MD03G1010300.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013228
Physical Location & Seq
Reverse (-)
790344 .. 791294
951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010300.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGCACTTTAGTTGGACATGTAACACCAGGCCTTGGGTTTCTTCTCATTGGCTTATGGCACCTCTTCAACCACATTAAACTCCATCTTTACCAGCCAAACTCCTACGCATCTCCAACCTGGTTTCCCACCACCAAATTCAAGTATTTGGAGCCCTTCTTAATCATGGGAGGCTCATTTGCCTTCATTGCAACGGAACTGTTCATAGCCCCGGAGAAACACCAACCCTTCGATGACGATGGAACCATCCCCACCAACCGTCTCCGCAACTTTGAGCACTGTTCAATCTCCATGGCCTTCTTGGTCTATGCAGCTTTTGCTATCATTCTTGACAAAATTGCCCCAAAAGCACAACATGGTTTGACCCAACTCCTTGGAGCCATAGCCTTTGGGCAGCAGCTTCTTCTCTTTCACCTTCACTCTACTGATCACATGGGACCTGAAGGCCAATACCACATGCTTCTGCAGCTTGTGGTTCTTGTTTCTTTGGCCACAACCCTTATGGGAATTGGTTTCCCTAAGAGTTTTTTGGTCAGCTTTGTGAGGTCACTTAGCATATTTTCCCAAGGTGCTTGGCTTATTGTCATGGGGTTCATGCTTTGGACACCTGAATTGATGCCAAAAGGTTGCTTCATGAACTTAGAGGACGATCCCAAAGTGGTTACGTGCCATGGAGATGAGGCACTTCACAGAGCCAAGTCCTTAGTGAACCTCGAATTTAGTTGGTTCTTGGTGGGAATAGCCATTTTTGGGGTGTCCTTCTATTTGGTTTTGGTTAGGGTTTACAGTGAAAAGGTTGAGTATTCTTCATTGACAAGGCTACGCGAAGAAGATGAGGAGGAACCGAATCTTGATGTTGAATCTCAAAAGAAAAGTAAGCTTGGAGAGACAGAGAGTTTTGTTAGTCATATGGGAAAAACAGCCTTTGCGTCCATGGATATGGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

317

Amino Acids

35.59

Weight (kDa)

5.94

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 120 - 256 4.2e-52 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55230
fragaria_vesca FvH4_3g44810
malus_domestica MD03G1010300.v1.1 MD03G1010400.v1.1 MD11G1012300.v1.1
prunus_persica Prupe.6G010200_v2.0.a1
pyrus_communis pycom03g00920 pycom11g00800
rosa_chinensis RchiOBHm_Chr5g0081481
rosa_laevigata RLG00000036942
rosa_multiflora Rmu_sc0001783.1_g000010
rosa_roxburghii Rroxscaffold_1G00002050
rosa_rugosa Rorug05G0476700
rosa_samantha Rh5AG528200 Rh5BG552800 Rh5CG575600 Rh5DG563000
rosa_wichuraiana Rw5G049130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 60
AccB7I CCANNNNNTGG 1 cut(s) 35
AccII CGCG 1 cut(s) 825
AciI CCGC 1 cut(s) 265
AclWI GGATC 1 cut(s) 644
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 2 cut(s) 137, 716
AcuI CTGAAG 1 cut(s) 462
AfiI CCNNNNNNNGG 2 cut(s) 35, 750
AflIII ACRYGT 1 cut(s) 19
AgsI TTSAA 4 cut(s) 70, 142, 285, 860
AjnI CCWGG 2 cut(s) 28, 119
AluBI AGCT 5 cut(s) 314, 400, 469, 537, 880
AluI AGCT 5 cut(s) 314, 400, 469, 537, 880
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 2 cut(s) 266, 881
AlwI GGATC 1 cut(s) 644
AoxI GGCC 4 cut(s) 31, 294, 445, 489
ApeKI GCWGC 4 cut(s) 311, 394, 397, 466
ApoI RAATTY 2 cut(s) 137, 716
AspS9I GGNCC 1 cut(s) 437
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 1 cut(s) 404
AvaII GGWCC 1 cut(s) 437
BaeGI GKGCMC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 60
BanII GRGCYC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 4 cut(s) 323, 406, 409, 478
BccI CCATC 3 cut(s) 93, 233, 254
BciT130I CCWGG 2 cut(s) 30, 121
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 266, 881
BfmI CTRYAG 1 cut(s) 464
BisI GCNGC 4 cut(s) 312, 395, 398, 467
BlsI GCNGC 4 cut(s) 313, 396, 399, 468
Bme1390I CCNGG 3 cut(s) 30, 121, 212
Bme18I GGWCC 1 cut(s) 437
BmgT120I GGNCC 1 cut(s) 437
BmiI GGNNCC 6 cut(s) 62, 153, 244, 379, 438, 843
BmrFI CCNGG 3 cut(s) 30, 121, 212
BmsI GCATC 2 cut(s) 119, 606
BpuMI CCSGG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 666
BsaJI CCNNGG 7 cut(s) 34, 210, 291, 373, 565, 670, 933
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 750
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 30, 121
BseDI CCNNGG 7 cut(s) 34, 210, 291, 373, 565, 670, 933
BseGI GGATG 1 cut(s) 246
BseLI CCNNNNNNNGG 2 cut(s) 35, 750
BseMI GCAATG 1 cut(s) 186
BseRI GAGGAG 1 cut(s) 851
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 4 cut(s) 323, 406, 409, 478
Bsh1236I CGCG 1 cut(s) 825
BshFI GGCC 4 cut(s) 33, 296, 447, 491
BshNI GGYRCC 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 279
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 35, 750
BsmAI GTCTC 2 cut(s) 266, 881
BsmBI CGTCTC 1 cut(s) 266
BsmFI GGGAC 1 cut(s) 450
BsnI GGCC 4 cut(s) 33, 296, 447, 491
Bsp1286I GDGCHC 3 cut(s) 8, 156, 279
Bsp143I GATC 2 cut(s) 427, 649
Bsp19I CCATGG 3 cut(s) 291, 670, 933
BspACI CCGC 1 cut(s) 265
BspANI GGCC 4 cut(s) 33, 296, 447, 491
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 6 cut(s) 62, 153, 244, 379, 438, 843
BspMAI CTGCAG 1 cut(s) 468
BspPI GGATC 1 cut(s) 644
BspT107I GGYRCC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 7 cut(s) 34, 210, 291, 373, 565, 670, 933
BssMI GATC 2 cut(s) 427, 649
BssT1I CCWWGG 6 cut(s) 34, 291, 373, 565, 670, 933
Bst2UI CCWGG 2 cut(s) 30, 121
Bst4CI ACNGT 4 cut(s) 201, 260, 281, 788
Bst6I CTCTTC 1 cut(s) 71
BstAPI GCANNNNNTGC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 666
BstDEI CTNAG 4 cut(s) 519, 551, 640, 703
BstDSI CCRYGG 3 cut(s) 291, 670, 933
BstF5I GGATG 1 cut(s) 246
BstFNI CGCG 1 cut(s) 825
BstKTI GATC 2 cut(s) 430, 652
BstMAI GTCTC 2 cut(s) 266, 881
BstMBI GATC 2 cut(s) 427, 649
BstMWI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
BstNI CCWGG 2 cut(s) 30, 121
BstNSI RCATGY 2 cut(s) 23, 460
BstSCI CCNGG 3 cut(s) 28, 119, 210
BstSFI CTRYAG 1 cut(s) 464
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 4 cut(s) 323, 406, 409, 478
BstXI CCANNNNNNTGG 2 cut(s) 374, 940
BsuRI GGCC 4 cut(s) 33, 296, 447, 491
BtgI CCRYGG 3 cut(s) 291, 670, 933
BtsCI GGATG 1 cut(s) 246
BtsIMutI CAGTG 2 cut(s) 277, 793
CciI TCATGA 1 cut(s) 633
Cfr13I GGNCC 1 cut(s) 437
CseI GACGC 1 cut(s) 918
CsiI ACCWGGT 1 cut(s) 119
DdeI CTNAG 4 cut(s) 519, 551, 640, 703
DpnI GATC 2 cut(s) 429, 651
DpnII GATC 2 cut(s) 427, 649
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 71
EarI CTCTTC 1 cut(s) 71
Eco130I CCWWGG 6 cut(s) 34, 291, 373, 565, 670, 933
Eco147I AGGCCT 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 1 cut(s) 437
Eco57I CTGAAG 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 2 cut(s) 28, 119
EcoT14I CCWWGG 6 cut(s) 34, 291, 373, 565, 670, 933
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 6 cut(s) 34, 291, 373, 565, 670, 933
Esp3I CGTCTC 1 cut(s) 266
FaqI GGGAC 1 cut(s) 450
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 4 cut(s) 312, 395, 398, 467
FokI GGATG 1 cut(s) 233
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 4 cut(s) 312, 395, 398, 467
GluI GCNGC 4 cut(s) 312, 395, 398, 467
HaeIII GGCC 4 cut(s) 33, 296, 447, 491
HapII CCGG 1 cut(s) 212
HgaI GACGC 1 cut(s) 918
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 2 cut(s) 847, 860
HpaII CCGG 1 cut(s) 212
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 709, 784
Hpy188III TCNNGA 3 cut(s) 329, 634, 851
Hpy8I GTNNAC 2 cut(s) 709, 784
HpyAV CCTTC 7 cut(s) 166, 193, 238, 307, 425, 437, 769
HpyCH4III ACNGT 4 cut(s) 201, 260, 281, 788
HpyCH4IV ACGT 1 cut(s) 665
HpyCH4V TGCA 3 cut(s) 191, 311, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
HpyF3I CTNAG 4 cut(s) 519, 551, 640, 703
HpySE526I ACGT 1 cut(s) 665
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 2 cut(s) 427, 649
LmnI GCTCC 2 cut(s) 151, 377
LpnPI CCDG 8 cut(s) 15, 42, 106, 107, 133, 225, 453, 621
Lsp1109I GCAGC 4 cut(s) 323, 406, 409, 478
LweI GCATC 2 cut(s) 119, 606
MabI ACCWGGT 1 cut(s) 119
MaeII ACGT 1 cut(s) 665
MaeIII GTNAC 3 cut(s) 22, 546, 661
MalI GATC 2 cut(s) 429, 651
MboI GATC 2 cut(s) 427, 649
MboII GAAGA 6 cut(s) 35, 58, 395, 798, 839, 842
MhlI GDGCHC 3 cut(s) 8, 156, 279
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 5 cut(s) 137, 336, 507, 611, 716
MluNI TGGCCA 1 cut(s) 491
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 8 cut(s) 74, 164, 537, 637, 673, 722, 829, 832
Mox20I TGGCCA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 491
MseI TTAA 2 cut(s) 78, 161
MslI CAYNNNNRTG 2 cut(s) 675, 938
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 3 cut(s) 30, 121, 212
MvaI CCWGG 2 cut(s) 30, 121
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 4 cut(s) 188, 317, 347, 466
NciI CCSGG 1 cut(s) 212
NcoI CCATGG 3 cut(s) 291, 670, 933
NdeI CATATG 1 cut(s) 909
NdeII GATC 2 cut(s) 427, 649
NlaIV GGNNCC 6 cut(s) 62, 153, 244, 379, 438, 843
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 2 cut(s) 23, 460
PagI TCATGA 1 cut(s) 633
PceI AGGCCT 1 cut(s) 33
PciI ACATGT 1 cut(s) 19
PfeI GAWTC 2 cut(s) 847, 860
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 4 cut(s) 313, 396, 399, 468
Ppu21I YACGTR 1 cut(s) 666
PpuMI RGGWCCY 1 cut(s) 437
PscI ACATGT 1 cut(s) 19
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 28, 119
PspGI CCWGG 2 cut(s) 28, 119
PspN4I GGNNCC 6 cut(s) 62, 153, 244, 379, 438, 843
PspPI GGNCC 1 cut(s) 437
PspPPI RGGWCCY 1 cut(s) 437
PstI CTGCAG 1 cut(s) 468
RseI CAYNNNNRTG 2 cut(s) 675, 938
SaqAI TTAA 2 cut(s) 78, 161
SatI GCNGC 4 cut(s) 312, 395, 398, 467
Sau3AI GATC 2 cut(s) 427, 649
Sau96I GGNCC 1 cut(s) 437
ScrFI CCNGG 3 cut(s) 30, 121, 212
SduI GDGCHC 3 cut(s) 8, 156, 279
SexAI ACCWGGT 1 cut(s) 119
SfaNI GCATC 2 cut(s) 119, 606
SfcI CTRYAG 1 cut(s) 464
SinI GGWCC 1 cut(s) 437
SmiMI CAYNNNNRTG 2 cut(s) 675, 938
Sse9I AATT 5 cut(s) 137, 336, 507, 611, 716
SseBI AGGCCT 1 cut(s) 33
SsiI CCGC 1 cut(s) 265
StuI AGGCCT 1 cut(s) 33
StyD4I CCNGG 3 cut(s) 28, 119, 210
StyI CCWWGG 6 cut(s) 34, 291, 373, 565, 670, 933
TaaI ACNGT 4 cut(s) 201, 260, 281, 788
TaiI ACGT 1 cut(s) 668
TaqI TCGA 2 cut(s) 231, 714
TasI AATT 5 cut(s) 137, 336, 507, 611, 716
TfiI GAWTC 2 cut(s) 847, 860
Tru1I TTAA 2 cut(s) 78, 161
Tru9I TTAA 2 cut(s) 78, 161
TscAI CASTG 2 cut(s) 284, 793
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 4 cut(s) 311, 394, 397, 466
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 6 cut(s) 175, 193, 583, 622, 650, 798
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 284, 793
Van91I CCANNNNNTGG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 437
XapI RAATTY 2 cut(s) 137, 716
XceI RCATGY 2 cut(s) 23, 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.