MD11G1012300.v1.1

Family of unknown function (DUF716)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013228
Physical Location & Seq
Reverse (-)
886298 .. 887248
951 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1012300.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGGCACTTTAGTTGGGCATGTAGCACCGGGCTTTGGGTTTCTTCTCATTGGATTATGGCACCTCTTTAATCACATCAAACTCCATCTTAACCAACCAAACACCTACACAGCTCCATCCTGGTTTCCCACCTCCAAATTCAAGTATTTGGAGCTCTTCTTAATCATGGGAGGCTCATCTGCATCCATTGCCATGGAACTCTTCATAGGTCCACAGAAACACCGACCGCTCGATGACGATGGAACGATCCCCTCCAACCATCTCCACAACTTCGAGCACTCTTCAATCTCCATGACTTTCTTGGTCTATGCAGCTTTTGCCATCGTTCTTGACAAAATTTCCCCAAAAGCACAACATGGTTTGACCCAACTCCTTGGAGCCATAGCGTTTGGGCAGCAGCTTCTTCTCTTTCACCTTCACTCTGCTGATCACATGGGACCTGAAGGTCAATACCACATGCTTCTACAGCTTGTGGTTCTTGTTTCTTTGACCACAACCCTTATGGGAATTGGTTTGCCTGAGAGTTTTTTGGTCAGCTTTGTGAGGTCACTAAGCATATTTTTCCAAGGTGTTTGGCTTATGGTCATGGGGTTCATGCTTTGGACACCTGATTTGATGCCAAAAGGTTGTTCCATGAACTCAGAGGAGGGTCACAAAGTGGTTAGGTGCCATGGAGATGAGGCACTTCACAGAGCCAAATCCTTAGTGAACCTTCAATTTAGTTGGTTCTTGTTGGGAGTTGCCATTTTTGGGGTGTCCTTCTATTTGGTTTTGGTTAAGGTTTATGGCGAAAAGTTTGAGTATTTTTCATTGACCAGACTACGCGAAGAAGATGAAGAGGATCTGGATCATGATGTTGAATATCAAAAGAAAAGTAAGCTTGAAGACTCGAAGAGTTTTGTTAATCATATGGGAAAAACAGCTTTTGCGTCCATAGACATTGAAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF716

Protein Analysis

317

Amino Acids

35.65

Weight (kDa)

6.2

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF716 PF04819 120 - 256 8e-55 Family of unknown function (DUF716)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013228)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55230
fragaria_vesca FvH4_3g44810
malus_domestica MD03G1010300.v1.1 MD03G1010400.v1.1 MD11G1012300.v1.1
prunus_persica Prupe.6G010200_v2.0.a1
pyrus_communis pycom03g00920 pycom11g00800
rosa_chinensis RchiOBHm_Chr5g0081481
rosa_laevigata RLG00000036942
rosa_multiflora Rmu_sc0001783.1_g000010
rosa_roxburghii Rroxscaffold_1G00002050
rosa_rugosa Rorug05G0476700
rosa_samantha Rh5AG528200 Rh5BG552800 Rh5CG575600 Rh5DG563000
rosa_wichuraiana Rw5G049130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 444
AccB1I GGYRCC 2 cut(s) 60, 666
AccBSI CCGCTC 1 cut(s) 229
AccII CGCG 1 cut(s) 825
AciI CCGC 1 cut(s) 227
AclWI GGATC 3 cut(s) 241, 849, 855
AcsI RAATTY 2 cut(s) 137, 336
AcuI CTGAAG 1 cut(s) 462
AdeI CACNNNGTG 1 cut(s) 658
AfiI CCNNNNNNNGG 2 cut(s) 35, 750
AgsI TTSAA 6 cut(s) 142, 285, 716, 860, 884, 944
AjnI CCWGG 1 cut(s) 119
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 8 cut(s) 113, 154, 314, 400, 469, 537, 880, 923
AluI AGCT 8 cut(s) 113, 154, 314, 400, 469, 537, 880, 923
Alw21I GWGCWC 2 cut(s) 156, 279
AlwI GGATC 3 cut(s) 241, 849, 855
ApeKI GCWGC 3 cut(s) 311, 394, 397
ApoI RAATTY 2 cut(s) 137, 336
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
AspS9I GGNCC 2 cut(s) 209, 437
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 404
AvaII GGWCC 2 cut(s) 209, 437
BaeGI GKGCMC 1 cut(s) 8
BanI GGYRCC 2 cut(s) 60, 666
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 2 cut(s) 156, 279
BbvI GCAGC 3 cut(s) 323, 406, 409
BccI CCATC 5 cut(s) 93, 124, 233, 267, 329
BciT130I CCWGG 1 cut(s) 121
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 1 cut(s) 30
BfmI CTRYAG 1 cut(s) 464
BisI GCNGC 3 cut(s) 312, 395, 398
BlsI GCNGC 3 cut(s) 313, 396, 399
Bme1390I CCNGG 2 cut(s) 30, 121
Bme18I GGWCC 2 cut(s) 209, 437
BmgT120I GGNCC 2 cut(s) 209, 437
BmiI GGNNCC 4 cut(s) 62, 379, 438, 668
BmrFI CCNGG 2 cut(s) 30, 121
BmsI GCATC 2 cut(s) 191, 606
BpiI GAAGAC 1 cut(s) 891
BpuMI CCSGG 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 846
BsaJI CCNNGG 4 cut(s) 192, 373, 565, 670
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 750
Bse3DI GCAATG 1 cut(s) 186
Bse8I GATNNNNATC 1 cut(s) 846
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 4 cut(s) 192, 373, 565, 670
BseGI GGATG 2 cut(s) 116, 182
BseJI GATNNNNATC 1 cut(s) 846
BseLI CCNNNNNNNGG 2 cut(s) 35, 750
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 2 cut(s) 510, 654
BseRI GAGGAG 1 cut(s) 659
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 3 cut(s) 323, 406, 409
Bsh1236I CGCG 1 cut(s) 825
Bsh1285I CGRYCG 1 cut(s) 227
BshNI GGYRCC 2 cut(s) 60, 666
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 2 cut(s) 156, 279
BsiSI CCGG 1 cut(s) 29
BslFI GGGAC 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 35, 750
BsmFI GGGAC 1 cut(s) 450
Bsp1286I GDGCHC 3 cut(s) 8, 156, 279
Bsp143I GATC 4 cut(s) 246, 427, 841, 847
Bsp19I CCATGG 2 cut(s) 192, 670
BspACI CCGC 1 cut(s) 227
BspCNI CTCAG 2 cut(s) 511, 653
BspFNI CGCG 1 cut(s) 825
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 4 cut(s) 62, 379, 438, 668
BspPI GGATC 3 cut(s) 241, 849, 855
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 2 cut(s) 60, 666
BsrBI CCGCTC 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 186
BssECI CCNNGG 4 cut(s) 192, 373, 565, 670
BssMI GATC 4 cut(s) 246, 427, 841, 847
BssT1I CCWWGG 4 cut(s) 192, 373, 565, 670
Bst2UI CCWGG 1 cut(s) 121
Bst6I CTCTTC 5 cut(s) 161, 206, 286, 831, 887
BstAPI GCANNNNNTGC 2 cut(s) 188, 317
BstDEI CTNAG 4 cut(s) 519, 551, 640, 703
BstDSI CCRYGG 2 cut(s) 192, 670
BstF5I GGATG 2 cut(s) 116, 182
BstFNI CGCG 1 cut(s) 825
BstKTI GATC 4 cut(s) 249, 430, 844, 850
BstMBI GATC 4 cut(s) 246, 427, 841, 847
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 3 cut(s) 188, 317, 466
BstNI CCWGG 1 cut(s) 121
BstNSI RCATGY 2 cut(s) 23, 460
BstSCI CCNGG 2 cut(s) 28, 119
BstSFI CTRYAG 1 cut(s) 464
BstSLI GKGCMC 1 cut(s) 8
BstUI CGCG 1 cut(s) 825
BstV1I GCAGC 3 cut(s) 323, 406, 409
BstV2I GAAGAC 1 cut(s) 891
BstX2I RGATCY 1 cut(s) 841
BstXI CCANNNNNNTGG 2 cut(s) 193, 374
BstYI RGATCY 1 cut(s) 841
BtgI CCRYGG 2 cut(s) 192, 670
BtsCI GGATG 2 cut(s) 116, 182
CciI TCATGA 1 cut(s) 850
Cfr13I GGNCC 2 cut(s) 209, 437
CseI GACGC 1 cut(s) 918
DdeI CTNAG 4 cut(s) 519, 551, 640, 703
DpnI GATC 4 cut(s) 248, 429, 843, 849
DpnII GATC 4 cut(s) 246, 427, 841, 847
DraIII CACNNNGTG 1 cut(s) 658
DrdI GACNNNNNNGTC 1 cut(s) 444
DseDI GACNNNNNNGTC 1 cut(s) 444
Eam1104I CTCTTC 5 cut(s) 161, 206, 286, 831, 887
EarI CTCTTC 5 cut(s) 161, 206, 286, 831, 887
Ecl136II GAGCTC 1 cut(s) 154
Eco130I CCWWGG 4 cut(s) 192, 373, 565, 670
Eco24I GRGCYC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 209, 437
Eco53kI GAGCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 154
EcoO109I RGGNCCY 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 4 cut(s) 192, 373, 565, 670
EcoT38I GRGCYC 1 cut(s) 156
ErhI CCWWGG 4 cut(s) 192, 373, 565, 670
FaqI GGGAC 1 cut(s) 450
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 3 cut(s) 312, 395, 398
FokI GGATG 2 cut(s) 103, 169
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 3 cut(s) 312, 395, 398
GluI GCNGC 3 cut(s) 312, 395, 398
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 918
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 1 cut(s) 887
HpaII CCGG 1 cut(s) 29
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 212, 709
Hpy188I TCNGA 1 cut(s) 643
Hpy188III TCNNGA 3 cut(s) 329, 845, 851
Hpy8I GTNNAC 2 cut(s) 212, 709
HpyAV CCTTC 4 cut(s) 425, 437, 722, 769
HpyCH4V TGCA 2 cut(s) 182, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 317, 466
HpyF3I CTNAG 4 cut(s) 519, 551, 640, 703
Ksp22I TGATCA 1 cut(s) 427
Kzo9I GATC 4 cut(s) 246, 427, 841, 847
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 3 cut(s) 118, 151, 377
LpnPI CCDG 8 cut(s) 42, 106, 133, 453, 531, 621, 829, 830
Lsp1109I GCAGC 3 cut(s) 323, 406, 409
LweI GCATC 2 cut(s) 191, 606
MaeIII GTNAC 2 cut(s) 546, 650
MalI GATC 4 cut(s) 248, 429, 843, 849
MbiI CCGCTC 1 cut(s) 229
MboI GATC 4 cut(s) 246, 427, 841, 847
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 3 cut(s) 8, 156, 279
MluCI AATT 4 cut(s) 137, 336, 507, 716
MlyI GAGTC 1 cut(s) 881
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 9 cut(s) 74, 142, 164, 262, 537, 637, 640, 673, 832
MseI TTAA 5 cut(s) 69, 90, 161, 777, 903
MslI CAYNNNNRTG 2 cut(s) 191, 675
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 2 cut(s) 30, 121
MvaI CCWGG 1 cut(s) 121
MvnI CGCG 1 cut(s) 825
MwoI GCNNNNNNNGC 3 cut(s) 188, 317, 466
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 2 cut(s) 192, 670
NdeI CATATG 1 cut(s) 909
NdeII GATC 4 cut(s) 246, 427, 841, 847
NlaIV GGNNCC 4 cut(s) 62, 379, 438, 668
NmuCI GTSAC 2 cut(s) 546, 650
NspI RCATGY 2 cut(s) 23, 460
PagI TCATGA 1 cut(s) 850
PciSI GCTCTTC 1 cut(s) 161
PkrI GCNGC 3 cut(s) 313, 396, 399
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
PpuMI RGGWCCY 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 4 cut(s) 62, 379, 438, 668
PspPI GGNCC 2 cut(s) 209, 437
PspPPI RGGWCCY 1 cut(s) 437
PsuI RGATCY 1 cut(s) 841
RseI CAYNNNNRTG 2 cut(s) 191, 675
SacI GAGCTC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 5 cut(s) 69, 90, 161, 777, 903
SatI GCNGC 3 cut(s) 312, 395, 398
Sau3AI GATC 4 cut(s) 246, 427, 841, 847
Sau96I GGNCC 2 cut(s) 209, 437
SchI GAGTC 1 cut(s) 881
ScrFI CCNGG 2 cut(s) 30, 121
SduI GDGCHC 3 cut(s) 8, 156, 279
SfaNI GCATC 2 cut(s) 191, 606
SfcI CTRYAG 1 cut(s) 464
SinI GGWCC 2 cut(s) 209, 437
SmiMI CAYNNNNRTG 2 cut(s) 191, 675
Sse9I AATT 4 cut(s) 137, 336, 507, 716
SsiI CCGC 1 cut(s) 227
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 2 cut(s) 28, 119
StyI CCWWGG 4 cut(s) 192, 373, 565, 670
TaqI TCGA 3 cut(s) 231, 273, 890
TasI AATT 4 cut(s) 137, 336, 507, 716
Tru1I TTAA 5 cut(s) 69, 90, 161, 777, 903
Tru9I TTAA 5 cut(s) 69, 90, 161, 777, 903
TseFI GTSAC 2 cut(s) 546, 650
TseI GCWGC 3 cut(s) 311, 394, 397
Tsp45I GTSAC 2 cut(s) 546, 650
TspDTI ATGAA 5 cut(s) 193, 583, 650, 798, 849
VpaK11BI GGWCC 2 cut(s) 209, 437
XapI RAATTY 2 cut(s) 137, 336
XceI RCATGY 2 cut(s) 23, 460
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.