FvH4_3g44810

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013228
Physical Location & Seq
Forward (+)
37291846 .. 37293494
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44810.t1

Sequence Viewer

Length: 942 bp
ATGGGTACTTTAGTTGGGCATGTAGTACCAGGCTTTGGGTTTGTTCTGATTGGATTATGGCACCTCTTCAACCACATCAAGCTCCATCTTCAACATCCCAACTCCTACAGGGCACCAATATGGTTTCCCGCCTCCAAGTTCAGGTACTTGGAGCTCTTGTTGATCATCTTAGGTTCATCAGCCTCCGTTGCCATGGAACTCTTCATCAGTCCTGAAAAACACCAACCGTTCGACGATGACGGAACCATCCCTTCAAACCATCTCCACAACTTCGAGCACGCCTCAATCTCCATGACTTTCTTGGTCTACGCTGCCTTGGCTATCGTTCTCGACAAATTAGCACCTAGAGCTCAGCATGGTCTGACCCAAATCCTCGGAGCCGTAGCCTTTGGTCAGCAACTGCTACTCTTTCACCTTCACTCTTCTGACCACTTGGGACCTGAGGGCCAATACCACTTGCTTCTTCAGCTTGTGATTCTTGTCTCCTTGATCACAACCCTAGTGGGACTTGGTTTCCCCAAGTGCTTCCTGATCAGCTTTGTGAGGTCACTCAGTATATTCTTCCAAGGTATCTGGCTTATGGTCATGGGGGTTATGCTCTGGACACCTGGGTTGATTTCCAAAGGTTGTTTTCTAAATGATGAAGAGGGTCACGAAGTGGTTAAGTGCCAAAGCGAGGAGGCACGTCACAGAGCACTGTCGTTGGTGAATCTTCTGTTTAGTTGGTTCTTGATTGGAATTGCCGTTTTTGGGGTGGCCTTCTATTTGGTTTTGGTTAAGTTTTACGGTGAAAAGGTGGAGTACTTTACTCTGGGAAGTGACCGTGAATCTGAAGAAGATGATGATGTGGAATCTCAGAAGAGAAGCAATGTTGGAGACTCGAAGAGCTTTGTGGATCATATGGGAAAGACAGCCTTTGCGCCAATAGACATGGAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

314

Amino Acids

35.1

Weight (kDa)

5.98

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 120 - 256 3.6e-50 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55230
fragaria_vesca FvH4_3g44810
malus_domestica MD03G1010300.v1.1 MD03G1010400.v1.1 MD11G1012300.v1.1
prunus_persica Prupe.6G010200_v2.0.a1
pyrus_communis pycom03g00920 pycom11g00800
rosa_chinensis RchiOBHm_Chr5g0081481
rosa_laevigata RLG00000036942
rosa_multiflora Rmu_sc0001783.1_g000010
rosa_roxburghii Rroxscaffold_1G00002050
rosa_rugosa Rorug05G0476700
rosa_samantha Rh5AG528200 Rh5BG552800 Rh5CG575600 Rh5DG563000
rosa_wichuraiana Rw5G049130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 112
AccB7I CCANNNNNTGG 1 cut(s) 35
AccI GTMKAC 1 cut(s) 306
AciI CCGC 1 cut(s) 129
AclWI GGATC 1 cut(s) 903
AcuI CTGAAG 2 cut(s) 449, 852
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 4 cut(s) 7, 27, 146, 803
AfiI CCNNNNNNNGG 4 cut(s) 35, 141, 676, 750
AgsI TTSAA 3 cut(s) 70, 92, 255
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 2 cut(s) 28, 607
AluBI AGCT 6 cut(s) 82, 154, 350, 469, 537, 888
AluI AGCT 6 cut(s) 82, 154, 350, 469, 537, 888
Alw21I GWGCWC 4 cut(s) 156, 279, 352, 697
Alw26I GTCTC 2 cut(s) 487, 870
AlwI GGATC 1 cut(s) 903
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 2 cut(s) 445, 756
ApeKI GCWGC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 922
AspS9I GGNCC 2 cut(s) 437, 445
AsuHPI GGTGA 3 cut(s) 404, 718, 800
AvaII GGWCC 1 cut(s) 437
AxyI CCTNAGG 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 115
BaeI ACNNNNGTAYC 1 cut(s) 30
BanI GGYRCC 2 cut(s) 60, 112
BanII GRGCYC 2 cut(s) 156, 352
Bbv12I GWGCWC 4 cut(s) 156, 279, 352, 697
BbvI GCAGC 1 cut(s) 298
BccI CCATC 3 cut(s) 93, 254, 267
BceAI ACGGC 2 cut(s) 365, 728
BciT130I CCWGG 2 cut(s) 30, 609
BclI TGATCA 3 cut(s) 162, 489, 531
BcoDI GTCTC 2 cut(s) 487, 870
BfaI CTAG 2 cut(s) 345, 500
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 1 cut(s) 312
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 313
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 2 cut(s) 30, 609
Bme18I GGWCC 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 437, 445
BmiI GGNNCC 5 cut(s) 62, 114, 244, 379, 438
BmrFI CCNGG 2 cut(s) 30, 609
BplI GAGNNNNNCTC 2 cut(s) 266, 298
Bpu1102I GCTNAGC 1 cut(s) 351
BsaJI CCNNGG 5 cut(s) 192, 315, 373, 565, 608
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 141, 676, 750
Bse21I CCTNAGG 1 cut(s) 441
Bse3DI GCAATG 1 cut(s) 874
BseBI CCWGG 2 cut(s) 30, 609
BseDI CCNNGG 5 cut(s) 192, 315, 373, 565, 608
BseGI GGATG 2 cut(s) 94, 246
BseLI CCNNNNNNNGG 4 cut(s) 35, 141, 676, 750
BseMI GCAATG 1 cut(s) 874
BseMII CTCAG 4 cut(s) 365, 432, 565, 869
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 1 cut(s) 298
BshFI GGCC 2 cut(s) 447, 758
BshNI GGYRCC 2 cut(s) 60, 112
BsiHKAI GWGCWC 4 cut(s) 156, 279, 352, 697
BslFI GGGAC 2 cut(s) 450, 519
BslI CCNNNNNNNGG 4 cut(s) 35, 141, 676, 750
BsmAI GTCTC 2 cut(s) 487, 870
BsmFI GGGAC 2 cut(s) 450, 519
BsnI GGCC 2 cut(s) 447, 758
Bsp1286I GDGCHC 5 cut(s) 115, 156, 279, 352, 697
Bsp143I GATC 4 cut(s) 162, 489, 531, 895
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 1 cut(s) 129
BspANI GGCC 2 cut(s) 447, 758
BspCNI CTCAG 4 cut(s) 364, 433, 564, 868
BspLI GGNNCC 5 cut(s) 62, 114, 244, 379, 438
BspPI GGATC 1 cut(s) 903
BspQI GCTCTTC 1 cut(s) 878
BspT107I GGYRCC 2 cut(s) 60, 112
BsrDI GCAATG 1 cut(s) 874
BssECI CCNNGG 5 cut(s) 192, 315, 373, 565, 608
BssMI GATC 4 cut(s) 162, 489, 531, 895
BssT1I CCWWGG 3 cut(s) 192, 315, 565
Bst2UI CCWGG 2 cut(s) 30, 609
Bst4CI ACNGT 4 cut(s) 228, 699, 788, 824
Bst6I CTCTTC 6 cut(s) 71, 206, 427, 639, 854, 878
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 5 cut(s) 169, 351, 441, 551, 855
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 94, 246
BstHHI GCGC 1 cut(s) 922
BstKTI GATC 4 cut(s) 165, 492, 534, 898
BstMAI GTCTC 2 cut(s) 487, 870
BstMBI GATC 4 cut(s) 162, 489, 531, 895
BstMWI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
BstNI CCWGG 2 cut(s) 30, 609
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 28, 607
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 1 cut(s) 298
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 2 cut(s) 447, 758
BtgI CCRYGG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 2 cut(s) 94, 246
BtsIMutI CAGTG 1 cut(s) 695
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 1 cut(s) 922
Cfr13I GGNCC 2 cut(s) 437, 445
Csp6I GTAC 4 cut(s) 6, 26, 145, 802
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviAII CATG 6 cut(s) 20, 193, 292, 356, 586, 931
CviQI GTAC 4 cut(s) 6, 26, 145, 802
DdeI CTNAG 5 cut(s) 169, 351, 441, 551, 855
DpnI GATC 4 cut(s) 164, 491, 533, 897
DpnII GATC 4 cut(s) 162, 489, 531, 895
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 6 cut(s) 71, 206, 427, 639, 854, 878
EarI CTCTTC 6 cut(s) 71, 206, 427, 639, 854, 878
Ecl136II GAGCTC 2 cut(s) 154, 350
Eco130I CCWWGG 3 cut(s) 192, 315, 565
Eco24I GRGCYC 2 cut(s) 156, 352
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 2 cut(s) 154, 350
Eco57I CTGAAG 2 cut(s) 449, 852
Eco81I CCTNAGG 1 cut(s) 441
EcoICRI GAGCTC 2 cut(s) 154, 350
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 28, 607
EcoT14I CCWWGG 3 cut(s) 192, 315, 565
EcoT38I GRGCYC 2 cut(s) 156, 352
ErhI CCWWGG 3 cut(s) 192, 315, 565
FaeI CATG 6 cut(s) 23, 196, 295, 359, 589, 934
FalI AAGNNNNNCTT 2 cut(s) 899, 931
FaqI GGGAC 2 cut(s) 450, 519
FatI CATG 6 cut(s) 19, 192, 291, 355, 585, 930
FauI CCCGC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 3 cut(s) 162, 489, 531
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 2 cut(s) 81, 233
FriOI GRGCYC 2 cut(s) 156, 352
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 345, 500
GlaI GCGC 1 cut(s) 921
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 2 cut(s) 447, 758
HhaI GCGC 1 cut(s) 922
Hin1II CATG 6 cut(s) 23, 196, 295, 359, 589, 934
Hin6I GCGC 1 cut(s) 920
HinP1I GCGC 1 cut(s) 920
HinfI GANTC 5 cut(s) 475, 709, 827, 851, 878
HphI GGTGA 3 cut(s) 404, 718, 800
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 6 cut(s) 48, 363, 377, 427, 832, 858
Hpy188III TCNNGA 6 cut(s) 212, 329, 529, 601, 653, 730
Hpy8I GTNNAC 1 cut(s) 307
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 3 cut(s) 261, 425, 769
HpyCH4III ACNGT 4 cut(s) 228, 699, 788, 824
HpyCH4IV ACGT 1 cut(s) 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
HpyF3I CTNAG 5 cut(s) 169, 351, 441, 551, 855
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 6 cut(s) 23, 196, 295, 359, 589, 934
HspAI GCGC 1 cut(s) 920
Ksp22I TGATCA 3 cut(s) 162, 489, 531
Kzo9I GATC 4 cut(s) 162, 489, 531, 895
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 3 cut(s) 87, 151, 377
Lsp1109I GCAGC 1 cut(s) 298
MaeI CTAG 2 cut(s) 345, 500
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 4 cut(s) 546, 650, 686, 818
MalI GATC 4 cut(s) 164, 491, 533, 897
MboI GATC 4 cut(s) 162, 489, 531, 895
MhlI GDGCHC 5 cut(s) 115, 156, 279, 352, 697
MluCI AATT 2 cut(s) 335, 738
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 853
MseI TTAA 2 cut(s) 663, 777
MslI CAYNNNNRTG 1 cut(s) 118
MspR9I CCNGG 2 cut(s) 30, 609
MvaI CCWGG 2 cut(s) 30, 609
MwoI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
NcoI CCATGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 900
NdeII GATC 4 cut(s) 162, 489, 531, 895
NlaIII CATG 6 cut(s) 23, 196, 295, 359, 589, 934
NlaIV GGNNCC 5 cut(s) 62, 114, 244, 379, 438
NmuCI GTSAC 4 cut(s) 546, 650, 686, 818
NspI RCATGY 1 cut(s) 23
PciSI GCTCTTC 1 cut(s) 878
PfeI GAWTC 4 cut(s) 475, 709, 827, 851
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
PpuMI RGGWCCY 1 cut(s) 437
Psp124BI GAGCTC 2 cut(s) 156, 352
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 28, 607
PspGI CCWGG 2 cut(s) 28, 607
PspN4I GGNNCC 5 cut(s) 62, 114, 244, 379, 438
PspPI GGNCC 2 cut(s) 437, 445
PspPPI RGGWCCY 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 400
RsaI GTAC 4 cut(s) 7, 27, 146, 803
RsaNI GTAC 4 cut(s) 6, 26, 145, 802
RseI CAYNNNNRTG 1 cut(s) 118
SacI GAGCTC 2 cut(s) 156, 352
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 2 cut(s) 663, 777
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 4 cut(s) 162, 489, 531, 895
Sau96I GGNCC 2 cut(s) 437, 445
ScaI AGTACT 1 cut(s) 803
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 2 cut(s) 30, 609
SduI GDGCHC 5 cut(s) 115, 156, 279, 352, 697
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 118
Sse9I AATT 2 cut(s) 335, 738
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 345, 500
SstI GAGCTC 2 cut(s) 156, 352
StyD4I CCNGG 2 cut(s) 28, 607
StyI CCWWGG 3 cut(s) 192, 315, 565
TaaI ACNGT 4 cut(s) 228, 699, 788, 824
TaiI ACGT 1 cut(s) 688
TaqI TCGA 4 cut(s) 231, 273, 330, 881
TasI AATT 2 cut(s) 335, 738
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 4 cut(s) 475, 709, 827, 851
Tru1I TTAA 2 cut(s) 663, 777
Tru9I TTAA 2 cut(s) 663, 777
TscAI CASTG 1 cut(s) 702
TseFI GTSAC 4 cut(s) 546, 650, 686, 818
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 4 cut(s) 546, 650, 686, 818
TspDTI ATGAA 3 cut(s) 165, 193, 657
TspGWI ACGGA 2 cut(s) 175, 255
TspRI CASTG 1 cut(s) 702
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 437
XceI RCATGY 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 306
XspI CTAG 2 cut(s) 345, 500
ZrmI AGTACT 1 cut(s) 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.