MD03G1010400.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013228
Physical Location & Seq
Reverse (-)
802304 .. 803254
951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010400.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGCACTTTAGTTGGGCATGTAGTACCAGGTTTTGGGTTTCTTCTGATTGGCCTATGGCACCTCTTTAATCACATCAAACTCCATCTTAACCAACCAAACTCCTACATAGCTCCAGCTTGGTTTCCCACCTCCAAATTCAAGTATTTGGAGCTCTTCTTAATCATGGGAGGCTCATCTGCCTCCATTGCCATGGAACTCTTCATAGGCCCAGAGAAACACCAACCGTTCGATGACGATGGAACCATCCCCACCAACCATCTCCACAACTTTGAGCACTCTTCAATATCCATGGCTTTCTTGGTCTATGCAGCTTTTGCCATCGTCCTCGACAAAATTGCCCCAAAAGCACAACATGGTTTAACCCAACTCCTTGGAGCCATAGCCTTTGGGCAGCAGCTTCTTCTCTTTCACCTTCACTCTGCTGATCACATGGGACCTGAAGGCCAATACCACATGCTTCTGCAGCTTGTGGTCCTTGTTTCTTTGACCACAGCCCTTATGGGAATTGGTTTTCCTAAGAGTTTTCTGGTCAGCTTTGTGAGGTCACTTAGCATATTTTTCCAAGGGGTTTGGCTTATGGTCATGGGGTTCATGCTCTGGACACCTGACTTGATGCCAAAAGGTTGTTTCATGAACTTAGAGGAGGGTCACAAAGTGGTTAGGTGCCATGGAGATGAGGCACGTCACAGAGCCAAGTCCTTGGTGAATCTTCAATTTAGTTGGTTCTTGGTGGGAATAGCCATTTTTGGGGTGTCCTTCTATTTGGTTTTGGTTAAGGTTTACGGCGAAAAGGTTGAGTATTTTTCATTGACAAGGCTACGCGAAGAAGATGAAGAGGATACGGATCTTGATGTTGAATCTCAAAAGATAAGTAAGCTTGGAGACTCAAAGAGTTTTGTTAATCATATGGGAAAAACAGCCTTTGCGCCCATAGACATGGAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

317

Amino Acids

35.55

Weight (kDa)

6.26

Isoelectric Point (pI)

30.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 120 - 256 4.2e-55 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55230
fragaria_vesca FvH4_3g44810
malus_domestica MD03G1010300.v1.1 MD03G1010400.v1.1 MD11G1012300.v1.1
prunus_persica Prupe.6G010200_v2.0.a1
pyrus_communis pycom03g00920 pycom11g00800
rosa_chinensis RchiOBHm_Chr5g0081481
rosa_laevigata RLG00000036942
rosa_multiflora Rmu_sc0001783.1_g000010
rosa_roxburghii Rroxscaffold_1G00002050
rosa_rugosa Rorug05G0476700
rosa_samantha Rh5AG528200 Rh5BG552800 Rh5CG575600 Rh5DG563000
rosa_wichuraiana Rw5G049130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 60, 666
AccB7I CCANNNNNTGG 1 cut(s) 35
AccII CGCG 1 cut(s) 825
AclWI GGATC 1 cut(s) 855
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 658
AfaI GTAC 1 cut(s) 27
AfiI CCNNNNNNNGG 2 cut(s) 35, 750
AgsI TTSAA 4 cut(s) 142, 285, 716, 860
AjiI CACGTC 1 cut(s) 686
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 8 cut(s) 113, 119, 154, 314, 400, 469, 537, 880
AluI AGCT 8 cut(s) 113, 119, 154, 314, 400, 469, 537, 880
Alw21I GWGCWC 2 cut(s) 156, 279
Alw26I GTCTC 1 cut(s) 879
AlwI GGATC 1 cut(s) 855
AoxI GGCC 3 cut(s) 52, 208, 445
ApeKI GCWGC 4 cut(s) 311, 394, 397, 466
ApoI RAATTY 1 cut(s) 137
AspLEI GCGC 1 cut(s) 931
AspS9I GGNCC 3 cut(s) 209, 437, 475
AsuHPI GGTGA 2 cut(s) 404, 718
AvaII GGWCC 2 cut(s) 437, 475
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 60, 666
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 2 cut(s) 156, 279
BbvI GCAGC 4 cut(s) 323, 406, 409, 478
BccI CCATC 5 cut(s) 93, 233, 254, 267, 329
BceAI ACGGC 1 cut(s) 802
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 1 cut(s) 30
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 1 cut(s) 427
BcoDI GTCTC 1 cut(s) 879
BfmI CTRYAG 1 cut(s) 464
BfuI GTATCC 1 cut(s) 835
BisI GCNGC 4 cut(s) 312, 395, 398, 467
BlsI GCNGC 4 cut(s) 313, 396, 399, 468
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 2 cut(s) 437, 475
BmgBI CACGTC 1 cut(s) 686
BmgT120I GGNCC 3 cut(s) 209, 437, 475
BmiI GGNNCC 5 cut(s) 62, 244, 379, 438, 668
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 1 cut(s) 606
BpmI CTGGAG 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 846
BsaJI CCNNGG 6 cut(s) 192, 291, 373, 565, 670, 702
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 750
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 6 cut(s) 192, 291, 373, 565, 670, 702
BseGI GGATG 1 cut(s) 246
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 2 cut(s) 35, 750
BseMI GCAATG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 4 cut(s) 323, 406, 409, 478
Bsh1236I CGCG 1 cut(s) 825
BshFI GGCC 3 cut(s) 54, 210, 447
BshNI GGYRCC 2 cut(s) 60, 666
BsiHKAI GWGCWC 2 cut(s) 156, 279
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 35, 750
BsmAI GTCTC 1 cut(s) 879
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 3 cut(s) 54, 210, 447
Bsp1286I GDGCHC 3 cut(s) 8, 156, 279
Bsp143I GATC 2 cut(s) 427, 847
Bsp19I CCATGG 3 cut(s) 192, 291, 670
BspANI GGCC 3 cut(s) 54, 210, 447
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 5 cut(s) 62, 244, 379, 438, 668
BspMAI CTGCAG 1 cut(s) 468
BspPI GGATC 1 cut(s) 855
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 2 cut(s) 60, 666
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 6 cut(s) 192, 291, 373, 565, 670, 702
BssMI GATC 2 cut(s) 427, 847
BssT1I CCWWGG 6 cut(s) 192, 291, 373, 565, 670, 702
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 4 cut(s) 161, 206, 286, 831
BstAPI GCANNNNNTGC 1 cut(s) 317
BstDEI CTNAG 3 cut(s) 519, 551, 640
BstDSI CCRYGG 3 cut(s) 192, 291, 670
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 1 cut(s) 825
BstHHI GCGC 1 cut(s) 931
BstKTI GATC 2 cut(s) 430, 850
BstMAI GTCTC 1 cut(s) 879
BstMBI GATC 2 cut(s) 427, 847
BstMWI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 2 cut(s) 23, 460
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 464
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 4 cut(s) 323, 406, 409, 478
BstX2I RGATCY 1 cut(s) 847
BstXI CCANNNNNNTGG 4 cut(s) 193, 374, 703, 940
BstYI RGATCY 1 cut(s) 847
BsuI GTATCC 1 cut(s) 835
BsuRI GGCC 3 cut(s) 54, 210, 447
BtgI CCRYGG 3 cut(s) 192, 291, 670
BtrI CACGTC 1 cut(s) 686
BtsCI GGATG 1 cut(s) 246
CciI TCATGA 1 cut(s) 633
CfoI GCGC 1 cut(s) 931
Cfr13I GGNCC 3 cut(s) 209, 437, 475
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 1 cut(s) 26
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 3 cut(s) 519, 551, 640
DpnI GATC 2 cut(s) 429, 849
DpnII GATC 2 cut(s) 427, 847
DraIII CACNNNGTG 1 cut(s) 658
Eam1104I CTCTTC 4 cut(s) 161, 206, 286, 831
EarI CTCTTC 4 cut(s) 161, 206, 286, 831
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 6 cut(s) 192, 291, 373, 565, 670, 702
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 437, 475
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 6 cut(s) 192, 291, 373, 565, 670, 702
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 6 cut(s) 192, 291, 373, 565, 670, 702
FaqI GGGAC 1 cut(s) 450
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 4 cut(s) 312, 395, 398, 467
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 312, 395, 398, 467
GlaI GCGC 1 cut(s) 930
GluI GCNGC 4 cut(s) 312, 395, 398, 467
GsuI CTGGAG 1 cut(s) 99
HaeIII GGCC 3 cut(s) 54, 210, 447
HhaI GCGC 1 cut(s) 931
Hin6I GCGC 1 cut(s) 929
HinP1I GCGC 1 cut(s) 929
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 3 cut(s) 709, 860, 887
HphI GGTGA 2 cut(s) 404, 718
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 3 cut(s) 601, 634, 851
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 3 cut(s) 425, 437, 769
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 2 cut(s) 311, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
HpyF3I CTNAG 3 cut(s) 519, 551, 640
HpySE526I ACGT 1 cut(s) 685
HspAI GCGC 1 cut(s) 929
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 2 cut(s) 427, 847
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 3 cut(s) 118, 151, 377
LpnPI CCDG 8 cut(s) 15, 42, 129, 225, 453, 515, 586, 621
Lsp1109I GCAGC 4 cut(s) 323, 406, 409, 478
LweI GCATC 1 cut(s) 606
MabI ACCWGGT 1 cut(s) 28
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 3 cut(s) 546, 650, 686
MalI GATC 2 cut(s) 429, 849
MboI GATC 2 cut(s) 427, 847
MboII GAAGA 9 cut(s) 35, 148, 193, 273, 395, 704, 839, 842, 848
MflI RGATCY 1 cut(s) 847
MhlI GDGCHC 3 cut(s) 8, 156, 279
MluCI AATT 4 cut(s) 137, 336, 507, 716
MlyI GAGTC 1 cut(s) 881
MseI TTAA 6 cut(s) 69, 90, 161, 362, 777, 903
MslI CAYNNNNRTG 3 cut(s) 191, 675, 938
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
NcoI CCATGG 3 cut(s) 192, 291, 670
NdeI CATATG 1 cut(s) 909
NdeII GATC 2 cut(s) 427, 847
NlaIV GGNNCC 5 cut(s) 62, 244, 379, 438, 668
NmuCI GTSAC 3 cut(s) 546, 650, 686
NspI RCATGY 2 cut(s) 23, 460
PagI TCATGA 1 cut(s) 633
PciSI GCTCTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 709, 860
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 4 cut(s) 313, 396, 399, 468
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
PpuMI RGGWCCY 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 5 cut(s) 62, 244, 379, 438, 668
PspPI GGNCC 3 cut(s) 209, 437, 475
PspPPI RGGWCCY 1 cut(s) 437
PstI CTGCAG 1 cut(s) 468
PsuI RGATCY 1 cut(s) 847
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
RseI CAYNNNNRTG 3 cut(s) 191, 675, 938
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 6 cut(s) 69, 90, 161, 362, 777, 903
SatI GCNGC 4 cut(s) 312, 395, 398, 467
Sau3AI GATC 2 cut(s) 427, 847
Sau96I GGNCC 3 cut(s) 209, 437, 475
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 3 cut(s) 8, 156, 279
SexAI ACCWGGT 1 cut(s) 28
SfaNI GCATC 1 cut(s) 606
SfcI CTRYAG 1 cut(s) 464
SinI GGWCC 2 cut(s) 437, 475
SmiMI CAYNNNNRTG 3 cut(s) 191, 675, 938
Sse9I AATT 4 cut(s) 137, 336, 507, 716
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 6 cut(s) 192, 291, 373, 565, 670, 702
TaaI ACNGT 1 cut(s) 228
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 231, 330
TasI AATT 4 cut(s) 137, 336, 507, 716
TfiI GAWTC 2 cut(s) 709, 860
Tru1I TTAA 6 cut(s) 69, 90, 161, 362, 777, 903
Tru9I TTAA 6 cut(s) 69, 90, 161, 362, 777, 903
TseFI GTSAC 3 cut(s) 546, 650, 686
TseI GCWGC 4 cut(s) 311, 394, 397, 466
Tsp45I GTSAC 3 cut(s) 546, 650, 686
TspDTI ATGAA 6 cut(s) 193, 583, 622, 650, 798, 849
TspGWI ACGGA 1 cut(s) 860
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 437, 475
XapI RAATTY 1 cut(s) 137
XceI RCATGY 2 cut(s) 23, 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.