Rroxscaffold_1G00002050

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013228
Physical Location & Seq
Reverse (-)
2461519 .. 2463029
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002050.1

Sequence Viewer

Length: 942 bp
ATGGGTACTTTAATTGGGCATGTAGTACCAGGCTTTGGTTTTGTTCTAATTGGATTATGGCACCTCTTCAACCACATCAAGCTCCACCTTCAACATCCCAACTCCTACACAGCACCAACATGGTTTCCCGCCTCCAAGTTCAAGTACTTGGAGCTCTTGTTGATCATCCTAGGTTCATCAGCCTCCATTGCCATGGAACTCTTCATCAGCCCCGAAAAACACCATCCATTAGACGATGACGGAACCATCCCTTCATACCATCTCCACAACTTCGAGCACGCCACAATCTCCATGAATTTGTTGGTCTACGCTGCCTTTGCCATCGTTCTCGATAAATTAGCCCCTAGAGCTCAGCATGGTCTGACCCAACTCCTCGGAGCCATAGCCTTTGGTCAGGAACTGCTTCTCTTTCACCTTCACTCTTCTGACCACATGGGACCTGAGGGCCGATTCCACTTGCTTCTTCAGCTTGTGATTCTTGTCTCCTTTACCACAACCCTAATGGGAATTGGTTTCCCCAAGAGCTTCTTGGTCAGCTTTGTGAGGTCACTCAGCATATTCTTCCAAGGTCTCTGGCTTATTGTCATGGGGCTCATGCTTTGGACACCTGGATTGATTTCCAAAGGTTGTTTCCTAAATGATGAAGATGGTCACGAAGTGGTTAGGTGCCAAAGCGAGGAGGCACGTCAAAGAGCCCTGTCCTTGGTGAATCTTCAGTTCAGTTGGTTCTTGATTGTAGTTGCCGTTTTTGGGGTGGCTTTCTATTTGGTTTTGATTATGTTCTACGGTGAAAAGGTGGAGTACTTTACTCTGGGAAGTGACCGTGAATATGAAGAAGATGATGATGTGGAATCTCAGAAGAAAAGCAATGTTGGAGACTCGAAGAGTTTTGTGGATCATATGGGAAAATCAGCCTTTGCGCCAATAGACATAGAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

313

Amino Acids

35.23

Weight (kDa)

5.81

Isoelectric Point (pI)

33.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 120 - 256 7.2e-52 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55230
fragaria_vesca FvH4_3g44810
malus_domestica MD03G1010300.v1.1 MD03G1010400.v1.1 MD11G1012300.v1.1
prunus_persica Prupe.6G010200_v2.0.a1
pyrus_communis pycom03g00920 pycom11g00800
rosa_chinensis RchiOBHm_Chr5g0081481
rosa_laevigata RLG00000036942
rosa_multiflora Rmu_sc0001783.1_g000010
rosa_roxburghii Rroxscaffold_1G00002050
rosa_rugosa Rorug05G0476700
rosa_samantha Rh5AG528200 Rh5BG552800 Rh5CG575600 Rh5DG563000
rosa_wichuraiana Rw5G049130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 666
AccB7I CCANNNNNTGG 1 cut(s) 35
AccI GTMKAC 1 cut(s) 306
AciI CCGC 1 cut(s) 129
AclWI GGATC 1 cut(s) 903
AcsI RAATTY 1 cut(s) 295
AcuI CTGAAG 2 cut(s) 449, 698
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 4 cut(s) 7, 27, 146, 803
AfiI CCNNNNNNNGG 4 cut(s) 35, 676, 703, 750
AgsI TTSAA 3 cut(s) 70, 92, 142
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 2 cut(s) 28, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 6 cut(s) 82, 154, 350, 469, 525, 537
AluI AGCT 6 cut(s) 82, 154, 350, 469, 525, 537
Alw21I GWGCWC 3 cut(s) 156, 279, 352
Alw26I GTCTC 3 cut(s) 487, 575, 870
AlwI GGATC 1 cut(s) 903
AlwNI CAGNNNCTG 1 cut(s) 400
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 1 cut(s) 295
Asp700I GAANNNNTTC 1 cut(s) 402
AspA2I CCTAGG 1 cut(s) 169
AspLEI GCGC 1 cut(s) 922
AspS9I GGNCC 2 cut(s) 437, 445
AsuHPI GGTGA 3 cut(s) 404, 718, 800
AvaII GGWCC 1 cut(s) 437
AvrII CCTAGG 1 cut(s) 169
AxyI CCTNAGG 1 cut(s) 441
BanI GGYRCC 2 cut(s) 60, 666
BanII GRGCYC 4 cut(s) 156, 352, 594, 697
Bbv12I GWGCWC 3 cut(s) 156, 279, 352
BbvI GCAGC 1 cut(s) 298
BccI CCATC 5 cut(s) 231, 254, 267, 329, 641
BceAI ACGGC 1 cut(s) 728
BciT130I CCWGG 2 cut(s) 30, 609
BclI TGATCA 1 cut(s) 162
BcoDI GTCTC 3 cut(s) 487, 575, 870
BfaI CTAG 2 cut(s) 170, 345
BisI GCNGC 1 cut(s) 312
BlnI CCTAGG 1 cut(s) 169
BlpI GCTNAGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 313
BmcAI AGTACT 2 cut(s) 146, 803
Bme1390I CCNGG 2 cut(s) 30, 609
Bme18I GGWCC 1 cut(s) 437
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 2 cut(s) 437, 445
BmiI GGNNCC 5 cut(s) 62, 244, 379, 438, 668
BmrFI CCNGG 2 cut(s) 30, 609
Bpu1102I GCTNAGC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 575
BsaJI CCNNGG 5 cut(s) 169, 192, 373, 565, 702
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 4 cut(s) 35, 676, 703, 750
Bse21I CCTNAGG 1 cut(s) 441
Bse3DI GCAATG 2 cut(s) 186, 874
BseBI CCWGG 2 cut(s) 30, 609
BseDI CCNNGG 5 cut(s) 169, 192, 373, 565, 702
BseGI GGATG 4 cut(s) 94, 165, 223, 246
BseLI CCNNNNNNNGG 4 cut(s) 35, 676, 703, 750
BseMI GCAATG 2 cut(s) 186, 874
BseMII CTCAG 4 cut(s) 365, 432, 565, 869
BseRI GAGGAG 2 cut(s) 362, 692
BseXI GCAGC 1 cut(s) 298
BshFI GGCC 1 cut(s) 447
BshNI GGYRCC 2 cut(s) 60, 666
BsiHKAI GWGCWC 3 cut(s) 156, 279, 352
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 4 cut(s) 35, 676, 703, 750
BsmAI GTCTC 3 cut(s) 487, 575, 870
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 1 cut(s) 447
Bso31I GGTCTC 1 cut(s) 575
Bsp1286I GDGCHC 5 cut(s) 156, 279, 352, 594, 697
Bsp143I GATC 2 cut(s) 162, 895
Bsp1720I GCTNAGC 1 cut(s) 351
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 1 cut(s) 129
BspANI GGCC 1 cut(s) 447
BspCNI CTCAG 4 cut(s) 364, 433, 564, 868
BspLI GGNNCC 5 cut(s) 62, 244, 379, 438, 668
BspPI GGATC 1 cut(s) 903
BspT107I GGYRCC 2 cut(s) 60, 666
BspTNI GGTCTC 1 cut(s) 575
BsrDI GCAATG 2 cut(s) 186, 874
BssECI CCNNGG 5 cut(s) 169, 192, 373, 565, 702
BssMI GATC 2 cut(s) 162, 895
BssT1I CCWWGG 4 cut(s) 169, 192, 565, 702
Bst2UI CCWGG 2 cut(s) 30, 609
Bst4CI ACNGT 2 cut(s) 788, 824
Bst6I CTCTTC 4 cut(s) 71, 206, 427, 878
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 4 cut(s) 351, 441, 551, 855
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 4 cut(s) 94, 165, 223, 246
BstHHI GCGC 1 cut(s) 922
BstKTI GATC 2 cut(s) 165, 898
BstMAI GTCTC 3 cut(s) 487, 575, 870
BstMBI GATC 2 cut(s) 162, 895
BstMWI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
BstNI CCWGG 2 cut(s) 30, 609
BstNSI RCATGY 1 cut(s) 23
BstSCI CCNGG 2 cut(s) 28, 607
BstV1I GCAGC 1 cut(s) 298
BstXI CCANNNNNNTGG 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 441
BsuRI GGCC 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 4 cut(s) 94, 165, 223, 246
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 400
CfoI GCGC 1 cut(s) 922
Cfr13I GGNCC 2 cut(s) 437, 445
Csp6I GTAC 4 cut(s) 6, 26, 145, 802
CviAII CATG 8 cut(s) 20, 120, 193, 292, 356, 433, 586, 595
CviQI GTAC 4 cut(s) 6, 26, 145, 802
DdeI CTNAG 4 cut(s) 351, 441, 551, 855
DpnI GATC 2 cut(s) 164, 897
DpnII GATC 2 cut(s) 162, 895
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 4 cut(s) 71, 206, 427, 878
EarI CTCTTC 4 cut(s) 71, 206, 427, 878
Ecl136II GAGCTC 2 cut(s) 154, 350
Eco130I CCWWGG 4 cut(s) 169, 192, 565, 702
Eco24I GRGCYC 4 cut(s) 156, 352, 594, 697
Eco31I GGTCTC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 437
Eco53kI GAGCTC 2 cut(s) 154, 350
Eco57I CTGAAG 2 cut(s) 449, 698
Eco81I CCTNAGG 1 cut(s) 441
EcoICRI GAGCTC 2 cut(s) 154, 350
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 28, 607
EcoT14I CCWWGG 4 cut(s) 169, 192, 565, 702
EcoT38I GRGCYC 4 cut(s) 156, 352, 594, 697
ErhI CCWWGG 4 cut(s) 169, 192, 565, 702
FaeI CATG 8 cut(s) 23, 123, 196, 295, 359, 436, 589, 598
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 450
FatI CATG 8 cut(s) 19, 119, 192, 291, 355, 432, 585, 594
FauI CCCGC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 900
FbaI TGATCA 1 cut(s) 162
FblI GTMKAC 1 cut(s) 306
Fnu4HI GCNGC 1 cut(s) 312
FokI GGATG 4 cut(s) 81, 152, 210, 233
FriOI GRGCYC 4 cut(s) 156, 352, 594, 697
Fsp4HI GCNGC 1 cut(s) 312
FspBI CTAG 2 cut(s) 170, 345
GlaI GCGC 1 cut(s) 921
GluI GCNGC 1 cut(s) 312
HaeIII GGCC 1 cut(s) 447
HhaI GCGC 1 cut(s) 922
Hin1II CATG 8 cut(s) 23, 123, 196, 295, 359, 436, 589, 598
Hin6I GCGC 1 cut(s) 920
HinP1I GCGC 1 cut(s) 920
HinfI GANTC 5 cut(s) 450, 475, 709, 851, 878
HphI GGTGA 3 cut(s) 404, 718, 800
Hpy166II GTNNAC 1 cut(s) 307
Hpy188I TCNGA 4 cut(s) 363, 377, 427, 858
Hpy188III TCNNGA 4 cut(s) 329, 395, 653, 730
Hpy8I GTNNAC 1 cut(s) 307
HpyAV CCTTC 3 cut(s) 98, 261, 425
HpyCH4III ACNGT 2 cut(s) 788, 824
HpyCH4IV ACGT 1 cut(s) 685
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
HpyF3I CTNAG 4 cut(s) 351, 441, 551, 855
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 8 cut(s) 23, 123, 196, 295, 359, 436, 589, 598
HspAI GCGC 1 cut(s) 920
Ksp22I TGATCA 1 cut(s) 162
Kzo9I GATC 2 cut(s) 162, 895
LmnI GCTCC 3 cut(s) 87, 151, 377
LpnPI CCDG 9 cut(s) 15, 42, 380, 453, 559, 594, 621, 710, 797
Lsp1109I GCAGC 1 cut(s) 298
MaeI CTAG 2 cut(s) 170, 345
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 3 cut(s) 546, 650, 818
MalI GATC 2 cut(s) 164, 897
MboI GATC 2 cut(s) 162, 895
MhlI GDGCHC 5 cut(s) 156, 279, 352, 594, 697
MluCI AATT 5 cut(s) 12, 48, 295, 335, 507
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 853
MnlI CCTC 8 cut(s) 74, 142, 193, 383, 436, 537, 670, 673
MroXI GAANNNNTTC 1 cut(s) 402
MseI TTAA 1 cut(s) 11
MslI CAYNNNNRTG 2 cut(s) 118, 191
MspR9I CCNGG 2 cut(s) 30, 609
MvaI CCWGG 2 cut(s) 30, 609
MwoI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
NcoI CCATGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 900
NdeII GATC 2 cut(s) 162, 895
NlaIII CATG 8 cut(s) 23, 123, 196, 295, 359, 436, 589, 598
NlaIV GGNNCC 5 cut(s) 62, 244, 379, 438, 668
NmuCI GTSAC 3 cut(s) 546, 650, 818
NspI RCATGY 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 4 cut(s) 450, 475, 709, 851
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 1 cut(s) 313
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
PpuMI RGGWCCY 1 cut(s) 437
Psp124BI GAGCTC 2 cut(s) 156, 352
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 28, 607
PspGI CCWGG 2 cut(s) 28, 607
PspN4I GGNNCC 5 cut(s) 62, 244, 379, 438, 668
PspPI GGNCC 2 cut(s) 437, 445
PspPPI RGGWCCY 1 cut(s) 437
PstNI CAGNNNCTG 1 cut(s) 400
RsaI GTAC 4 cut(s) 7, 27, 146, 803
RsaNI GTAC 4 cut(s) 6, 26, 145, 802
RseI CAYNNNNRTG 2 cut(s) 118, 191
SacI GAGCTC 2 cut(s) 156, 352
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 1 cut(s) 312
Sau3AI GATC 2 cut(s) 162, 895
Sau96I GGNCC 2 cut(s) 437, 445
ScaI AGTACT 2 cut(s) 146, 803
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 2 cut(s) 30, 609
SduI GDGCHC 5 cut(s) 156, 279, 352, 594, 697
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 118, 191
Sse9I AATT 5 cut(s) 12, 48, 295, 335, 507
SsiI CCGC 1 cut(s) 129
SspMI CTAG 2 cut(s) 170, 345
SstI GAGCTC 2 cut(s) 156, 352
StyD4I CCNGG 2 cut(s) 28, 607
StyI CCWWGG 4 cut(s) 169, 192, 565, 702
TaaI ACNGT 2 cut(s) 788, 824
TaiI ACGT 1 cut(s) 688
TaqI TCGA 3 cut(s) 273, 330, 881
TasI AATT 5 cut(s) 12, 48, 295, 335, 507
TatI WGTACW 2 cut(s) 144, 801
TfiI GAWTC 4 cut(s) 450, 475, 709, 851
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseFI GTSAC 3 cut(s) 546, 650, 818
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 3 cut(s) 546, 650, 818
TspDTI ATGAA 6 cut(s) 165, 193, 243, 308, 657, 846
TspGWI ACGGA 1 cut(s) 255
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 1 cut(s) 295
XceI RCATGY 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 3 cut(s) 298, 499, 526
XmaJI CCTAGG 1 cut(s) 169
XmiI GTMKAC 1 cut(s) 306
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 170, 345
ZrmI AGTACT 2 cut(s) 146, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.