) 131, 238, 377, 420
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 3 cut(s) 8, 20, 465
HpaII CCGG 5 cut(s) 44, 88, 137, 213, 594
HphI GGTGA 2 cut(s) 63, 202
Hpy166II GTNNAC 3 cut(s) 54, 328, 532
Hpy188I TCNGA 4 cut(s) 13, 193, 226, 555
Hpy188III TCNNGA 3 cut(s) 24, 77, 121
Hpy8I GTNNAC 3 cut(s) 54, 328, 532
Hpy99I CGWCG 2 cut(s) 269, 314
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 3 cut(s) 108, 309, 528
HpyCH4V TGCA 2 cut(s) 178, 334
HpySE526I ACGT 3 cut(s) 108, 309, 528
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 4 cut(s) 124, 286, 373, 607
HspAI GCGC 4 cut(s) 131, 238, 377, 420
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 2 cut(s) 117, 150
LguI GCTCTTC 1 cut(s) 53
LweI GCATC 1 cut(s) 487
MaeII ACGT 3 cut(s) 108, 309, 528
MaeIII GTNAC 2 cut(s) 69, 93
MalI GATC 2 cut(s) 119, 152
MbiI CCGCTC 2 cut(s) 319, 396
MboI GATC 2 cut(s) 117, 150
MboII GAAGA 4 cut(s) 70, 136, 262, 373
MfeI CAATTG 1 cut(s) 99
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 2 cut(s) 36, 594
MluCI AATT 1 cut(s) 99
MlyI GAGTC 3 cut(s) 2, 14, 474
MmeI TCCRAC 1 cut(s) 249
MroNI GCCGGC 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 5 cut(s) 44, 88, 137, 213, 594
MspR9I CCNGG 4 cut(s) 44, 45, 594, 595
MunI CAATTG 1 cut(s) 99
MvnI CGCG 3 cut(s) 240, 377, 379
NaeI GCCGGC 1 cut(s) 138
NciI CCSGG 4 cut(s) 44, 45, 594, 595
NdeII GATC 2 cut(s) 117, 150
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 4 cut(s) 124, 286, 373, 607
NlaIV GGNNCC 3 cut(s) 390, 592, 598
NmeAIII GCCGAG 1 cut(s) 273
NmuCI GTSAC 1 cut(s) 69
PagI TCATGA 1 cut(s) 120
PceI AGGCCT 1 cut(s) 221
PciSI GCTCTTC 1 cut(s) 53
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdiI GCCGGC 1 cut(s) 138
PleI GAGTC 3 cut(s) 2, 14, 473
PmaCI CACGTG 1 cut(s) 529
PmlI CACGTG 1 cut(s) 529
PpsI GAGTC 3 cut(s) 2, 14, 473
Ppu21I YACGTR 1 cut(s) 529
PpuMI RGGWCCY 1 cut(s) 388
PshAI GACNNNNGTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 388
PspCI CACGTG 1 cut(s) 529
PspFI CCCAGC 1 cut(s) 422
PspN4I GGNNCC 3 cut(s) 390, 592, 598
PspOMI GGGCCC 1 cut(s) 590
PspPI GGNCC 7 cut(s) 85, 279, 388, 590, 591, 597, 612
PspPPI RGGWCCY 1 cut(s) 388
PsuI RGATCY 1 cut(s) 150
RsaI GTAC 3 cut(s) 308, 329, 533
RsaNI GTAC 3 cut(s) 307, 328, 532
SapI GCTCTTC 1 cut(s) 53
Sau3AI GATC 2 cut(s) 117, 150
Sau96I GGNCC 7 cut(s) 85, 279, 388, 590, 591, 597, 612
SchI GAGTC 3 cut(s) 2, 14, 474
ScrFI CCNGG 4 cut(s) 44, 45, 594, 595
SduI GDGCHC 2 cut(s) 36, 594
SetI ASST 8 cut(s) 69, 76, 111, 206, 312, 327, 451, 531
SfaNI GCATC 1 cut(s) 487
SgrAI CRCCGGYG 1 cut(s) 212
SinI GGWCC 3 cut(s) 85, 388, 597
SmaI CCCGGG 2 cut(s) 45, 595
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 1 cut(s) 99
SseBI AGGCCT 1 cut(s) 221
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 4 cut(s) 42, 43, 592, 593
StyI CCWWGG 2 cut(s) 156, 165
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 3 cut(s) 111, 312, 531
TaqI TCGA 2 cut(s) 300, 312
TasI AATT 1 cut(s) 99
TatI WGTACW 1 cut(s) 327
TauI GCSGC 7 cut(s) 319, 377, 399, 435, 438, 441, 487
TseFI GTSAC 1 cut(s) 69
Tsp45I GTSAC 1 cut(s) 69
TspDTI ATGAA 4 cut(s) 137, 187, 196, 539
TspMI CCCGGG 2 cut(s) 43, 593
VpaK11BI GGWCC 3 cut(s) 85, 388, 597
XmaI CCCGGG 2 cut(s) 43, 593
XmiI GTMKAC 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.